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1.
Stem Cells Dev ; 22(5): 735-49, 2013 Mar 01.
Artículo en Inglés | MEDLINE | ID: mdl-23025754

RESUMEN

Approximately 12.5% of all 9,920 extant bird species in the world are threatened with extinction, and yet conservation efforts through natural breeding of captive species continue to encounter difficulties. However, sperm cryopreservation and artificial insemination offer potential benefits over natural breeding, but their applicability is still limited in nondomestic species. In this study, we aimed to exploit the potential of germ cell xenotransplantation as an alternative tool for preserving germplasm of endangered birds. The study was designed to investigate whether transfer of either spermatogonia-enriched cell fraction (SEF) or crude testicular cell fraction (CTF) from adult Japanese quails (as a model for wild species) would result in recolonization of gamma-irradiated gonads of adult recipient chickens. One month after transplantation, 75% of recipients injected with SEF and 25% of recipients injected with CTF resumed spermatogenesis. However, it took more than 3 months for 33% of the negative controls to resume marginal production of sperm. Some SEF recipients produced more spermatozoa bearing head morphology compared with donor controls. DNA analysis using quail-specific primers did not detect donor's DNA in these recipients' semen. However, 6 months after xenotransplantation, presence of quail germ cells was demonstrated by polymerase chain reaction and by immunohistochemistry in 1 rooster injected with SEF. These findings indicate that spermatogonia from adult quails were capable of colonizing immunocompetent testis of adult chickens but failed to produce sufficient sperm. Despite this limitation, the present approach represents a potential conservation tool that may be used to rescue germ cells of endangered adult male birds.


Asunto(s)
Pollos , Coturnix , Espermatogénesis , Espermatogonias/trasplante , Espermatozoides/trasplante , Testículo/citología , Trasplante Heterólogo/veterinaria , Animales , Cruzamiento , Pollos/fisiología , Coturnix/fisiología , Especies en Peligro de Extinción , Femenino , Inseminación Artificial , Masculino , Espermatozoides/fisiología
2.
Proc Natl Acad Sci U S A ; 105(6): 2017-21, 2008 Feb 12.
Artículo en Inglés | MEDLINE | ID: mdl-18250302

RESUMEN

Although leptin is known for its regulation of food intake, it has many emerging roles in immune function. To better define the role of leptin in hematopoietic processes, a leptin-deficient obese mouse (ob/ob) and C57BL/6 lean wild-type controls were compared. Despite their large size and consumption of substantial amounts of nutrients, the ob/ob mice had only 60% as many nucleated cells in their marrow as controls. The greatest impact of leptin deficiency was on the B cell compartment that had 70% fewer cells, reducing the absolute number of pre-B and immature B cells to 21% and 12% of normal, respectively, and indicating a significant reduction in lymphopoiesis in ob/ob mice. Whereas the proportion of myeloids remained nearly normal in the obese mice, they also exhibited a reduction of 40% and 25%, respectively, in absolute numbers of granulocytes and monocytes. Seven days of provision of recombinant leptin promoted substantial lymphopoiesis, increasing the numbers of B cells in the marrow of the obese mice twofold, while doubling and tripling, respectively, the numbers of pre-B and immature B cells. Twelve days of supplementation brought these subpopulations to near-normal proportions. Leptin treatment also facilitated myelopoiesis such that the marrow of the obese mice contained normal numbers of monocytes and granulocytes after 7 days. Taken together, the data support an important role for leptin in sustaining lymphopoiesis and myelopoiesis.


Asunto(s)
Hematopoyesis/fisiología , Leptina/fisiología , Obesidad/fisiopatología , Animales , Peso Corporal/fisiología , Conducta Alimentaria/fisiología , Leptina/deficiencia , Ratones , Ratones Endogámicos C57BL
3.
Proc Natl Acad Sci U S A ; 105(6): 2028-33, 2008 Feb 12.
Artículo en Inglés | MEDLINE | ID: mdl-18250324

RESUMEN

Natural glucocorticoids (Gc) produced during stress have profound effects on the immune system. It is well known that Gc induce apoptosis in precursor T and B cells, markedly altering lymphopoiesis. However, it has been noted that marrow myeloid cells expanded both in proportion and absolute numbers in the mouse after Gc exposure. Mice were implanted with a corticosterone (CS) tablet that increased serum Gc and caused atrophied thymuses, both classic signs of activation of the stress axis. Blood neutrophil counts were elevated (4.8x), whereas lymphocyte counts declined. Flow cytometric analysis of the marrow revealed that the phenotypic distribution of the various major classes of cells was shifted by Gc exposure. As expected, marrow lymphocyte numbers declined >40% after 3 days of exposure to Gc. Conversely, in the myeloid compartment, both monocytes and granulocytes increased in number by >40%. Further, all granulocyte developmental stages showed large increases in both total number and percentage of cells. To investigate the functional capacity of mature granulocytes from Gc-treated mice, an improved granulocyte isolation method was developed. Gc exposure had little effect on the ability of granulocytes to produce superoxide or undergo chemotaxis or phagocytose bacteria. These results indicate that Gc treatment shifts bone marrow composition and provides evidence that granulocytes and their progenitors are selectively preserved under stressful conditions without losing function.


