Effects of nordic walking training on gait and exercise tolerance in male ischemic heart disease patients.

This technique-focused observational study explores the impact of a 6-week Nordic Walking (NW) program on physiological and biomechanical aspects in ischemic heart disease (IHD) patients. Twelve male IHD patients (66.2 ± 5.2 years, 12.2 ± 7.5 years of disease duration) were evaluated pre- and post-training for (i) gait parameters, (ii) exercise tolerance using electrocardiographic (ECG) stress test, (iii) a 6-min walk test (6MWT). The NW training, adhering to IHD patient guidelines, involved a 100-m walk at a self-selected, preferred speed without sticks, with classic NW sticks and mechatronic sticks. A mechatronic measuring system, specifically engineered for measuring, diagnosing and monitoring the patient's gait, was integrated into mechatronic sticks. Post-training, significant enhancements were observed in ECG stress test duration, metabolic equivalency, and 6MWT distance, irrespective of the stick type. However, no significant changes were noted in spatiotemporal parameters concerning the measured side, stick utilisation, or type. The results suggest that NW training boosts exercise capacity and refines gait mechanics in male IHD patients. However, the improvement in exercise capacity was not linked to changes in gait mechanics from NW training but rather to the movement during NW gait. Hence, the key to enhancing exercise capacity in IHD patients is the movement during NW gait, not the quality of gait mechanics.

Neuronal Fractal Dynamics.

Synapse formation is a unique biological phenomenon. The molecular biological perspective of this phenomenon is different from the fractal geometrical one. However, these perspectives are not mutually exclusive and supplement each other. The cornerstone of the first one is a chain of biochemical reactions with the Markov property, that is, a deterministic, conditional, memoryless process ordered in time and in space, in which the consecutive stages are determined by the expression of some regulatory proteins. The coordination of molecular and cellular events leading to synapse formation occurs in fractal time space, that is, the space that is not only the arena of events but also actively influences those events. This time space emerges owing to coupling of time and space through nonlinear dynamics. The process of synapse formation possesses fractal dynamics with non-Gaussian distribution of probability and a reduced number of molecular Markov chains ready for transfer of biologically relevant information.

Technical and Tactical Actions of the World's Leading Male Table Tennis Players Between 1970 and 2021.

This research aimed to determine the quantitative and qualitative structure of winning systems of the world's leading male table tennis players between 1970 and 2021. The study used the Wu game analysis method, modified by the authors, which consists of observing the game from playback, identifying the winning actions of a given player, and sorting and counting the actions, depending on the accuracy of the observation. The project identified all World Championships and Olympic Games medallists, resulting in 244 men's matches being analyzed. Three time periods were considered based on the ball used, including the 38 mm celluloid ball, 40 mm celluloid ball, and 40 mm plastic ball. Differences in the level structure, depending on the observation period, were assessed using the chi-squared test of independence. The Pearson contingency coefficient was calculated, and multiple comparisons were made. The research showed that the use of combinations changed slightly with changes in ball size and material. The first three strokes were very important in all periods. However, the importance of serves as direct scoring strokes decreased. These findings may be related to changes in the size and material used for ball production. The most winning serves in the game of the top men were side-spin forehand serves, which were also used most often during 3rd-ball-attack winning combinations. The number of actions won directly with a return, and in the return-counterattack combination, accounted for, and still constitute, about 30%, with an increasing number of backhand flicks. The research also showed an increase in the use of backhand strokes compared to forehand strokes. These findings should be considered when creating basic goals in table tennis training plans.

The level of actual functional fitness of men from different living environments in Poland and associations with health - a cross-sectional study.

