Impact of lead and zinc heavy metal pollution on the growth and phytoremediation potential of Sulla carnosa in Sebkha el Kalbia, Tunisia.

Heavy metal pollution from human and natural activities poses significant environmental and health concerns for wildlife and humans, with lead and zinc being particularly threatening. This study focuses on Sebkha el Kalbia in Tunisia, highlighting the challenges faced by local communities in addressing heavy metal pollution. The area is prone to contamination through rivers and streams that transport pollutants from industrial zones and rural areas into the salt pan. The recent establishment of an industrial zone has worsened pollution levels, calling for strict regulatory measures and clean technologies to limit heavy metal pollution and protect human health and the environment. The study assesses the impact of lead and zinc pollution on the growth of Sulla carnosa and its potential for phytoremediation. Soil and plant samples from contaminated areas were analyzed, revealing high levels of heavy metal contamination. The growth parameters of Sulla carnosa, such as plant height, weight, and enzymatic activity, were examined, showing a significant reduction in plant growth when exposed to high metal concentrations. Specifically, in the presence of 100 ppm of lead (Pb), net photosynthetic assimilation (An) decreased by 52%, while the amount of Pb increased by 78%. At 800 ppm of Pb, An decreased by 87%, and the amount of Pb increased by over 800%. Furthermore, the relationship between net photosynthetic assimilation and lead (Pb) content remained significant but negative. At high doses (800 ppm), the biomass produced decreases by 64%, while the amount of Zn increases 2.7 times. These results suggest that at low doses, zinc is not toxic. These findings highlight Sulla carnosa as a potential candidate for phytoremediation with preferential metal accumulation in the roots and improved enzymatic activity, underscoring the urgency of addressing heavy metal pollution in Sebkha el Kalbia.

Functional Dissection of the Physiological Traits Promoting Durum Wheat (Triticum durum Desf.) Tolerance to Drought Stress.

In Tunisia's arid and semi-arid lands, drought stress remains the most critical factor limiting agricultural production due to low and irregular precipitation. The situation is even more difficult because of the scarcity of underground water for irrigation and the climate change that has intensified and expanded the aridity. One of the most efficient and sustainable approaches to mitigating drought stress is exploring genotypic variability to screen tolerant genotypes and identify useful tolerance traits. To this end, six Tunisian wheat genotypes (Triticum durum Desf.) were cultivated in the field, under a greenhouse and natural light, to be studied for their differential tolerance to drought stress. Many morpho-physiological and biochemical traits were analyzed, and interrelationships were established. Depending on the genotypes, drought stress significantly decreased plant growth, chlorophyll biosynthesis, and photosynthesis; stimulated osmolyte accumulation and disturbed water relations. The most tolerant genotypes (salim and karim) accumulated more potassium (K) and proline in their shoots, allowing them to maintain better tissue hydration and physiological functioning. The osmotic adjustment (OA), in which potassium and proline play a key role, determines wheat tolerance to drought stress. The calculated drought index (DI), drought susceptible index (DSI), drought tolerance index (DTI), K use efficiency (KUE), and water use efficiency (WUE) discriminated the studied genotypes and confirmed the relative tolerance of salim and karim.

Potential of Seed Halopriming in the Mitigation of Salinity Stress during Germination and Seedling Establishment in Durum Wheat (Triticum durum Desf.).

The salinity of soils and irrigation water is among the main factors that limit plant productivity worldwide. Several alternatives have been proposed to get around this problem. However, these alternatives have faced difficulties in their implementation. As an alternative, the adverse effects of salinity on crop yield can be minimized by selecting species and varieties better adapted to salinity and/or by finding priming agents that give plants a certain tolerance during the vegetative and reproductive stages. The latter are strictly dependent on germination and seedling establishment. For this purpose, a laboratory experiment was conducted on three Tunisian wheat cultivars (Karim, Razeg, and Maali) subjected to moderate salinity stress (MSS, 5 g L-1 NaCl), severe salinity stress (SSS, 10 g L-1 NaCl), or control (0 NaCl) after soaking the seeds in a solution of KNO3 or ZnSO4 (0.5 g L-1). Salinity stress significantly decreased germination capacity (GC) and induced osmotic stress under MSS, which declined under SSS in favor of toxic stress. Pretreatment of seeds with KNO3 or ZnSO4 alleviated the toxic effect, and seedlings recovered initial vigor and GC even under SSS. The Karim cultivar showed better tolerance to salinity and a higher ability to react to priming agents. The calculated sensitivity tolerance index (STI) based on germination capacity, seedling growth, and initial vigor decreased in all cultivars under salt stress; however, this parameter clearly discriminated the studied cultivars. Karim was the most tolerant as compared to Razeg and Maali. We conclude that halopriming provides a benefit by alleviating the harmful effects of salt toxicity and that cultivars differ in their response to priming and extent of salt stress. KNO3 and ZnSO4 effectively alleviated the inhibitory effect of salt stress on seed germination and seedling establishment while significantly improving initial vigor.

Genotypic differences in response of durum wheat (Triticum durum Desf.) to lime-induced iron chlorosis.

