[Concentrations of prolactin, LH, estradiol-17 beta and progesterone in swine after biotechnical stimulation with PMSG, HCG and suidor]. / Konzentrationen von Prolaktin, LH, Estradiol-17 beta und Progesteron beim Schwein nach biotechnischer Stimulation mit PMSG, HCG und Suidor.

Concentrations of estradiol-17 beta, progesterone, luteinising hormone (LH), and prolactin were recorded from 9 gilts, following cycle blocking by means of Suisynchron(R)-Prämix and application of PMSG, HCG, and Suidor(R). A radio-immuno-assay which for its quality criteria enabled safe determination of the hormone in peripheral blood had been worked out specifically for prolactin appraisal. HCG application led to blockage of pre-ovulatory LH release in 7 of 9 animals. Possible causes are discussed. Prolactin concentrations during oestrus were differentiated and, for example, were characterised by strong oscillatory variations. With the experimental arrangement used, the boar pheromone Suidor(R) was not found to have any impact upon hormone profiles.

[The hormone profile in the blood plasma of heifers after cycle synchronization with prostaglandin F2alpha]. / Untersuchungen zum Hormonprofil im Blutplasma von Färsen nach Zyklussynchronisation mit Prostaglandin F2alpha.

Highly sensitive radio-immuno assay (RIA) was used for 21 days to study the curves of several steroid hormones, progesterone (P), oestradiol 17 beta (E2), and testosterone (T), as well as of luteinizing hormone (LH) in peripheral blood of six heifers to which oestrus had been induced by prostaglandin F2 alpha. The validity of the authors' RIA for P, E2, and T determination in blood plasma was positively verified by two reliability criteria, correctness and accuracy, as well as by comparative determinations, using reference methods. Only four of the six heifers returned to oestrus, within 21 days from first oestrus induction. A typical cycle-related curve of the P concentration in peripheral blood with peak values between the 13th and 17th days of cycle (15-26 nmol/l) was recorded from four animals. The peak values of pre-ovulatory E2 and LH were between 33.0 and 53.2 pmol/l or between 19.5 and 52.5 micrograms/l. Some of the T rises in peripheral blood were in parallel to E2 concentrations. All hormone curves are presented in detail and are discussed in relation to clinico-physiological findings.

[Steroid hormone levels in follicular fluid of swine with spontaneous estrus and ovulation synchronization]. / Steroidhormonspiegel in Follikelflüssigkeiten von spontanöstrischen und ovulationssynchronisierten Schweinen.

The steroid hormones oestradiol 17 beta, oestrone (E1), testosterone (T), and progesterone (P) were quantitatively determined in follicular fluids in the pre-oestric phase of pigs with spontaneous oestrus and synchronised ovulation. As a whole, mean steroid hormone concentrations exhibited characteristic curves in spite of a relatively wide scatter of individual values. Drastic decline in oestrogen concentration in pigs with spontaneous oestrus and synchronised ovulation was accompanied by rise of P levels, while T values stayed relatively constant. As compared to spontaneous oestrus, treatment for synchronised ovulation caused significantly higher increases in follicular T levels. Positive (P) or negative (E1, T) correlations were found to exist between follicle size and steroid hormone concentration. Conclusions are suggested for further studies.

[Experimental studies on the stimulation of prenatal development in swine by PMSG treatment in early pregnancy. 2. Analytic studies of hormones in young sows after PMSG treatment on the 11th day of pregnancy]. / Experimentelle Untersuchungen zur Stimulation der vorgeburtlichen Entwicklung beim Schwein durch PMSG-Behandlung in der frühen Trächtigkeit. 2. Mitteilung: Hormonanalytische Untersuchungen an Jungsauen nach PMSG-Behandlung am 11. Trächtigkeitstag.

Tens gilts received 400 I.U. of Pregmagon on the 11th day of pregnancy (day of KB2 = 1 day of pregnancy), while 8 sows were used as controls. Blood was sampled 4 times a day between the 11th and 25th day of pregnancy, with an indwelling catheter being used. Progesterone, oestradiol-17 beta, and luteinising hormone were radio-immunologically determined. The hormone profiles of pregnant sows were found to differ unambiguously from those of non-pregnant animals for both concentrations and frequencies. They were indicative of close correlations and time links of gonadotrophic and steroid hormones in early pregnancy and in oestrus. PMSG treatment in pregnancy resulted in increase in the oestradiol-17 beta concentration and attenuation in pulsatile luteinising hormone release at the time of implantation. Progesterone concentrations in peripheral blood were not affected by PMSG injection.