BACKGROUND: The study aimed to assess the differences in functional fitness between older men living in social welfare homes (SWH) and men living in society. METHODS: The study involved 474 men aged 60-84, including 134 men living in social welfare homes and 340 men living in community. The Senior Fitness Test was used to assess functional fitness. Body weight and height were measured. Moreover, data on age, education, taking up physical activity and place of residence were collected. RESULTS: A significantly lower level of functional fitness of SWH residents was demonstrated compared to men living in the society (p < 0.001). A significant percentage of seniors living in SWH did not reach the normal range for the Polish population. Also, the level of education, physical activity and health self-assessment differentiated the institutionalized men from those living in the community. CONCLUSIONS: The place of residence is a factor that differentiates seniors' functional fitness levels. The reduced fitness of social welfare home residents may also result from the low level of their daily physical activity, education and health. Introducing physical activity programs for elderly residents in social welfare homes seems reasonable. TRIAL REGISTRATION: ISRCTN platform as 18,225,729; December 2020.

Does lumbar spinal decompression or fusion surgery influence outcome parameters in patients with intrathecal morphine treatment for persistent spinal pain syndrome type 2 (PSPS-T2).

OBJECTIVES: Intrathecal morphine pump (ITMP) infusion therapy is efficient in managing chronic pain refractory to standard treatment. This study evaluates pain relief and improvement of quality of life in chronic pain patients after intrathecal morphine pump implantation for treatment of persistent pain after lumbar spinal fusion surgery and lumbar spinal decompression alone. METHODS: Forty three chronic pain patients that received an ITMP at our department between 2009 and 2019 were retrospectively analyzed divided into 2 cohorts (lumbar spinal fusion surgery and lumbar spinal decompression alone). Pain intensity was evaluated using the numeric rating scale (NRS), quality of life was assessed by EQ-5D-3L, mental health was assessed by Beck Depression Inventory (BDI-V), and Pain Catastrophizing Scale (PCS). Morphine dosage was assessed over time. Data was collected preoperatively, 6 and 24 months postoperatively. Statistical analysis was performed using Friedman's analysis of variance to evaluate the development of NRS, PCS, BDI and EQ-5D-3L over time and Mann-Whitney-U-test for the differences between these parameters in the different cohorts. A two-sided p-value <0.05 was considered statistically significant. RESULTS: Median age was 64 years (IQR25-75 56-71 years). NRS, EQ-5D-3L, BDI-V, and PCS showed a significant overall improvement after 6 and 24 months compared to baseline data (p<0.001). No statistically significant differences between patients with lumbar spinal fusion surgery and lumbar spinal decompression alone were seen. Furthermore, no statistically significant differences for age and gender were seen. The initially administered median morphine dosage was significantly higher in the fusion group (3.0 mg/day; IQR25-75 1.5-4.2 mg/day) compared to the decompression-alone group (1.5 mg/day; IQR25-75 1.0-2.6 mg/day); (p=0.027). CONCLUSIONS: This retrospective study showed that ITMP have a major long-term impact on pain relief, improve the quality of life, psychological distress, as well as pain catastrophizing in patients with chronic pain following lumbar spinal surgery independent of the previous surgical procedure. After ITMP implantation initial median morphine dosage seems to be significantly higher after spinal fusion compared to decompressive surgery alone.

Do Mechatronic Poles Change the Gait Technique of Nordic Walking in Patients with Ischemic Heart Disease?

