RESUMEN
Allele frequency and forensic parameters for eleven STR loci were surveyed in a random sample from the Korean population.
Asunto(s)
Frecuencia de los Genes , Secuencias Repetidas en Tándem/genética , Humanos , Corea (Geográfico)RESUMEN
We have developed four multiplex genotyping systems (GeneKin Y-STR multiplexes) using silver staining with allelic ladders for ten Y-chromosome STR markers (DYS19, DYS385, DYS388, DYS389I/II, DYS390, DYS391, DYS392, DYS393 and DXYS156Y), with a view towards the application of rapid and simple genotyping assay methods for DNA profiling. The GeneKin Y-STR multiplexes developed have followed the published nomenclature and ISFG guidelines for STR analysis. Allele and haplotype frequencies at these Y-STRs loci were analysed by PCR amplification using the GeneKin Y-STR multiplexes, followed by denaturing polyacrylamide gel electrophoresis in 316 unrelated males in the Korean population. A total of 295 different haplotypes were found, 279 of them being unique. Gene diversity ranged from 0.4026 at DYS391 to 0.9606 at DYS385. The haplotype diversity value (which is the same as the discrimination index) calculated from all ten loci combined was 0.9995, which is informative. Our results revealed that a set of ten Y-STRs can discriminate between most of the male individuals in the Korean population (discrimination capacity: 93.35%). The Y-STR multiplexes thus provide useful information for forensic analysis and paternity tests and can also be of great benefit for providing information not normally available from autosomal DNA systems.
Asunto(s)
ADN/genética , Secuencias Repetidas en Tándem , Cromosoma Y , Alelos , Electroforesis en Gel de Poliacrilamida , Medicina Legal , Frecuencia de los Genes , Genotipo , Humanos , Corea (Geográfico) , Masculino , Paternidad , Reacción en Cadena de la PolimerasaRESUMEN
Allele and haplotype frequencies of seven Y-chromosome STR loci were determined from a sample of 330 unrelated males in the Korean population.
Asunto(s)
Pueblo Asiatico/genética , Dermatoglifia del ADN/métodos , Frecuencia de los Genes/genética , Haplotipos/genética , Repeticiones de Minisatélite/genética , Cromosoma Y/genética , Variación Genética/genética , Humanos , Corea (Geográfico) , Masculino , Datos de Secuencia Molecular , Reacción en Cadena de la Polimerasa/métodosRESUMEN
A patient with chronic lung disease and a right atrial density that was difficult to distinguish on a transthoracic echocardiogram underwent transesophageal echocardiography (TEE) that demonstrated two mobile masses attached to the anterior right atrial wall. During the TEE procedure, the patient experienced coughing and retching due to the esophageal intubation, and the embolization of one of the right atrial masses was observed. This case is the first to document this mechanism of pulmonary embolism (a mechanism that was suspected in two prior reports), and it questions the safety of procedures that induce retching and coughing in patients with mobile right atrial masses.
