Injectable Liposome-Loaded Hydrogel Formulations with Controlled Release of Curcumin and α-Tocopherol for Dental Tissue Engineering.

An injectable hydrogel formulation is developed utilizing low- and high-molecular-weight chitosan (LCH and HCH) incorporated with curcumin and α-tocopherol-loaded liposomes (Lip/Cur+Toc). Cur and Toc releases are delayed within the hydrogels. The injectability of hydrogels is proved via rheological analyses. In vitro studies are conducted using human dental pulp stem cells (hDPSCs) and human gingival fibroblasts (hGFs) to examine the biological performance of the hydrogels toward endodontics and periodontics, respectively. The viability of hDPSCs treated with the hydrogels with Lip/Cur+Toc is the highest till day 14, compared to the neat hydrogels. During odontogenic differentiation tests, alkaline phosphatase (ALP) enzyme activity of hDPSCs is induced in the Cur-containing groups. Biomineralization is enhanced mostly with Lip/Cur+Toc incorporation. The viability of hGFs is the highest in HCH combined with Lip/Cur+Toc while wound healing occurs almost 100% in both (Lip/Cur+Toc@LCH and Lip/Cur+Toc@HCH) after 2 days. Antioxidant activity of Lip/Cur+Toc@LCH on hGFs is significantly the highest among the groups. Antimicrobial tests demonstrate that Lip/Cur+Toc@LCH is more effective against Escherichia coli whereas so is Lip/Cur+Toc@HCH against Staphylococcus aureus. The antimicrobial mechanism of the hydrogels is investigated for the first time through various computational models. LCH and HCH loaded with Lip/Cur+Toc are promising candidates with multi-functional features for endodontics and periodontics.

Cyanophycin modifications for applications in tissue scaffolding.

Cyanophycin (CGP) is a polypeptide consisting of amino acids-aspartic acid in the backbone and arginine in the side chain. Owing to its resemblance to cell adhesive motifs in the body, it can be considered suitable for use in biomedical applications as a novel component to facilitate cell attachment and tissue regeneration. Although it has vast potential applications, starting with nutrition, through drug delivery and tissue engineering to the production of value-added chemicals and biomaterials, CGP has not been brought to the industry yet. To develop scaffolds using CGP powder produced by bacteria, its properties (e.g., biocompatibility, morphology, biodegradability, and mechanical strength) should be tailored in terms of the requirements of the targeted tissue. Crosslinking commonly stands for a primary modification method for renovating biomaterial features to these extents. Herein, we aimed to crosslink CGP for the first time and present a comparative study of different methods of CGP crosslinking including chemical, physical, and enzymatic methods by utilizing glutaraldehyde (GTA), UV exposure, genipin, 1-ethyl-3-[3-dimethylaminopropyl] carbodiimide hydrochloride/N-hydroxysuccinimide (EDC/NHS), and monoamine oxidase (MAO). Crosslinking efficacy varied among the samples crosslinked via the different crosslinking methods. All crosslinked CGP were non-cytotoxic to L929 cells, except for the groups with higher GTA concentrations. We conclude that CGP is a promising candidate for scaffolding purposes to be used as part of a composite with other biomaterials to maintain the integrity of scaffolds. The initiative study demonstrated the unknown characteristics of crosslinked CGP, even though its feasibility for biomedical applications should be confirmed by further examinations. KEY POINTS: • Cyanophycin was crosslinked by 5 different methods • Crosslinked cyanophycin is non-cytotoxic to L929 cells • Crosslinked cyanophycin is a promising new material for scaffolding purposes.

Advances in antimicrobial hydrogels for dental tissue engineering: regenerative strategies for endodontics and periodontics.

Dental tissue infections have been affecting millions of patients globally leading to pain, severe tissue damage, or even tooth loss. Commercial sterilizers may not be adequate to prevent frequent dental infections. Antimicrobial hydrogels have been introduced as an effective therapeutic strategy for endodontics and periodontics since they have the capability of imitating the native extracellular matrix of soft tissues. Hydrogel networks are considered excellent drug delivery platforms due to their high-water retention capacity. In this regard, drugs or nanoparticles can be incorporated into the hydrogels to endow antimicrobial properties as well as to improve their regenerative potential, once biocompatibility criteria are met avoiding high dosages. Herein, novel antimicrobial hydrogel formulations were discussed for the first time in the scope of endodontics and periodontics. Such hydrogels seem outstanding candidates especially when designed not only as simple volume fillers but also as smart biomaterials with condition-specific adaptability within the dynamic microenvironment of the defect site. Multifunctional hydrogels play a pivotal role against infections, inflammation, oxidative stress, etc. along the way of dental regeneration. Modern techniques (e.g., 3D and 4D-printing) hold promise to develop the next generation of antimicrobial hydrogels together with their limitations such as infeasibility of implantation.