The study aimed to compare the technique of normal gait with the Nordic walking (NW) gait with classical and mechatronic poles in patients with ischemic heart disease. It was assumed that equipping classical NW poles with sensors enabling biomechanical gait analysis would not cause a change in the gait pattern. The study involved 12 men suffering from ischemic heart disease (age: 66.2 ± 5.2 years, body height: 173.8 ± 6.74 cm; body mass: 87.3 ± 10.89 kg; disease duration: 12.2 ± 7.5 years). The MyoMOTION 3D inertial motion capture system (Noraxon Inc., Scottsdale, AZ, USA) was used to collect biomechanical variables of gait (spatiotemporal and kinematic parameters). The subject's task was to cover the 100 m distance with three types of gait-walking without poles (normal gait), walking with classical poles to NW, and walking with mechatronic poles from the so-called preferred velocity. Parameters were measured on the right and left sides of the body. The data were analyzed using two-way repeated measures analysis of variance with the between-subject factor "body side." Friedman's test was used when necessary. For most kinematic parameters, with the exception of knee flexion-extension (p = 0.474) and shoulder flexion-extension (p = 0.094), significant differences were found between normal and walking with poles for both the left and right side of the body and no differences due to the type of pole. Differences between the left and right movement ranges were identified only for the ankle inversion-eversion parameter (gait without poles p = 0.047; gait with classical poles p = 0.013). In the case of spatiotemporal parameters, a reduction in the cadence step value using mechatronic poles and the stance phase using classical poles compared to normal walking was observed. There was also an increase in the values for step length and step time regardless of the type of poles, stride length, and swing phase when using classical poles and stride time when using mechatronic poles. The differences between the right and left sides of the measurement occurred when walking with both types of poles for single support (gait with classical poles p = 0.003; gait with mechatronic poles p = 0.030), stance phase (gait with classical poles p = 0.028; gait with mechatronic poles p = 0.017) and swing phase (gait with classical poles p = 0.028; gait with mechatronic poles p = 0.017). Mechatronic poles can be used in the study of the biomechanics of gait in real-time with feedback on its regularity because no statistically significant differences were found between the NW gait with classical and mechatronic poles in the studied men with ischemic heart disease.

Significantly higher expression of high-mobility group AT hook protein 2 (HMGA2) in the border zone of glioblastoma.

BACKGROUND: High-mobility group AT-hook protein 2 (HMGA2) is a gene regulatory protein that is correlated with metastatic potential and poor prognosis. It has been shown that HMGA2 is overexpressed in various tumors such as lung cancer or pancreatic cancer. The invasive character and highly aggressive structure of glioblastoma let us to investigate HMGA2 expression in the border zone of the tumor more closely. We compared HMGA2 expression between glioblastoma and normal brain tissue. In addition, we analyzed and compared HMGA2 expression in the border and center zones of tumors. Correlation tests between HMGA expression and clinical parameters such as MGMT-status and survival were performed. METHODS: Samples from 23 patients with WHO grade 4 glioblastomas were analyzed for HMGA2 expression using quantitative real-time polymerase chain reaction (qPCR) and immunohistochemistry (IHC) and correlated with clinical parameters. The areas from the tumor center and border were analyzed separately. Two normal brain tissue specimens were used as the controls. RESULTS: Our results confirm that HMGA2 is higher expressed in glioblastoma compared to healthy brain tissue (qPCR, P=0.013; IHC, P=0.04). Moreover, immunohistochemistry revealed significantly higher HMGA2 expression in the border zone of the tumor than in the tumor center zone (P=0.012). Survival analysis revealed a tendency for shorter survival when HMGA2 was highly expressed in the border zone. CONCLUSIONS: The results reveal an overexpression of HMGA2 in the border zone of glioblastomas; thus, the expression cluster of HMGA2 seems to be heterogenous and thorough borough surgical resection of the vital and aggressive border cells might be important to inhibit the invasive character of the tumor.

Selected Spatiotemporal and Joint Angle Parameters in Normal Gait and Nordic Walking with Classical and Mechatronic Poles in Aspects of Sex Differences.

