RESUMEN
Many studies implicate mitochondrial dysfunction as a key contributor to cell loss in Parkinson disease (PD). Previous analyses of dopaminergic (DAergic) neurons from patients with Lewy-body pathology revealed a deficiency in nuclear-encoded genes for mitochondrial respiration, many of which are targets for the transcription factor estrogen-related receptor gamma (Esrrg/ERRγ). We demonstrate that deletion of ERRγ from DAergic neurons in adult mice was sufficient to cause a levodopa-responsive PD-like phenotype with reductions in mitochondrial gene expression and number, that partial deficiency of ERRγ hastens synuclein-mediated toxicity, and that ERRγ overexpression reduces inclusion load and delays synuclein-mediated cell loss. While ERRγ deletion did not fully recapitulate the transcriptional alterations observed in postmortem tissue, it caused reductions in genes involved in synaptic and mitochondrial function and autophagy. Altogether, these experiments suggest that ERRγ-deficient mice could provide a model for understanding the regulation of transcription in DAergic neurons and that amplifying ERRγ-mediated transcriptional programs should be considered as a strategy to promote DAergic maintenance in PD.
RESUMEN
Deficiency in peroxisome proliferator-activated receptor gamma coactivator 1-alpha. (PGC-1α) expression or function is implicated in numerous neurological and psychiatric disorders. PGC-1α is required for the expression of genes involved in synchronous neurotransmitter release, axonal integrity, and metabolism, especially in parvalbumin-positive interneurons. As a transcriptional coactivator, PGC-1α requires transcription factors to specify cell-type-specific gene programs; while much is known about these factors in peripheral tissues, it is unclear if PGC-1α utilizes these same factors in neurons. Here, we identified putative transcription factors controlling PGC-1α-dependent gene expression in the brain using bioinformatics and then validated the role of the top candidate in a knockout mouse model. We transcriptionally profiled cells overexpressing PGC-1α and searched for over-represented binding motifs in the promoters of upregulated genes. Binding sites of the estrogen-related receptor (ERR) family of transcription factors were enriched, and blockade of ERRα attenuated PGC-1α-mediated induction of mitochondrial and synaptic genes in cell culture. Localization in the mouse brain revealed enrichment of ERRα expression in parvalbumin-expressing neurons with tight correlation of expression with PGC-1α across brain regions. In ERRα null mice, PGC-1α-dependent genes were reduced in multiple regions, including neocortex, hippocampus, and cerebellum, though not to the extent observed in PGC-1α null mice. Behavioral assessment revealed ambulatory hyperactivity in response to amphetamine and impairments in sensorimotor gating without the overt motor impairment characteristic of PGC-1α null mice. These data suggest that ERRα is required for normal levels of expression of PGC-1α-dependent genes in neurons but that additional factors may be involved in their regulation.
Asunto(s)
Encéfalo , Receptores de Estrógenos , Animales , Encéfalo/metabolismo , Expresión Génica , Regulación de la Expresión Génica , Ratones , Ratones Noqueados , Coactivador 1-alfa del Receptor Activado por Proliferadores de Peroxisomas gamma/genética , Receptores de Estrógenos/genética , Receptores de Estrógenos/metabolismo , Factores de Transcripción , Receptor Relacionado con Estrógeno ERRalfaRESUMEN
Transcripts derived from the thyroid hormone receptor alpha (TRalpha) gene are alternatively spliced resulting in a functional receptor TRalpha1 and a non-T3-binding variant TRalpha2 that can exert a dominant negative effect on the transactivation functions of other TRs. There is evidence that the ratio of TRalpha isoform transcripts can be modulated and here, we investigate whether the PPARgamma co-activator alpha (PGC-1alpha) has an effect on this splicing process. PGC-1alpha was discovered not only as a transcriptional co-activator, but also has certain motifs characteristic of splicing factors. We demonstrate that PGC-1alpha alters the ratio of endogenously expressed TRalpha isoform transcripts in HepG2 cells, by decreasing TRalpha1 mRNA levels twofold. This change in isoform ratio is accompanied by a decrease in 5'-deiodinase expression, whereas no differences were found in TRbeta1 expression. Deletion of the RNA-processing domain of PGC-1alpha abrogated the effect on the TRalpha splicing, whereas expression of only the RNA-processing domain favored TRalpha1 expression. PGC-1alpha showed a similar effect on the splicing of a TRalpha minigene containing only the last four exons and introns of the TRalpha gene. These data suggest that PGC-1alpha is involved in the RNA processing of TRalpha transcripts.
