Discriminant analysis reveals differences in breed structure in river buffaloes of Maharashtra (India).

Buffaloes are a major milch species of India, and owning a true-to-breed buffalo herd is a reverie for livestock keepers, as it substantiates their incomes. Multivariate discriminate analysis is an efficient methodology to classify different farm animal populations based on canonical discriminant function. The present study was conducted on 392 adult buffaloes using 18 body biometric traits. Except for body height and body length, all the body characters revealed significant differences amongst the four buffalo populations. Unlike cattle, distance between horns and rump width were more discriminating factors, compared to body height, leg length, chest girth, and body length. We report the most suitable discriminate function which can discriminate with maximum between-group variation in four studied populations. Mahalanobis distances were calculated between populations and they ranged from 0.786 to 1.227. Purnathadi has revealed a distinct phenotypic structure, and these buffaloes have a distinct native breeding region comprising Akola, Amravati, and Buldhana districts of Maharashtra state. On the other hand, Elachpuri animals failed to depict physical and biometric discriminations from Nagpuri, Marathwadi, and Purnathadi buffalo populations, and Elachpuri can be considered as a strain of Nagpuri buffalo.

Role of herbal products in animal production - An updated review.

ETHNOPHARMACOLOGICAL RELEVANCE: Plants are used for health and medical functions since ancient times. Plants and their extracts are also well-known phytobiotics or phytogenics that are widely used in animal traditional and alternative medicine. In recent years the use of herbal/plant medicine is increased in livestock production due to the side effects of modern drugs, the high input costs, toxic residues in food, microbial resistance and due to the development of organic livestock production systems. Plants are used as health promoters and also for the treatment of diseases. AIM OF THE STUDY: This review aims to provide information on various plants used in animal health care, production and reproduction in various forms. METHODS: We carefully searched the scientific literature for data related to traditional scientific use of herbal products in animal production. This review summarized published literature collected from library, the online databases, and various scientific search engines including PubMed, Elsevier, Google Scholar, Science Direct, Scopus, and Research gate. The Plant List (www.theplantlist.org) databases were used to provide the scientific names, subspecies of plants. RESULTS: Various herbs play important role in production and reproduction of animal. Herbs acts as feed additives, growth promoters, immune boosters, improves reproduction in animals and also helps in reduction of methane and ammonia emission. Secondary metabolites like Tanins, saponin, flavonoids and essential oils are the most used for rumen manipulation and have great potential in poultry and pig nutrition. CONCLUSION: The traditional knowledge may play important role, hence documentation and preservation is a must before it is lost forever. Medicinal herbs are a potential source as therapeutics and nutritive aids and have a significant role in health system all over the world for both humans and animals.

Assessment of genetic diversity and bottleneck in Purnathadi buffaloes using short tandem repeat markers.

This study is the maiden attempt for genetic characterization of the Purnathadi, a phenotypically distinct buffalo population of the western Vidarbha region of Maharashtra and to explore genetic diversity using STR markers. A total of 48 unrelated Purnathadi buffaloes from the entire native tract were genotyped using a battery of 25 heterologous microsatellite markers. 5' end of forward primer of each microsatellite marker was labeled with one of the fluorescent dyes, viz., FAM (Blue), VIC (Green), NED (Yellow) or PET (Red) to assess the fragment length of genotyped PCR product with automated DNA sequencer (ABI 3100). 23 microsatellite loci (except ETH003 and ILSTS030) amplified successfully and adequately high allelic diversity (observed: 0.615 ± 0.043 and expected: 0.655 ± 0.037) was reported with 162 distinct microsatellite alleles. Sufficiently high Shannon index and PIC indicated the suitability of markers to evaluate genetic diversity in Purnathadi buffaloes. Within-population inbreeding estimates (FIS) for Purnathadi buffalo ranged between -0.171 and 0.495 with global FIS average of 5.9%. The outcome for IAM, TPM and test for mode shift revealed the absence of any recent bottleneck in Purnathadi buffalo. Pairwise FST (genetic differentiation) and gene flow between Purnathadi, Nagpuri and Marathwadi buffaloes were estimated using genotype data of 19 microsatellite markers. Lowest FST (0.031) was observed between Nagpuri and Purnathadi buffaloes with highest gene flow of 7.91% and highest FST (0.094) was between Purnathadi and Marathwadi populations. Present findings will definitely support in designing breeding plan for genetic improvement, as well as for developing conservation strategies of Purnathadi buffalo population. The comparative molecular study with other breeds of the regions is needed.