Anti-Inflammatory Effect of Technologically Processed Antibodies to the Molecules of the Major Histocompatibility Complex in a Model of Acute Inflammation In Vivo.

The anti-inflammatory effect of technologically processed antibodies (TPA) to immune targets (MHC I and MHC II) was assessed in the carrageenan-induced rat paw edema model. The parameters "increase in edema" and "suppression of edema" significantly decreased (p<0.05) against the background of treatment with TPA and the reference drug indomethacin compared to the placebo group. The tested TPA produced an anti-inflammatory effect.

Prevention of the Genotoxic Effects of Doxorubicin with Anthocyanin-Containing Complex from Sorbus aucuparia L. Fruit.

We studied the effect of an anthocyanin-containing complex from the fruits of S. aucuparia L. on doxorubicin-induced genotoxicity in bone marrow cells of C57BL/6 mice. The complex reduced the genotoxic effect doxorubicin in metaphase plates of bone marrow cells in 24, 48 h, and 10 days after the administration of the cytostatic. The mean number of single fragments and the fraction of cells with gaps and aberrant metaphases also decreased.

Hypolypidemic Activity of L-Rhamnopyranosyl-6-O-Methyl-D-Galacturonan, a Polysaccharide Isolated from Birch Leaves (Betula pendula L.).

In male Syrian hamsters fed a synthetic high-fat diet enriched with cholesterol (0.3%), administration of a polysaccharide from birch leaves L-rhamnopyranosyl-6-O-methyl-D-galacturonan (3 g/100 g of diet) resulted in a decrease in total cholesterol levels, mainly due to the LDL fraction, triglycerides, and bile acids in blood serum; the content of triglycerides and cholesterol in the liver also decreased, while excretion of bile acids with feces increased. Thus, the lipid-lowering effect of L-rhamnopyranosyl-6-O-methyl-D-galacturonan is related to its ability to bind bile acids in the intestine and interrupt their enterohepatic circulation.

Erythropoiesis-Stimulating Properties of Anthocyanin-Containing Complex from Sorbus aucuparia L. in Cytostatic Anemic Syndrome in Mice with Lewis Lung Carcinoma.

The results of studying the effect of the anthocyanin-containing complex from Sorbus aucuparia L. on the main indicators of erythropoiesis in the blood and bone marrow of mice with Lewis lung carcinoma against the background of doxorubicin administration were presented. It was shown that administration of the anthocyanin-containing complex from S. aucuparia L. to animals with the tumor prevented the development of anemic syndrome by promoting regeneration of the erythropoiesis after its depletion caused by single administration of a cytostatic agent.

Antinociceptive Effect of Ethowurtzine, a New Compound from the Class of Hexaazaizowurzitane.

Analysis of specific pharmacological activity evaluated high antinociceptive efficacy of the first synthesized compound 10-di(ethoxyacetyl)-2,6,8,12-tetraacetyl-2,4,6,8,10,12-hexaazatetracyclo[5,5,0,03,11,05,9]dodecane (ethowurtzine) in models of somatogenic pain of different genesis (thermal, visceral pain, mechanical compression of paw).The new molecule from the class of hexaazaisowurtzitane effectively blocks nociceptive reactions at the supraspinal and peripheral levels of pain sensitivity organization. The effect of ethowurtzine was comparable or exceeded the effect of tramadol. The obtained results prove the possibility of creating new pharmacologically active molecules based on the high-energy substance hexaazaisowurtzitane.

Erythropoesis-Stimulating Properties of Anthocyanin-Containing Complexes in Cytostatic Anemic Syndrome.

We studied the effect of an anthocyanin-containing complex from Sorbus aucuparia L. on the main indicators of erythropoiesis in the blood and bone marrow of mice against the background of doxorubicin treatment. It was shown that administration of an anthocyanincontaining complex from S. aucuparia L. prevented the development of anemic syndrome by stimulating regeneration of the erythroid lineage of hematopoiesis after its devastation by single administration of the cytostatic.

Analgesic Activity of Hexaazaisowurtzitane Derivatives.

Pronounced analgesic activity of the innovative compound 4-(3,4-dibromthiophencarbonyl)-2,6,8,12-tetraacethyl-2,4,6,8,10,12hexaazatetracyclo[5,5,0,03, 11,05, 9] dodecane (thiowurtzine) was observed in the thermal nociceptive hot plate test and in the acute visceral and somatic deep pain model (acetic acid writhing test). In these experimental models, naloxone-sensitive thiowurtzine-induced analgesia was revealed. The absence of tropism to peripheral opioid receptors in the acetic acid writhing test was demonstrated using naloxone methiodide. Course administration of low-toxic thiowurtzine in effective doses was not associated with ulcerogenic damage to the gastric mucosa in experimental animals.

[Rationale for caries prevention in young adults based on caries resistance rates]. / Obosnovanie metodov profilaktiki kariesa u lits molodogo vozrasta na osnove pokazatelei kariesrezistentnosti.

