[The multiplex method of estimation of humoral immunity to vaccine regulated childhood infections].

The goal of this work was to present the results of the laboratory tests of the multiplex dot immunoassay method using protein microarray for complex estimation of humoral immunity to measles, mumps, and rubella viruses. It was shown that the obtained results were in a good agreement with data of commercial monospecific ELISA kits. The developed method is fast, requires fewer resources, and may be used in the field.

[Multiprofile immunochemical indication of the causative agents in infectious diseases].

A variant of multiprofile immunochemical indication of the causative agents of infectious diseases is described on the basis of a protein microarray with a low-density antibody population. The method is a multiplex variant of dot ELISA whose sensitivity increased due to the use of a colloid gold-based conjugate and an efficient system of its development. The method shows both its multiprofile character with its easiness-to-prepare and to use protein microchips. Having the similar sensitivity, it has obvious advantages over the routine ELISA in the rate of obtaining results, the consumption of reagents, labor intensiveness, and specific cost.

[Dyspepsia and gastroesophageal reflux in adolescents]. / Dispepsiia i gastroézofageal'nyi refliuks u podrostkov.

AIM: To study the prevalence and risk factors for dyspepsia and gastroesophageal reflux (GER) among adolescents. MATERIAL AND METHODS: Senior schoolchildren (forms 9-11) of four randomly selected secondary schools of Novosibirsk participated in the study (189 boys and 260 girls aged 14-17 years). They filled in the Bowel Disease Questionnaire, and sera were tested for antibodies against Helicobacter pylori infection. RESULTS: Dyspepsia was reported by 21% of the schoolchildren, being more frequent in girls (25%) than boys (14%, p = 0.004). GER occurred in 22% of adolescents with the same frequency in both genders. The combination of dyspepsia with GER was found in 8%. The prevalence of H. pylori infection was 56%. No association was found between the infection and abdominal symptoms, except heartburn. Among factors associated with dyspepsia were female gender, irregular meals, family history of dyspepsia. GER was related to H. pylori infection, obesity, smoking and irregular meals, 46% of adolescents with dyspepsia and 36% of those with GER have consulted a physician, and school absenteeism was reported by 55 and 40%, respectively. CONCLUSION: Dyspepsia and gastroesophageal reflux are widespread among the adolescent population and result in frequent use of health care resources.

[Prevalence and risk factors of the development of irritable bowel syndrome in adolescents: a population study]. / Rasprostranennost' i faktory riska razvitiia sindroma razdrazhennogo kishechnika u podrostkov: populiatsionnoe issledovanie.

AIM: To determine prevalence of irritable bowel syndrome (IBS) and associated factors in a community-based population of adolescents. MATERIAL AND METHODS: 189 schoolboys and 250 schoolgirls aged 14-17 years from four randomly chosen schools of Novosibirsk participated in the study. RESULTS: The prevalence of IBS defined according to Rome criteria was higher in girls (24 vs 14% in boys, p = 0.01). Colonic pain frequency was similar in both genders (11% in girls vs 9% in boys, p = 0.46), however disturbed defecation occurred significantly more often in girls than in boys (16 vs 7%, p = 0.003). Half of the subjects who reported IBS had also dyspeptic symptoms. Fiber-rich diet and/or laxatives to prevent constipation were used by 2% of boys and 7% of girls (p = 0.012). However, only one third of those having less than 3 stools a week thought it abnormal. IBS is associated with the age over 15-16 years, female gender, abdominal pain in childhood, appendectomy, irregular meals and smoking (in girls). No association was found with other socio-demographic factors and serological markers of some infections and invasions (H. pylori, G. lamblia, O. felineus, hepatitis viruses B and C). Of the persons with diagnosable IBS, 49% have consulted a physician about it, 21% have undergone upper endoscopy. School absenteeism was reported by 62% of the IBS sufferers. CONCLUSION: IBS is common among adolescents (especially girls) with the prevalence rates similar to those described in adults from West Europe and USA. These disorders are associated with significant disability and health care costs.

[Serodiagnosis of chlamydiosis: "Chlamy-IgG-DS-Tr"-- the first domestic immunoenzyme recombinant test-system for determination of anti-Chlamydia trachomatis class G antibodies]. / Serodiagnostika khlamidiozov. "Khlami-IgG-DS-Tr"--pervaia otechestvennaia immunofermentnaia rekombinantnaia test-sistema dlia vyiavleniia antitel klassa G k Chlamydia trachomatis.

The first Native test-system for determination of class G antibodies to Chlamydia trachomatis with chlamydia recombinant antigen was elaborated Test-system efficacy was demonstrated in clinical trials. The sensibility, specific activity and suitability of the "Chlamy-IgG-DS-Tr" were the same as for import analogous.

[Relationship between the level of specific antibodies with disease outcome in Cercopithecus aethiops monkeys in experimental Marburg disease]. / O vzaimosviazi urovnia spetsificheskikh antitel s iskhodom zabolevaniiau obez'ian Cerpithecus aethiops pri éksperimental'noi infektsii virus Marburg.

The level of specific antibodies in the blood of Cercopithecus aethiops monkeys with experimental Marburg hemorrhagic fever appreciably affects the incidence of lethal outcomes in immunized animals infected with Marburg virus. This effect manifests starting from the titers as low as just 1:100. The level of humoral immunity with the specific antibody titer of at least 1:100 depends on the method of antigen preparation. Humoral response was higher upon challenge with formalin-inactivated virus isolated from the blood plasma of infected guinea pigs.

[Changes in the blood serum aminotransferase activity in the experimental infection of Cercopithecus aethiops monkeys with the Marburg virus]. / Izmeneniia aktivnosti aminotransferaz syvorotki krovi pri éksperimental'nom zarazhenii obez'ian Cercopithecus aethiops virusom Marburg.

Aminotransferase levels in the peripheral blood serum of African green monkeys were studied after aerosol infection with lyophilized virus preparation. A correlation between aminotransferase activity and other symptoms of hemorrhagic fever was shown. Considerable variations in the features of aminotransferase activity in severe and mild cases of the disease were observed.

[The effect of the methods for producing an experimental Marburg virus infection on the characteristics of the course of the disease in green monkeys]. / Vliianie sposobov éksperimental'nogo zarazheniia virusom Marburg na osobennosti protekaniia bolezni u zelenykh martyshek.

A comparative study on the features of the pathogenesis of Marburg disease after parenteral and aerosol infection of green monkeys with a virus prepared from native culture suspension and that after lyophilization was carried out. The changes in the dynamics of the clotting time, the activity of serum aminotransferases, the duration of prefebrile period and survival time were analysed in different cases. No lethality was observed in animals infected with small doses of aerosol preparations.