RESUMEN
The distribution of iodine-125 labeled human alpha-fetoprotein in mice was studied after its intravenous injection. The maximal accumulation of alpha-fetoprotein in different tissues and organs of animals was observed mainly 5 hours after injection. Then the protein was gradually eliminated from the body. In the liver, intestine and blood of intact animals 125I-alpha-fetoprotein persists for at least three days. Accumulation of alpha-fetoprotein in various tissues and organs may determine the different biological effects of this protein. In the mice with transplanted lymphatic leukemia cells P388 the high level of alpha-fetoprotein accumulation was detected in the tumor tissue, reaching 6% of the injected amount per 1 g of tissue. This allows considering the radionuclide-labeled alpha-fetoprotein as a promising medical radionuclide marker for the radiological detection of malignant tumors.
Asunto(s)
Radioisótopos de Yodo/farmacocinética , Leucemia Linfoide/metabolismo , alfa-Fetoproteínas/farmacocinética , Animales , Femenino , Humanos , Inyecciones Intravenosas , Mucosa Intestinal/metabolismo , Hígado/metabolismo , Ratones , Ratones Endogámicos DBA , Cintigrafía , Distribución Tisular , Ensayos Antitumor por Modelo de XenoinjertoRESUMEN
We studied high-resolution (1)H nuclear magnetic resonance spectra of the serum and serum albumin from patients with the first episode of schizophrenia and healthy individuals. A relative increase in signal intensities of CH(2) protons in serum LDL and VLDL in schizophrenia was demonstrated. Higher intensities of CH(2) and CH(3) protons of non-esterified fatty acids were found in (1)H nuclear magnetic resonance spectra of serum albumin. These data attest to an essential role of changes in lipid metabolism and changed ligand load of albumin in schizophrenia.
Asunto(s)
Protones , Esquizofrenia/diagnóstico , Albúmina Sérica/química , Adolescente , Adulto , Femenino , Humanos , Metabolismo de los Lípidos , Lipoproteínas LDL/química , Lipoproteínas LDL/metabolismo , Lipoproteínas VLDL/química , Lipoproteínas VLDL/metabolismo , Masculino , Resonancia Magnética Nuclear Biomolecular , Conformación Proteica , Esquizofrenia/metabolismo , Albúmina Sérica/metabolismoRESUMEN
The conformation of calf skin collagen at the secondary structure level changed after 60Co-gamma-radiation with doses ranging from 50 to 1,000 Gy in aerated aqueous solutions and in the presence of N2 and N2O. Despiralization of collagen at a dose of 1,000 Gy reached 90% in N2O, 70% in N2, and 60% in the aerated solution. The radiochemical yield of the despiralized collagen molecules was 0.007 mol/100 eV owing to OH(.). The presence of radioprotective agents upon irradiation of collagen solutions diminished considerably the injury to its secondary structure.