Asunto(s)
Empalme Alternativo/fisiología , Regulación de la Expresión Génica , Proteínas de Choque Térmico/fisiología , Procesamiento Postranscripcional del ARN/fisiología , Receptores alfa de Hormona Tiroidea/metabolismo , Factores de Transcripción/fisiología , Animales , Eliminación de Gen , Perfilación de la Expresión Génica , Proteínas de Choque Térmico/metabolismo , Humanos , Proteínas Mutantes/fisiología , Coactivador 1-alfa del Receptor Activado por Proliferadores de Peroxisomas gamma , Isoformas de Proteínas/metabolismo , ARN Mensajero/metabolismo , Ratas , Factores de Transcripción/metabolismo , Triyodotironina/farmacología , Células Tumorales CultivadasRESUMEN
PGC-1 was originally identified as a transcriptional coactivator of the nuclear receptor PPARgamma. The expression pattern and induction by exposure to cold have implicated PGC-1 in the regulation of energy metabolism and adaptive thermogenesis. Remarkably, PGC-1 overexpression can induce mitochondrial biogenesis and functions. Recent studies show that PGC-1 regulates the activity of several nuclear receptors and other transcription factors, and thus acts in a broader context than previously anticipated. Furthermore, PGC-1 displays the striking ability to interact with components of the splicing machinery. PGC-1 could therefore allow coordinated regulation of transcription and splicing in response to signals relaying metabolic needs. These novel findings are discussed in the context of the proposed physiological functions of PGC-1.
Asunto(s)
Factores de Transcripción/fisiología , Animales , Proteínas de Unión al ADN , Metabolismo Energético , Regulación de la Expresión Génica , Empalme del ARN , Receptores Citoplasmáticos y Nucleares/fisiología , Termogénesis , Factores de Transcripción/genéticaRESUMEN
Mechanisms and signals that regulate transcriptional coactivators are still largely unknown. Here we provide genetic evidence for a repressor that interacts with and regulates the nuclear receptor coactivator PGC-1. Association with the repressor requires a PGC-1 protein interface that is similar to the one used by nuclear receptors. Removal of the repressor enhances PGC-1 coactivation of steroid hormone responses. We also provide evidence that interaction of the repressor with PGC-1 is regulated by mitogen-activated protein kinase (MAPK) signaling. Activation of the MAPK p38 enhances the activity of wild-type PGC-1 but not of a PGC-1 variant that no longer interacts with the repressor. Finally, p38 activation enhances steroid hormone response in a PGC-1-dependent manner. Our data suggest a model where the repressor and nuclear receptors compete for recruiting PGC-1 to an inactive and active state, respectively. Extracellular signals such as nuclear receptor ligands or activators of the MAPK p38 can shift the equilibrium between the two states.
Asunto(s)
Regulación de la Expresión Génica/fisiología , Sistema de Señalización de MAP Quinasas/fisiología , Proteínas Quinasas Activadas por Mitógenos/metabolismo , Proteínas Represoras/metabolismo , Factores de Transcripción/metabolismo , Secuencias de Aminoácidos , Animales , Unión Competitiva , Células COS , Chlorocebus aethiops , Activación Enzimática , Glucocorticoides/farmacología , Células HeLa , Humanos , Sustancias Macromoleculares , Modelos Biológicos , Receptor Cross-Talk , Receptores de Glucocorticoides/efectos de los fármacos , Receptores de Glucocorticoides/fisiología , Proteínas Recombinantes de Fusión/biosíntesis , Saccharomyces cerevisiae/genética , Factores de Transcripción/genética , Transcripción Genética , Transfección , Técnicas del Sistema de Dos Híbridos , Proteínas Quinasas p38 Activadas por MitógenosRESUMEN
To find novel components in the glucocorticoid signal transduction pathway, we performed a yeast genetic screen to identify ligand-effect modulators (LEMs), proteins that modulate the cellular response to hormone. We isolated several mutants that conferred increased glucocorticoid receptor (GR) activity in response to dexamethasone and analyzed two of them in detail. These studies identify two genes, LEM3 and LEM4, which correspond to YNL323w and ERG6, respectively. LEM3 is a putative transmembrane protein of unknown function, and ERG6 is a methyltransferase in the ergosterol biosynthetic pathway. Analysis of null mutants indicates that LEM3 and ERG6 act at different steps in the GR signal transduction pathway.