Antimicrobial Activities of Polyethylene Terephthalate-Waste-Derived Nanofibrous Membranes Decorated with Green Synthesized Ag Nanoparticles.

Thermoplastic polymers are one of the synthetic materials produced with high tonnage in the world and are so omnipresent in industries and everyday life. One of the most important polymeric wastes is polyethylene terephthalate (PET), and the disposal of used PET bottles is an unsolved environmental problem, and many efforts have been made to find practical solutions to solve it. In this present work, nanofibrous membranes were produced from waste PET bottles using the electrospinning process. The surface of membranes was modified using NaOH and then decorated with green synthesized Ag nanoparticles (10 ± 2 nm) using an in situ chemical reduction method. The morphology, size, and diameter of the Ag nanoparticles decorating the nanofibers were characterized through transmission electron microscopy (TEM), a field emission scanning electron microscope (FESEM), Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), and UV-visible spectroscopy techniques. Finally, the antimicrobial activity of the nanofibrous membranes was tested against Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus using disc diffusion and colony-forming count methods. The growth of bacteria was not affected by the pure nanofibrous membranes, while the Ag-decorated samples showed inhibition zones of 17 ± 1, 16 ± 1, and 14 ± 1 mm for Pseudomonas aeruginosa, Escherichia coli, and Staphylococcus aureus, respectively. The planktonic culture results of Pseudomonas aeruginosa showed that the membranes had a relatively low inhibitory effect on its growth. The obtained results showed that Pseudomonas aeruginosa has a relatively low ability to form biofilms on the nanostructured membranes too. A good agreement was observed between the data of biofilm formation and the planktonic cultures of bacteria. The plastic-waste-derived PET/Ag nanocomposite membranes can be used for wound dressings, air filters, and water purification applications.

Antimicrobial and Biodegradable 3D Printed Scaffolds for Orthopedic Infections.

In bone tissue engineering, the performance of scaffolds underpins the success of the healing of bone. Microbial infection is the most challenging issue for orthopedists. The application of scaffolds for healing bone defects is prone to microbial infection. To address this challenge, scaffolds with a desirable shape and significant mechanical, physical, and biological characteristics are crucial. 3D printing of antibacterial scaffolds with suitable mechanical strength and excellent biocompatibility is an appealing strategy to surmount issues of microbial infection. The spectacular progress in developing antimicrobial scaffolds, along with beneficial mechanical and biological properties, has sparked further research for possible clinical applications. Herein, the significance of antibacterial scaffolds designed by 3D, 4D, and 5D printing technologies for bone tissue engineering is critically investigated. Materials such as antibiotics, polymers, peptides, graphene, metals/ceramics/glass, and antibacterial coatings are used to impart the antimicrobial features for the 3D scaffolds. Polymeric or metallic biodegradable and antibacterial 3D-printed scaffolds in orthopedics disclose exceptional mechanical and degradation behavior, biocompatibility, osteogenesis, and long-term antibacterial efficiency. The commercialization aspect of antibacterial 3D-printed scaffolds and technical challenges are also discussed briefly. Finally, the discussion on the unmet demands and prevailing challenges for ideal scaffold materials for fighting against bone infections is included along with a highlight of emerging strategies in this field.

Microbial electrosynthesis: carbonaceous electrode materials for CO2 conversion.

