RESUMEN
We studied the sensitivity of domestic proprietary human and animal cell lines from the collection of M. P. Chumakov Federal Scientific Center for Research and Development of Immuneand-Biological Products to infection with different enterovirus 71 strains. A cell system based on domestic proprietary permanent cell line 4647 was for the first time used for reproduction of four enterovirus 71 strains (BrCr, 42266, 42934, and 43374). It was shown that strain 4647 is the optimal cell substrate for enterovirus 71 reproduction. The titers of enterovirus 71 for all four strains considerably (by 2 lgTCID50/ml and more) increased during sequential passages in permanent cell line 4647. The prospects of using permanent cell line 4647 for creation of diagnostic and preventive preparations against 71 was demonstrated.
Asunto(s)
Enterovirus Humano A/fisiología , Células Epiteliales/virología , Células Musculares/virología , Replicación Viral , Animales , Línea Celular , Chlorocebus aethiops , Células Epiteliales/patología , Humanos , Células Musculares/patología , Carga ViralRESUMEN
We performed morphofunctional analysis of cultured rabbit buccal epithelial cells in the primary culture, during passaging, and upon interaction with collagen substrates. Three different morphological types were identified. It was demonstrated that precursors of rabbit buccal epithelium were characterized by high proliferative activity, retain their biological properties throughout long-term culturing, and adhere to various collagen substrates.
Asunto(s)
Tratamiento Basado en Trasplante de Células y Tejidos/métodos , Colágeno/farmacología , Células Epiteliales/citología , Mucosa Bucal/citología , Cultivo Primario de Células/métodos , Animales , Cámara Anterior/patología , Técnicas de Cultivo de Célula , Proliferación Celular , Separación Celular , Células Cultivadas , Úlcera de la Córnea/terapia , ConejosRESUMEN
Serum concentration of platelet-derived growth factor was measured in patients with trophic ulcers and proliferative activity of human fibroblasts was measured in wells with different content of added serum platelet-derived growth factor. In parallel, morphofunctional analysis of platelets from the whole blood of these patients was performed. A close correlation was found between morphofunctional parameters of blood platelets and serum concentration of platelet-derived growth factor. Platelet-derived growth factor in concentrations below 150 pg per well stimulated fibroblast proliferation and preserved their viability. Platelet-derived growth factor in concentrations >200 pg per well suppressed fibroblast proliferation and impaired their viability.
