In-situ biofabrication of bacterial nanocellulose (BNC)/graphene oxide (GO) nano-biocomposite and study of its cationic dyes adsorption properties.

In the present study, bacterial nanocellulose/graphene oxide nano-biocomposites (BNC-GO-NBCs) were fabricated by Komagataeibacter saccharivorans NUWB1 using an in-situ method involving three time-dependent approaches. Physicochemical studies showed that the chosen dried BNC-GO-NBC possessed a three-dimensional interconnected porous structure of BNC with GO layers embedded within the BNC fibrils. BNC-GO-NBC had a crystallinity index of 74.21 %, higher thermostability up to 380 °C and could withstand a tensile load of 84.72 MPa. N2 adsorption-desorption isotherm of the BNC-GO-NBC was found to be of type IV, suggesting a mesoporous type structure with a total pore volume and surface area of 6.232e-04 cc g-1 and 10.498 m2. BNC-GO-NBC exhibited remarkable adsorption capacity for two cationic dyes, Rhodamine B (RhB) and Acridine Orange (AO), and the adsorption data conformed well to the Langmuir isotherm (R2 = 0.99) and pseudo-second-order model. Thermodynamic studies indicated that the adsorption process was spontaneous and endothermic. Additionally, the BNC-GO-NBC displayed the potential for regeneration, with the ability to be recycled up to five times. Further, the antibacterial activity, cell cytotoxicity and oxidative stress assays of the BNC-GO-NBC revealed its non-cytotoxic nature. The findings of the present investigation evidently suggest the potentiality of BNC-GO-NBC in the application of dye adsorption and other environmental applications.

Edible Mushrooms as Novel Myco-Therapeutics: Effects on Lipid Level, Obesity and BMI.

Obesity, usually indicated by a body mass index of more than 30 kg/m2, is a worsening global health issue. It leads to chronic diseases, including type II diabetes, hypertension, and cardiovascular diseases. Conventional treatments for obesity include physical activity and maintaining a negative energy balance. However, physical activity alone cannot determine body weight as several other factors play a role in the overall energy balance. Alternatively, weight loss may be achieved by medication and surgery. However, these options can be expensive or have side effects. Therefore, dietary factors, including dietary modifications, nutraceutical preparations, and functional foods have been investigated recently. For example, edible mushrooms have beneficial effects on human health. Polysaccharides (essentially ß-D-glucans), chitinous substances, heteroglycans, proteoglycans, peptidoglycans, alkaloids, lactones, lectins, alkaloids, flavonoids, steroids, terpenoids, terpenes, phenols, nucleotides, glycoproteins, proteins, amino acids, antimicrobials, and minerals are the major bioactive compounds in these mushrooms. These bioactive compounds have chemo-preventive, anti-obesity, anti-diabetic, cardioprotective, and neuroprotective properties. Consumption of edible mushrooms reduces plasma triglyceride, total cholesterol, low-density lipoprotein, and plasma glucose levels. Polysaccharides from edible mushrooms suppress mRNA expression in 3T3-L1 adipocytes, contributing to their anti-obesity properties. Therefore, edible mushrooms or their active ingredients may help prevent obesity and other chronic ailments.

Anatomical and Physiological Responses of Citrus megaloxycarpa Lush.: a Cryptic Species of Northeast India.