Wheat, durum wheat, is the first cereal cultivated and consumed in Tunisia. Because the dominance of calcareous soils in its agroecological systems, known by their low availability of iron (Fe) inducing Fe chlorosis and limiting crop production, its yield remains low. Therefore, the search for tolerant genotypes is always current. In this context, the physiological behavior of six Tunisian genotypes of durum wheat (salim, karim, razek, khiar, inrat100, and maali) cultivated on calcareous and fertile soils for 2 months in a pot experiment was investigated. A greenhouse was used to conduct experiments under natural light. Plant growth, SPAD index, Fe nutrition, Fe distribution, and photosynthesis were monitored and used to evaluate and discriminate their respective physiological responses. On calcareous soil, results revealed reduced plant growth, active Fe, SPAD index, and net photosynthesis. Genotypic differences in the response of wheat to calcareous-induced Fe deficiency were observed and allowed to classify the genotypes Salim and Karim as relatively tolerant. These genotypes expressed Fe translocation capacity (FeT) up to 3 times, Fe use efficiency for photosynthesis (FeUEAn) up to 1.6 times, and chlorophyll use efficiency for photosynthesis (ChlUEAn) up to 3.5 times greater than that expressed by the other genotypes, particularly inrat100 and maali. Thus, the relative tolerance of Salim and Karim is the result of the high ability of Fe uptake and translocation to shoots to support chlorophyll biosynthesis, photosynthesis, and plant growth as well as an important Fe and chlorophyll use efficiency.

Chemical seed priming alleviates salinity stress and improves Sulla carnosa germination in the saline depression of Tunisia.

In the saline depressions (Sebkhas) of Tunisia, Sulla carnosa expresses anarchic distribution, sometimes in association with halophytes sometimes individually. In order to explain this distribution, we investigated the effects of salinity on seed germination, the osmotic and toxic limiting factors, and the importance of some stimulating agents (priming agents) in the improvement of the tolerance to salinity at the germinating stage. A study was conducted on seeds harvested from the natural biotope Sebkha d'El Kelbia (35°50'34″N, 10°16'18″E), and an increasing concentration of NaCl (0, 5, 10, 15, and 20 g L-1) was applied. Some priming agents were used to propose efficient, rapid, and low-cost tools to improve the seed germination and tolerance of Sulla carnosa (Desf.) in saline depression. Salinity stress significantly decreased germination capacity and rate and delayed its initiation and maximum. Until 15-g L-1 NaCl, the most limiting factor of seed germination is the osmotic effect. At 20-g L-1 NaCl, the toxic effect dominates, and germination is irreversibly inhibited. Some priming agents have shown their efficiency in improving the germination capacity at 10-g L-1 NaCl and conferring a salt tolerance of up to 15-g L-1 NaCl.

Potential of the physiological response of pea plants (Pisum sativum L. ) to iron deficiency (direct or lime- induced) / Potencial da resposta fisiológica das plantas de ervilha (Pisum sativum L. ) à deficiência de ferro (direta ou induzida pelo calcário)

Pea (Pisum sativum L.) is an important food crop in Tunisia, where calcareous soils represents the major limiting factor for agriculture production. In the present study a greenhouse experiment was conducted to assess the effects of direct and bicarbonate- induced iron deficiency on plant growth, chlorophyll fluorescence, photosynthesis, spad index and iron nutrition in two Tunisian pea genotypes (Pisum sativum L.). Plants were grown hydroponically and iron deficiency was induced for 3 weeks. Iron deficiency decreased all the above physiological parameters. The direct Fe deficiency is more drastic than bicarbonate- induced Fe deficiency. A close relationship between plant growth, photosynthesis and SPAD index was observed. Fe use efficiency for plant growth and Fe use efficiency for photosynthesis discriminates clearly the studied genotypes and seems to be the main reason of the tolerance of Kelvedon, as compared to Lincoln.

A ervilha (Pisum sativum L.) é uma cultura alimentar importante na Tunísia, onde os solos calcários representam o principal fator limitante para a produção agrícola. No presente estudo, foi conduzido um experimento em estufa para avaliar os efeitos da deficiência de ferro direta e induzida por bicarbonato sobre o crescimento de plantas, a fluorescência da clorofila, a fotossíntese, o índice SPAD e a nutrição de ferro em dois genótipos de ervilha da Tunísia (Pisum sativum L.). As plantas foram cultivadas hidroponicamente e a deficiência de ferro foi induzida durante 3 semanas. A deficiência de ferro diminuiu todos os parâmetros fisiológicos acima. A deficiência de Fe direta é mais drástica do que a deficiência de Fe induzida por bicarbonato. Observou-se uma estreita relação entre o crescimento das plantas, a fotossíntese e o índice SPAD. A eficiência de uso de Fe para o crescimento de plantas e a eficiência de uso de Fe para a fotossíntese discriminam claramente os genótipos estudados e parecem ser a razão principal da tolerância de Kelvedon, em comparação com a de Lincoln.

Genotypic variation of N2-fixing common bean (Phaseolus vulgaris L.) in response to iron deficiency.

In calcareous soils, the yield of grain legumes is often limited by the lower availability of iron (Fe), especially when they depend upon symbiosis with root nodule bacteria for their N nutrition. In order to explore the variability of responses of N(2)-fixing common bean to Fe deficiency the common bean white-seeded lines Striker and Coco blanc, and coloured-seeded lines SVM-29-21 and ARA14 were inoculated with Rhizobium tropici (CIAT 899) and cultivated hydroaeroponically with a N-free nutrient solution supplied or not with 45microM Fe. Differences among lines were observed: Fe-deficiency-induced-chlorosis on young leaves was earlier and more severe in some lines than others. Nodule development and N(2)-fixing capacity was less affected in line ARA14 which preferentially allocated Fe towards nodules. Results suggest that Fe use efficiency for symbiotic nitrogen fixation (FeUE SNF) could be used to screen tolerant bean lines to Fe deficiency in condition of symbiotic nitrogen fixation.