RESUMEN
In direct experiments, rate constants of photochemical (kP) and non-photochemical (kP(+)) fluorescence quenching were determined in membrane fragments of photosystem II (PSII), in oxygen-evolving PSII core particles, as well as in core particles deprived of the oxygen-evolving complex. For this purpose, a new approach to the pulse fluorometry method was implemented. In the "dark" reaction center (RC) state, antenna fluorescence decay kinetics were measured under low-intensity excitation (532 nm, pulse repetition rate 1 Hz), and the emission was registered by a streak camera. To create a "closed" [P680(+)QA(-)] RC state, a high-intensity pre-excitation pulse (pump pulse, 532 nm) of the sample was used. The time advance of the pump pulse against the measuring pulse was 8 ns. In this experimental configuration, under the pump pulse, the [P680(+)QA(-)] state was formed in RC, whereupon antenna fluorescence kinetics was measured using a weak testing picosecond pulsed excitation light applied to the sample 8 ns after the pump pulse. The data were fitted by a two-exponential approximation. Efficiency of antenna fluorescence quenching by the photoactive RC pigment in its oxidized (P680(+)) state was found to be ~1.5 times higher than that of the neutral (P680) RC state. To verify the data obtained with a streak camera, control measurements of PSII complex fluorescence decay kinetics by the single-photon counting technique were carried out. The results support the conclusions drawn from the measurements registered with the streak camera. In this case, the fitting of fluorescence kinetics was performed in three-exponential approximation, using the value of τ1 obtained by analyzing data registered by the streak camera. An additional third component obtained by modeling the data of single photon counting describes the P680(+)Pheo(-) charge recombination. Thus, for the first time the ratio of kP(+)/kP = 1.5 was determined in a direct experiment. The mechanisms of higher efficiency for non-photochemical antenna fluorescence quenching by RC cation radical in comparison to that of photochemical quenching are discussed.
Asunto(s)
Radicales Libres/química , Complejo de Proteína del Fotosistema II/metabolismo , Arabidopsis/metabolismo , Cationes/química , Ditionita/química , Cinética , Complejo de Proteína del Fotosistema II/química , Hojas de la Planta/metabolismo , Espectrometría de FluorescenciaRESUMEN
In a direct experiment, the rate constants of photochemical k p and non-photochemical k p+ quenching of the chlorophyll fluorescence have been determined in spinach photosystem II (PS II) membrane fragments, oxygen-evolving PS II core, as well as manganese-depleted PS II particles using pulse fluorimetry. In the dark-adapted reaction center(s) (RC), the fluorescence decay kinetics of the antenna were measured at low-intensity picosecond pulsed excitation. To create a "closed" P680+Q A- state, RCs were illuminated by high-intensity actinic flash 8 ns prior to the measuring flash. The obtained data were approximated by the sum of two decaying exponents. It was found that the antennae fluorescence quenching efficiency by the oxidized photoactive pigment of RC P680+ was about 1.5 times higher than that of the neutral P680 state. These results were confirmed by a single-photon counting technique, which allowed to resolve the additional slow component of the fluorescence decay. Slow component was assigned to the charge recombination of P680+Pheo- in PS II RC. Thus, for the first time, the ratio k p+ /k p â 1.5 was found directly. The mechanism of the higher efficiency of non-photochemical quenching comparing to photochemical quenching is discussed.
Asunto(s)
Complejo de Proteína del Fotosistema II/metabolismo , Cationes/metabolismo , Clorofila/metabolismo , Fluorescencia , Radicales Libres/metabolismo , Cinética , Complejos de Proteína Captadores de Luz/metabolismo , Oxígeno/metabolismo , Spinacia oleracea/metabolismoRESUMEN
The fluorescence lifetime and quantum yield as a function of single picosecond laser pulse intensity were experimentally studied in chloroplasts and subchloroplast particles. The intensity of laser pulse were changed from 3 x 10(12) to 10(17) photon/cm2. To explain experimental results it was considered that energy transport in the light harvesting antenna occurs by localised excitons. Parameters governing dynamic of electronic excitation within light harvesting antenna were determined. The diffusion coefficient was found to be 2 x 10(-2) cm2sec-1; diffusion length L greater than or equal to 900 A, energy transfer probability W approximately 10(12)sec-1. The rate of constant of energy transfer from light harvesting antenna to PS1 and PS2 reaction centers and effectiveness of the exciton capture by the PS2 reaction center were estimated.