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1.
J Biol Chem ; 283(16): 10679-89, 2008 Apr 18.
Artículo en Inglés | MEDLINE | ID: mdl-18276589

RESUMEN

Collagen prolyl 4-hydroxylases (C-P4Hs) have a critical role in collagen synthesis, since 4-hydroxyproline residues are necessary for folding of the triple-helical molecules. Vertebrate C-P4Hs are alpha(2)beta(2) tetramers in which the beta subunit is identical to protein-disulfide isomerase (PDI). Three isoforms of the catalytic alpha subunit, PHY-1, PHY-2, and PHY-3, have been characterized from Caenorhabditis elegans, PHY-1 and PHY-2 being responsible for the hydroxylation of cuticle collagens, whereas PHY-3 is predicted to be involved in collagen synthesis in early embryos. We have characterized transcripts of two additional C. elegans alpha subunit-like genes, Y43F8B.4 and C14E2.4. Three transcripts were generated from Y43F8B.4, and a polypeptide encoded by one of them, named PHY-4.1, assembled into active (PHY-4.1)(2)/(PDI-2)(2) tetramers and PHY-4.1/PDI-2 dimers when coexpressed with C. elegans PDI-2 in insect cells. The C14E2.4 transcript was found to have a frameshift leading to the absence of codons for two residues critical for P4H catalytic activity. Thus, C. elegans has altogether four functional C-P4H alpha subunits, PHY-1, PHY-2, PHY-3, and PHY-4.1. The tetramers and dimers containing recombinant PHY-4.1 had a distinct substrate specificity from the other C-P4Hs in that they hydroxylated poly(l-proline) and certain other proline-rich peptides, including ones that are expressed in the pharynx, in addition to collagen-like peptides. These data and the observed restricted expression of the phy-4.1 transcript and PHY-4.1 polypeptide in the pharyngeal gland cells and the excretory duct suggest that in addition to collagens, PHY-4.1 may hydroxylate additional proline-rich proteins in vivo.


Asunto(s)
Caenorhabditis elegans/metabolismo , Regulación Enzimológica de la Expresión Génica , Faringe/enzimología , Procolágeno-Prolina Dioxigenasa/química , Procolágeno-Prolina Dioxigenasa/fisiología , Secuencia de Aminoácidos , Animales , Catálisis , Colágeno/química , Modelos Biológicos , Datos de Secuencia Molecular , Procolágeno-Prolina Dioxigenasa/biosíntesis , Procolágeno-Prolina Dioxigenasa/metabolismo , Prolina/química , Estructura Terciaria de Proteína , Interferencia de ARN , Proteínas Recombinantes/química , Especificidad por Sustrato
2.
Matrix Biol ; 26(5): 382-95, 2007 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-17321733

RESUMEN

The collagen prolyl 4-hydroxylases (P4Hs) are essential for proper extracellular matrix formation in multicellular organisms. The vertebrate enzymes are alpha(2)beta(2) tetramers, in which the beta subunits are identical to protein disulfide isomerase (PDI). Unique P4H forms have been shown to assemble from the Caenorhabditis elegans catalytic alpha subunit isoforms PHY-1 and PHY-2 and the beta subunit PDI-2. A mixed PHY-1/PHY-2/(PDI-2)(2) tetramer is the major form, while PHY-1/PDI-2 and PHY-2/PDI-2 dimers are also assembled but less efficiently. Cloning and characterization of the orthologous subunits from the closely related nematode Caenorhabditis briggsae revealed distinct differences in the assembly of active P4H forms in spite of the extremely high amino acid sequence identity (92-97%) between the C. briggsae and C. elegans subunits. In addition to a PHY-1/PHY-2(PDI-2)(2) tetramer and a PHY-1/PDI-2 dimer, an active (PHY-2)(2)(PDI-2)(2) tetramer was formed in C. briggsae instead of a PHY-2/PDI-2 dimer. Site-directed mutagenesis studies and generation of inter-species hybrid polypeptides showed that the N-terminal halves of the Caenorhabditis PHY-2 polypeptides determine their assembly properties. Genetic disruption of C. briggsae phy-1 (Cb-dpy-18) via a Mos1 insertion resulted in a small (short) phenotype that is less severe than the dumpy (short and fat) phenotype of the corresponding C. elegans mutants (Ce-dpy-18). C. briggsae phy-2 RNA interference produced no visible phenotype in the wild type nematodes but produced a severe dumpy phenotype and larval arrest in phy-1 mutants. Genetic complementation of the C. briggsae and C. elegans phy-1 mutants was achieved by injection of a wild type phy-1 gene from either species.


