RESUMEN
Evaluation of tumor markers expression pattern which determines individual progression parameters is one of the major topics in molecular oncopathology research. This work presents research on expression analysis of several Ras-Ral associated signal transduction pathway proteins (Arf6, RalA and BIRC5) in accordance with clinical criteria in non small cell lung cancer patients. Using Western-blot analysis and RT-PCR Arf6, RalA and BIRC5 expression has been analyzed in parallel in 53 non small cell lung cancer samples of different origin. Arf6 protein expression was elevated in 55% non small cell lung cancer tumor samples in comparison with normal tissue. In the group of squamous cell lung cancer Arf6 expression elevation was observed more often. RalA protein expression was decreased in comparison to normal tissue samples in 64% of non small cell lung cancer regardless to morphological structure. Correlation between RalA protein expression decrease and absence of regional metastases was revealed for squamous cell lung cancer. BIRC5 protein expression in tumor samples versus corresponding normal tissue was 1.3 times more often elevated in the squamous cell lung cancer group (in 76% tumor samples). At the same time elevation of BIRC5 expression was fixed only in 63% of adenocarcinoma tumor samples. A statistically significant decrease (p = 0.0158) of RalA protein expression and increase (p = 0.0498) of Arf6 protein expression in comparison with normal tissue was found for T1-2N0M0 and T1-2N1-2M0 groups of squamous cell lung cancer correspondingly.
Asunto(s)
Factores de Ribosilacion-ADP/biosíntesis , Biomarcadores de Tumor/biosíntesis , Carcinoma de Pulmón de Células no Pequeñas/patología , Proteínas Inhibidoras de la Apoptosis/biosíntesis , Neoplasias Pulmonares/patología , Proteínas de Unión al GTP ral/biosíntesis , Factor 6 de Ribosilación del ADP , Factores de Ribosilacion-ADP/genética , Biomarcadores de Tumor/genética , Carcinoma de Pulmón de Células no Pequeñas/metabolismo , Expresión Génica/genética , Humanos , Proteínas Inhibidoras de la Apoptosis/genética , Neoplasias Pulmonares/metabolismo , Metástasis de la Neoplasia , Estadificación de Neoplasias , Survivin , Proteínas de Unión al GTP ral/genéticaRESUMEN
We studied the effect of low-fluence photodynamic treatment with Photosence preparation on functional properties of macrophages and endothelium in vitro. It was shown that low-intensity photodynamic treatment did not affect viability of these cells. Exposure (0.25 J/cm(2)) of endothelial cells loaded with Photosence did not change the expression of adhesion molecules, but reduced adhesion of peripheral blood mononuclears to these cells. The same low-intensity exposure inhibited phagocytic activity of macrophages and reduced activity of matrix metalloproteinase-9 produced by them.