Introduction: The aim of this study was to compare selected spatiotemporal parameters and changes in the range of motion in the joints of lower and upper limbs during normal gait and during Nordic walking performed with classical and mechatronic poles of females and males. Methods: The study involved 19 physical education students (11 males and 8 females). The MyoMotion research motion analysis system was used to collect gait kinematic variables. The subject task was to cover a 100 m distance in a straight line with three types of gait: gait without poles, gait with classical poles, and gait with mechatronic poles at preferred velocity. Parameters were measured both on the right (RT) and on the left side (LT) of the body. The data was analyzed using two-way repeated measures ANOVA with the between-subject factor "sex." Friedman's test was used when necessary. Results: The most significant differences in spatiotemporal parameters between males and females were revealed in gait with the classical and mechatronic pole (stance phase LT and RT, load response LT and RT, single support LT and RT, preswing LT and RT, swing phase LT and RT, double stance LT and RT, and step length LT), the least in gait without a pole (stance phase RT, load response LT, single support LT, preswing RT, and swing phase RT); whereas, the most significant differences in kinematic parameters were revealed in gait without poles (shoulder rotation RT, wrist radial-ulnar LT, hip flexion-extension LT and RT, knee flexion-extension LT and RT, ankle inversion-eversion LT, and ankle abduction-adduction LT and RT), the least in gait with mechatronic poles (knee flexion-extension LT and RT, ankle dorsiflexion-plantarflexion LT, ankle inversion-eversion LT, and ankle abduction-adduction LT and RT). Conclusion: Statistical analysis revealed many differences in spatiotemporal and kinematic parameters in normal gait, as well as in gait with the classical and mechatronic poles, which allows the conclusion that the gait of females and males should be analyzed separately.

Neurosurgical shunt treatment of pediatric hydrocephalus: epidemiology and influencing factors on revision surgeries: a single-center retrospective analysis of 131 patients.

BACKGROUND: Paediatric hydrocephalus is a result of a dysfunction of cerebrospinal fluid circulation, and it has diverse pathogeneses. This study investigates the epidemiology of paediatric hydrocephalus, as well as the influences of primary aetiology and implant type on treatment complications and the development of new therapeutic approaches and strategies. METHODS: Between 2013 and 2018, a retrospective analysis of 131 children, who were suffering from hydrocephalus, was conducted. Medical charts, operative reports and clinical follow-up visits were reviewed. Statistical analysis was performed using t-test/ANOVA and Kruskal-Wallis test/Mann-Whitney U test. RESULTS: The most common pathogeneses of hydrocephalus among our patients were meningomyelocele-associated and posthaemorrhagic. The majority of patients received a programmable differential pressure valve (PPV, 77.8%) or a fixed differential pressure valve with a gravitational unit (FPgV, 14.8%). Among 333 shunt-associated surgeries, 66% of surgeries were revision surgeries and were performed because of mechanical shunt dysfunction (61%), infection (12%), or other reasons (27%). The median rate of revisions within one year for each patient was 0.15 (IQR25-75: 0.00-0.68) and was influenced by aetiology (p = 0.045) and valve type (p = 0.029). The highest rates were seen in patients with posthaemorrhagic hydrocephalus and in those with FPgVs; the lowest rates were seen in patients with meningomyelocele-associated hydrocephalus and PPVs. The occurrence of mechanical dysfunctions was correlated with FPgV patients (p = 0.014). Furthermore, the median time interval between initial shunt surgery and onset of infection was shorter than that between initial surgery and mechanical dysfunction (p = 0.033). CONCLUSIONS: Based on this research, we can state several factors that influence revision surgeries in paediatric shunt patients. With the assessment of patients' risk profiles, physicians can classify paediatric shunt patients and thus avoid unnecessary examinations or invasive procedures. Furthermore, medical providers can prevent revision surgeries if they choose shunt material in accordance with a patient's associated shunt complications.

A prospective randomized study comparing retractor-endoscopic vs. open release of carpal tunnel and cubital tunnel syndromes.