Asunto(s)
Células de la Médula Ósea/citología , Glucocorticoides/fisiología , Granulocitos/citología , Animales , Células de la Médula Ósea/inmunología , Separación Celular , Femenino , Citometría de Flujo , Granulocitos/inmunología , Ratones , Fagocitosis , Timo/patología
4.
J Am Coll Nutr ; 24(6): 494-502, 2005 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-16373946

RESUMEN

OBJECTIVE: The purpose of this study was to determine the impact of chronic zinc deficiency (ChrZD) on T and B cell lymphopoiesis, myelopoiesis and erythropoiesis in mice. METHODS: Young adult mice were fed a zinc adequate (ZA) or ChrZD synthetic diet for 34, 45, and 50 days. The cellular composition of the thymus and marrow were determined to assess the impact of ChrZD on lymphopoietic and hematopoietic processes using flow cytometry. Body weights, serum zinc and corticosterone (Cs) were monitored. RESULTS: For ChrZD mice growth was reduced 10% and serum zinc declined 15% by d 34 compared to ZA mice. By d 50 a 25% decrease in growth and 70% depression in serum zinc was noted though there was never any significant reduction in diet intake. Corticosterone rose 2.5 fold by d 34 and remained elevated in ChrZD mice indicating induction of the stress axis. At d 34 the thymus of ChrZD mice was normal but by d 50 there was a 50% cell loss and a 10% reduction in the proportion of Pre-T cells. Most importantly there was a 60% increase in Pre-T cells undergoing apoptosis in ChrZD mice. Pro-T, T helper, and T cytolytic populations were more resistant to ChrZD. Bone marrow cellularity and granulocyte, monocyte, and lymphocyte compartments remained unchanged in ChrZD mice. However, the erythroid compartment was reduced by 35% at d 50. CONCLUSIONS: The thymus was the most sensitive primary tissue to ChrZD. By d 50 it had atrophied by 36% with significant loss of Pre-T cells via apoptosis such that T-cell lymphopoiesis was disrupted. Significant reductions were also noted in the erythropoietic population by d 50. Conversely the marrow maintained myelopoiesis and B cell lymphopoiesis for the 50 d period indicating greater ability to survive a chronic zinc deficiency and exposure to Cs. The anemia and T cell lymphopenia associated with ChrZD in both rodents and humans may result from a greater sensitivity of their precursor cells to zinc deficiency and elevated Cs.


Asunto(s)
Linfocitos B/metabolismo , Eritropoyesis/fisiología , Linfopoyesis/fisiología , Mielopoyesis/fisiología , Linfocitos T/metabolismo , Zinc/deficiencia , Animales , Apoptosis , Linfocitos B/inmunología , Células de la Médula Ósea/inmunología , Células de la Médula Ósea/patología , Recuento de Células , Corticosterona/sangre , Eritropoyesis/efectos de los fármacos , Femenino , Citometría de Flujo , Inmunofenotipificación , Linfopoyesis/efectos de los fármacos , Ratones , Mielopoyesis/efectos de los fármacos , Tamaño de los Órganos , Distribución Aleatoria , Linfocitos T/inmunología , Timo/citología , Timo/inmunología , Timo/patología , Zinc/farmacología
5.
Annu Rev Nutr ; 24: 277-98, 2004.
Artículo en Inglés | MEDLINE | ID: mdl-15189122

RESUMEN

Thymic atrophy, lymphopenia, and compromised cell- and antibody-mediated responses that cause increased rates of infections of longer duration are the immunological hallmarks of zinc deficiency (ZD) in humans and higher animals. As the deficiency advances, a reprogramming of the immune system occurs, beginning with the activation of the stress axis and chronic production of glucocorticoids that accelerate apoptosis among pre-B and -T cells. This reduces lymphopoiesis and causes atrophy of the thymus. In contrast, myelopoiesis is preserved, thereby providing protection for the first line of immune defense or innate immunity. Changes in gene expression for cytokines, DNA repair enzymes, zinc transporters, signaling molecules, etc., suggest that cells of the immune system are attempting to adapt to the stress of suboptimal zinc. Better understanding of the molecular and cellular changes made in response to inadequate zinc should lead to the development of immunotherapeutic interventions.


