Investigation of the status of rest facilities at industrial sites and awareness of relevant laws and regulations of South Korea.

South Korea has faced many social issues due to long working hours, lack of rest areas, and poor rest facility environments for cleaners, security guards, department store workers, etc. Discussions have been ongoing about mandating the installation of rest facilities. From August 18, 2022, Article 128-2 of the Occupational Safety and Health Act, concerning the installation of rest facilities, was enforced. Consequently, employers in all industries are required to install rest facilities, and laws have been established to ensure these facilities meet certain standards. Accordingly, this study investigated the current status of rest facility installations and the awareness of the law's enactment in Korean industrial sites. The results, analyzed by gender, age, managerial status, industry, and size of the business, indicated that younger people were less satisfied with the rest facilities. Managers were more knowledgeable about the legal regulations than workers. In the service industry, compared to other industries, smaller businesses were less likely to have rest facilities and were less aware of the legal regulations. The results of this study are expected to be used as basic data to help establish the rest facility installation laws in Korea.

Noise exposure assessment of workers inspecting construction machinery vehicles in the Republic of Korea.

Previous studies have investigated the health hazards caused by exposure to the noise of heavy equipment used at construction sites. Korea's Ministry of Land, Infrastructure, and Transport operates a registration system for construction machinery, and regular safety inspections are mandatory. Although workers inspecting construction machinery vehicles are exposed to unwanted noise, no noise exposure assessment has been made regarding these workers in Korea. The time-weighted average (TWA) daily average noise exposure level among construction machinery inspectors at 18 inspection centers was 75.3 dB(A). Among the inspection steps, the exhaust gas inspection step was found to exhibit the highest noise exposure level, up to 100 dB(A). In Korea, if the noise level of a workplace exceeds 85 dB(A) as a TWA, workers are required to undergo special medical examinations. This study found that special medical examinations were required for two of the 18 target inspection centers (approximately 115 workers) as the 8-hour TWA noise level exceeded 85 dB(A). Therefore, regular noise exposure assessment and special medical examinations for noise are required to prevent inspectors from developing hearing disorders due to noise exposure.

Formaldehyde Exposure of Aquaculture Workers in Korea.

OBJECTIVES: Korea's aquaculture sector primarily cultivates aquatic life, with fish seed production as a focus. Formalin, a parasiticide, consists of 37% formaldehyde mixed with yellow No. 4 dye. Formaldehyde vaporization poses cancer risks, classified as a carcinogen. Korea regulates formaldehyde as a hazardous substance, requiring workplace environment measurements. Few aquaculture farms have conducted these checks in recent years. In this study, we investigated actual formaldehyde exposure levels among Korean aquaculture workers, highlighting a critical safety concern. METHODS: A field survey was conducted to measure formaldehyde exposure at 10 aquaculture farms in areas where Korean aquaculture is concentrated. Short-term and long-term personal samples, local samples, and direct-reading measurements were conducted. Formaldehyde exposure levels were detected in short-term personal samples from six farms and in long-term personal samples from two farms, and formaldehyde was detected in all local samples. In direct-reading measurements, a high concentration of formaldehyde was sustained for short periods. RESULTS: Long-term (8-hour) personal samples were mostly non-detectable, except for farms A and D, which had levels of 0.0009 ppm and 0.0017 ppm, respectively. Short-term (15-minute) samples were non-detectable in four farms, with an average of 0.0158 (±0.0130) ppm in the remaining six farms. Local samples from all farms had an average of 0.0384 (±0.0957) ppm of formaldehyde. For farms A and D, where long-term sampling detected formaldehyde, real-time measurements showed a sustained high concentration in farm A for about 48 minutes before decreasing. Farm D had no detectable formaldehyde throughout the monitoring period. CONCLUSION: According to the formaldehyde exposure level assessment, short term exposure to formaldehyde during and immediately after application of formalin nearly exceeded the ACGIH TLV STEL in one farm. However, concentration of long term samples appeared at 10% of ACGIH TLV TWA. Additional study is recommended to determine whether exposure to formaldehyde poses a health risk for aquaculture workers during application of formalin.

Effectiveness of a Participatory Program for Improving the Cardiovascular and Cerebrovascular Health of Older Farmers in Rural Korea.