OBJECTIVE: To evaluate the neurological and neurophysiological outcomes of retractor-endoscopic versus open release in carpal tunnel syndrome (rCTS and oCTS, respectively) and cubital tunnel syndrome (rCbTS and oCbTS, respectively) at 3- and 12-month follow-up. METHODS: Between 2013 and 2017, 80 patients were prospectively blindly randomized. McGowan scores were used for preoperative grading and outcomes were assessed using a modified Bishop rating system (BRS). Furthermore, incapacity to work, duration of postoperative pain, hypoesthesia, atrophy, subjective weakness, and a subjective assessment of the operative result were analyzed. The differences in the cohorts were evaluated with t-tests and ANOVAs as parametric tests and Kruskal-Wallis and Mann-Whitney U tests as nonparametric tests. RESULTS: The 80 patients underwent retractor-endoscopic or open decompression of the median or ulnar nerve. The rCTS group exhibited significant improvements in neurophysiological data (P = 0.032), shorter periods of postoperative pain (P = 0.03), and less discomfort (P = 0.005), as well as significantly better BRS results after 3 months compared with the oCTS group (P = 0.005). Between the oCbS and rCbTS groups, no significant differences were observed (P > 0.05). Regarding improvements in McGowan scores, no statistically significant differences were observed between the rCTS and oCTS groups after 3 months (P = 0.52) or 12 months (P = 0.86), nor were any observed between the rCbTS and oCbTS groups after 3 months (P = 0.88) or 12 months (P = 0.10). CONCLUSION: Significantly superior results were obtained at short-term follow-up for rCTS, whereas no superiority was found for rCbTS release. This study concluded that this endoscopic procedure is safe as well as and effective and has the potential to achieve better results in carpal tunnel syndrome compared with conventional methods.

ZEB1 induces N-cadherin expression in human glioblastoma and may alter patient survival.

The present study investigated the expression of epithelial-mesenchymal transition (EMT)-related factors zinc finger E-box-binding homeobox 1 (ZEB1), cadherin-1 (CDH1), cadherin-2 (CDH2) and the cell cycle modulating kinase cyclin-dependent kinase 1 (CDK1) in human glioblastoma (GBM) compared to normal brain tissue, as well as whether the levels of expression were associated with the overall and progression-free survival of the GBM patients. In 44 GBM and five normal brain tissue specimens, the expression levels of ZEB1, CDH1, CDH2 and CDK1 were evaluated by real-time PCR and immunostaining, and the results were correlated with clinical data. The expression levels of all investigated genes as detected by immunostaining were significantly higher in the GBM when compared to the normal brain tissues. There was no influence on survival. A linear correlation between ZEB1 and CDH2 and CDK1 expression was observed in GBM. Moreover, ZEB1 was involved in EMT (e.g., signaling in human GBM) and high ZEB1 levels were linked to an aberrant cell cycle processing, marked by CDK1 overexpression.

Secular Changes in the Age of Menarche of Rural and Urban Girls from an Industrial Region of Poland in Relation to Family Structure.

PURPOSE: The consequence of better living conditions for every subsequent generation is the phenomenon of change in the rate of maturation and body dimensions. The aim of this study was to evaluate the intergenerational changes in the age of menarche of girls living in rural and urban communities in the industrial region of Poland using samples from two different centuries and to determine the relationship between family structure (two-parent and single-parent) and sexual maturation of the girls in both environments. METHODS: The study included 3643 rural and urban girls aged 7-16 from southwestern Poland (Copper Basin). The research was cross-sectional in each environment around 2000 and 2010, and it was carried out twice. Two types of family structure were taken into account: two-parent and single-parent families. The median age of menarche and odds ratio (OR) of menarche depending on the place of residence and family structure were calculated. The status quo method was used when assessing the age of menarche. RESULTS: In both groups, the age of menarche significantly decreased during the decade. The median age of menarche in the urban girls was lower compared with their rural peers, but a significant difference was found only during the second examination (a decade after the first examination). There were no significant changes in menarche during the decade regarding family structure (neither in girls from two-parent families nor those from single-parent families), except in rural girls from two-parent families. Comparing the median age of menarche of girls from two-parent families with girls from single-parent families (urban and rural) showed lower median values in the girls from two-parent families, but no differences were significant. CONCLUSIONS: The acceleration of the maturation rate over the last decade has been observed among both rural and urban girls. Environmental differences in maturation rates between rural and urban girls increased over the course of the decade, and the difference between the rural and urban girls' age of menarche was statistically significant in the second examination (around 2010).The living conditions related to family structure did not significantly affect the timing of menarche.