Asunto(s)
Apoptosis/fisiología , Sistema Inmunológico/efectos de los fármacos , Zinc/deficiencia , Zinc/farmacología , Formación de Anticuerpos/efectos de los fármacos , Eritropoyesis , Regulación de la Expresión Génica , Glucocorticoides/biosíntesis , Humanos , Inmunidad Celular/efectos de los fármacos , Mielopoyesis
6.
Toxicol Appl Pharmacol ; 187(2): 69-79, 2003 Mar 01.
Artículo en Inglés | MEDLINE | ID: mdl-12649039

RESUMEN

Lipopolysaccharide (LPS) and vomitoxin (VT) synergistically induce glucocorticoid- mediated apoptotic cell death in lymphoid tissues of the mouse. Based on the known effects of glucocorticoids, it was hypothesized that the combined exposure to LPS and VT targets immature lymphocyte populations. To test this hypothesis, we quantified the effects of VT and LPS on apoptosis induction in T lymphocyte subsets in thymus and B lymphocyte subsets in Peyer's patches and bone marrow. Flow cytometry revealed that a single dose of LPS (0.1 mg/kg body wt ip) together with VT (12.5 mg/kg body wt po) promoted apoptosis of immature (CD4(-)CD8(-), CD4(+)CD8(+)) and mature (CD4(-)CD8(+)) thymocytes at 12 h with a subsequent reduction of these populations being detectable at 24 h. RU 486, a glucocorticoid receptor antagonist, significantly abrogated apoptosis in CD4(-)CD8(-), CD4(+)CD8(+), and CD4(-)CD8(+) subsets and also prevented loss in cell numbers. In Peyer's patches, mature-B lymphocytes (B220(+)IgM(-)IgD(+)) underwent apoptosis and, in bone marrow, pro/pre-B lymphocytes (B220(+)IgM(-)IgD(-)) and mature-B lymphocytes (B220(+)IgM(-)IgD(+)) underwent apoptosis at 12 h after toxin co- exposure. RU 486 blocked LPS + VT-induced apoptosis of the aforementioned subsets in Peyer patches and bone marrow at 12 h. Taken together, these data suggest that LPS can interact with VT in mice to induce the glucocorticoid-driven apoptotic loss of immature thymocytes and cytotoxic T lymphocytes in thymus, mature-B lymphocytes in Peyer's patch, and pro/pre-B lymphocytes and mature-B lymphocytes in bone marrow in mice.


Asunto(s)
Apoptosis/efectos de los fármacos , Linfocitos B/efectos de los fármacos , Lipopolisacáridos/toxicidad , Linfocitos T Citotóxicos/efectos de los fármacos , Tricotecenos/toxicidad , Animales , Apoptosis/inmunología , Linfocitos B/citología , Linfocitos B/metabolismo , Médula Ósea/inmunología , Médula Ósea/metabolismo , Relación CD4-CD8 , Cruzamientos Genéticos , Citometría de Flujo , Antagonistas de Hormonas/farmacología , Inmunoglobulina M/inmunología , Inmunoglobulina M/metabolismo , Lipopolisacáridos/inmunología , Subgrupos Linfocitarios/efectos de los fármacos , Subgrupos Linfocitarios/inmunología , Subgrupos Linfocitarios/metabolismo , Masculino , Ratones , Ratones Endogámicos C3H , Ratones Endogámicos C57BL , Mifepristona/farmacología , Ganglios Linfáticos Agregados/citología , Ganglios Linfáticos Agregados/inmunología , Ganglios Linfáticos Agregados/metabolismo , Linfocitos T Citotóxicos/citología , Linfocitos T Citotóxicos/metabolismo , Timo/citología , Timo/inmunología , Timo/metabolismo , Tricotecenos/inmunología
7.
J Nutr ; 132(11): 3301-7, 2002 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-12421843