BACKGROUND: The aim of this study was to evaluate the effectiveness of a participatory approach to the cardiovascular and cerebrovascular (CCV) health of older farmers in rural Korea. METHODS: A nonequivalent control group pretest-posttest design was used. Participants included 58 farmers aged ≥ 60 years who were assigned to either an experimental group (n = 28) or a comparative group (n = 30). The experimental group took part in the participatory program for CCV health, while the comparative group received a conventional lecture program for CCV health. The two groups, from pretest to posttest, were compared using the generalized estimating equation (GEE) method. RESULTS: The participatory program showed a greater effect over time than the conventional lecture program for health empowerment (Χ2 = 7.92, p = 0.005) and self-efficacy in managing CCV health (Χ2 = 5.94, p = 0.015). The average rate of implemented improvements after 3 months was 88.9%, showing that the participatory program was successful. CONCLUSIONS: The participatory program for CCV health was an effective intervention for the empowerment and self-efficacy of older farmers in managing their own CCV health. Therefore, we recommend replacing lectures with participatory methods in CCV health programs for older farmers.

Benzene exposure assessment of printing workers treating petroleum-based cleaner in South Korea.

This study was conducted to check whether benzene is contained inside the petroleum-based cleaning agent used in the printing industry and measure whether it is actually exposed to the air. Benzene was analyzed inside the cleaning agent and air exposure evaluation was done by area sampling. Risk assessment was performed using the Chemical Hazard Risk Management (CHARM) technique. Most products contained benzene based on the results obtained from this study. As a result of collecting air samples and checking whether the workers were exposed to benzene actually, benzene was detected in three samples. As a result of the risk assessment, most of printing businesses scored more than four points. Benzene was detected in all petroleum-based cleaning products. In addition, benzene was detected in some of air samples. Considering the fact that even small exposure level of benzene is dangerous to worker health and most of the printing businesses in South Korea operate on a small scale with fewer than five employees so the health management system is poor, it is necessary to prepare appropriate measures to prevent work diseases provoked by benzene exposure.

Exposure Assessment of Airborne Bacteria and Fungi in the Aircraft.

Objective: The exposure levels of disease-causing bacteria and germs were assessed on aircraft cleaning workers on multiple different aircrafts. Method: Five measuring points were selected depending on the aircraft types. Four aircraft cleaning agencies were selected for the test. Aircraft cleaning work was classified as intensive cleaning and general cleaning work. Ventilation in aircraft when sampling during the cleaning operation was categorized into forced ventilation and natural ventilation. The collection of airborne microorganisms was made through inertial impactors which were installed 1.5 meters above the bottom of the aircraft. The airborne bacteria and fungus growth badges were selected by Trytpic Soy Agar and Sabouraud Dextrose Agar. Results: The average concentrations of bacteria in the air were higher in the order of small, medium, and large airplanes. Rainy days had higher concentrations inside and outside the aircraft as compared to those in sunny days. Regarding ventilation, concentrations in natural ventilation were higher than concentrations in forced ventilation. According to the type of work, the concentrations in the intensive cleaning groups (cleaning one plane a day) were lower than those of the ordinary cleaning groups (cleaning several planes per day). Conclusion: The concentration levels of airborne bacteria and fungi in the aircraft surveyed were lower than the indoor environmental standards of Korea (800 cfu/m3 and 500 cfu/m3). The average concentrations of bacteria in the air and fungi in the air were highest in small aircraft owned by Company D.

Estimating milk production losses by heat stress and its impacts on greenhouse gas emissions in Korean dairy farms.

Meteorological disasters caused by climate change like heat, cold waves, and unusually long rainy seasons affect the milk productivity of cows. Studies have been conducted on how milk productivity and milk compositions change due to heat stress (HS). However, the estimation of losses in milk production due to HS and hereby environmental impacts of greenhouse gas (GHG) emissions are yet to be evaluated in Korean dairy farms. Dairy milk production and milk compositions data from March to October 2018, provided by the Korea Dairy Committee (KDC), were used to compare regional milk production with the temperature-humidity index (THI). Raw data for the daily temperature and relative humidity in 2018 were obtained from the Korea Meteorological Administration (KMA). This data was used to calculate the THI and the difference between the maximum and minimum temperature changing rate, as the average daily temperature range, to show the extent to which the temperature gap can affect milk productivity. The amount of milk was calculated based on the price of 926 won/kg from KDC. The results showed that the average milk production rate was the highest within the THI range 60-73 in three regions in May: Chulwon (northern region), Hwasung (central region), and Gunwi (southern region). The average milk production decreased by 4.96 ± 1.48% in northern region, 7.12 ± 2.36% in central region, and 7.94 ± 2.57% in southern region from June to August, which had a THI range of 73 or more, when compared to May. Based on the results, the level of THI should be maintained like May. If so, the farmers can earn a profit of 9,128,730 won/farm in northern region, 9,967,880 won/farm in central region, and 12,245,300 won/farm in southern region. Additionally, the average number of cows raised can be reduced by 2.41 ± 0.35 heads/farm, thereby reducing GHG emissions by 29.61 ± 4.36 kg CO2eq/day on average. Overall, the conclusion suggests that maintaining environmental conditions in the summer that are similar to those in May is necessary. This knowledge can be used for basic research to persuade farmers to change farm facilities to increase the economic benefits and improve animal welfare.

