Six-wave mixing coherent anti-Stokes Raman scattering microscopy.

Acquiring images of biological tissues and cells without the assistance of exogenous labels with a fast repetition rate and chemical specificity is what coherent anti-Stokes Raman Scattering (CARS) imaging offers. Nonresonant background (NRB) is one of the main drawbacks of the CARS microscopy technique because it limits the detection of weak Raman lines and the detection of low-concentration molecules. We show that a six-wave mixing process with two beams, which is a cascade effect of CARS, show better signal/NRB ratio and can be utilized for biological tissues imaging. The cascade CARS (CCARS) depends on chi-3 to the fourth power, instead of chi-3 squared as in the usual CARS signal; therefore, the contrast ratio with NRB is higher for CCARS than for CARS. We present analytic calculations showing that CCARS have better contrast over CARS in any situation. Comparison of the signals of both techniques generated on water-ethanol solutions confirm these results. Finally, we acquired CCARS images of fresh biological tissues, attesting that it is a useful tool for biological studies.

Near-infrared transillumination of teeth: measurement of a system performance.

Transillumination (TI) of dental enamel with near-infrared light is a promising nonionizing imaging method for detection of early caries lesion. Increased mineral loss (caries lesion) leads to increased scattering and absorption. Caries thus appear as dark regions because less light reaches the detector. The aim of this work was to characterize the performance of a TI system from the resolution of acquired images using the modulation transfer function at two wavelengths, 1.28 and 1.4 mum. Test charts with various values of spatial periods, mimicking a perfect caries lesion, were attached to tooth sections, followed by capture of the transmitted image, using both wavelengths. The sections were then consecutively reduced in thickness, and a sequence of all sizes of the test charts were used for repeatedly imaging procedures. The results show that the TI system can detect feature size of 250 mum with 30% modulation. From the information about how the image degrades as it propagates through enamel, we also examined the possibility of estimating the position of a simulated approximal caries lesion by comparing images obtained from the two sides of a tooth section.

CMOS-compatible athermal silicon microring resonators.

We propose a new class of resonant silicon optical devices, consisting of a ring resonator coupled to a Mach-Zehnder interferometer, which is passively temperature compensated by tailoring the optical mode confinement in the waveguides. We demonstrate operation of the device over a wide temperature range of 80 degrees. The fundamental principle behind this work can be extended to other photonic devices based on resonators such as modulators, routers, switches and filters.

Characterization of enamel in primary teeth by optical coherence tomography for assessment of dental caries.

BACKGROUND: Caries is a disease that affects both primary and permanent dentitions, therefore new methods of caries diagnosis need to be tested on primary teeth as well as on permanent teeth. AIM: This study reports the application of optical coherence tomography (OCT) to characterize sound dental structure and detect natural caries of human primary teeth. DESIGN: Six primary teeth were sectioned into thin slices ( approximately 1.5 mm), and analysed perpendicular to the enamel surface by two home-made OCT systems operating around 1280 and 840 nm. The generated images were compared with histology as the gold standard. RESULTS: We demonstrated the efficacy of the OCT technique to measure the depth of the enamel layer, whose result was statistically compared with histology (P = 0.823; P > 0.05) with good agreement. We also determined, through measurement of contrast values, an increase in backscattered intensity of the order of two to three times between sound and caries regions. CONCLUSIONS: We employed OCT generated images to characterize the enamel layer. The technique showed great potential to be used on paediatric dentistry clinical on early caries detection with no pain, as it is a noninvasive method.

Sub-nm resolution cavity enhanced microspectrometer.

A novel on-chip spectrometer device using combined functionalities of a micro-ring resonator and a planar diffraction grating is proposed. We investigate the performance of this architecture by implementing it in a silicon-on-insulator platform. We experimentally demonstrate such a device with 100 channels, 0.1 nm channel spacing and a channel crosstalk less than -10 dB. The entire device occupies an area of less than 2 mm(2). Based on our initial results we envision that this device enables the possibility of the realization of low-cost and high-resolution ultra-compact spectroscopy.

In vitro tomographic image of human pulp-dentin complex: optical coherence tomography and histology.

INTRODUCTION: Optical coherence tomography (OCT), a noninvasive imaging method, was used in this work to image the pulp-dentin complex. Pulp and dentin are integrally connected in the sense that physiologic and pathologic reactions in one of the tissues will also affect the other. METHODS: An OCT system with 6-mum spatial resolution at 800 nm was used to image the pulp-dentin complex of in vitro samples. Five intact human maxillary premolars scheduled for surgical extraction were used in this in vitro study. The occlusal surfaces of teeth were polished with wet 600-, 1,000-, and 1,200-grit aluminum oxide abrasive paper perpendicular to the long axis of teeth, producing a plane on that surface. The images of pulp-dentin complex were taken by scanning the occlusal surface in a mesiodistal direction. The laser penetrated into the teeth structure and a tomographic image of pulp-dentin complex, parallel to the long axis of teeth, was obtained. Histological and OCT images were analyzed and compared. RESULTS: The results showed the capability of the OCT technique to generate images of the boundaries of pulp and its relation to the dentin. It could be clearly seen that the OCT image provided the insight into dentinal substrate about 0.65-mm deep (corrected for the dentin refractive index). CONCLUSION: OCT can be used in the future to prevent iatrogenic exposures of the pulp, complementing other existing methods, and will permit a more predictive prognosis of treatments.

In vitro imaging of remaining dentin and pulp chamber by optical coherence tomography: comparison between 850 and 1280 nm.

We report the application of optical coherence tomography (OCT) to generate images of the remaining dentin and pulp chamber of in vitro human teeth. Bidimensional images of remaining dentin and of the pulp chamber were obtained parallel to the long axis of the teeth, by two OCT systems operating around 1280 and 850 nm, and compared to tomography images using the i-CAT(R) Cone Beam Volumetric Tomography system as the gold standard. The results demonstrated the efficacy of the OCT technique; furthermore, the wavelength close to 1280 nm presented greater penetration depth in the dentine than 850 nm, as expected from scattering and absorption coefficients. The OCT technique has great potential to be used on clinical practice, preventing accidental exposure of the pulp and promoting preventive restoration treatment.

Evaluation of crack propagation in dental composites by optical coherence tomography.

OBJECTIVES: The purpose of this study was to image the sites of fracture initiation and slow crack propagation in a fiber reinforced composite, using the optical coherence tomography (OCT) technique. METHODS: Bar specimens (2mm x 3mm x 25mm) of fiber reinforced composite were mechanically and thermally cycled to emulate oral conditions. The interior of these samples was analyzed prior to and after loading, using OCT. The device used was a home-built Fourier domain OCT setup working at 800 nm with 6 microm spatial resolution. RESULTS: Intact specimens after load cycling were analyzed. It was clearly seen that OCT images provide an insight into crack propagation, which is not seen by the naked eye. SIGNIFICANCE: By using OCT the possibility of analyzing the fracture propagation quantitatively, and in depth, was added, opening up possibilities to quantitative studies.