In the historical mysteries and present pandemic situation, the use of citrus fruits makes it rise high among other fruits. Citrus has a significant role in dietary and medicinal purposes from time immemorial and widely acknowledged for its therapeutic properties. Citrus megaloxycarpa Lush. is an unspecified sibling of the citrus family. The present work highlights the biochemical, antimicrobial, and anticancerous potential of cryptic species indigenous to Northeast India. The research was done on peel; P(L1) and pulp; Pu(L2) extracts of ripe large and peel; P(L1) and pulp; Pu(L2) extracts unripe small varieties respectively. The extract of the Pu(L2) has the highest total soluble sugar (9.174±0.006741 µg/ml) whereas the extract of P(S1) demonstrated high protein concentration (8.074±0.0567 µg/ml). The total carbohydrate content also varied in the extracts; the extract of P(L1) showed (8.326±0.003844 µg/ml). P(L1) have high free amino acid content (24.35±0.0225µg/ml) and high free fatty acid exhibited on P(L2) (0.3739±0.05774 µg/ml). The total DPPH scavenging activity was compared for the extracts, where the extract of Pu (S1) exhibits highest activity 73.80% and 0.6577 of logIC50 value. The highest total antioxidant capacity displays 150±0.333 in P(L1). The MIC value was calibrated (30%, 35%, 40%, 45%) (v/v) and found to be maximum in P(L2) (0.695) and minimum in P(L1) (0.163) against Salmonella typhi and Escherichia coli. MTT assay showed highest viability rate of 94.32% and toxicity rate of 8.56% achieved on mouse lung cancerous cell. It is quite obvious from the present research that Citrus megaloxycarpa Lush. has a great scope at industrial level for developing therapeutic drugs.

Development, validation, and feasibility of a school-based short duration integrated classroom yoga module: A pilot study design.

BACKGROUND: The practice of yoga is proven to have physical, cognitive and emotional benefits for school children. Despite this many schools do not include yoga in their daily schedule. The reasons cited are lack of time and resources. To overcome these problems the present study aimed to develop and validate a short duration Integrated classroom yoga module. The design guidelines were that it should be possible to practice in the classroom environment and that it could be led by the class teacher. In this way the module would overcome the problem of both time and resource. MATERIALS AND METHODS: The study had two main phases. In the first phase, selected ICYM practices based on the literature review were validated by 21 subject matter experts using Lawhse's content validity ratio (CVR) formula. In the second phase, a pilot study using a paired sample prepost measurement design was carried out on 49 high school children. The study was conducted in June 2019. The intervention period was 1 month, and the test variables were physical fitness, cognitive performance, self-esteem, emotional well-being, and personality characteristic. Paired sample t-test was the analysis tool and the software used was the Statistical Package for the Social Science version 26. RESULTS: In the Lawshe's CVR analysis, 17 out of the 24 practices tested were rated by experts as essential as was the overall module (CVR score ≥0.429). In the pilot study, there were significant differences in the postmean scores compared to premean scores, for all the 4 EUROFIT physical fitness testing battery tests (P < 0.02), all the three scores of the Stroop color-word naming task (P < 0.001) and the Rosenberg self-esteem scale (P < 0.008). CONCLUSION: ICYM was validated and found feasible by the present study. It was found to have a statistically significant impact on physical fitness, cognitive performance, and self-esteem variables. However, a randomized control trial with a longer intervention period is needed to strengthen the present study.

Uncovering the molecular signature underlying the light intensity-dependent root development in Arabidopsis thaliana.

BACKGROUND: Root morphology is known to be affected by light quality, quantity and direction. Light signal is perceived at the shoot, translocated to roots through vasculature and further modulates the root development. Photoreceptors are differentially expressed in both shoot and root cells. The light irradiation to the root affects shoot morphology as well as whole plant development. The current work aims to understand the white light intensity dependent changes in root patterning and correlate that with the global gene expression profile. RESULTS: Different fluence of white light (WL) regulate overall root development via modulating the expression of a specific set of genes. Phytochrome A deficient Arabidopsis thaliana (phyA-211) showed shorter primary root compared to phytochrome B deficient (phyB-9) and wild type (WT) seedlings at a lower light intensity. However, at higher intensity, both mutants showed shorter primary root in comparison to WT. The lateral root number was observed to be lowest in phyA-211 at intensities of 38 and 75 µmol m - 2 s - 1. The number of adventitious roots was significantly lower in phyA-211 as compared to WT and phyB-9 under all light intensities tested. With the root phenotypic data, microarray was performed for four different intensities of WL light in WT. Here, we identified ~ 5243 differentially expressed genes (DEGs) under all light intensities. Gene ontology-based analysis indicated that different intensities of WL predominantly affect a subset of genes having catalytic activity and localized to the cytoplasm and membrane. Furthermore, when root is irradiated with different intensities of WL, several key genes involved in hormone, light signaling and clock-regulated pathways are differentially expressed. CONCLUSION: Using genome wide microarray-based approach, we have identified candidate genes in Arabidopsis root that responded to the changes in light intensities. Alteration in expression of genes such as PIF4, COL9, EPR1, CIP1, ARF18, ARR6, SAUR9, TOC1 etc. which are involved in light, hormone and clock pathway was validated by qRT-PCR. This indicates their potential role in light intensity mediated root development.