Asunto(s)
Dexametasona/farmacología , Receptores de Glucocorticoides/genética , Proteínas de Saccharomyces cerevisiae , Saccharomyces cerevisiae/genética , Transducción de Señal/fisiología , Transportadoras de Casetes de Unión a ATP/genética , Animales , Secuencia de Bases , Canavanina/farmacología , Clonación Molecular , Cruzamientos Genéticos , Cartilla de ADN , Proteínas Fúngicas/genética , Proteínas Fúngicas/metabolismo , Prueba de Complementación Genética , Proteínas de la Membrana/genética , Proteínas de la Membrana/metabolismo , Metiltransferasas/genética , Metiltransferasas/metabolismo , Datos de Secuencia Molecular , Mutagénesis , Reacción en Cadena de la Polimerasa , Receptores de Glucocorticoides/efectos de los fármacos , Receptores de Glucocorticoides/fisiología , Proteínas Recombinantes/efectos de los fármacos , Proteínas Recombinantes/metabolismo , Saccharomyces cerevisiae/efectos de los fármacos , Saccharomyces cerevisiae/fisiología , Rayos UltravioletaRESUMEN
Steroid receptors mediate responses to lipophilic hormones in a tissue- and ligand-specific manner. To identify nonreceptor proteins that confer specificity or regulate steroid signaling, we screened a human cDNA library in a steroid-responsive yeast strain. One of the identified cDNAs, isolated in the screen as ligand effect modulator 6, showed no homology to yeast or Caenorhabditis elegans proteins but high similarity to the recently described mouse coactivator PGC-1 and was accordingly termed hPGC-1. The hPGC-1 DNA encodes a nuclear protein that is expressed in a tissue-specific manner and carries novel motifs for transcriptional regulators. The expression of hPGC-1 in mammalian cells enhanced potently the transcriptional response to several steroids in a receptor-specific manner. hPGC-1-mediated enhancement required the receptor hormone-binding domain and was dependent on agonist ligands. Functional analysis of hPGC-1 revealed two domains that interact with steroid receptors in a hormone-dependent manner, a potent transcriptional activation function, and a putative dimerization domain. Our findings suggest a regulatory function for hPGC-1 as a tissue-specific coactivator for a subset of nuclear receptors.
Asunto(s)
Pruebas Genéticas , Proteínas Nucleares/genética , Receptores de Esteroides/metabolismo , Animales , Sitios de Unión/genética , Células COS , Clonación Molecular , Dimerización , Regulación de la Expresión Génica , Humanos , Microscopía Fluorescente , Proteínas Nucleares/metabolismo , Receptores de Glucocorticoides/metabolismo , Esteroides/farmacología , Factores de Transcripción/metabolismo , Activación Transcripcional , Transfección , LevadurasRESUMEN
Drug resistance of pathogens is an increasing problem whose underlying mechanisms are not fully understood. Cellular uptake of the major drugs against Trypanosoma brucei spp., the causative agents of sleeping sickness, is thought to occur through an unusual, so far unidentified adenosine transporter. Saccharomyces cerevisiae was used in a functional screen to clone a gene (TbAT1) from Trypanosoma brucei brucei that encodes a nucleoside transporter. When expressed in yeast, TbAT1 enabled adenosine uptake and conferred susceptibility to melaminophenyl arsenicals. Drug-resistant trypanosomes harbor a defective TbAT1 variant. The molecular identification of the entry route of trypanocides opens the way to approaches for diagnosis and treatment of drug-resistant sleeping sickness.