Job Satisfaction Level of Safety and Health Manager in Construction Industry: Pandemic Period.

In a widespread social turmoil such as the Pandemic, job groups with high turnover rates and high job stress, such as the construction industry, will have a greater adverse impact than the general job group. This is to be used as basic data in preparing management plans by identifying the factors that hinder job stress and job satisfaction of construction workers. In this study, during the Pandemic period (1 September 2021 to 31 December 2021), a survey was conducted on job stress and job satisfaction among safety and health managers working at construction sites. The overall job satisfaction of workers in the construction industry was grasped by analyzing the level of correlation and the mutual influence on job stress, job satisfaction, general characteristics, and work-related characteristics. As a result, in terms of work characteristics, it was found that the smaller the working period in the current position, the more positive the job satisfaction was (p < 0.01). In addition, it was found that job satisfaction increased significantly when there was a promotion opportunity (p < 0.001). The construction industry is a job group with high basic job stress and low job satisfaction. In addition, it was evaluated that job stress increased during the pandemic.

Biological monitoring for exposure assessment of volatile organic compounds by Korean firefighters at the fire site.

The VOCs and metabolites in urine for exposed VOCs were evaluated for firefighters who participated in the actual fire fighting to determine whether firefighters were exposed to hazardous chemicals, which is the basic data on cancer risk of firefighters. When the fire extinguishing time is long, the concentration of benzene, PHEMA, and toluene among VOCs and metabolites in the case of fire suppression, rescue, and fire investigation work, which is estimated that the exposure of hazardous substances generated from the fire site at the time of fire suppression was large, significantly increased. In the case where the number of urination is 2 or less, the concentration of TZCA, toluene, and benzene among VOCs and metabolites was significantly increased compared to the number of urinating more than 2 times. In the concentration of VOCs and metabolites in urine corrected with creatinine, the concentrations of toluene and PHEMA in urine were significantly higher. The concentration of PHEMA in urine was higher in the group who participated in the fire suppression for more than 11 hours (long time) than the group who participated in the fire suppression for a short time.

Association between exposure level of air pollutants and incidence rate of circulatory disease in residential and industrial areas of South Korea.

This study investigated the correlation between the concentration of air pollutants in two metropolitan cities, Seoul and Incheon, located in South Korea with different urban characteristics and the number of patients with circulatory diseases among residents exposed to air pollution. The residential area was selected as Eunpyeong-gu of Seoul Metropolitan City and the industrial area as Jung-gu of Incheon Metropolitan City. The evaluation period is between 2015 and 2016. The relevant data provide by the Korea governmental agency were analysed to derive the purpose of this study. It was confirmed that PM10, PM2.5, nitrogen dioxide, carbon monoxide, and sulfur dioxide among air pollutants had an increasing impact on the incidence rate of circulatory diseases. The PM2.5 was positively correlated with the incidence rate of all circulatory diseases in residential area (p < 0.05). The carbon monoxide showed a positive correlation with circulatory system diseases except for hypertension in residential area. (p < 0.05). The sulfur dioxide was positively correlated with all circulatory diseases in both residential and industrial area (p < 0.05). Based on the results obtained from this study, it was found that there are different types of air pollutants that affect circulatory diseases in residential and industrial areas.

Effect of air cleaner on stress hormones of pig and pork quality.

The objective of this study is to investigate effect of air cleaner operated during pig breeding period on stress hormones of pigs and their pork quality. The stress hormones (cortisol, epinephrine and norepinephrine) in blood sample of pigs reared in the housing rooms with or without air cleaner have been measured according to a pig's rearing stage: 0 day (farrowing), 21st day (farrowing-weaning), 70th day (weaning-nursery), 140th day (nursery-growing), and 180th day (growing-fattening). The comparison of pork quality according to the application of an air cleaner was performed through the carcass analysis of the pigs shipped from swine house. The levels of cortisol, epinephrine, and norepinephrine in pigs reared in housing rooms with and without air cleaners were found to be within the range of normal reference values. Among pork quality evaluation items, the thickness of intermuscular fat and final carcass grade of pigs raised in housing room with air cleaner was generally superior to those of pigs raised in housing room without air cleaner (p < 0.05). Based on the results obtained from this study, it is concluded that air cleaner does not have a significant effect on reducing pig stress but contributes to improving pork quality in pig breeding.

