RESUMEN
The role of metazoan coactivator SAYP in nuclear receptor-driven gene activation in the ecdysone cascade of Drosophila is considered. SAYP interacts with DHR3 nuclear receptor and activates the corresponding genes by recruiting the BTFly (Brahma and TFIID) coactivator supercomplex. The knockdown of SAYP leads to a decrease in the level of DHR3-activated transcription. DHR3 and SAYP interact during development and have multiple common targets across the genome.
Asunto(s)
Proteínas de Drosophila/metabolismo , Drosophila melanogaster/genética , Ecdisona/metabolismo , Regulación del Desarrollo de la Expresión Génica , Receptores Citoplasmáticos y Nucleares/metabolismo , Factores de Transcripción/metabolismo , Animales , Cromatina/genética , Cromosomas/genética , Proteínas de Drosophila/genética , Ecdisona/genética , Técnicas de Silenciamiento del Gen , Redes Reguladoras de Genes , Metamorfosis Biológica/genética , Receptores Citoplasmáticos y Nucleares/genética , Factores de Transcripción/genética , Activación TranscripcionalRESUMEN
In the S2 cell system of Drosophila melanogaster a key protein domain mediating the interaction of TFIID and Brahma transcriptional complexes into the BTFly supercomplex has been shown to be an evolutionary conserved SAY domain of the SAYP. TFIID and Brahma coactivators participated in the reporter gene activation induced by the SAY domain in cellular nuclei. The TFIID and Brahma components directly interacting with the SAY domain were identified.
Asunto(s)
Proteínas de Ciclo Celular/metabolismo , Proteínas de Drosophila/metabolismo , Complejos Multiproteicos/metabolismo , Transactivadores/metabolismo , Factor de Transcripción TFIID/metabolismo , Factores de Transcripción/metabolismo , Transcripción Genética/fisiología , Animales , Proteínas de Ciclo Celular/genética , Línea Celular , Núcleo Celular/genética , Núcleo Celular/metabolismo , Proteínas de Drosophila/genética , Drosophila melanogaster , Complejos Multiproteicos/genética , Estructura Terciaria de Proteína , Transactivadores/genética , Factor de Transcripción TFIID/genética , Factores de Transcripción/genéticaRESUMEN
SAYP is a dual-function transcriptional coactivator of RNA polymerase II. It is a metazoan-specific factor involved in different signaling pathways that control normal development. In Drosophila, SAYP is present in the organism from the early stages of development and participates in cell cycle synchronization at the blastoderm stage. SAYP is abundant in many embryonic cells and in imaginal discs of larvae and is crucial for oogenesis in adults. At the molecular level, SAYP serves as a basis for assembling the BTFly nuclear supercomplex consising of the Brahma and TFIID coactivators. We suppose that BTFly and other similar nuclear supercomplexes play an important role in ontogenesis.