Genotoxic effects in transformed and non-transformed human breast cell lines after exposure to silver nanoparticles in combination with aluminium chloride, butylparaben or di-n-butylphthalate.

In the present study genotoxic effects after combined exposure of human breast cell lines (MCF-10A, MCF-7 and MDB-MB-231) to silver nanoparticles (AgNP, citrate stabilized, 15 and 45nm by STEM, Ag15 and Ag45, respectively) with aluminium chloride, butylparaben, or di-n-butylphthalate were studied. In MCF-10A cells exposed for 24h to Ag15 at the concentration of 23.5µg/mL a statistically significant increase in DNA damage in comet assay (SSB) was observed. In the presence of the test chemicals the genotoxic effect was decreased to a level comparable to control values. In MCF-7 cells a significant increase in SSB level was observed after exposure to Ag15 at 16.3µg/mL. The effect was also diminished in the presence of the three test chemicals. In MDA-MB-231 cells no significant increase in SSB was observed, however increased level of oxidative DNA damage (incubation with Fpg enzyme) was observed after exposure to combinations of both AgNP with aluminium chloride. No increase in micronuclei formation was observed in neither cell line after the single nor combined treatments. Our results point to a low risk of increased genotoxic effects of AgNP when used in combination with aluminium salts, butylparaben or di-n-butylphthalate in consumer products.

Modeling emissions from CAFO poultry farms in Poland and evaluating potential risk to surrounding populations.

The world-wide use of concentrated animal feeding operations (CAFOs) for livestock production demands the need to evaluate the potential impact to public health. We estimated the exposure of various airborne pollutants for populations residing in close proximity to 10 poultry CAFOs located in Central Poland. Ammonia (NH3), carbon dioxide (CO2), carbon monoxide (CO), hydrogen sulfide (H2S), methane (CH4), nitrogen dioxide (NO2), nitrous oxide (N2O), sulfur dioxide (SO2), and organic dust were the pollutants of interest for this study. Because no monitoring data were available, we used the steady-state Gaussian dispersion model AERMOD to estimate pollutant concentrations for the exposed population in order to calculate the hazard index (HI) for a combined mixture of chemicals. Our results indicate that while the levels of certain pollutants are expected to exceed background levels commonly found in the environment they did not result in calculated hazard indexes which exceeded unity suggesting low potential for adverse health effects for the surrounding community for the mixture of chemicals. The study was conducted through a cooperation between the Agency for Toxic Substances and Disease Registry (ATSDR) in the USA and the Nofer Institute of Occupational Medicine (NIOM) in Poland.

[Internal control of working conditions as a form of occupational health and safety management--experience of Western European countries and the situation in Poland]. / Samokontrola warunków pracy jako forma zarzadzania bezpieczenstwem i zdrowiem pracujacych--doswiadczenia panstw zachodnioeuropejskich a sytuacja w Polsce.

Good occupational health and safety management not only protects workers from hazards and health impairment, but also contributes to the success of the enterprise as a whole. The internal control of working conditions in an enterprise, carried out by an employer, is one of the forms of occupational health and safety management implemented widely in many western countries, for example in Scandinavian countries and Ireland. This method may serve employers as an useful tool for stimulating and developing programmes aimed at improving working conditions and supporting workers' health protection against various hazards occurring in the work environment. The experience of the western countries in developing and implementing the internal control system, and the adaptation of the principles of this system to suit Polish conditions helped the authors to produce a set of general rules and guidelines addressed to employers who decide to use the method of internal control in their enterprises.