Changes in masseter muscle fibers by liquid diet rearing in rabbits and recovery by chewing of solid diet.

OBJECTIVE: To investigate the effects of liquid diet on the development of masseter muscle fibers and whether the changes in the masseter muscle can be recovered by chewing of solid diet. DESIGN: Masseter muscles from 40 rabbits (solid- and liquid-diet groups, n = 30; unweaned group, n = 5; recovery group, n = 5) were histochemically examined at 4, 12, 18, and 33 weeks after birth. Six fiber types (I, IC, IIC, IIA, IIAB, and IIB) were distinguished via mATPase staining. Muscle fiber diameter and fiber type composition were measured and compared between groups. RESULTS: In the liquid diet group, the diameter of types IIAB (solid group: 81.7 µm, liquid group: 60.9 µm) and IIB (solid group: 89.3 µm, liquid group: 68.8 µm) and the fiber type composition of type I (solid group: 18.4%, liquid group: 9.6%) decreased significantly at 33 weeks of age. In the recovery group, the fiber type composition of type I fibers recovered to 16.5%, while no recovery of type IIAB (56.6 µm) and IIB (64.6 µm) fiber diameter was observed. CONCLUSIONS: Liquid diet caused atrophy of muscle fibers and an increase in the proportion of fast-twitch fibers. Although the diameter and ratio of slow-twitch fibers were recovered by chewing of solid diet, recovery was not observed for fast-twitch fibers. Our findings are relevant for dental medicine as it explored the possibility of masticatory muscle function recovery by hard food.

Development of medial pterygoid muscle fibers in rabbits fed with a liquid diet.

OBJECTIVE: This study aimed to investigate the influence of decreased functional load on the medial pterygoid muscle during mastication in rabbits fed with a liquid-diet. MATERIALS AND METHODS: Medial pterygoid muscles from 54 rabbits (solid- and liquid-diet groups, n=48; unweaned group, n=6) were histochemically examined at 4, 9, 12, 18, and 33 weeks after birth. Six fiber types (I, IC, IIC, IIA, IIAB, and IIB) were distinguished via mATPase staining. RESULTS: Significant increases in the diameters of all fiber types were seen up to 33 weeks of age in the solid-diet group; however, no significant increase was noted in fiber types I and IC, from 4 to 33 weeks of age, in the liquid-diet group. The proportion of slow fibers increased up to 12 weeks followed by an increase in the number of fast fibers in the solid-diet group, whereas in the liquid-diet group, the number of slow fiber declined after weaning. CONCLUSIONS: Liquid-diet consumption caused muscle fiber atrophy and an increase in the number of fast fibers during early developmental stages after weaning. Furthermore, the growth pattern of the medial pterygoid muscle in the liquid-diet group was different from that in the solid-diet group.

Histochemical study of rabbit medial pterygoid muscle during postnatal development.

The medial pterygoid muscle is a layered structure like the masseter muscle. This study aimed at investigating the regional differences in fiber type composition and fiber diameter of the medial pterygoid muscle in the rabbit from birth until 33 weeks of age. Histochemical analysis of the medial pterygoid muscle was performed during five developmental stages (4, 9, 12, 18, and 33 weeks after birth) in 30 male Japanese white rabbits. Six fiber types (I, IC, IIC, IIA, IIAB, and IIB) were identified by mATPase staining. An increase in diameter was observed in fiber types I and IC until 9 weeks of age, and in fiber types IIC, IIA, IIAB, and IIB until 33 weeks of age. No significant differences in fiber diameter were noted in the different regions of the pterygoid muscle. Moderate fast to slow fiber type shifts occurred from weeks 4-12; thereafter, a rapid slow to fast fiber type shift was observed. Significant differences in fiber type composition based on regional differences were noted at 4 weeks of age. However, there was no difference in fiber type composition between regions at 33 weeks. In conclusion, it was clear that the diameter and proportion of fast fibers had increased even after reaching sexual maturity in rabbits. In addition, the medial pterygoid muscle tissues appeared to be homogenous at 33 weeks of age with very few differences between regions.