[The safety and protective properties of a new cell-free pertussis vaccine in animal trials]. / Issledovanie bezvrednosti i protektivnykh svoistv novoi beskletochnoi kokliushnoi vaktsiny v opytakh na zhivotnykh.

The preclinical trial of a new cell-free pertussis vaccine has been carried out with a view to its comparison with whole-cell pertussis vaccine in respect of the toxic and protective properties of the preparations under experimental conditions. The whole-cell pertussis vaccine is characterized by pronounced toxic action causing leukocytosis and histamine-sensitization, produces a negative effect on the detoxifying function of the liver and possesses sensitizing activity. The cell-free pertussis vaccine has pronounced protective activity. In animal experiments the supposed immunization dose of this vaccine has proved to be nontoxic.

[The use of pharmacological screening for characterizing the toxicity of pertussis vaccines]. / Ispol'zovanie farmakologicheskogo skrininga dlia kharakteristiki toksichnosti kokliushnykh vaktsin.

The influence of pertussis preparations, introduced by oral and parenteral routes, on the detoxifying function of the liver and the state of the nervous system of the animals was studied by methods used in pharmacology and toxicology. The use of these methods made it possible to find out side effects produced by corpuscular pertussis vaccine, introduced parenterally, on the detoxifying function of the liver and the state of the nervous system of the animals. The negative influence on the nervous system was more pronounced after the injection of the commercial adsorbed diphtheria-pertussis-tetanus vaccine used in this investigation than after the injection of pertussis monovaccine. The oral administration of corpuscular pertussis vaccine exerted no negative influence on the above-mentioned body functions of the animals.

[Comparative pathomorphologic study of the toxic properties of a corpuscular pertussis vaccine and of a cell-free pertussis preparation]. / Sravnitel'noe patomorfologicheskoe izuchenie toksicheskikh svoistv korpuskuliarnoi kokliushnoi vaktsiny i beskletochnogo kokliushnogo preparata.

The comparative study of morphological changes in the body of outbred mice under the action of corpuscular pertussis vaccine and acellular pertussis preparation has been made. The corpuscular vaccine has been shown to produce a pronounced, dynamically increasing toxic effect, thus causing the damage of lymphoid thymic and spleen cells, prolonged interstitial reaction in the lungs, destructive inflammatory process at the site of injection. The acellular pertussis preparation is less toxic, induces less pronounced changes in these organs at the early period of the experiment, stimulates the proliferation of lymphoid cells and lymphoblast transformation. As noted in this study, the damaging action of pertussis vaccine is mainly indicated by pathological phenomena appearing in the organs of the immune system, pulmonary parenchyma and muscular tissue (in the inoculation zone).

[Advantages of using pharmacologic tests to characterize bacterial vaccines]. / O tselesoobranznosti ispol'zovaniia farmakologicheskikh testov dlia kharakteristiki bakterial'nykh vaktsin.

The possibility of using pharmacological tests to determine side effects produced by adsorbed DPT vaccine and chemical typhoid vaccine was studied. Both vaccines were shown to be capable of prolonging sleep induced by hexenal or thiopental in experimental animals. The following difference in the action of these two preparations was revealed on the model of "chemical convulsions" induced by the injection of thiosemicarbazide: while immunization with DPT vaccine acitivated thiosemicarbazide-induced convulsive syndrome in mice, no such stimulating effect was observed after the injection of typhoid, vaccine. The study of pharmacological effects allowed to evaluate some aspects of the side effects producted by prophylactic preparations.