Indoor distribution characteristics of airborne bacteria in pig buildings as influenced by season and housing type.

OBJECTIVE: A concentration of airborne bacteria generated from swine houses is recognized to be relatively higher than other work places and it is essential to optimally manage it to prevent farmers' respiratory diseases. This study was conducted to assess the distribution characteristics of airborne bacteria in swine houses located at South Korea. METHODS: A total 27 pig buildings of the enclosed type operated with mechanical ventilation system by a side wall fan and deep-pit manure system with slats were surveyed. Air samples were collected at 1.0 m above the middle floor in pig housing room. A six-stage viable particulate cascade impactor was used to identify the distribution of the sizes of particles in diameter. RESULTS: Seasonal mean levels of airborne bacteria in the housing rooms of gestation/farrowing pigs, nursery pigs and growing/fattening pigs were 3,428(±1,244) colony forming unit (cfu)/m3, 8,325(±3,209) cfu/m, and 13,254(±6,108) cfu/m3 for spring; 9,824(±2,157) cfu/m3, 18,254(±5,166) cfu/m3, and 24,088(±9,274) cfu/m3 for summer; 1,707(±957) cfu/m3, 4,258 (±1,438) cfu/m3, and 8,254(±2,416) cfu/m3 for autumn; and 2,322(±1,352) cfu/m3, 6,124(±1,527) cfu/m3 and 12,470(±4,869) cfu/m3 for winter, respectively. CONCLUSION: Concentrations of airborne bacteria according to pig housing type were highest in growing/fattening housing room followed by nursery housing room and gestation/farrowing housing room. In terms of seasonal aspect, the pig building showed the highest levels of airborne bacteria in summer followed by spring, winter and autumn. The respirable airborne bacteria which are ranged between 0.6 and 4.7 µm accounted for approximately 60% compared to total airborne bacteria regardless of pig housing type.

The level of submicron fungal fragments in homes with asthmatic children.

OBJECTIVES: Much scientific evidence indicates a positive association between moldy environments and respiratory illnesses and/or symptoms (e.g., asthma). Recently, submicron fungal fragments (<1.0 µm) have been suggested as a potential contributor to adverse health effects due to their biological composition (e.g., antigens, mycotoxins, and (1,3)-ß-D-glucan) as well as their small size. However, the contribution of exposure to fine fungal particles on adverse health outcomes has been poorly characterized, particularly in homes with asthmatic children. We characterized the airborne level of smaller-sized fungal particles between homes with and without asthmatic children. METHODS: We visited 29 homes with (n=15) and without (n=14) an asthmatic child and sampled submicron fungal fragments in a living room and child׳s bedroom, along with outdoor sampling, using the NIOSH two-stage sampler. (1,3)-ß-D-glucan of fungal fragments analyzed by Limulus Amebocyte lysate assay (LAL) was used for quantifying their exposure. RESULTS: Overall, the geometric mean (GM) concentration of (1,3)-ß-D-glucan in submicron fungal fragments in indoor air was two-fold higher in homes with asthmatic children (50.9 pg/m(3)) compared to homes with non-asthmatic children (26.7 pg/m(3)) (P<0.001). The GM concentration of these particles in child׳s bedroom in homes with an asthmatic child (66.1 pg/m(3)) was about three times higher than that in homes with non-asthmatic children (23.0 pg/m(3)) (P<0.001). The relative humidity had a negative correlation with the concentration of (1,3)-ß-D-glucan in submicron fungal fragments (Pearson coefficient=-0.257, P=0.046). CONCLUSIONS: Our findings indicate that homes with asthmatic children have a higher concentration of submicron fungal fragments compared to homes with non-asthmatic children. A greater exposure to smaller-sized fungal particles may occur in homes with an asthmatic child as relative humidity decreases. The very careful control of relative humidity in indoor air is necessary for reducing exposure to fine fungal particles and inhibiting the growth of microorganisms in homes with allergic diseases.

