RESUMEN
We developed a method of clone proliferation promoting fusion genes whose proteins were activated by protein oligomerization through the helix-loop-helix region (PNT domain) of TEL. We inserted a cDNA library downstream of the PNT domain with a retrovirus vector. The resulting retrovirus infected cytokine-dependent 32D cells and cells with cytokine-independent growth were analyzed for the inserted cDNA. We cloned 25 independent fusion genes including seven kinds of partner genes. Six of the seven were a fusion of TEL with protein tyrosine kinase, LYN, HCK, FGR, SYK, FLT3, and TYK2. A serine/threonine kinase, ARAF1, was also found to fuse with TEL. These kinase fusion proteins included kinase domains with proper reading frames. These fusions may be a useful model for clarifying the downstream signal transduction of constitutive active kinase and this expression cloning method may provide a new tool with which to study cell proliferation signalling.
Asunto(s)
Clonación Molecular/métodos , Proteínas de Unión al ADN/genética , Proteínas de Unión al ADN/metabolismo , Células Mieloides/metabolismo , Proteínas Recombinantes de Fusión/biosíntesis , Proteínas Represoras/genética , Proteínas Represoras/metabolismo , Retroviridae/genética , Animales , División Celular/fisiología , Línea Celular , Transformación Celular Viral/genética , Dimerización , Regulación de la Expresión Génica/genética , Técnicas de Transferencia de Gen , Vectores Genéticos/genética , Ratones , Células Mieloides/citología , Polímeros/metabolismo , Proteínas Proto-Oncogénicas c-ets , Proteína ETS de Variante de Translocación 6RESUMEN
We measured serum magnesium (Mg) levels in 71 in-patients and out-patients with mood disorders and in 30 healthy controls and investigated the relationships between serum Mg levels and clinical background factors. Serum Mg levels were found to be significantly higher in patients with mood disorders than in controls. Serum Mg levels showed no significant correlation with patient sex, age, diagnosed subtype and disease phase in the mood disorder group. Serum Mg levels in patients with major depressive disorder who were taking psychotropic drugs were not significantly different from levels seen in patients with major depressive disorder who were not taking psychotropic drugs. These results suggest that the high serum Mg levels noted in patients with mood disorder are related to the underlying disorder itself and are not influenced by clinical background factors.
Asunto(s)
Magnesio/sangre , Trastornos del Humor/fisiopatología , Adulto , Trastorno Depresivo/fisiopatología , Femenino , Humanos , Masculino , Persona de Mediana Edad , Psicotrópicos/farmacología , Psicotrópicos/uso terapéuticoRESUMEN
A 42-year-old man was diagnosed as having refractory anemia in May, 2001. He developed overt leukemia and received allogeneic bone marrow transplantation (BMT). His younger brother, a 40-year-old man, was diagnosed as having acute leukemia with trilineage myelodysplasia in November, 2001. Although he was treated with conventional chemotherapy, he failed to achieve complete remission. He also received allogeneic BMT. We suggest that environmental factors in addition to a genetic defect in the pluripotent hematopoietic stem cells may be associated with the occurrence of this familial leukemia.