A Descriptive Review on the Potential Use of Diatom Biosilica as a Powerful Functional Biomaterial: A Natural Drug Delivery System.

Although various chemically synthesized materials are essential in medicine, food, and agriculture, they can exert unexpected side effects on the environment and human health by releasing certain toxic chemicals. Therefore, eco-friendly and biocompatible biomaterials based on natural resources are being actively explored. Recently, biosilica derived from diatoms has attracted attention in various biomedical fields, including drug delivery systems (DDS), due to its uniform porous nano-pattern, hierarchical structure, and abundant silanol functional groups. Importantly, the structural characteristics of diatom biosilica improve the solubility of poorly soluble substances and enable sustained release of loaded drugs. Additionally, diatom biosilica predominantly comprises SiO2, has high biocompatibility, and can easily hybridize with other DDS platforms, including hydrogels and cationic DDS, owing to its strong negative charge and abundant silanol groups. This review explores the potential applications of various diatom biosilica-based DDS in various biomedical fields, with a particular focus on hybrid DDS utilizing them.

Recent Progress in Diatom Biosilica: A Natural Nanoporous Silica Material as Sustained Release Carrier.

A drug delivery system (DDS) is a useful technology that efficiently delivers a target drug to a patient's specific diseased tissue with minimal side effects. DDS is a convergence of several areas of study, comprising pharmacy, medicine, biotechnology, and chemistry fields. In the traditional pharmacological concept, developing drugs for disease treatment has been the primary research field of pharmacology. The significance of DDS in delivering drugs with optimal formulation to target areas to increase bioavailability and minimize side effects has been recently highlighted. In addition, since the burst release found in various DDS platforms can reduce drug delivery efficiency due to unpredictable drug loss, many recent DDS studies have focused on developing carriers with a sustained release. Among various drug carriers, mesoporous silica DDS (MS-DDS) is applied to various drug administration routes, based on its sustained releases, nanosized porous structures, and excellent solubility for poorly soluble drugs. However, the synthesized MS-DDS has caused complications such as toxicity in the body, long-term accumulation, and poor excretion ability owing to acid treatment-centered manufacturing methods. Therefore, biosilica obtained from diatoms, as a natural MS-DDS, has recently emerged as an alternative to synthesized MS-DDS. This natural silica carrier is an optimal DDS platform because culturing diatoms is easy, and the silica can be separated from diatoms using a simple treatment. In this review, we discuss the manufacturing methods and applications to various disease models based on the advantages of biosilica.

Environmental factors affecting akinete germination and resting cell awakening of two cyanobacteria.

Globally, cyanobacteria frequently cause blooms that outcompete other species in the waterbody, affecting the diversity, decreasing water exchange rates, and promoting eutrophication that leads to excessive algal growth. Here, Dolichospermum circinale (akinetes) and Microcystic aeruginosa (resting cells), were isolated from the sediment in the Uiam Dam in the North Han River and near Ugok Bridge in the Nakdong River, respectively. The morphology, germination process and rates, and growth was evaluated in different environmental conditions. D. cercinalis germination began on day two of culturing, with maximum cell growth observed on day ten. In contrast, M. aeruginosa exhibited daily increase in cell density and colony size, with notable density increase on day six. Next, different environmental conditions were assessed. Akinetes exhibited high germination rates at low light intensity (5-30 µmol/m2/s), whereas resting cells exhibited high growth rates at high light intensity (50-100 µmol/m2/s). Furthermore, both cell types exhibited optimum germination and growth in media containing N and P at 20-30° at a pH of 7-9. Our study reveals the optimum conditions for the germination and growth of cyanobacterial akinetes and resting cells isolated from river sediment, respectively, and will assist in predicting cyanobacterial blooms for appropriate management.

New recorded diatoms in Holocene sediment cores from the Gonggeom-ji Wetland in Korea.

The Gonggeom-ji reservoir is an agricultural one built for rice farming during the Proto-Three Kingdoms period and was designated as Gyeongsangbuk-do monument No. 121 because of its high historical value. The Nakdonggang National Institute of Biological Resources has been conducting paleontological and paleoenvironmental studies on major wetlands from Korea since 2016, as well as diatom, geological, and depth distribution analyses on the sedimentary soil of Gonggeom-ji. This study summarized the description and ecological characteristics of six newly recorded diatoms (Gomphonema lacusrankala, Pinnularia diandae, P. gibba var. hyaline, P. lacunarum, Sellaphora labda var. nipponica, Stauroneis angustilancea) found in samples collected through drilling in Gonggeom-ji in 2019.

Akinete germination chamber: An experimental device for cyanobacterial akinete germination and plankton emergence.

Understanding how algal resting cells (e.g. akinetes) germinate and what factors influence their germination rate is crucial for elucidating the development of algal blooms and their succession. While laboratory studies have demonstrated algal germination rate and some key factors affecting the germination, the use of artificially induced akinetes and/or removal of the sediments are obviously limiting in simulating the natural environment when designing such controlled experiments. This study introduce a laboratory Akinete Germination Chamber (AGC) that facilitates research for cyanobacterial akinete germination and emergence in an environment similar to natural conditions while minimizing sediment disturbance. The fundamental difference between AGC method and the conventional microplate method is that AGC incorporates the substrate from the natural environment whereas the microplate method does not employ sediment. Therefore, authors of this study assume that the characteristics of akinete germination between the two methods differ because the sediment influences the germination environment. The present study developed the AGC method as an efficient tool to understand harmful cyanobacterial bloom formation. For validation of the AGC method, this study evaluated akinete germination of Dolichospermum circinale (Anabaena circinalis) with different temperature and nutrient condition and then compared the results with those generated by conventional methods The results showed a marked difference in the maximal germination rate between two methods (78% and 35% in the AGC and the microplate, respectively; p < 0.05) at optimum germination temperature (25 °C for both the AGC and the microplate). The nutrient effect also demonstrated clear difference (p < 0.01) in the germination rate between two methods; 88%, 68% and 78% in the AGC and 15%, 20% and 15% in the microplate with -N+P, +N-P, and +N+P condition of CB medium, respectively. Importantly, both DW and -N-P treatments in the AGC induced a little germination of akinete (4.2 ±â€¯1.4% and 5.0 ±â€¯7.1%, respectively), whereas no germination was occurred in the DW treatment in the microplate, suggesting a possible positive effect of sediment on akinete germination. With these results, this study suspects that these differences were largely attributable to natural sediment. Also sediment-accompanied properties, possibly such as nutrient availability, heat budget, micronutrients, and bacteria might have some potential effects on akinete germination. The AGC method can overcome the limitations of the conventional microplate method, and that it is applicable in studies on pelagic-benthic coupling.