MALDI imaging mass spectrometry reveals multiple clinically relevant masses in colorectal cancer using large-scale tissue microarrays.

For identification of clinically relevant masses to predict status, grade, relapse and prognosis of colorectal cancer, we applied Matrix-assisted laser desorption ionization (MALDI) imaging mass spectrometry (IMS) to a tissue micro array containing formalin-fixed and paraffin-embedded tissue samples from 349 patients. Analysis of our MALDI-IMS data revealed 27 different m/z signals associated with epithelial structures. Comparison of these signals showed significant association with status, grade and Ki-67 labeling index. Fifteen out of 27 IMS signals revealed a significant association with survival. For seven signals (m/z 654, 776, 788, 904, 944, 975 and 1013) the absence and for eight signals (m/z 643, 678, 836, 886, 898, 1095, 1459 and 1477) the presence were associated with decreased life expectancy, including five masses (m/z 788, 836, 904, 944 and 1013) that provided prognostic information independently from the established prognosticators pT and pN. Combination of these five masses resulted in a three-step classifier that provided prognostic information superior to univariate analysis. In addition, a total of 19 masses were associated with tumor stage, grade, metastasis and cell proliferation. Our data demonstrate the suitability of combining IMS and large-scale tissue micro arrays to simultaneously identify and validate clinically useful molecular marker. Copyright © 2017 John Wiley & Sons, Ltd.

Emerging technologies for bioweapons defense.

The Global War on Terrorism (GWOT) has changed the way we think about national security. The tragic events of Sept.11 have shown us that world of the 21(st) century is clearly a dangerous place. Terrorism, more than ever, affects the very lives of each and every citizens of our country. Al Qaida has proven that it is able to use simple rudimentary methods to invoke strategic economic losses to our society with our own domestic resources as its weapons. The dawn of the new century has brought forth a new kind of asymmetric war where guerilla fighters are armed not with rifles but with technology at the touch of a keyboard. Warfare as we know it has changed and just as the military has its "transformation", medicine so too must have its own transformation in order to protect our citizen against the ever changing threat of bioterrorism. Virtual Reality and its applications can play a vital role in developing new countermeasures to minimize the catastrophic effects of a potential bioterror attack.

Homeland security and virtual reality: building a Strategic Adaptive Response System (STARS).

The advent of the Global War on Terrorism (GWOT) underscored the need to improve the U.S. disaster response paradigm. Existing systems involve numerous agencies spread across disparate functional and geographic jurisdictions. The current architecture remains vulnerable to sophisticated terrorist strikes. To address these vulnerabilities, we must continuously adapt and improve our Homeland Security architecture. Virtual Reality (VR) technologies will help model those changes and integrate technologies. This paper provides a broad overview of the strategic threats, together with a detailed examination of how specific VR technologies could be used to ensure successful disaster responses.

Reagan, Clinton, tobacco, and children: an interview with C. Everett Koop.

At eighty-seven years of age, former Surgeon General C. Everett Koop has an extraordinary perspective on health and medicine in America. He reflects on child health from his thirty-five years as a pediatric surgeon; AIDS from his position as Surgeon General at the outset of the epidemic; Presidents Reagan and Clinton, with whom he worked extensively; and smoking from his long battle first in government and then later as public antagonist to what he sees as the duplicitous and deceptive "killer" industry: tobacco.