Asunto(s)
Adenosina/metabolismo , Proteínas Portadoras/metabolismo , Proteínas de la Membrana/metabolismo , Tripanocidas/farmacología , Trypanosoma brucei brucei/efectos de los fármacos , Trypanosoma brucei brucei/metabolismo , Secuencia de Aminoácidos , Animales , Arsenicales/metabolismo , Arsenicales/farmacología , Transporte Biológico , Proteínas Portadoras/química , Proteínas Portadoras/genética , Clonación Molecular , Resistencia a Medicamentos/genética , Genes Protozoarios , Proteínas de la Membrana/química , Proteínas de la Membrana/genética , Datos de Secuencia Molecular , Mutación , Proteínas de Transporte de Nucleósidos , Nucleósidos/metabolismo , Purinas/metabolismo , Purinas/farmacología , Saccharomyces cerevisiae/genética , Especificidad por Sustrato , Tripanocidas/metabolismo , Trypanosoma brucei brucei/genética , Tripanosomiasis Africana/tratamiento farmacológico , Tripanosomiasis Africana/parasitologíaRESUMEN
Overexpression of the yeast Pdr5 ATP-binding cassette transporter leads to pleiotropic drug resistance to a variety of structurally unrelated cytotoxic compounds. To identify Pdr5 residues involved in substrate recognition and/or drug transport, we used a combination of random in vitro mutagenesis and phenotypic screening to isolate novel mutant Pdr5 transporters with altered substrate specificity. A plasmid library containing randomly mutagenized PDR5 genes was transformed into appropriate drug-sensitive yeast cells followed by phenotypic selection of Pdr5 mutants. Selected mutant Pdr5 transporters were analyzed with respect to their expression levels, subcellular localization, drug resistance profiles to cycloheximide, rhodamines, antifungal azoles, steroids, and sensitivity to the inhibitor FK506. DNA sequencing of six PDR5 mutant genes identified amino acids important for substrate recognition, drug transport, and specific inhibition of the Pdr5 transporter. Mutations were found in each nucleotide-binding domain, the transmembrane domain 10, and, most surprisingly, even in predicted extracellular hydrophilic loops. At least some point mutations identified appear to influence folding of Pdr5, suggesting that the folded structure is a major substrate specificity determinant. Surprisingly, a S1360F exchange in transmembrane domain 10 not only caused limited substrate specificity, but also abolished Pdr5 susceptibility to inhibition by the immunosuppressant FK506. This is the first report of a mutation in a yeast ATP-binding cassette transporter that allows for the functional separation of substrate transport and inhibitor susceptibility.
Asunto(s)
Transportadoras de Casetes de Unión a ATP/genética , Farmacorresistencia Microbiana/genética , Resistencia a Múltiples Medicamentos/genética , Proteínas de la Membrana/genética , Proteínas de Saccharomyces cerevisiae , Saccharomyces cerevisiae/efectos de los fármacos , Tacrolimus/farmacología , Transportadoras de Casetes de Unión a ATP/antagonistas & inhibidores , Transportadoras de Casetes de Unión a ATP/química , Secuencia de Aminoácidos , Sustitución de Aminoácidos , Antifúngicos/farmacología , Transporte Biológico , Proteínas Portadoras/genética , Proteínas Portadoras/fisiología , Membrana Celular/química , Clonación Molecular , Cicloheximida/farmacología , Proteínas de Unión al ADN/genética , Proteínas de Unión al ADN/fisiología , Dexametasona/metabolismo , Dexametasona/farmacología , Estradiol/metabolismo , Expresión Génica/efectos de los fármacos , Proteínas de Choque Térmico/genética , Proteínas de Choque Térmico/fisiología , Proteínas de la Membrana/antagonistas & inhibidores , Proteínas de la Membrana/química , Datos de Secuencia Molecular , Mutagénesis , Rodamina 123 , Rodaminas/metabolismo , Rodaminas/farmacología , Saccharomyces cerevisiae/genética , Alineación de Secuencia , Especificidad por Sustrato , Proteínas de Unión a TacrolimusRESUMEN
Steroid hormones bind and activate intracellular receptors that are ligand-regulated transcription factors. Mammalian steroid receptors can confer hormone-dependent transcriptional enhancement when expressed in yeast, thereby enabling the genetic identification of nonreceptor proteins that function in the hormone signal transduction pathway. Pdr5p (Lem1/Sts1/Ydr1p), a yeast ATP-binding cassette transporter, selectively decreases the intracellular levels of particular steroid hormones, indicating that active processes can affect the passage of steroids across biological membranes. In yeast, the immunosuppressive drug FK506 inhibited Pdr5p, thereby potentiating activation of the glucocorticoid receptor by dexamethasone, a ligand that is exported by Pdr5p. In mammalian L929 cells but not in HeLa cells, FK506 potentiated dexamethasone responsiveness and increased dexamethasone accumulation, without altering the hormone-binding properties of the glucocorticoid receptor. We suggest that an FK506-sensitive transporter in L929 cells selectively decreases intracellular hormone levels and, consequently, the potency of particular steroids. Thus, steroid transporters may modulate, in a cell-specific manner, an initial step in signaling, the availability of hormone to the receptor.
