RESUMEN
In Drosophila, the BEAF-32, Z4/putzig, and Chriz/Chromator proteins colocalize in the interbands of polytene chromosomes. It was assumed that these proteins can form a complex that affects the structure of chromatin. However, the mechanism of the formation of such a complex has not been studied. We have proved for the first time that the BEAF-32, Z4/putzig, and Chriz/Chromator proteins interact directly with each other and localized the protein domains that provide multiple protein-protein interactions. Based on the data obtained, we developed a model of the mechanism of the formation the BEAF/Z4/Chriz complex and its recruitment to chromatin.
Asunto(s)
Proteínas de Ciclo Celular/metabolismo , Cromatina/metabolismo , Proteínas de Unión al ADN/metabolismo , Proteínas de Drosophila/metabolismo , Drosophila melanogaster/metabolismo , Proteínas del Ojo/metabolismo , Proteínas Asociadas a Matriz Nuclear/metabolismo , Animales , Proteínas de Ciclo Celular/genética , Cromatina/genética , Proteínas de Unión al ADN/genética , Proteínas de Drosophila/genética , Drosophila melanogaster/genética , Proteínas del Ojo/genética , Proteínas Asociadas a Matriz Nuclear/genética , Cromosomas Politénicos , Dominios y Motivos de Interacción de ProteínasRESUMEN
Proteins Z4/putzig and Chriz/Chromator are involved in the chromatin organization on the promoters of the majority of Drosophila genes. It was shown that the Chriz protein region from aa 273 to 503 is required for the interaction with the Z4 protein. Deletion of this sequence leads to derepression of a number of STAT-dependent genes and development of melanotic tumors in flies. The results of this study suggest that the Chriz protein promotes the recruitment of the Z4 protein to chromatin.
Asunto(s)
Proteínas de Ciclo Celular/metabolismo , Proteínas de Drosophila/metabolismo , Drosophila melanogaster/genética , Regulación de la Expresión Génica , Proteínas Asociadas a Matriz Nuclear/metabolismo , Factores de Transcripción STAT/metabolismo , Animales , Sitios de Unión , Cromatina/química , Proteínas de Unión al ADN/metabolismo , Drosophila melanogaster/metabolismo , Eliminación de Gen , Cromosomas Politénicos/metabolismo , Regiones Promotoras Genéticas , Unión Proteica , Factores de Transcripción STAT/genética , Técnicas del Sistema de Dos HíbridosRESUMEN
51 patients with operated colorectal cancer T1N0M0, T3N1M0 and T4N0M1 at the age of 67±2,3 years receiving adjuvant chemotherapy and nutritional support (NS) were examined. Nutritional status was assessed using alimentazione-volume diagnosis to the points on L.N.Kostyuchenko, nutritional risk - with NRI, body composition - with bioimpedance method, the iron metabolism - with basic markers (serum ferritin, transferrin saturation with iron, erythrocyte indices: erythrocyte saturation of iron, the average concentration of hemoglobin in the erythrocyte, mean corpuscular volume, hemoglobin, etc.), traditional settings, the staging of tumors - according to TNM. Iron deficiency before the development of anemia can be corrected with NS. Upon the occurrence of anemia requires additional pharmacological treatment iron supplementation, preferable with iron III hydroxide olygoisomaltazat 1000 + NS) for the prevention of toxic-metabolic complications.
Asunto(s)
Anemia Ferropénica , Neoplasias Colorrectales , Apoyo Nutricional , Anciano , Anemia Ferropénica/complicaciones , Anemia Ferropénica/tratamiento farmacológico , Neoplasias Colorrectales/complicaciones , Neoplasias Colorrectales/tratamiento farmacológico , Compuestos Férricos/administración & dosificación , Ferritinas , Humanos , Ácidos Hidroxámicos/administración & dosificación , Apoyo Nutricional/normas , SíndromeRESUMEN
It is found that, in embryonic D. melanogaster cells, Mod(mdg4) protein isoforms can interact with each other through BTB domains. However, this nonspecific interaction is destroyed as a result of recruitment of protein complexes to the chromatin sites.