Impact of yoga on psychopathologies and quality of life in persons with HIV: A randomized controlled study.

BACKGROUND: Evidence suggests that individuals with human immunodeficiency virus (HIV) often exhibit poor physical and mental health, which contributes to a reduced Quality of Life (QoL). Yoga is a form of alternative therapy that has positive influences on general health and QoL. OBJECTIVES: This study examined the effects of yoga on i) anxiety, depression, and psychological well-being and ii) QoL among individuals with an HIV positive status. METHODOLOGY: Sixty individuals with HIV-positive (aged 30-50 years) from rehabilitation centres across Bangalore were randomly assigned to the yoga intervention group (n = 30; 11 men) or the wait-listed control group (n = 30; 10 men). Participants in the yoga group underwent 8 weeks of intense yoga practice performed an hour a day for 5 days a week. The yoga practice consisted of physical postures, breathing practices, relaxation techniques, and meditation. Participants in the wait-listed control group followed their normal routine. Anxiety, fatigue, depression, and QoL were assessed twice for all subjects in each group - once at the start of the study to establish a baseline and once more at the end of the 2-month study period to assess any changes. Data analysis was performed on the assessments using SPSS software version 10. RESULTS: Between group analysis demonstrated that a significantly different reduction in anxiety (p < 0.001), depression (p < 0.001), and fatigue (p < 0.001) was observed in the yoga group compared to the control group, as well as significant improvements in well-being (p < 0.001) and all domains of QoL (p < 0.001) in the yoga group compared to the control group. CONCLUSION: This study indicates that yoga intervention appears to improve the psychological health and QoL of individuals who are HIV-positive. Therefore, based on these findings, yoga may be recommended as a complementary therapy to enhance conventional HIV care.

Light and auxin signaling cross-talk programme root development in plants.

Root development in plants is affected by light and phytohormones. Different ranges of light wavelength influence root patterning in a particular manner. Red and white light promote overall root development, whereas blue light has both positive as well as negative role in these processes. Light-mediated root development primarily occurs through modulation of synthesis, signaling and transport of the phytohormone auxin. Auxin has been shown to play a critical role in root development. It is being well-understood that components of light and auxin signaling cross-talk with each other. However, the signaling network that can modulate the root development is an intense area of research. Currently, limited information is available about the interaction of these two signaling pathways. This review not only summarizes the current findings on how different quality and quantity of light affect various aspects of root development but also present the role of auxin in these developmental aspects starting from lower to higher plants.

Psychophysiological effects of yoga on stress in college students.

College students are vulnerable to a critical period in developmental maturation, facing rigorous academic work, and learning how to function independently. Physical activities such as running and bicycling have been shown to improve mood and relieve stress. However, college students often have low levels of physical activity. Yoga is an ancient physical and mental activity that affects mood and stress. However, studies examining the psychophysiological effects of yoga are rare in peer-reviewed journals. The aim of this study is to establish preliminary evidence for the psychophysiological effects of yoga on stress in young-adult college students. The present study suggests that yoga has positive effects on a psychophysiological level that leads to decreased levels of stress in college student. Further research is needed to examine the extent to which different types of yogic practices address the needs of different college subpopulations (e.g., overweight, sedentary, and smokers).