RESUMEN

Suboptimal nutriture causes leukopenia, but whether this is related to a modification in hematopoiesis is unknown. A 34-d period of zinc deficiency was used to obtain moderate and severely zinc-deficient (ZD) young adult mice whose bone marrow was evaluated for alterations in hematopoiesis, myelopoiesis and lymphopoiesis by flow cytometry. Expressions of CD31 (PECAM-1) and Ly-6C were used to identify changes in marrow population composition. Identity of marrow cells was confirmed with TER119, CD45R, Ly-6G and CD11b. Cells of the erythroid lineage declined as much as 60% depending on the degree of zinc deficiency, providing new insight into the early observations of clinicians that anemia accompanied ZD in humans. The lymphoid compartment also declined 50-70% with preferential losses among pre-B cells, an underlying cause of the lymphopenia that is a part of ZD, in which loss of pre-B cells was identified by CD43,CD45R, and immunoglobulin M. Conversely, cells of the myeloid lineage increased substantially in the marrow, both in proportion and absolute numbers in all ZD mice. Granulocytic cells increased 40-60%, whereas monocytic cells nearly doubled in ZD mice. These data suggest that there are important adaptations in hematopoietic functions as zinc becomes limiting. In the immune system, the precursors of phagocytic cells, which provide innate immunity, are protected, whereas precursors of lymphocytes, which provide adaptive immunity, are down-regulated. These findings illuminate the unique response of the marrow to a nutritional stress.


Asunto(s)
Hematopoyesis , Mielopoyesis , Zinc/deficiencia , Animales , Linfocitos B/inmunología , Biotinilación , Células de la Médula Ósea/inmunología , Células de la Médula Ósea/patología , Recuento de Células , Eritropoyesis , Citometría de Flujo , Granulocitos/inmunología , Inmunoglobulina D/análisis , Inmunoglobulina M/análisis , Inmunofenotipificación , Linfocitos/inmunología , Ratones , Ratones Endogámicos A
8.
Toxicol Appl Pharmacol ; 180(1): 43-55, 2002 Apr 01.
Artículo en Inglés | MEDLINE | ID: mdl-11922776

RESUMEN

Exposure to bacterial endotoxin (lipopolysaccharide, LPS) is quite common and may increase human susceptibility to chemical-induced tissue injury. The purpose of this study was to identify mechanisms by which LPS potentiates lymphoid tissue depletion in B6C3F1 mice exposed to the common food-borne trichothecene mycotoxin, vomitoxin (VT). As demonstrated by DNA fragmentation and flow cytometric analysis, apoptosis in thymus, Peyer's patches, and bone marrow was marked in mice 12 h after administering Escherichia coli LPS (0.1 mg/kg body wt ip) concurrently with VT (12.5 mg/kg body wt po), whereas apoptosis in control mice or mice treated with either toxin alone was minimal. Based on observed increases in tumor necrosis factor-alpha (TNF-alpha) and interleukin (IL)-6 serum concentrations following LPS and VT cotreatment, the roles of these cytokines in apoptosis potentiation were assessed. Injection with rolipram, an inhibitor of TNF-alpha expression, or use of IL-6 knockout mice was ineffective at impairing thymic apoptosis induction by the toxin cotreatment, suggesting that these cytokines did not mediate LPS potentiation. Toxin cotreatment increased splenic cyclooxygenase-2 mRNA expression, suggesting possible involvement of prostaglandins in apoptosis. However, indomethacin, a broad spectrum inhibitor of cyclooxygenases, failed to block thymus apoptosis. Toxin cotreatment increased serum corticosterone and, furthermore, RU 486, a glucocorticoid receptor antagonist, significantly abrogated apoptosis in thymus, Peyer's patches, and bone marrow following LPS + VT exposure. The results presented herein and the known capacity of glucocorticoids to cause apoptosis indicate that hypothalamic-pituitary-adrenal axis plays a key role in LPS potentiation of trichothecene-induced lymphocyte apoptosis.


Asunto(s)
Apoptosis/efectos de los fármacos , Corticosterona/biosíntesis , Lipopolisacáridos/farmacología , Linfocitos/efectos de los fármacos , Tricotecenos/toxicidad , Animales , Corticosterona/sangre , Ciclooxigenasa 2 , Fragmentación del ADN/efectos de los fármacos , Sinergismo Farmacológico , Escherichia coli/inmunología , Citometría de Flujo , Indometacina/farmacología , Interleucina-6/sangre , Interleucina-6/deficiencia , Interleucina-6/genética , Isoenzimas/biosíntesis , Isoenzimas/genética , Linfocitos/patología , Tejido Linfoide/efectos de los fármacos , Tejido Linfoide/patología , Masculino , Ratones , Ratones Endogámicos C3H , Ratones Endogámicos C57BL , Ratones Noqueados , Mifepristona/farmacología , Prostaglandina-Endoperóxido Sintasas/biosíntesis , Prostaglandina-Endoperóxido Sintasas/genética , Factor de Necrosis Tumoral alfa/análisis , Regulación hacia Arriba
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