The Use of the Bioelectrical Impedance Phase Angle to Assess the Risk of Sarcopenia in People Aged 50 and above in Poland.

PURPOSE: The increasing aging of many populations requires a continuous evolution of assessment methods in geriatrics, especially methods for identifying sarcopenia. Early diagnosis of unfavorable changes in the condition of skeletal muscles and the implementation of therapeutic methods may reduce the risk of functional limitations in the elderly. The aim of the study was to evaluate the association between the bioelectrical impedance phase angle and the occurrence of pre-sarcopenia in people aged 50 and above. METHODS: 1567 people aged 50-87 were examined. Anthropometric as well as muscle strength and walking speed measurements were performed. Using bioelectrical impedance analysis, the phase angle was measured and the appendicular skeletal muscle mass was estimated. The contribution of the phase angle in explaining the probability of the occurrence of pre-sarcopenia was verified by multivariate logistic regression. RESULTS: Sarcopenia was diagnosed in 12 people (0.8%) and pre-sarcopenia in 276 people (17.6%). Significantly lower impedance phase angle and muscle functional quality were found in people with confirmed pre-sarcopenia compared to people without sarcopenia. The relative differences for the phase angle were greater than for the indicator of muscle functional quality. Significant logit models were obtained for the probability of occurrence of pre-sarcopenia, in which the strongest predictor was the phase angle, regardless of the type and number of covariates. The cut-off point of the phase angle for identification of pre-sarcopenia was 5.42° in men and 4.76° in women. CONCLUSION: The strong association between the risk of pre-sarcopenia and the phase angle, which can be easily and quickly assessed by bio-impedance analysis, suggests the necessity to include this parameter in routine geriatric evaluation in order to identify the risk of sarcopenia.

Selected Elements of Lifestyle and the Level of Functional Fitness in Older Women.

BACKGROUND: The appropriate level of functional fitness is a very important element for seniors to maintain self-reliance in daily life. The aim of this research was to assess sociodemographic differences, selected elements of lifestyle, and functional fitness in the older residents of social welfare homes and community dwellers Methods: The analysed group comprised 693 women aged 65-79, including 173 subjects living in social welfare homes and 520 community-dwelling women. Basic anthropometric features were measured, and functional fitness was assessed using the Senior Fitness Test. Basic sociodemographic characteristics, as well as data on health self-assessment and selected elements of lifestyle, were also collected. RESULTS: The female residents of social welfare homes were found to have a lower body mass index (BMI), and they came from smaller cities, compared with community-dwelling older women. Furthermore, almost a third of them had no children and completed primary or vocational education. They also reported smoking, poor health conditions, and lack of physical activity. The functional fitness of women living in social welfare homes was significantly lower than in community-dwelling women. CONCLUSIONS: As seniors living in social welfare homes have such a significantly reduced level of functional fitness, compared with their peers living independently, it is necessary to include them in adaptive physical activity and diversified daily activities.

Tumor treating fields therapy is feasible and safe in a 3-year-old patient with diffuse midline glioma H3K27M - a case report.

Since high grade gliomas are aggressive brain tumors, intensive search for new treatment options is ongoing. For adult patients with newly diagnosed (ndGBM) and recurrent glioblastoma (rGBM), low intensity intermediate frequency alternating electric fields, known as tumor treating fields (TTFields) have been established as a new treatment modality. Tumor treating fields significantly increase survival rates in combination with adjuvant temozolomide (TMZ) in adult and GBM patients. Here, we report about feasibility and safety of treatment on a pediatric patient with diffuse midline glioma who is receiving TTFields therapy in combination with temozolomide.

Skeletal muscle quality in 6- and 7-y-old children assessed using bioelectrical impedance analysis.