Information asymmetry, social networking site word of mouth, and mobility effects on social commerce in Korea.

The purpose of this research is to examine the issues that affect customers' behavioral character and purchasing behavior. The study proposes a research hypothesis with independent variables that include social presence, trust, and information asymmetry, and the dependent variable purchase decision making, to explain differentiated customer decision making processes in social commerce (S-commerce). To prove the hypothesis, positive verification was performed by focusing on mediating effects through a customer uncertainty variable and moderating effects through mobility and social networking site word of mouth (SNS WOM) variables. The number of studies on customer trends has rapidly increased together with the market size of S-commerce. However, few studies have examined the negative variables that make customers hesitant to make decisions in S-commerce. This study investigates the causes of customer uncertainty and focuses on deducing the control variables that offset this negative relationship. The study finds that in customers' S-commerce purchasing actions, the SNS WOM and mobility variables show control effects between information asymmetry and uncertainty and between trust and uncertainty. Additionally, this research defines the variables related to customer uncertainty that are hidden in S-commerce, and statistically verifies their relationship. The research results can be used in Internet marketing practices to establish marketing mix strategies for customer demand or as research data to predict customer behavior. The results are scientifically meaningful as a precedent for research on customers in S-commerce.

Effects of physical examination and diet consultation on serum cholesterol and health-behavior in the Korean pilots employed in commercial airline.

An objective of this study is to search how physical examination and diet consultation can influence those risk factors of cardiovascular disease. The subjects were 326 pilots of the "B" airline company in Korea whose total cholesterol values were over 220 mg/dl on their regular physical examinations from April 2006 to December 2008. They were divided into two groups, one who had diet consultation (an intervention group) and a control group. The physical examination components used to each group were body mass index (BMI), total cholesterol (TC), high density lipoprotein (HDL), low density lipoprotein (LDL) and triglyceride (TG). The behavioral, anthropometric and biomedical measurements were collected at each visit. This study compares and investigates the changes of serum cholesterol and also the health-behavior at each physical examination. Within the intervention group significant improvements were observed for total cholesterol, BMI (body mass index) and HDL (high density lipoprotein). The normalizing rates for cholesterol level to decrease down to lower than 200 mg/dl were 17.7% in intervention group and 8.7% in control group, which is statistically significantly higher among the intervention group. The odds ratio of diet consultation was 2.80 (95% CI=1.35-5.79), which indicates that it is a significantly contributing factor to normalize the serum cholesterol value down to lower than 200 mg/dl. Based on result, it is recommended to have regular physical examination and intensive management with diet and exercise consultation.

Exposure level and distribution characteristics of airborne bacteria and fungi in Seoul metropolitan subway stations.

The exposure level and distribution characteristics of airborne bacteria and fungi were assessed in the workers' activity areas (station office, bedroom, ticket office and driver's seat) and passengers' activity areas (station precinct, inside the passenger carriage, and platform) of the Seoul metropolitan subway. Among investigated areas, the levels of airborne bacteria and fungi in the workers' bedroom and station precincts were relatively high. No significant difference was found in the concentration of airborne bacteria and fungi between the underground and above ground activity areas of the subway. The genera identified in all subway activity areas with a 5% or greater detection rate were Staphylococcus, Micrococcus, Bacillus and Corynebacterium for airborne bacteria and Penicillium, Cladosporium, Chrysosporium, Aspergillus for airborne fungi. Staphylococcus and Micrococcus comprised over 50% of the total airborne bacteria and Penicillium and Cladosporium comprised over 60% of the total airborne fungi, thus these four genera are the predominant genera in the subway station.

Distribution characteristics of airborne bacteria and fungi in the general hospitals of Korea.

The objective of this study is to provide fundamental data related to size-based characteristics of bioaerosol distributed in the general hospital. Measurement sites are main lobby, ICU, surgical ward and biomedical laboratory and total five times were sampled with six-stage cascade impactor. Mean concentrations of airborne bacteria and fungi were the highest in main lobby as followed by an order of surgical ward, ICU and biomedical laboratory. The predominant genera of airborne bacteria identified in the general hospital were Staphylococcus spp. (50%), Micrococcus spp. (15-20%), Corynebacterium spp. (5-20%), and Bacillus spp. (5-15%). On the other hand, the predominant genera of airborne fungi identified in the general hospital were Cladosporium spp. (30%), Penicillium spp. (20-25%), Aspergillus spp. (15-20%), and Alternaria spp. (10-20%). The detection rate was generally highest on stage 5 (1.1-2.1 microm) for airborne bacteria and on stage 1 (>7.0 microm) for airborne fungi.