Asunto(s)
Caenorhabditis/metabolismo , Colágeno/metabolismo , Procolágeno-Prolina Dioxigenasa/química , Procolágeno-Prolina Dioxigenasa/metabolismo , Subunidades de Proteína/química , Secuencias de Aminoácidos , Secuencia de Aminoácidos , Animales , Caenorhabditis/genética , Catálisis , Prueba de Complementación Genética , Datos de Secuencia Molecular , Péptidos/química , Mutación Puntual , Procolágeno-Prolina Dioxigenasa/genética , Isoformas de Proteínas , Interferencia de ARN , Homología de Secuencia de Aminoácido , Especificidad de la Especie
3.
J Biol Chem ; 279(18): 18656-61, 2004 Apr 30.
Artículo en Inglés | MEDLINE | ID: mdl-14985345

RESUMEN

The collagen prolyl 4-hydroxylases (collagen P4Hs, EC 1.14.11.2) play a key role in the synthesis of the extracellular matrix. The vertebrate enzymes are alpha(2)beta(2) tetramers, the beta subunit being identical to protein disulfide isomerase (PDI). The main Caenorhabditis elegans collagen P4H form is an unusual PHY-1/PHY-2/(PDI)(2) mixed tetramer consisting of two types of catalytic alpha subunit, but the PHY-1 and PHY-2 polypeptides also form active PHY/PDI dimers. The lengths of peptide substrates have a major effect on their interaction with the P4H tetramers, the K(m) values decreasing markedly with increasing chain length. This phenomenon has been explained in terms of processive binding of the two catalytic subunits to long peptides. We determined here the K(m) values of a collagen P4H having two catalytic sites, the C. elegans mixed tetramer, and a form having only one such site, the PHY-1/PDI dimer, for peptides of varying lengths. All the K(m) values of the PHY-1/PDI dimer were found to be about 1.5-2.5 times those of the tetramer, but increasing peptide length led to identical decreases in the values of both enzyme forms. The K(m) for a nonhydroxylated collagen fragment with 33 -X-Y-Gly-triplets but only 11 -X-Pro-Gly-triplets was found to correspond to the number of the former rather than the latter. To study the individual roles of the two catalytic sites in a tetramer, we produced mutant PHY-1/PHY-2/(PDI)(2) tetramers in which binding of the Fe(2+) ion or 2-oxoglutarate to one of the two catalytic sites was prevented. The activities of the mutant tetramers decreased to markedly less than 50% of that of the wild type, being about 5-10% and 20-30% with the enzymes having one of the two Fe(2+)-binding sites or 2-oxoglutarate-binding sites inactivated, respectively, while the K(m) values for these cosubstrates or peptide substrates were not affected. Our data thus indicate that although collagen P4Hs do not act on peptide substrates by a processive mechanism, prevention of hydroxylation at one of the two catalytic sites in the tetramer impairs the function of the other catalytic site.