OBJECTIVES: The condition of skeletal muscles is a key marker of the nutritional status and function of an organism. It is necessary to monitor muscle quality in basic pediatric preventive health care, especially due to the increase in negative health behaviors during a pandemic. The aims of this study were to assess body composition and muscle strength as well as to analyze the relationship between muscle functional quality and impedance parameters in 6- and 7-y-old children. METHODS: The study involved 292 healthy 6- and 7-y-old children. Handgrip strength and bioimpedance parameters were measured. Body composition components, including appendicular skeletal muscle mass, were estimated. Handgrip strength in relation to the appendicular skeletal muscles mass was adopted as an indicator of the muscle functional quality. The relationship between the muscle quality index and impedance parameters was assessed by multiple regression. RESULTS: A 1-y age difference between the children differentiated not only basic somatic parameters, body composition, and handgrip strength, but also the electrical properties of the tissues. The relative difference in muscle mass between younger and older children was twice that of the muscle strength. A significant regression model of the muscle quality index was obtained, in which reactance and impedance phase angle were strong positive predictors. Adjusted fat mass negatively correlated with the muscle quality. CONCLUSIONS: Reactance and phase angle are good indicators of the quality of appendicular skeletal muscle in healthy children and can be potentially used in pediatric preventive health care and screening.

Appendicular skeletal muscle mass and quality estimated by bioelectrical impedance analysis in the assessment of frailty syndrome risk in older individuals.

BACKGROUND AND AIM: The rising aging index of many populations necessitates the continuous evolution of geriatric assessment methods, especially the ones used to identify frailty and the risk of frailty. An appropriately early diagnosis of adverse changes in skeletal muscles can reduce the risk of functional limitations in elderly persons. The aim of this study was to assess the correlation between the appendicular skeletal muscle mass and quality, estimated by the bioelectrical impedance analysis method, and the risk of prevalence of the pre-frailty state in elderly persons. METHODS: One-thousand-and-fifteen subjectively healthy persons aged 60-87 years were tested. Anthropometric measurements and physical fitness and activity measurements were carried out and the frailty phenotype was evaluated. Appendicular skeletal muscle mass was estimated using the bioelectrical impedance analysis method. Muscle quality was assessed through an index correcting strength relative to muscle mass and through the impedance phase angle. The correlation between the muscle mass and quality estimating parameters and the probability of identifying pre-frailty was checked using multiple logistic regression. RESULTS: The prevalence of pre-frailty was 38%. The pre-frail persons were found to have a significantly lower muscle mass and quality than the non-frail persons, with the difference in the case of the muscle quality index nearly twice larger than for the muscle mass index. A significant logit model was obtained for pre-frailty prevalence, which was strongly dependent on the appendicular skeletal muscle mass (adjusted odds ratio (OR): 0.43, 95% CI 0.36-0.52, p < 0.001) and functional quality (adjusted OR: 0.26, 95% CI 0.18-0.38, p < 0.001) and less on age (adjusted OR: 1.10, 95% CI 1.07-1.13, p < 0.001). CONCLUSION: The strong correlation between the frailty phenotype and appendicular skeletal muscle mass and functional quality suggests that the two variables should be included in routine geriatric assessment with regard to frailty.

No Influence of Mechatronic Poles on the Movement Pattern of Professional Nordic Walkers.

This study compared selected temporal and kinematic parameters of normal gait and Nordic Walking (NW) performed with classic and mechatronic poles (classic poles equipped with sensors). It was assumed that equipping NW poles with sensors for biomechanical gait analysis would not impair the NW walking technique. Six professional NW instructors and athletes, including three women, participated in the study. The MyoMotion MR3 motion analysis system was used to collect gait kinematic variables. The subject's task was to cover a 100-m distance with three types of gait: a gait without poles, a gait with classic NW poles, and a gait with mechatronic poles at the preferred speed. Parameters were measured both on the right and left sides of the body. No significant differences were found between gait types for three temporal parameters: step cadence, step, and stride time. For the other variables, all the differences identified were between free-walking and walking with poles, with no differences between standard and mechatronic poles. For nine kinematic parameters, differences between free-walking and walking with poles for both the left and right sides were found, while no differences were due to the pole type. All temporal parameters were characterized by symmetry, while among kinematic parameters, only two were asymmetrical (shoulder abduction-adduction in walking with regular poles and elbow flexion-extension in walking without poles). Equipping classic NW poles with additional signaling and measuring devices (mechatronic poles) does not impair the NW technique, making it possible to use them in further studies of gait biomechanics.

