Hydroxyapatite-oil composites for delivering AZT in simulated body fluid.

Hydroxyapatite (HA) has been used to deliver therapeutic drugs both in vitro and in vivo at a constant rate showing zero order kinetics. This study was designed specifically to analyze the effects of wheat germ (WG) incorporation with HA on the rate of delivery of AZT from an insert system over a one month period in vitro. Insert systems which were saturated with wheat germ oil delivered AZT at a slower rate over the one month period than did half-saturated or unsaturated insert systems. All systems containing 50 mg of AZT in the outer shell delivered 80% of the 100 mg AZT dosage over the first eight days. The systems which had a 100 mg AZT insert surrounded by an oily HA shell lacking AZT delivered AZT in a linear manner over the course of one month. The amounts and rates of AZT release from composites was indirectly proportional to the amount of wheat germ oil used. The results of this study show that the lipids incorporated in the ceramic composites can be tailored to deliver a 100 mg AZT dosage for a period of one month in vitro.

Parameters of protein delivery using hydroxyapatite in simulated body fluid.

Various studies have been conducted using hydroxyapatite (HA) to deliver therapeutic drugs over a long period of time. However, the rate of drug release from ceramics varies tremendously. Thus a study was designed to observe the effect of particle size, pressure, drug ratio, and the addition of a zinc stearate binder on the release of BSA from ceramics. Samples were collected every two hours for a 12 hour period. Three particle sizes were used in the study (< 38, 45-63, and 63-75 microns). Variations in particle size did not influence the release of BSA. Ceramics compressed at a pressure of 150 Mpa delivered more protein than pressures of 300 MPa, 450 MPa, and 900 MPa. Drug to ceramic ratio had the most significant effect. A ratio of 1:25 BSA to HA delivered the protein quickly whereas the 1:100 BSA to HA delivered BSA to HA delivered BSA slowly and in zero order kinetics. The addition of the zinc binder improved the quality of the composite and decreased the release rate of protein delivery when present in 5% or less of the total ceramic weight. HA ceramics can be used to deliver proteins at different rates by varying compression pressure and drug to HA ratio.