Asunto(s)
Proteínas de Caenorhabditis elegans/metabolismo , Péptidos/metabolismo , Procolágeno-Prolina Dioxigenasa/química , Procolágeno-Prolina Dioxigenasa/metabolismo , Sustitución de Aminoácidos , Animales , Proteínas de Caenorhabditis elegans/química , Proteínas de Caenorhabditis elegans/genética , Dominio Catalítico , Dimerización , Humanos , Hidroxilación , Cinética , Procolágeno-Prolina Dioxigenasa/genética , Isoformas de Proteínas , Estructura Cuaternaria de Proteína , Subunidades de Proteína , Especificidad por Sustrato
4.
J Biol Chem ; 278(48): 47685-93, 2003 Nov 28.
Artículo en Inglés | MEDLINE | ID: mdl-14500733

RESUMEN

Collagen prolyl 4-hydroxylases (C-P4Hs) catalyze the formation of 4-hydroxyproline by the hydroxylation of -X-Pro-Gly-triplets. The vertebrate enzymes are alpha 2 beta 2 tetramers, the beta-subunit being identical to protein-disulfide isomerase (PDI). Two isoforms of the catalytic alpha-subunit, which combine with PDI to form [alpha(I)]2 beta 2 and [alpha(II)]2 beta 2 tetramers, have been known up to now. We report here on the cloning and characterization of a third vertebrate C-P4H alpha-subunit isoform, alpha(III). The processed human, rat and mouse alpha(III) polypeptides consist of 520-525 residues, all three having signal peptides of 19-22 additional residues. The sequence of the processed human alpha(III) polypeptide is 35-37% identical to those of human alpha(I) and alpha(II), the highest identity being found within the catalytically important C-terminal region and all five critical residues at the cosubstrate binding sites being conserved. The sequence within a region corresponding to the peptide-substrate binding domain is less conserved, but all five alpha helices constituting this domain can be predicted to be located in identical positions in alpha(I), alpha(II), and alpha(III) and to have essentially identical lengths. The alpha(III) mRNA is expressed in many human tissues, but at much lower levels than the alpha(I) and alpha(II) mRNAs. In contrast to alpha(I) and alpha(II), no evidence was found for alternative splicing of the alpha(III) transcripts. Coexpression of a recombinant human alpha(III) polypeptide with PDI in human embryonic kidney cells led to the formation of an active enzyme that hydroxylated collagen chains and a collagen-like peptide and appeared to be an [alpha(III)]2 beta 2 tetramer. The catalytic properties of the recombinant enzyme were very similar to those of the type I and II C-P4Hs, with the exception that its peptide binding properties were intermediate between those of the type I and type II enzymes.


Asunto(s)
Procolágeno-Prolina Dioxigenasa/química , Secuencia de Aminoácidos , Animales , Sitios de Unión , Northern Blotting , Catálisis , Dominio Catalítico , Línea Celular , Clonación Molecular , Colágeno/química , Cisteína/química , ADN Complementario/metabolismo , Relación Dosis-Respuesta a Droga , Electroforesis en Gel de Poliacrilamida , Humanos , Immunoblotting , Concentración 50 Inhibidora , Insectos , Cinética , Ratones , Modelos Genéticos , Datos de Secuencia Molecular , Péptido-N4-(N-acetil-beta-glucosaminil) Asparagina Amidasa/química , Péptidos/química , Pruebas de Precipitina , Isoformas de Proteínas , Señales de Clasificación de Proteína , Estructura Terciaria de Proteína , ARN Mensajero/metabolismo , Ratas , Proteínas Recombinantes/química , Homología de Secuencia de Aminoácido , Distribución Tisular , beta-Galactosidasa/metabolismo
5.
J Biol Chem ; 278(37): 34966-74, 2003 Sep 12.
Artículo en Inglés | MEDLINE | ID: mdl-12824157

