Histopathological and biochemical evaluation of paeoniflorin administration in an experimental periodontitis model.

The purpose of this study was to evaluate the effects of administered Paeoniflorin (Pae) on periodontal tissues within an experimental periodontitis model. Forty male Wistar rats were used in this study and experimental periodontitis was created in all rats except in the control group (n = 10, first group). In the periodontitis group, experimental periodontitis was created but no other application was performed (n = 10, second group). In the other groups created experimental periodontitis, systemic Pae (n = 10, third group) or saline (n = 10, fourth group) was applied. A biochemical analysis of the gingival vascular endothelial growth factor (VEGF) levels and a histomorphometric analysis (measurements of the area of alveolar bone, alveolar bone resorption, and attachment loss) were performed. In the Pae group, the area of the alveolar bone was increased, while alveolar bone resorption and attachment loss decreased. Gingival VEGF levels increased in all groups that created experimental periodontitis and the greatest increase seen in the Pae group. Histomorphometric and biochemical analyses in this study suggest that Pae has a curative effect on periodontal tissues. However, additional studies are needed to confirm these results.

Effect of Root Resection Length and Graft Type Used After Apical Resection: A Finite Element Study.

PURPOSE: Apical resection is a surgical technique that involves removing a tooth's root tip and sealing the apical extent of the root canal system. However, evidence-based information on the biomechanical effects of apically resected tooth determinants is lacking. The aim of the present study was to examine the biomechanical effects of using different graft materials and the effect of different resection quantities on the tooth radix. MATERIALS AND METHODS: Thirty finite element models of mandibular central incisors with 1-cm defects in their apical regions were constructed using SolidWorks software (SolidWorks, Waltham, MA). Resections of 0, 1, 2, 3, 4, and 5 mm were created at the root tips, and tissue regeneration was simulated in the empty cavity models using the material properties of healed bone, an allograft, a xenograft, and hydroxyapatite to the grafts used to fill the apical sites. A 100-N force was applied to the various groups of teeth in the oblique (45°) direction, and the maximal von Mises stress and displacement values were determined using Ansys, version 16, software (ANSYS, Inc, Canonsburg, PA). RESULTS: The highest of these values was observed in the 5-mm resected and empty model. Increasing the resection amount caused the stress and displacement to increase. The lowest stress and deformation values were seen in the hydroxyapatite group, followed by the xenograft group and the allograft group. CONCLUSIONS: The results of the present study have shown that grafting into the apical defect provides biomechanical support for resection of the root structure.

Histopathological and biochemical evaluation of the effect of Paeoniflorin on the periodontium during and after periodontitis formation in rats.

OBJECTIVES: Appraise the effect of systemic Paeoniflorin (Pae) application on the periodontium during and after induction of experimental periodontitis in the presence of ligature and after its removal. DESIGN: Seventy male Wistar rats were separated into seven equal groups. The first group was reserved as healthy control group (Group 1: no periodontitis, no medication) and experimental periodontitis was induced with ligature in the remaining rats. In experimental periodontitis groups, Pae or saline was administered systemically in three differet periods; during the experimental periodontitis induction (period 1), after experimental periodontitis induction which ligature removed (period 2) or ligature kept in position (period 3). Only, one of the groups acted as the control periodontitis group and received no treatment. Experimental periodontitis groups were as follows; Group 2: medication in period 1, Group 3: periodontitis and no medication, Group 4: medication in period 2, Group 5: saline application in period 2, Group 6: medication in period 3, Group 7: saline application in period 3. Matrix metalloproteinases-9 (MMP-9) levels and interleukin-10 (IL-10) levels were detected biochemically and histomorphometric analyses were performed. These analyses included measurements of the area of alveolar bone, the level of alveolar bone, and attachment loss. RESULTS: Area of alveolar bone and IL-10 levels were higher in the Pae-administered groups; level of alveolar bone, attachment loss, and MMP-9 levels were correspondingly lower (P < 0.05). The beneficial effects at histomorphometrical and biochemical levels of Pae were the strongest in the rats that were administered Pae after the removal of ligature. CONCLUSIONS: Systemically administered Pae had a positive effect on the healing of periodontal tissues. Pae can be used as a new therapeutic agent for periodontal diseases, but microbiology-based studies and more extensive biochemistry-based experimental and clinical studies are needed to address this possibility.

Apical surgery failures: Extraction or re-surgery? Report of five cases.

Apical surgery (AS) is considered as the last attempt to save teeth which cannot be treated with conventional endodontic approach. The main goal of apical surgery is to create a barrier between the root-canal system and the peri-radicular tissues by means of a tight root-end filling after resection. However, failures in this treatment is usually result with tooth loss. In such cases surgical re-treatment would take into consideration as viable alternative. In this case series, successful ARs that performed in ten teeth of five patients who applied for extraction after an unsuccessful apical surgery, were presented. It is pointed that if appropriate surgical and endodontic intervention is performed and adequate apical obturation is provided with retrograde filling, teeth can be treat without extraction.

Evaluation of the effects of Er:YAG laser, Nd:YAG laser, and two different desensitizers on dentin permeability: in vitro study.

The purpose of this in vitro study was to evaluate and compare the efficacy of erbium-doped yttrium aluminum garnet (Er:YAG) laser, neodymium-doped yttrium aluminum garnet (Nd:YAG) laser, PrevDent nano-hydroxyapatite toothpaste plus Repairing Serum Kit (PNH), and NUPRO Sensodyne Prophylaxis Paste with NovaMin (NPP) on dentin permeability reduction. Forty dentin discs obtained from bovine incisors were divided into four study groups: Er:YAG laser-treated (2940 nm; 0.2 W, 80 mJ/pulse, 3 Hz); Nd:YAG laser-treated (1064 nm; 1 W, 10 Hz); PNH-treated; and NPP-treated groups. The quantitative changes in permeability of each dentin disc were measured using a computerized fluid filtration method (CFFM) before and after desensitizer treatments. The data were analyzed using the Wilcoxon, paired-samples t, Kruskal-Wallis, and Mann-Whitney U tests. The dentin surfaces and tubules were also morphologically detected by scanning electron microscopy (SEM). In all groups, dentin permeability was significantly reduced after the desensitizer and laser treatments (p < 0.05). Among the groups, we detected a significant difference in only when comparing the Er:YAG laser- and NPP-treated groups (p = 0.034). SEM analysis revealed physical changes in the dentin surface in all groups. This in vitro study shows that all tested desensitizers and laser treatments reduced dentin permeability. Also, surface changes, such as complete or partial occlusion or shrinkage of dentin tubules, were observed in all groups. Although the laser groups performed best, the PNH protocol can be considered as an alternative therapeutic product. In addition, clinical and laboratory studies should be performed for this product, and their efficacy should be assessed by combined therapy with lasers.