[Genetic-epidemiologic study of reproductive outcomes in the Ozersk population, located in "Maiak" control area]. / Genetiko-épidemiologicheskoe issledovanie reproduktivnykh iskhodov v populiatsii ozerska, raspolozhennogo v zone nabliudeniia po "Maiak".

In the population of Ozyorsk town situated in the control area of the nuclear enterprise (Production Association "Mayak") the number of pregnancies and its outcomes was studied in cohort of 2.258 women of completed fertility. The mean number of pregnancies per woman is 5.6 +/- 0.07. The mean size of family is 1.9 +/- 0.02. It was estimated that 2.7% of women had no pregnancies, 4.0% of women had no delivery till the end of their reproductive performance. 4.65% of women contributed nothing to the genofond of the next generation, i.e., they had not any children lived till their reproductive performance (20 years). The Crow Index of Opportunity for Selection and its components connected with differential fertility and differential mortality were estimated. In the population under study tow components of selection--selection at the prenatal stages (72.6%) and selection associated with infertility--are shown to be significant.

[Predisposition to multifactorial pathology in residents of the city in the zone around the "Maiak" atomic industry enterprise]. / Predraspolozhennost' k mul'tifaktorial'noi patologii u zhitelei goroda v zone predpriiatiia atomnoi promyshlennosti po "maiak".

According to present achievements in radiation and molecular genetics it is possible to expect that due to chronic radiation exposure the relative increasing of genetic risk in following generations will be observed. It will be due, in the first place, to increasing a percent of multifactorial diseases (MFD). Most of geneticists refer bronchial asthma (BA) to multifactorial diseases. Genetics and epidemiological analysis of liability to BA in population of Ozyorsk situated in the control area of "Mayak" Production Association (PA "Mayak") was accomplished. Population risk, as probability for an individual in population to develop BA up to the end of life is 2.69%. It's higher than in Moscow (2.03%) (p < 0.05). The excess is due to significantly higher values in females (2.96% against 1.93%). Population risk to fall ill of BA in females of Ozyorsk (2.96%) is significantly higher than in males (2.4%). Prevalence of BA in Ozyorsk (0.60%) is significantly higher than in Moscow (0.47%) due to higher values in females (0.65% against 0.47%). The total risk to fall ill of BA amongst relatives is 5.4% that is twice higher than the population risk. The heritability of BA is 0.71 (in Moscow-0.70).

[Professional chronic radiation exposure: prospects for studying mutagenic effects in generations using the Southern Ural DNA bank of irradiated people and their offspring]. / Professional'noe khronicheskoe radiatsionnoe vozdeistvie: perspektivy izucheniia mutationnykh éffektov v pokoleniiakh na osnove sozdannogo na iuzhnom Urale banka DNK obluchennykh liudei i ikh potomkov.

Data on the established DNA Bank were summarized. The DNA Bank included workers of the Mayak nuclear facility in the Southern Ural, who were exposed to chronic radiation predominantly from external gamma-rays in different doses, and their families for the future study on radiation mutations in somatic cells of parents and possible transmission of genome instability through the germline. At present the DNA Bank contains genetic material from 1500 individuals, among whom there are 223 families.

[Creation of an experimental cell culture vaccine against distemper using cell line 4647]. / Sozdanie éksperimental'noi kul'tural'noi vaktsiny protiv chumy plotoiadnykh s ispol'zovaniem kletok 4647.

Experiment in susceptible animals demonstrated the effectiveness and safety of several samples of a distemper vaccine prepared in cell line 4647. Preparations of the vaccine virus variants generated in line 4647 after 10 passages at 34 degrees C, 37 degrees C and 40 degrees C and 20 passages of rapidly multiplying virus (34 degrees C) possessed the protective activity when used for immunization in a dose of 1000 PFU/animal. The results obtained in observations of the animals and examinations of sera for antibodies did not differ from those of control tests of "Vakchum".

[Spontaneous sister chromatid exchanges in cultured African green monkey cells]. / Spontannye sestrinskie khromatidnye obmeny v kul'tiviruemykh kletkakh afrikanskoi zelenoi martyshki.

The frequencies of sister chromatid exchanges (SCE) were studied in primary kidney cell culture, diploid cell line 5018, pseudodiploid spontaneously transformed cell line 4647 and hypotetraploid cell line 455 of the African green monkey. Bromodeoxyuridine was used at a concentration of 10 micrograms/ml. The slides were stained with acridine orange and Giemsa for sister chromatid differentiation. In the first three cases, SCE were scored in 100 metaphases of each culture. The distribution of cells from the number of SCE differs from the normal law and was approximated fairly well by the lognormal distribution pattern. The geometrical means of SCE per cell were 6.90 for cells in primary culture, 9.26 for cells of diploid line 5018; 4.50 for cells of pseudodiploid transformed line 4647 and 9.76 for cells of hypotetraploid transformed line 455.