Microbial electrosynthesis (MES) is a sustainable approach to address greenhouse gas (GHG) emissions using anthropogenic carbon dioxide (CO2) as a building block to create clean fuels and highly valuable chemicals. The efficiency of MES-based CO2 conversion is closely related to the performance of electrode material and, in particular, the cathode for which carbonaceous materials are frequently used. Compared to expensive metal electrodes, carbonaceous materials are biocompatible with a high specific surface area, wide range of possible morphologies, and excellent chemical stability, and their use can maximize the growth of bacteria and enhance electron transfer rates. Examples include MES cathodes based on carbon nanotubes, graphene, graphene oxide, graphite, graphite felt, graphitic carbon nitride (g-C3N4), activated carbon, carbon felt, carbon dots, carbon fibers, carbon brushes, carbon cloth, reticulated vitreous carbon foam, MXenes, and biochar. Herein, we review the state-of-the-art MES, including thermodynamic and kinetic processes that underpin MES-based CO2 conversion, as well as the impact of reactor type and configuration, selection of biocompatible electrolytes, product selectivity, and the use of novel methods for stimulating biomass accumulation. Specific emphasis is placed on carbonaceous electrode materials, their 3D bioprinting and surface features, and the use of waste-derived carbon or biochar as an outstanding material for further improving the environmental conditions of CO2 conversion using carbon-hungry microbes and as a step toward the circular economy. MES would be an outstanding technique to develop rocket fuels and bioderived products using CO2 in the atmosphere for the Mars mission.

Antimicrobial TiO2 nanocomposite coatings for surfaces, dental and orthopaedic implants.

Engineering of self-disinfecting surfaces to constrain the spread of SARS-CoV-2 is a challenging task for the scientific community because the human coronavirus spreads through respiratory droplets. Titania (TiO2) nanocomposite antimicrobial coatings is one of the ideal remedies to disinfect pathogens (virus, bacteria, fungi) from common surfaces under light illumination. The photocatalytic disinfection efficiency of recent TiO2 nanocomposite antimicrobial coatings for surfaces, dental and orthopaedic implants are emphasized in this review. Mostly, inorganic metals (e.g. copper (Cu), silver (Ag), manganese (Mn), etc), non-metals (e.g. fluorine (F), calcium (Ca), phosphorus (P)) and two-dimensional materials (e.g. MXenes, MOF, graphdiyne) were incorporated with TiO2 to regulate the charge transfer mechanism, surface porosity, crystallinity, and the microbial disinfection efficiency. The antimicrobial activity of TiO2 coatings was evaluated against the most crucial pathogenic microbes such as Escherichia coli, methicillin-resistant Staphylococcus aureus, Pseudomonas aeruginosa, Bacillus subtilis, Legionella pneumophila, Staphylococcus aureus, Streptococcus mutans, T2 bacteriophage, H1N1, HCoV-NL63, vesicular stomatitis virus, bovine coronavirus. Silane functionalizing agents and polymers were used to coat the titanium (Ti) metal implants to introduce superhydrophobic features to avoid microbial adhesion. TiO2 nanocomposite coatings in dental and orthopaedic metal implants disclosed exceptional bio-corrosion resistance, durability, biocompatibility, bone-formation capability, and long-term antimicrobial efficiency. Moreover, the commercial trend, techno-economics, challenges, and prospects of antimicrobial nanocomposite coatings are also discussed briefly.

Carbonaceous cathode materials for electro-Fenton technology: Mechanism, kinetics, recent advances, opportunities and challenges.

Electro-Fenton (EF) technique has gained significant attention in recent years owing to its high efficiency and environmental compatibility for the degradation of organic pollutants and contaminants of emerging concern (CECs). The efficiency of an EF reaction relies primarily on the formation of hydrogen peroxide (H2O2) via 2e─ oxygen reduction reaction (ORR) and the generation of hydroxyl radicals (●OH). This could be achieved through an efficient cathode material which operates over a wide pH range (pH 3-9). Herein, the current progresses on the advancements of carbonaceous cathode materials for EF reactions are comprehensively reviewed. The insights of various materials such as, activated carbon fibres (ACFs), carbon/graphite felt (CF/GF), carbon nanotubes (CNTs), graphene, carbon aerogels (CAs), ordered mesoporous carbon (OMCs), etc. are discussed inclusively. Transition metals and hetero atoms were used as dopants to enhance the efficiency of homogeneous and heterogeneous EF reactions. Iron-functionalized cathodes widened the working pH window (pH 1-9) and limited the energy consumption. The mechanism, reactor configuration, and kinetic models, are explained. Techno economic analysis of the EF reaction revealed that the anode and the raw materials contributed significantly to the overall cost. It is concluded that most reactions follow pseudo-first order kinetics and rotating cathodes provide the best H2O2 production efficiency in lab scale. The challenges, future prospects and commercialization of EF reaction for wastewater treatment are also discussed.