Asunto(s)
Transportadoras de Casetes de Unión a ATP/metabolismo , Desoxicorticosterona/farmacología , Dexametasona/farmacología , Proteínas de la Membrana/metabolismo , Receptores de Glucocorticoides/fisiología , Proteínas de Saccharomyces cerevisiae , Saccharomyces cerevisiae/fisiología , Tacrolimus/farmacología , Animales , Proteínas Fúngicas/metabolismo , Células HeLa , Humanos , Células L , Mamíferos , Ratones , Receptores de Glucocorticoides/biosíntesis , Proteínas Recombinantes/metabolismo , Saccharomyces cerevisiae/efectos de los fármacos , Transducción de Señal , Transfección , Triamcinolona Acetonida/farmacologíaAsunto(s)
Proteínas Fúngicas/fisiología , Proteínas de Choque Térmico , Chaperonas Moleculares/fisiología , Receptores de Esteroides/metabolismo , Saccharomyces cerevisiae/metabolismo , Transducción de Señal , Proteínas Fúngicas/genética , Proteínas del Choque Térmico HSP40 , Proteínas HSP70 de Choque Térmico/fisiología , Proteínas HSP90 de Choque Térmico/fisiología , Mutación , Proteína Oncogénica pp60(v-src)/metabolismo , Pliegue de Proteína , Receptores de Estrógenos/metabolismo , Receptores de Glucocorticoides/metabolismo , Saccharomyces cerevisiae/genética , Proteínas de Saccharomyces cerevisiaeRESUMEN
The rat glucocorticoid receptor confers hormone-dependent transcriptional enhancement when expressed in yeast, thereby enabling the genetic identification of nonreceptor proteins that function in the hormone signal-transduction pathway. We isolated a yeast mutant, lem1, with increased sensitivity to dexamethasone and triamcinolone acetonide; responsiveness to a third agonist, deoxycorticosterone, is unaffected. Cloning of wild-type LEM1 revealed a putative transport protein of the ATP-binding cassette family. Dexamethasone accumulation is increased in lem1 cells, suggesting that wild-type LEM1 decreases dexamethasone potency by exporting this ligand. LEM1 appears to affect certain steroids and not others. We propose that transporters like LEM1 can selectively modulate the intracellular levels of steroid hormones. Differential activities of such transporters in mammalian cells might regulate hormone availability and thereby hormone signaling in a cell-type specific manner.