Asunto(s)
Proteínas de Unión al ADN/metabolismo , Proteínas de Drosophila/metabolismo , Drosophila melanogaster/embriología , Embrión no Mamífero/citología , Proteínas de Unión al ARN/metabolismo , Animales , Proteínas de Unión al ADN/química , Proteínas de Drosophila/química , Embrión no Mamífero/metabolismo , Unión Proteica , Dominios Proteicos , Isoformas de Proteínas/química , Isoformas de Proteínas/metabolismo , Proteínas de Unión al ARN/químicaRESUMEN
It is shown that mutations in two GC-rich sequences (GC-boxes) from the promoter region of the yellow gene during enhancer-dependent transcription activation do not affect the basal level of the yellow gene transcription but destabilize the interaction between the enhancers and the promoter.
Asunto(s)
Elementos de Facilitación Genéticos , Secuencia Rica en GC/fisiología , Regiones Promotoras Genéticas , Animales , Drosophila melanogaster/genética , TATA Box/genética , Transcripción Genética/efectos de los fármacosRESUMEN
As a result of experiments in yeast two-hybrid system, coimmunoprecipitation of proteins from D. melanogaster embryo cell lysate, and immunostaining, it was shown for the first time that Mod(mdg4)-58.8 protein (isoform P), a product of mod(mdg4) locus, directly interacts with mtACP1A and mtACP1B proteins. These proteins are involved in de novo biosynthesis of fatty acids in mitochondria and are required for normal gametogenesis in males and females and, possibly, for the trachea development. This result expands the understanding of the role of mod(mdg4) locus products in the regulation of life activity of the eukaryotic cell.
Asunto(s)
Proteína Transportadora de Acilo/metabolismo , Proteínas de Drosophila/metabolismo , Drosophila melanogaster/genética , Proteínas Mitocondriales/metabolismo , Factores de Transcripción/metabolismo , Animales , Femenino , Masculino , Unión Proteica , Isoformas de Proteínas/metabolismoAsunto(s)
Transportadoras de Casetes de Unión a ATP/genética , Proteínas de Unión al ADN/genética , Proteínas de Drosophila/genética , Elementos de Facilitación Genéticos , Proteínas del Ojo/genética , ARN Polimerasa II/genética , Transportadoras de Casetes de Unión a ATP/metabolismo , Animales , Animales Modificados Genéticamente , Secuencia de Bases , Color , Ojo Compuesto de los Artrópodos/fisiología , Proteínas de Unión al ADN/metabolismo , Proteínas de Drosophila/metabolismo , Drosophila melanogaster , Proteínas del Ojo/metabolismo , Datos de Secuencia Molecular , Fenotipo , Regiones Promotoras Genéticas , ARN Polimerasa II/metabolismoRESUMEN
The article presents a review of acute surgical pathology and the frequency of its occurrence, complicating alcohol poisoning, cauterizing liquids, narcotic and psychotropic drugs and acute emerging metabolic (protein-energy). The development of surgical complications in poisoning was observed in patients in severe and very severe condition and is accompanied by a sharp increase in mortality (75%) and severe energy dysfunction. The most diverse and difficult flowing surgical complications were encountered in alcohol poisoning and cauterizing liquids.
Asunto(s)
Intoxicación , Complicaciones Posoperatorias , Deficiencia de Proteína , Enfermedad Aguda , Humanos , Intoxicación/metabolismo , Intoxicación/mortalidad , Intoxicación/cirugía , Complicaciones Posoperatorias/metabolismo , Complicaciones Posoperatorias/mortalidad , Complicaciones Posoperatorias/terapia , Deficiencia de Proteína/etiología , Deficiencia de Proteína/metabolismo , Deficiencia de Proteína/terapiaRESUMEN
Analysis and prevention of complications of parenteral and enteral nutrition recently receiving more attention. This is because a full infusion-nutritive correction ensures shorter postoperative period and can be used as the protector of metabolic damage, aggravating, and sometimes determining operating.
Asunto(s)
Nutrición Enteral/efectos adversos , Enfermedades Metabólicas/etiología , Enfermedades Metabólicas/prevención & control , Nutrición Parenteral/efectos adversos , Nutrición Enteral/métodos , Humanos , Enfermedades Metabólicas/metabolismo , Nutrición Parenteral/métodosRESUMEN
Migration of ascaris from the lumen of the small intestine to the biliary system was previously thought to be very rare pathology and the diagnosis had not be without specific examination methods. At the present time widespread active ultrasonic technologies in the emergency surgical service can became more accessible to show worm in a gall bladder and ducts. The paper presents two cases of gallbladder ascariasis, identified by ultrasound.