Antiproliferative effect of GTS-21 in glioblastoma cells.

Glioblastoma multiforme (GBM) is the most common malignant brain tumour in adults. The poor prognosis and short median overall survival of patients with GBM is associated with resistance to therapy after surgical and adjuvant treatment. The expression of various acetylcholine receptors (AChR) in GBM has been widely reported. The present study aimed to investigate the expression of cholinergic system-related genes in primary GBM and to explore the antiproliferative effect of 3-(2,4-dimethoxybenzylidene) anabaseine (GTS-21) in GBM cell lines. Therefore, the expression of 28 genes associated with the cholinergic system was detected using a customized RT2 Profiler PCR Array in 44 GBM and 5 healthy control brain tissue samples. In addition, the activity of GTS-21, an alpha 7 subunit nicotinic AChR (α7 nAChR) agonist, and that of α-bungarotoxin (α-BTX), an α7 nAChR antagonist, was determined in primary and established GBM cells. Therefore, the A172, U87 and G28 cell lines and primary GBM cells were treated with GTS-21, ACh or nicotine. Cell viability was evaluated using MTT assay at 24, 48 and 72 h following cell treatment with the corresponding compounds. The results revealed that the expression of cholinergic system-related components was notably downregulated, except that of cholinergic receptor nicotinic alpha 7 subunit (CHRNA7), in primary GBM and U87 cells. However, the dominant-negative duplicate form of CHRNA7 was also downregulated. Furthermore, A172 and G28 cells exhibited a heterogeneous gene expression pattern. Additionally, GTS-21 inhibited the proliferation of GBM cells in a dose- and time-dependent manner. Interestingly, treatment with α-BTX restored the proliferation of U87 cells, but not that of A172 and G28 cells. Collectively, the findings of the present study suggested that GTS-21 may inhibit the proliferation of GBM cells and may therefore serve as a novel therapeutic approach to the treatment of GBM, which warrants further investigation.

Annual changes in appendicular skeletal muscle mass and quality in adults over 50 y of age, assessed using bioelectrical impedance analysis.

OBJECTIVES: It is essential to monitor changes in skeletal muscles to control healthy aging. The aim of this study was to assess changes in muscle mass, strength, and functional quality taking place in the course of aging and to analyze their correlations with recorded changes in impedance. METHODS: The study, carried out in two stages at a 1-y interval, covered 313 subjectively healthy adults ages 50 to 83 y. Hand grip strength and knee extensor strength were measured. Impedance parameters were measured using bioelectrical impedance analysis, and body composition components, including appendicular skeletal muscle mass, were estimated. The rates of annual changes in the analyzed parameters and their correlations with changes in impedance were calculated. RESULTS: The annual losses in muscle strength and functional quality were found to be considerably larger than the losses in appendicular skeletal muscle mass. Larger decreases in leg muscle strength were recorded in women than in men. The decline in reactance and phase angle were positively correlated with the decrease in the indices evaluating muscle condition. CONCLUSIONS: Muscle mass deficits are not the main cause of the decline in muscle functional quality in older adults. Changes in muscle condition impairing muscle functions can be identified by changes in impedance components. An analysis of changes in phase angle and reactance would facilitate the assessment of muscle quality in geriatric prophylaxis and in screening, but it is necessary to establish normalized reference values for older adults.