Asunto(s)
Transportadoras de Casetes de Unión a ATP/metabolismo , Proteínas Portadoras/metabolismo , Dexametasona/farmacología , Receptores de Esteroides/metabolismo , Proteínas de Saccharomyces cerevisiae , Saccharomyces cerevisiae/metabolismo , Transportadoras de Casetes de Unión a ATP/biosíntesis , Adenosina Trifosfato/metabolismo , Animales , Clonación Molecular , Desoxicorticosterona/farmacología , Dexametasona/metabolismo , Estradiol/metabolismo , Metanosulfonato de Etilo/farmacología , Prueba de Complementación Genética , Cinética , Mamíferos , Mutagénesis , Ratas , Receptores de Estrógenos/metabolismo , Receptores de Glucocorticoides/metabolismo , Receptores de Esteroides/efectos de los fármacos , Proteínas Recombinantes/metabolismo , Saccharomyces cerevisiae/efectos de los fármacos , Saccharomyces cerevisiae/genética , Triamcinolona Acetonida/farmacologíaRESUMEN
Tumorigenesis in mice of the rat insulin promoter [RIP]-simian virus 40 tumor antigen [SV40 Tag] transgenic lineages, RIP1-Tag2 and RIP1-Tag4, is a process initiated by expression of SV40 Tag in pancreatic beta cells, evolution of islet cell hyperplasia and insulinoma appearance. Analysis of major histocompatibility complex [MHC] class I gene expression during this process revealed a normal level of MHC class I molecules at the surface of pancreatic islet cells of RIP1-Tag4 mice, while hyperplastic islets from the same mice contained cells expressing a normal level and cells expressing a low level of MHC class I antigen. Insulinomas themselves expressed very low levels or no MHC class I gene product. Thus, down-regulation of MHC class I gene appears to accompany tumor progression of SV40 Tag-transformed beta islet cells. MHC class I antigen expression in a series of clonally derived cell lines of beta cell origin from different SV40 Tag-induced insulinomas ranged from quite low to undetectable, although expression was inducible by interferon-gamma. Nuclear run-on and transient transfection analyses indicated that expression of the MHC class I gene in these cells in controlled at the transcriptional level, and that the decreased expression is paralleled by reduced binding of transcription factors to the R1 element of the H-2 enhancer.
Asunto(s)
Regulación hacia Abajo , Elementos de Facilitación Genéticos , Antígenos H-2/genética , Antígenos de Histocompatibilidad Clase I/metabolismo , Insulinoma/metabolismo , Islotes Pancreáticos/metabolismo , Animales , Secuencia de Bases , Northern Blotting , Línea Celular , Línea Celular Transformada , Cloranfenicol O-Acetiltransferasa/genética , Cloranfenicol O-Acetiltransferasa/metabolismo , Interferón gamma/farmacología , Ratones , Ratones Endogámicos C57BL , Datos de Secuencia Molecular , Transfección , Células Tumorales CultivadasRESUMEN
In transformed cells, the E1A gene of adenovirus type 12 (Ad12) represses transcription of class I genes of the major histocompatibility complex. The tumorigenic potential of Ad12-transformed cells correlates with this diminished class I expression. In contrast, the E1A gene of the nontumorigenic Ad5 does not affect class I expression. We show here that a transfected reporter chloramphenicol acetyltransferase plasmid driven by an H-2K promoter (-1049 bp) was expressed at much lower levels in Ad12- than in Ad5-transformed mouse cells. Analysis of mutant constructs revealed that only 83 bp of H-2 DNA, consisting of the enhancer juxtaposed to the basal promoter, was sufficient for this differential expression. Whereas the H-2 basal promoter alone was somewhat less active in Ad12-transformed cells, the H-2 TATA box itself did not appear to be important. The H-2 enhancer proved to be the principal element in Ad12 E1A-mediated repression, since (i) substitution of the H-2 enhancer by simian virus 40 enhancers overcame the repression, and (ii) when juxtaposed to either its native or heterologous basal promoters, the H-2 enhancer was functional in Ad5- but not Ad12-transformed cells. Mobility shift assays showed that there is a DNA-binding activity to the 5' site (R2 element) of the enhancer that is significantly higher in Ad12- than in Ad5-transformed cells. These results suggest that decreased class I enhancer activity in Ad12-transformed cells may, at least in part, be due to the higher levels of an enhancer-specific factor, possibly acting as a repressor.