Effects of ethanol and phenobarbital on hemoglobin adducts formation in rats exposed to benzidine and Direct Black 38.

The objective of this study was to evaluate the effects of pretreatment of ethanol (EtOH) and phenobarbital (PB), which are known to affect the metabolism of xenobiotics, in the formation of hemoglobin adducts in rats administered with benzidine (BZ) and Direct Black 38 (DB38). The experimental rats were divided into BZ and DB38 groups; each group was subdivided into control, EtOH, and PB groups. Blood samples were separated into hemoglobin and plasma immediately after obtaining and basic hydrolysis was done to convert the adducts into aromatic amines. Hydrolyzed BZ, monoacetylbenzidine (MABZ), and 4-aminobiphenyl (4ABP) were separated by reversed-phase liquid chromatography without derivatization. Then, quantitative analyses were performed using a high performance liquid chromatograph equipped with an electrochemical detector. The amount of metabolites was expressed in the hemoglobin binding index (HBI). As a result, the formations of hemoglobin in BZ-, MABZ-, and 4ABP-HBI of BZ-EtOH and BZ-PB groups were increased compared with those of BZ-control group. In DB38 group, all of HBIs except for BZ-HBI were increased more than those of DB38-control group regardless of the pretreatment. These results are attributable to the fact that EtOH and PB induced N-hydroxylation is related to the formation of hemoglobin adducts. They indicate that EtOH not only increases the adduct formation by inducing N-hydroxylation but also induces N-acetylation. PB induced N-hydroxylation and increased the adduct formation in BZ group, but decreased the adduct formation in DB38 group due to decreasing azo reduction. These results suggest that the effects of EtOH or PB should be considered in biochemical monitoring of BZ and DB38 for the assessment of intermittent exposure to BZ and DB38.

Distribution characteristics of airborne bacteria and fungi in the feedstuff-manufacturing factories.

The objective of the study is to investigate the distribution patterns of airborne bacteria and fungi in three feedstuff-manufacturing factories in Korea. The geometric mean (+/-GSD) levels of airborne bacteria and fungi in the feedstuff-manufacturing factory were 113(+/-18)cfu/m(3) and 89(+/-5)cfu/m(3) for pelleting process and 198(+/-5)cfu/m(3) and 124(+/-12)cfu/m(3) for powdering process, respectively. The percentage of respirable (0.65-4.7 microm) and total (>4.7 microm) concentration of airborne bacteria and fungi in the feedstuff-manufacturing factory ranged from 60% to 90% and were higher in pelleting process than powdering process. The ratio of indoor concentration and concentration in ventilation air from outside for airborne bacteria and fungi exceeded 1.0 regardless of types of feedstuff manufacture process. The predominant airborne microorganisms in the feedstuff-manufacturing factory were Staphylococcus spp., Micrococcus spp., Corynebacterium spp. and Bacillus spp. for airborne bacteria and Cladosporium spp., Penicillium spp. and Aspergillus spp. for airborne fungi, respectively. Based on the result of the study, there would be an association between relative humidity among environmental factors and airborne microorganism's bioactivity.

Oxidative stress and antioxidant defenses in asthmatic murine model exposed to printer emissions and environmental tobacco smoke.

Exposure to particulate emissions from printer and cigarette smoke affects the structure and function of mitochondria, which may account for the pathogenesis of respiratory diseases. The addition of charge for the pollutant aerosols may increase the toxicity by their deposition in the lower respiratory tract. The mitochondrial damage in the lung of asthmatic mice was assessed by examining the levels of reactive oxygen species (ROS), lipid peroxides, reduced glutathione, and the activities of isocitrate dehydrogenase, alpha-ketoglutarate dehydrogenase, succinate dehydrogenase, malate dehydrogenase, complexes I to IV, and cytochrome c. The oxidative phosphorylation (levels of adenosine triphosphatase) was evaluated for the assessment of mitochondrial functional capacity. We found highly significant elevated levels of ROS, lipid peroxides, and decreased levels of mitochondrial enzymes in the mice exposed to environmental tobacco smoke and printer emissions + environmental tobacco smoke (ETS). However, mice exposed to printer emissions alone exhibited slight significant variations in the parameters studied. From the results, we conclude that printer emissions exert a synergistic effect in the presence of ETS and induce intense damage to the lung mitochondria by disrupting the structural and functional integrity of the mitochondrial membrane.