Effect of yoga practices in reducing counterproductive work behavior and its predictors.

CONTEXT: Aggression and negative affectivity (NA) are known for moderating relationship between job stressors and counterproductive work behavior. Yoga has been demonstrated earlier to reduce the parameters of aggression and negative emotions. AIM: The present study examined the effectiveness of yoga practices in reducing counterproductive work behavior and its predictors such as aggression and NA. SETTINGS AND DESIGN: A pretest-posttest randomized controlled design. MATERIALS AND METHODS: The study sample included yoga group (n = 80) and control group (n = 80). Yoga module that included asanas, pranayama, meditation, and yogic theory were taught to the yoga group. Mild to moderate physical exercises and management theories were taught to the control group. Both groups received intervention for 10 weeks, covering 1 h daily, 5 days a week. Measurements of self-reported counterproductive work behaviors, aggression, and NA were taken as baseline and postintervention for assessment. RESULTS: Yoga group reported significant reductions in aggression, NA, and counterproductive work behavior in comparison with the control group after 10 weeks. CONCLUSIONS: This study contributes by introducing a cost-effective way to prevent the heavy losses organizations are incurring due to counterproductive work behavior and its predictors. The findings support that yoga at workplace may result in positive psychological empowerment of the employees as well.

Well-being at workplace through mindfulness: Influence of Yoga practice on positive affect and aggression.

INTRODUCTION: Mindfulness is about being aware of internal and external stimuli by witnessing the act in a nonjudgmental manner. Earlier researches suggest that positive affectivity (PA) is negatively related to negative affectivity, aggression, and counterproductive work behavior (CWB). AIM: The present study examined the effect of mindfulness developed through Yoga practices on aggression and PA among working professionals involved in CWB. MATERIALS AND METHODS: A pre-test, post-test randomized controlled design was used with a study sample of Yoga group (n = 80) and control group (n = 80) for a duration of 10 weeks. Yoga module that included Asanas, Pranayama, meditation, and Yogic theories were taught to the Yoga group. Mild to moderate physical exercises and management theories were taught to the control group. Measurements of aggression and PA scores were taken at the baseline and postintervention for assessment. RESULTS: At the baseline, there was no significant difference in the variable scores between both the groups. Postintervention results revealed that Yoga group showed statistically significant (P < 0.001) reduction in aggression and significant (P < 0.001) enhancement in PA in comparison to the control group. CONCLUSIONS: When compared with the control group at the end of the intervention, the Yoga group scores were significantly lower for aggression and higher for PA.

Model of yoga intervention in industrial organizational psychology for counterproductive work behavior.

Counterproductive work behavior (CWB) has long been recognized as a broad spectrum of job behaviors and its link with negative affectivity and hostile behaviors. It is a major concern practically for all organizations. Repeated exposure to workplace stressor can result in a strain, an outcome of the job stress process that can be psychological, physical, or behavioral in nature, leading to CWBs. Yoga is a technique that brings an improvement on mental and physical level by means of posture, breathing control methods, and silencing the mind through meditation. Though yoga has received less scientific consideration, there has been a significant growth in the study of yoga in the healthy population. Mindfulness and self-control practices like yoga encourage individuals to be aware and accept their aggression linked thoughts and emotions simply as a short-lived state rather than to control them. The positive effects of yoga on the improvement of personality traits are already proven. This paper introduces a simple model of cost-effective, trials of yoga intervention at the workplace which could result in the twin benefits of substantial savings from losses for the employers by reducing the CWB and health improvements for the employees by reducing the negative affectivity and aggression. Internet databases such as PubMed, Google Scholar, and APA PsycNET were accessed. The available data were systematically reviewed in a structured manner and analyzed.