[Mechanisms of the therapeutic effect of immunoglobulins in the treatment of bronchial asthma].

Data on the therapeutic effectiveness of intravenous injections of immunoglobulins in high doses in patients with bronchial asthma are analyzed. Some possible mechanisms of this therapeutic effect are considered, which are related to of immunoglobulin fragments capable of interacting with cytochrome P-450 and NO-synthase systems of the liver.

[Effect of carnosine and its combination with essentiale on lipid peroxidation and work capacity of experimental animals].

The effects of carnosine and its combination with essentiale on the processes of lipid peroxidation (LPO) and on the work capacity have been studied in mice. The administration of camosine, essentiale, and vitamin E, increased the work capacity of laboratory mice to a different extent, the effect being most pronounced for a mixture of carnosine and essentiale. Carnosine in the dose range studied exhibited a pronounced antioxidant effect with respect to LPO and optimized the potential of the organism in the course of intensive physical activity. This drug is not a doping and can be recommended as a means of improvement of the physical capacity.

[Dyes with properties of modulators of redox processes as potential immunotropic agents]. / Krasiteli so svoistvami moduliatorov okislitel'no-vosstanovitel'nykh protsessov kak potentsial'nye immunotropnye sredstva.

We performed an analysis of the biological activity of dyes possessing certain redox potential. This provided a theoretical basis for the development of a new trend in pharmacology. We demonstrate the wide potential associated with the use of this group of substances to regulate various biochemical processes in the immunity system.

[The immunochemical mechanisms of body adaptation to a chemical environment]. / Immunokhimicheskie mekhanizmy adaptatsii organizma k okruzhaiushchei khimicheskoi srede.

For the first time the dynamics of metabolic and immune responses to xenobiotics were analyzed in their long-term effect on the organism. It was shown that the organism gradually lost the capacity to respond to xenobiotics with the induction of hepatic cytochrome P450 and immune response. Possible mechanisms of this phenomenon are under consideration.

[Cytochrome P-450 induction and the subsequent induction of an immune response in rats during the chronic administration of xenobiotics]. / Induktsiia tsitokhroma P-450 i posleduiushchaia induktsiia immunnogo otveta u krys pri khronicheskom vvedenii ksenobiotikov.

The long-term administration of xenobiotics carcinogens o-aminoazotoluene (o-AAT) and benz(a)pyrene (BP) to rats was found to cause induction of the liver cytochrome P-450 system which gradually decreases in spite of continued administration of the agents. Induction of microsomal oxygenases under these conditions is followed by induction of the immune response to o-AAT and BP. The data obtained correspond to the conception of the immunochemical functional system of homeostasis implying that the cytochrome-450 system and the immunity system are functionally linked and are elements of the common functional adaptive system of the organism.

[Immune response to benzo(a)pyrene in rabbits immunized with a conjugate of benzo(a)pyrene-albumin, synthesized in the microsomal monooxygenase system of the liver]. / Imunnyi otvet na benzo(A)piren u krolikov, immunizirovannykh kon''iugatom benzo(A)piren-al'bumin, sintezirovannym v mikrosomalnoi monooksigenaznoi sisteme pecheni.

The feasibility of obtaining a conjugated benz(a)pyrene-protein antigen in the liver cytochrome P-450 system was studied. Covalent binding of benz(a)pyrene (BP) to albumin was performed with the use of liver microsomal fractions of 3-methylcholanthrene-induced rabbits. It was demonstrated that BP oxidation in liver microsomes is accompanied by covalent binding of [14C]BP to exogenous rabbit albumin. Immunization of rabbits with the obtained conjugate results in the development of a specific immune response to BP, and the appearance of specific antibodies and lymphocytes specifically binding [14C]BP in the blood.

[Enzymatic synthesis and immunochemical characterization of conjugated morphine-protein antigens]. / Fermentativnyi sintez i immunokhimicheskaia kharakteristika kon'iugirovannykh antigenov morfin-belok.

Conjugates morphine-protein which are formed in the systems of cytochrome P-450 and horse-radish peroxidase can induce in rabbits synthesis of the specific antibodies to morphine. In the blood of animals immunized with the above mentioned conjugates there are antimorphine-antibodies being constituents on immunochemical complexes with endogenous ligands. Administration to rabbits of the conjugated antigen morphine-microsomes of the rabbit liver causes the development of the reaction of hypersensitivity of delayed type to morphine.

[Effect of glycine on liver cytochrome P-450 and severity of experimental food anaphylaxis]. / Vliianie glitsina na tsitokhrom P-450 i vyrazhennost' éksperimental'noi pishchevoi anafilaksii.

Male guinea pigs, intact and orally sensitized with chick ovalbumin (OVA), having initial bw 250-300 g, received glycine intraperitoneally or orally in a single dose of 100 or 200 mg/kg/day, during 3 days. The specific volume of cytochromes P450 and b5 in the microsomal fraction of the liver, activity of aminopyrine N-demethylase and aniline-p-hydroxylase, severity of food anaphylaxis to OVA and the animals' sensitivity to intravenous histamine LD50 were studied. The inducing effect of glycine on the cytochrome P450 system in the liver was more pronounced in the presensitized animals, that was attended by a decreased severity of food anaphylaxis and by a protective action in respect to histamine LD50. Possible mechanisms of glycine action are considered regarding its participation in metabolic processes.

[The immunotropic activity of GABA during cytochrome P-450 induction in the liver]. / Immunotropnaia aktivnost' GAMK v usloviiakh induktsii tsitokhroma P-450 v pecheni.

Gamma-aminobutyric acid (GABA) was shown to exert the immunodepressive effect. The use of GABA under induction of cytochrome P-450 of the liver by phenobarbital or rifampicin was not followed by immunodepression. The obtained data indicate the dependence of GABA pharmacological activity from the function of cytochrome P-450-dependent monooxygenase system of the liver.

[Modern principles and ways to develop immunopharmacology]. / Sovremennye printsipy i puti razvitiia immunofarmakologii.

The principles of coding in the organism of the information about an unlimited scope of substances and the formation of peptide analogues of the original nonpeptide chemical structures are first formulated on the basis of the conception of the immunochemical functional system of homeostasis and the data on the pharmacological activity of antibodies to low molecular organic compounds and the corresponding anti-antibodies.

[Cytochrome P-450-dependent mechanisms of the biosynthesis of protein-conjugated benzo(a)pyrene antigens and their role in the development of a specific immune response to this carcinogen]. / Izuchenie tsitokhrom-P-450-zavisimykh mekhanizmakh biosinteza kon''iugirovannykh antigenov benzo(a)pirena s belkami i ikh rol' v razvitii spetsificheskogo immunnogo otveta na étot kantserogen.

There was studied the possibility of covalent binding to proteins of a carcinogenic compound benzo(a)pyrene in the system of cytochrome P-450 of the liver and the possibility of the development of the immune reaction to administration of the conjugated antigens obtained in such a way to animals. It was shown that under experimental conditions modelling the processes of microsomal oxidation of benzo(a)pyrene in the organism there occurs irreversible binding of 14C-benzo(a)pyrene both to microsomes and the added albumin. Immunization of rabbits by conjugates benzo(a)pyrene-albumin and benzo(a)pyrene-microsomes leads to the development of the immune response to the original carcinogen. The antibodies and lymphocytes specifically binding 14C-benzo(a)pyrene appear in the blood.