The aim of the study was to evaluate the effectiveness of caries prevention in young adults with different caries resistance rates. Sixty-seven patients aged 18-20 with low (n=32) and high (n=35) dmft were enrolled in the study. Caries prevention efficacy was assessed by laboratory tests: enamel resistance test (ERT), clinical enamel remineralization speed test (CERST), estimation of calcium and inorganic phosphorus in oral fluid. Patients with high caries activity in addition to conventional prevention program received prophylaxis procedure with Clinpro White Varnish (3M ESPE). ERT and CERST were significantly higher in high dmft (>13) subgroup. Statistically significant difference in ERT and CERST was observed between subgroups at 3 and 6 months evaluation after prevention program.

[Acid-base disturbances during liver transplantation].

UNLABELLED: Objective of the study is to assess the contribution of different factors in the development of acid-base disturbances at the stages of liver transplantation. MATHERIALS AND METHODS: The analysis of right lobe relative liver transplantation was held in 86 recipients. 22 patients corresponded to ASA III (group 1), ASA IV - 50 patients (group 2), and ASA V - 14 patients (group 3). Blood samples were studied by pH, SB, lactate, pCO2 at the stages: up to the beginning, before v. cava inferior cross-clamping, before blood flow launch, on the 1st minute after blood flow launch, 5 min after blood flow launch. 1 hour after the blood flow launch, 2 hours after blood flow launch, at the end of operations. Cardiac index and oxygen delivery were also estimated RESULTS: The preliverless stage was characterized by a decrease in pH, SB. BE and increased lactate, oxygen delivery slightly reduced due to the reduction of oxygen blood capacity, cardiac index remained within the normal range. During liverless period, the growth rate of lactate was different in all three groups, DO2 was below the norm, CI - on the lower bound of the norm. Blood flow launch was accompanied by a peak values of pH, SBC, BE, lactate and increased pCO2. CONCLUSIONS: The main factor in the development of metabolic acidosis during preliverless stage is lactate growth as a consequence of decreased hepatic lactate clearance and blood loss. During liverless period the most significant impact contributes to reduced cardiac output, which, together with reduced oxygen blood capacity leads to a decrease in tissue DO2. Increased production of lactate, together with a decrease in its clearance due to liver shutdown from the bloodstream leads to higher rates of lactate growth in this period. When starting the blood flow in addition to the release of acidic substances, growth of endogenous CO2 leads to the peak pH values.

[To the issue of postreperfusion syndrome predictors in orthotopic liver transplantation (OLT)].

UNLABELLED: The aim of the study is revelation of postperfusion syndrome (pPS) predictors in orthotopic liver transplantation (OLT). MATERIALS AND METHODS: Was conducted a retrospective analysis of anesthesia maintainance protocols during orthotopic liver transplantation in 261 patients aged from 6 months to 60 years. Investigated the effect of various factors on the development of PPS by the application of methods of non-parametric statistics. RESULTS: Significantly more frequent development of the PPS is noted in the age group from 3 to 18 years (up to 30% of patients). In recipients older than 18 years the frequency of the development of the PPS does not depend on age, with an average of 14%. The development of the PPS does not depend on the recipient sex, the nature of the pathology which served as an indication to the OTP, the initial severity of the state, type of OTP (living related donor or cadaveric transplantation, primary or re-transplantation), the transplant warm ischemia duration, use, or the lack of venous-venous bypass, metabolic status of the patient. The obtained results do not contradict to the data of foreign publications. Among parameters available for screening, predictor of PPS was not detected.

[Clinical laboratory substantiation of immunocorrection use in comprehensive treatment of chronic catarrhal gingivitis].

The role of immune system in development of chronic catarrhal gingivitis in 60 primary patients was defined and substantition of immunocorrection with Licopid in combined treatment was done.

[Causes of lethal outcomes in presenile and senile patients treated in pulmonological hospital]. / Prichiny letal'nykh iskhodov u lits pozhilogo i starcheskogo vozrasta v usloviiakh pul'monologicheskogo statsionara.

Causes of lethal outcomes were analysed for 46 patients (mean age 76.76 years, 39 males, or 84.78%, and 7 females, or 15.22%) why died of respiratory diseases when treated in War Veterans Hospital of St-Petersburg. Necropsies registered the following causes of death: chronic obstructive pulmonary disease (COPD) (32 deaths, 69.57%), cardiovascular diseases (17 deaths, 36.96%), cancer (14 deaths, 30.44%). Lethal outcomes were most frequent at the age of 71 to 80 years (52.17%). COPD was present in 43 of 46 of the deceased (93.48%). The most frequent direct causes of lethal outcomes in elderly patients with COPD were isolated pulmonary-cardiac insufficiency and combination of this insufficiency with pneumonia which is as an aggravating factor and determines the outcome of the basic disease.

Immunosorbent for IgG apheresis: an in vitro study.

We obtained anti-IgG Sepharose-an immunosorbent with sheep monospecific polyclonal antibodies against human IgG-for extracorporeal removal of human IgG. Appropriate conditions for effective usage of the immunosorbent were chosen. Binding capacity and selectivity of anti-IgG Sepharose were determined and compared with the corresponding properties of other sorbents for IgG apheresis; these properties appeared to be acceptable for immunoapheresis procedures. Methods for therapeutic application of anti-IgG Sepharose is expected to be effective when used in immunoapheresis procedures for treatment of autoimmune diseases and for preparation of patients for organ transplantation.