Asunto(s)
Proteínas E1A de Adenovirus/genética , Adenovirus Humanos/genética , Transformación Celular Neoplásica , Elementos de Facilitación Genéticos , Regulación Viral de la Expresión Génica , Genes MHC Clase I , Genes Virales , Antígenos H-2/genética , TATA Box , Transcripción Genética , Animales , Secuencia de Bases , Línea Celular Transformada , Cloranfenicol O-Acetiltransferasa/genética , Cloranfenicol O-Acetiltransferasa/metabolismo , Ratones , Ratones Endogámicos BALB C , Datos de Secuencia Molecular , Mutagénesis Sitio-Dirigida , Oligodesoxirribonucleótidos , Regiones Promotoras Genéticas , Proteínas Recombinantes/metabolismoRESUMEN
In cells transformed by the highly oncogenic adenovirus type 12 (Ad12), the viral E1A proteins mediate transcriptional repression of the major histocompatibility class I genes. In contrast, class I transcription is not reduced in cells transformed by the nononcogenic Ad5. The decreased rate of class I transcription is, at least in part, the result of a reduced major histocompatibility complex class I enhancer activity in Ad12-transformed cells and correlates with an increase in the levels of a DNA-binding activity to the R2 element of the enhancer (R. Ge, A. Kralli, R. Weinmann, and R. P. Ricciardi, J. Virol. 66:6969-6978, 1992). Employing transient transfection assays, we now provide direct evidence that the R2 element can confer repression in Ad12- but not Ad5-transformed cells. Repression by R2 was observed only in the presence of the positive enhancer element R1 and was dependent on (i) the number of the R2 elements and (ii) the relative arrangement of R2 and R1 elements. The putative R2-binding repressor protein, R2BF, was similar in molecular weight and binding specificity to members of the thyroid hormone/retinoic acid (RA) receptor family. RA treatment abrogated the R2-mediated repression in Ad12-transformed cells and had no effect on the activity of R2/R1-containing promoters in Ad5-transformed cells. These results are consistent with the presence of an R2-binding repressor in Ad12-transformed cells. In the absence of RA, the repressor compromises enhancer activity by interfering with the activity of the positive cis element R1. RA treatment of Ad12-transformed cells may render the repressor inactive.
Asunto(s)
Adenovirus Humanos/genética , Transformación Celular Neoplásica , Elementos de Facilitación Genéticos , Regulación Viral de la Expresión Génica , Genes MHC Clase I , Antígenos H-2/genética , Regiones Promotoras Genéticas , Transcripción Genética , Tretinoina/farmacología , Proteínas E1A de Adenovirus/genética , Animales , Secuencia de Bases , Sitios de Unión , Línea Celular Transformada , Núcleo Celular/metabolismo , Cloranfenicol O-Acetiltransferasa/genética , Cloranfenicol O-Acetiltransferasa/metabolismo , Regulación Viral de la Expresión Génica/efectos de los fármacos , Ratones , Ratones Endogámicos BALB C , Datos de Secuencia Molecular , Proteínas Recombinantes/metabolismo , TransfecciónRESUMEN
Adenovirus E1A has long been known to activate/repress cellular and viral transcription. The transcriptional activity of nuclear extracts was depleted after chromatography on immobilized E1A protein columns that specifically retained the transcription factor (TF) IID. Stronger direct interactions between E1A and human TFIID than between E1A and yeast TFIID suggest that the unique sequences of the human protein may be involved. We have demonstrated that this interaction occurs directly between bacterially produced E1A and bacterially produced human TFIID in a protein blot assay. We propose that E1A protein may transduce regulatory signals from upstream activators to basal elements of the transcriptional machinery by contacting TFIID.
Asunto(s)
Proteínas Oncogénicas Virales/metabolismo , TATA Box , Factores de Transcripción/metabolismo , Proteínas Precoces de Adenovirus , Antígenos Virales de Tumores/inmunología , Western Blotting , Cromatografía de Afinidad , Electroforesis en Gel de Poliacrilamida , Escherichia coli/genética , Regulación Bacteriana de la Expresión Génica , Genes Bacterianos , Humanos , Saccharomyces cerevisiae/metabolismo , Factor de Transcripción TFIID , Transcripción GenéticaRESUMEN
Near-UV radiation (365 nm)-induced lethality, as measured by colony-forming ability, showed an actinic reticuloid cell strain to be sensitive relative to normal human fibroblasts, when irradiated at 25 degrees C. This effect was not seen after far-UV (254 nm) irradiation. Trolox-C, a water-soluble analogue of vitamin E, incorporated in the pre-irradiation growth medium or in the post-irradiation plating medium, protected the actinic reticuloid cells to the extent that they were as resistant as normal cells. Plating medium containing Trolox-C did not provide differential protection against inactivation of the two cell strains by wavelengths in the far-UV region. The protection provided by Trolox-C, an analogue of the natural antioxidant vitamin E, suggests some free radical involvement in the aetiology of the disease.