RESUMEN

The collagen prolyl 4-hydroxylases (C-P4Hs) catalyze the formation of 4-hydroxyproline by the hydroxylation of proline residues in -Xaa-Pro-Gly-sequences. The vertebrate enzymes are alpha 2 beta 2 tetramers in which protein-disulfide isomerase serves as the beta subunit. Two isoforms of the catalytic alpha subunit have been identified and shown to form [alpha(I)]2 beta 2 and [alpha(II)]2 beta 2 tetramers, the type I and type II C-P4Hs, respectively. The peptide-substrate-binding domain of type I C-P4H has been shown to be located between residues 138 and 244 in the 517-residue alpha(I) subunit and to be distinct from the catalytic domain that is located in the C-terminal region. We report here that a recombinant human C-P4H alpha(I) polypeptide Phe144-Ser244 forms a folded domain consisting of five alpha helices and one short beta strand. This structure is quite different from those of other proline-rich peptide-binding modules, which consist mainly of beta strands. Binding of the peptide (Pro-Pro-Gly)2 to this domain caused major chemical shifts in many backbone amide resonances, the residues showing the largest shifts being mainly hydrophobic, including three tyrosines. The Kd values determined by surface plasmon resonance and isothermal titration calorimetry for the binding of several synthetic peptides to the alpha(I) and the corresponding alpha(II) domain were very similar to the Km and Ki values for these peptides as substrates and inhibitors of the type I and type II C-P4H tetramers. The Kd values of the alpha(I) and alpha(II) domains for (Gly-Pro-4Hyp)5 were much higher than those for (Pro-Pro-Gly)5, indicating a marked decrease in the affinity of hydroxylated peptides for the domain. Many characteristic features of the binding of peptides to the type I and type II C-P4H tetramers can thus be explained by the properties of binding to this domain rather than the catalytic domain.


Asunto(s)
Colágeno/metabolismo , Péptidos/metabolismo , Procolágeno-Prolina Dioxigenasa/química , Procolágeno-Prolina Dioxigenasa/metabolismo , Prolina , Secuencia de Aminoácidos , Sitios de Unión , Dicroismo Circular , Humanos , Datos de Secuencia Molecular , Péptidos/química , Conformación Proteica , Estructura Secundaria de Proteína , Subunidades de Proteína/química , Subunidades de Proteína/metabolismo , Proteínas Recombinantes/química , Proteínas Recombinantes/metabolismo , Alineación de Secuencia , Homología de Secuencia de Aminoácido , Especificidad por Sustrato
6.
J Biol Chem ; 277(32): 29187-96, 2002 Aug 09.
Artículo en Inglés | MEDLINE | ID: mdl-12036960

RESUMEN

The collagen prolyl 4-hydroxylases (P4Hs, EC ) play a critical role in the synthesis of the extracellular matrix. The enzymes characterized from vertebrates and Drosophila are alpha(2)beta(2) tetramers, in which protein disulfide isomerase (PDI) serves as the beta subunit. Two conserved alpha subunit isoforms, PHY-1 and PHY-2, have been identified in Caenorhabditis elegans. We report here that three unique P4H forms are assembled from these polypeptides and the single beta subunit PDI-2, both in a recombinant expression system and in vivo, namely a PHY-1/PHY-2/(PDI-2)(2) mixed tetramer and PHY-1/PDI-2 and PHY-2/PDI-2 dimers. The mixed tetramer is the main P4H form in wild-type C. elegans but phy-2-/- and phy-1-/- (dpy-18) mutant nematodes can compensate for its absence by increasing the assembly of the PHY-1/PDI-2 and PHY-2/PDI-2 dimers, respectively. All three of the mixed tetramer-forming polypeptides PHY-1, PHY-2, and PDI-2 are coexpressed in the cuticle collagen-synthesizing hypodermal cells. The catalytic properties of the mixed tetramer are similar to those of other P4Hs, and analogues of 2-oxoglutarate were found to produce severe temperature-dependent effects on P4H mutant strains. Formation of the novel mixed tetramer was species-specific, and studies with hybrid recombinant PHY polypeptides showed that residues Gln(121)-Ala(271) and Asp(1)-Leu(122) in PHY-1 and PHY-2, respectively, are critical for its assembly.


Asunto(s)
Proteínas de Caenorhabditis elegans/química , Caenorhabditis elegans/metabolismo , Colágeno/metabolismo , Procolágeno-Prolina Dioxigenasa/metabolismo , Animales , Baculoviridae/metabolismo , Catálisis , Línea Celular , Detergentes/farmacología , Dimerización , Electroforesis en Gel de Poliacrilamida , Insectos , Cinética , Microscopía Fluorescente , Octoxinol/farmacología , Péptidos/química , Unión Proteica , ARN/metabolismo , Proteínas Recombinantes/metabolismo
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