RESUMEN
Immunomodulatory properties of S. pyogenes protein M111 were studied on the model of Gurov strain and its isogenic mutant not expressing M protein. Mouse resident peritoneal macrophages were incubated with bacteria and generation of nitroxide and superoxide anions and production of IL-6, IL-10, and IL-17 were evaluated. Protein M111 modified macrophage response: it exhibited antiphagocytic activity, prevented ROS formation, and stimulated the production of anti-inflammatory cytokine IL-10. The results suggested that this protein could serve in the bacteria as a factor suppressing the host defense forces and promoting the realization of the strategy beneficial for pathogens - escape from the host immune defense.
Asunto(s)
Antígenos Bacterianos/genética , Proteínas de la Membrana Bacteriana Externa/genética , Proteínas Portadoras/genética , Eliminación de Gen , Evasión Inmune , Macrófagos Peritoneales/microbiología , Streptococcus pyogenes/genética , Animales , Antígenos Bacterianos/inmunología , Proteínas de la Membrana Bacteriana Externa/inmunología , Proteínas Portadoras/inmunología , Expresión Génica , Interleucina-10/biosíntesis , Interleucina-10/inmunología , Interleucina-17/biosíntesis , Interleucina-17/inmunología , Interleucina-6/biosíntesis , Interleucina-6/inmunología , Macrófagos Peritoneales/inmunología , Ratones , Ratones Endogámicos C57BL , Ratones Endogámicos CBA , Óxidos de Nitrógeno/inmunología , Óxidos de Nitrógeno/metabolismo , Fagocitosis , Cultivo Primario de Células , Streptococcus pyogenes/inmunología , Streptococcus pyogenes/patogenicidad , Superóxidos/inmunología , Superóxidos/metabolismoAsunto(s)
Anticuerpos Antivirales/biosíntesis , Inmunoglobulina G/biosíntesis , Virus de la Influenza A , Infecciones por Orthomyxoviridae/complicaciones , Infecciones por Orthomyxoviridae/inmunología , Infecciones Estreptocócicas/complicaciones , Infecciones Estreptocócicas/inmunología , Streptococcus agalactiae , Animales , Anticuerpos Antivirales/sangre , Células Productoras de Anticuerpos/inmunología , Inmunoglobulina G/sangre , Virus de la Influenza A/inmunología , Ratones , Ratones Endogámicos C57BL , Ratones Endogámicos CBA , Bazo/inmunología , Factores de TiempoRESUMEN
Coxiella burnetii antigens stimulate the defence against growth of hepatoma 22a cells. The antigen-stimulated mice survived longer, they considerably later developed palpable tumours and showed a retarded tumour growth. The enhanced resistance to tumour growth may be explained by at least 2 interrelated phenomena; namely by the induction of interferon-like activity and an increased NK cell activity.
Asunto(s)
Adyuvantes Inmunológicos , Antígenos Bacterianos/administración & dosificación , Coxiella/inmunología , Neoplasias Hepáticas Experimentales/terapia , Animales , Inmunoterapia , Interferones/biosíntesis , Células Asesinas Naturales/inmunología , Neoplasias Hepáticas Experimentales/inmunología , Ratones , Ratones Endogámicos C3HRESUMEN
The effect of immobilization stress on the course of various forms of influenza infection has been investigated. Influenza was produced in 10-14-week-old inbred mice by intranasal infection with pathogenic influenza virus strain A/PR/8/34 (H1N1) at different doses. Immobilization for 6 hr resulted in the appearance of virus-inhibiting activity in the serum of mice. This activity suppressed the reproduction of test-virus in tissue culture, it was resistant to acid pH 2.0 treatment and to heating at 56 degrees C. However, the high level of virus-inhibiting activity failed to protect the animals from subsequent development of lethal influenza infection. Immobilization stress caused a transient depression of virus induced interferon (IFN) production, as revealed by the use of virus inducer at early intervals after stress. Contemporarily, the stress could aggravate the course of virus infection promoting its transition from non-lethal form into a lethal one and virus penetration into brain.
