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BMC Genomics ; 25(1): 630, 2024 Jun 24.
Artículo en Inglés | MEDLINE | ID: mdl-38914936

RESUMEN

Deep Mutational Scanning (DMS) assays are powerful tools to study sequence-function relationships by measuring the effects of thousands of sequence variants on protein function. During a DMS experiment, several technical artefacts might distort non-linearly the functional score obtained, potentially biasing the interpretation of the results. We therefore tested several technical parameters in the deepPCA workflow, a DMS assay for protein-protein interactions, in order to identify technical sources of non-linearities. We found that parameters common to many DMS assays such as amount of transformed DNA, timepoint of harvest and library composition can cause non-linearities in the data. Designing experiments in a way to minimize these non-linear effects will improve the quantification and interpretation of mutation effects.


Asunto(s)
Mutación , Flujo de Trabajo , Proteínas/metabolismo , Proteínas/genética , Secuenciación de Nucleótidos de Alto Rendimiento , Mapeo de Interacción de Proteínas/métodos , Análisis Mutacional de ADN/métodos , Unión Proteica
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