[METABOLIC ACTIVITY OF LUNG AT EXPERIMENTAL BRAIN ISCHEMIA IN CONDITION OF CAPSAICIN BLOCKADE OF VAGUS NERVE].

In rats with ligation of both common carotid arteries in condition of bilateral capsaicin blockade of vagus nerve the metabolic functions of the lungs associated with their participation in the regulation of hemostasis, lipid composition of blood plasma and lung surfactant were investigated. It is shown that cerebral ischemia is accompanied by changes in the fractional composition of phospholipids in both arterial and venous blood, as well as in pulmonary surfactant, and the decrease of the lung hypocoagulation effect. The phagocytic activity of alveolar macrophages (as one of the factors involved in surfactant lipids metabolism) in brain ischemia is reduced. After the blockade of capsaicin-sensitive vagal afferents the changes in surfactant and plasma phospholipids caused by cerebral ischemia, are abolished, hypocoagulation effect of lung restores, but the functional activity of macrophages remains decreased. These findings suggest that capsaicin-sensitive vagal afferents are involved in the development of disregulatory disorders of lung metabolic function in cerebral ischemia.

[BINDING OF CELL-DERIVED MICROPARTICLES WITH FIBRIN IN BLOOD CLOTTING].

Direct effects of circulating blood microparticles on fibrin formation and structure were studied. Clots made from platelet-free plasma and from microparticle-depleted plasma obtained by filtration was studied in parallel, including clots from the microparticle-depleted plasma replenished with phospholipids. Fibrin formation was induced by exogenous thrombin without Ca2+ to prevent formation of endogenous thrombin and exclude indirect kinetic effects of microparticles related to thrombin generation. In the presence of natural microparticles or exogenous phospholipids the maximal turbidity of fibrin clots was significantly smaller, indicating structural distinctions from the clots formed in the absence of microparticles. Scanning electron microscopy and confocal microscopy showed that clots formed from platelet-free plasma, i. e. in the presence of microparticles, unlike clots from the microparticle-depleted plasma, contained 0.1-0.5-µm-large CD61-positive granules associated with fibrin fibers that were identical to the particles found on the surface of filters used for microparticle removal. The results show that platelet-derived microparticles bind to fibrin and affect its structure. The revealed interactions of cell-derived microparticles with fibrin highlight a previously unknown role of microparticles in hemostasis and thrombosis as constituents and modulators of a fibrin clot structure.

[EFFECTS OF LIGHT OR DARK DEPRIVATION ON PERFORMANCE AND LACTATE LEVELS IN RAT BLOOD].

The dynamics of rat's swimming test with a load after 10 daily deprivation of light or dark were investigated. It was established that in the control group in nature illumination daily physical swimming with a load to full exhaustion from the first to the third day of the experiment, there was an increase of active swimming time indicator followed by stabilization. In groups of animals kept before bringing swimming test in a day and night lighting or darkening around the clock, there was an increase in swimming time only on the first day of the experiment, and the next days there was a decrease of efficiency. After a five-day cycle of the swimming load the level of lactate in the blood of control animals was increased, and experienced - has not changed in comparison with the intact, not subjected to any stress.