RESUMEN
The RNA world hypothesis suggests that chemical networks consisting of functional RNA molecules could have constructed a primitive life-like system leading a first living system. The chemical evolution scenario of RNA molecules should be consistent with the Hadean Earth environment. We have demonstrated the importance of the environment at both high temperature and high pressure, using different types of hydrothermal flow reactor systems and high-pressure equipment. In the present study, we have attempted to develop an alternative easy-to-implement method for high-pressure measurements and demonstrate that the system is applicable as an efficient research tool for high-pressure experiments at pressures up to 30 MPa. We demonstrate the usefulness of the system by detecting the high-pressure influence for the self-cleavage of avocado hammerhead ribozyme (ASBVd(-):HHR) at 45-65 °C. A kinetic analysis of the high-pressure behavior of ASBVd(-):HHR shows that the ribozyme is active at 30 MPa and its activity is sensitive to pressures between 0.1-30 MPa. The surprising finding that such a short ribozyme is effective for self-cleavage at a high pressure suggests the importance of pressure as a factor for selection of adaptable RNA molecules towards an RNA-based life-like system in the Hadean Earth environment deep in the ocean.
RESUMEN
In a previous study, we have showed that the elongation of an alanine oligopeptide [L-alanyl-L-alanyl-L-alanyl-L-alanine ((Ala)4)] to higher oligopeptides is enhanced by calcite and dolomite at 275°C, using a mineral-mediated hydrothermal flow reactor system. However, a problem during the use of hydrothermal flow reactor system was that some of the minerals, such as clay, could not be tested due to their clogging in the reactor. In this article, we attempted to analyze the scope of enhancement for the formation of L-alanyl-L-alanyl-L-alanyl-L-alanyl-L-alanine ((Ala)5) and higher oligopeptides with different minerals including clay minerals for the elongation of alanine oligopeptide at 175°C. First, carbonate minerals and some clay minerals showed an enhancement of the formation of (Ala)5 from (Ala)4. On the contrary, volcanic products showed strong inhibitory activities. According to the pH dependence on the (Ala)4 elongations, we confirmed that most enhancement and inhibitory activities are due to the pH influence on the elongation of (Ala)4. However, the enhancement of montmorillonite (Tsukinuno), sphalerite, apatite, tourmaline, calcite (Nitto Funka), and the inhibitory activities by volcanic ash (Shinmoedake), volcanic ash (Sakurajima), dickite, and pyrophillite are not simply due to the pH change in the presence of these minerals. The difference found between the previous and present studies suggests that the interaction kinetics of the aqueous phase with the mineral phase is also an important factor for the elongation of (Ala)4. These data imply that the environments with pH near neutral to weak alkaline and with minerals might have been useful for the accumulation of oligopeptides in hydrothermal conditions.
