Efeito do diabetes associado ao envelhecimento na consolidação de fraturas na tíbia de ratos / Effect of diabetes associated to aging in the consolidation of fractures in the tibia of rats

Objetivo: Avaliar a qualidade histológica do calo ósseo de ratos idosos diabéticos após a produção de fratura exposta diafisária da tíbia. Métodos: Foram utilizados 20 ratos da linhagem Wistar (Rattus norvegicus, Rodentia mammalia), separados em dois grupos: 10 ratos idosos diabéticos (GID) e 10 ratos idosos normais (GIN). O diabetes foi induzido por injeção intraperitoneal de estreptozotocina (SZT) após 430 dias de vida. As fraturas expostas foram produzidas 150 dias após a indução pelo SZT, quando foram dosadas as glicemias e a hemoglobina glicosilada dos ratos. Após 21 dias, os ratos foram submetidos a eutanásia e as tíbias encaminhadas para avaliação histológica pelas colorações de hematoxilina-eosina e Alcian Blue. Resultados: Os ratos do grupo GIN apresentaram glicemias médias de 128,4mg/dl e os do grupo GID, 498,6mg/dl; as hemoglobinas glicosiladas demonstraram valor significantemente maior nos ratos GID. Histologicamente, nos calos ósseos foram observados predomínio de tecido cartilaginoso (70%) no GID e predomínio de tecido osteogênico (89%) no GIN. Não houve diferença entre os grupos quanto à quantidade de proteoglicanos. Conclusão: O diabetes mellitus induzido pela estreptozotocina retarda o processo de maturação do calo fraturário em ratos idosos.

Cross-cultural adaptation and validation of the Brazilian Portuguese version of the Neck Disability Index and Neck Pain and Disability Scale.

STUDY DESIGN: This study's design was a cross-cultural validation of the Neck Disability Index and Neck Pain and Disability Scale. OBJECTIVES: This study's objective was to translate, culturally adapt, and validate a Brazilian Portuguese version of the Neck Disability Index (NDI-BR) and the Neck Pain and Disability Scale (NPDS-BR). SUMMARY OF BACKGROUND DATA: Although several valid measures exist for measurement of neck pain and functional impairment, these measures have yet been validated in Brazilian Portuguese. Successful linguistic and cultural translation may allow appropriate cross-cultural comparison for clinical and laboratory research analysis. METHODS: The NDI-BR and NPAD-BR were culturally and linguistically translated from English into Brazilian Portuguese. The translated version of the instrument was administered to 203 patients at a midsize hospital in southern Brazil. Psychometric evaluation included factor analysis, internal reliability measures, test-retest reliability at 1 and 7 days, and criterion validity comparison with the Brazilian version of the SF-36. RESULTS: Factor analyses demonstrated a single-factor subscale for the NDI-BR and three subscales for the NPDS-BR. An item analysis showed a high degree of internal consistency for the NDI-BR (r = 0.74) and the three subscales of the NPDS-BR (subscale 1, r = 0.89; subscale 2, r = 0.81; subscale 3, r = 0.72). Test-retest reliability was also acceptable at for the NDI-BR (0.98 at baseline and 0.48 at 7 days) and subset one (0.96 at baseline and 0.91 at 7 days), subset 2 (0.96 at baseline and 0.62 at 7 days), and subset 3 (0.52 at baseline and 0.45 at 7 days) of the NPDS-BR. Construct validity was established during comparison of the Brazilian version of the SF-36. Only items associated with physical role, bodily pain, and emotional role failed significant correlation. CONCLUSIONS: A reliable and valid Portuguese version of the Neck Disability Index and Neck Pain and DisabilityScale was developed, which will facilitate the examination of functional performance within a large patient population, as well as cross-cultural comparisons.

Cross-cultural adaptation and validation of the Brazilian Portuguese version of the short musculoskeletal function assessment questionnaire: the SMFA-BR.

BACKGROUND: This study was designed to translate, culturally adapt, and validate a Brazilian Portuguese version of the Short Musculoskeletal Function Assessment Questionnaire (SMFA-BR). METHODS: The SMFA was translated from English into Brazilian Portuguese. Translations were synthesized, translated back into English, and then submitted to a committee of clinical, psychometric, and language experts. The questionnaire was then administered to 220 trauma patients at a midsize hospital in southern Brazil. Test-retest reliability was examined at one and seven days. Scale reliability and validity were assessed, and factor structure was analyzed. RESULTS: Patients with only one region of dysfunction reported less dysfunction than did patients with two or three regions of dysfunction. Both the SMFA-BR dysfunction and the SMFA-BR bother scores had significant correlations with all Brazilian Short Form-36 (SF-36) subscales except for bodily pain. Test-retest reliabilities, as determined by intraclass correlation analyses, were 0.99 (95% confidence interval, 0.97, 1.00) at one day (n = 10) and 0.99 (95% confidence interval, 0.96, 1.00) at seven days (n = 17) for the dysfunction index and 0.99 (95% confidence interval, 0.98, 1.00) at one day (n = 10) and 0.97 (95% confidence interval, 0.97, 1.00) at seven days (n = 17) for the bother index. Cronbach alpha reliabilities were 0.95 (95% confidence interval, 0.93, 0.97) and 0.91 (95% confidence interval, 0.89, 0.94) for the dysfunction and bother indices, respectively. Minimal ceiling and floor effects were observed for the bother subscale only. Exploratory factor analysis yielded a three-factor structure: upper-extremity dysfunction, lower-extremity dysfunction, and bother. CONCLUSIONS: A reliable and valid Brazilian Portuguese version of the SMFA was developed. It will facilitate the examination of functional performance within a large patient population as well as allow cross-cultural comparisons.

Avaliação funcional e histológica do reparo de nervo ciático utilizando cola de fibrina e sutura em ratos Wistar / Functional and histological assessment of sciatic nerve repair using fibrin glue and suture in Wistar rats

Foram avaliados os resultados do reparo da transecção aguda produzida no nervo ciático de ratos após a aplicação de cola de fibrina, em comparação com sutura epineural, e a associação entre sutura epineural e cola de fibrina. Utilizaram-se 30 ratos Wistar, separados em três grupos -S: sutura (n = 10); SC: sutura com dois pontos associada à cola de fibrina (n = 10); C: cola de fibrina (n = 10). Os animais foram avaliados pelo teste funcional do nervo ciático walking track, na '4 POT. a', '12 POT. a' e '16 POT. a' semanas e por histopatologia na '16 POT. a' semana. Os resultados funcionais foram comparados através da análise de variância (ANOVA), sendo adotado 0,05 como nível de rejeição da hipótese de nulidade. A avaliação funcional demonstrou resultados significantemente melhores no grupo SC (p < 0,05). Na 16ii semana observaram-se proliferação moderada de tecido conjuntivo no grupo S e leve nos grupos SC e C. A ocorrência de leve edema foi observada nos grupos SC e S e ausente no grupo C. Quanto à infiltração inflamatória, no grupo SC foi moderada, enquanto que nos grupos C e S foram leves. A sutura epineural associada à cola de fibrina no reparo da transecção aguda do nervo ciático de ratos proporcionou melhores resultados que o emprego de sutura e cola de fibrina isoladas