Asunto(s)
Ciclosporina/uso terapéutico , Rechazo de Injerto/orina , Trasplante de Pulmón/fisiología , Tromboxano B2/orina , Análisis de Varianza , Animales , Biomarcadores/orina , Perros , Terapia de Inmunosupresión/métodos , Trasplante de Pulmón/inmunología , Radioinmunoensayo , Factores de Tiempo , Trasplante HomólogoRESUMEN
There is strong evidence indicating that the pancreas is under the influence of sex steroid hormones, and that it may even participate in their biosynthesis and metabolism. In the present study, [3H]testosterone was perfused into the isolated canine pancreas, and measured in the effluent with several of its metabolites (5 alpha-dihydrotestosterone, androstenedione, and estradiol). Results show that testosterone is readily transformed by the canine pancreas. The main product found in the effluent is androstenedione. The testis and spleen were also perfused with [3H]testosterone and used as controls. In both cases, this hormone appeared mostly unchanged in the effluent as compared to the pancreatic perfusion (p less than 0.0001). From our data, we conclude that the canine pancreas has the capacity to transform sex steroid hormones, and could be considered an extragonadal site of sex steroid biosynthesis.
Asunto(s)
Páncreas/metabolismo , Testosterona/metabolismo , Androstenodiona/metabolismo , Animales , Dihidrotestosterona/metabolismo , Perros , Estradiol/metabolismo , Técnicas In Vitro , Cinética , Masculino , Bazo/metabolismo , Testículo/metabolismo , TritioRESUMEN
The behavior of urinary thromboxane B2 (TXB2) during acute rejection of lung allotransplants was evaluated. Unmatched mongrel dogs were submitted to a left lung orthotopic allotransplantation (groups I and II), or a sham operation (group III). All animals had an initial significant elevation of TXB2 excretion due to surgical trauma; however, in sham-operated animals (group III) this elevation returned to basal levels after 3 days. All transplanted animals (groups I and II) had persistent TXB2 elevation with 2 important peaks on postop days 5 and 9. The elevated TXB2 excretion persisted in spite of immunosuppressive treatment with azathioprine and prednisone (group II). Rejection was followed by means of an objective grading system applied to chest roentgenograms taken on all animals. It was found that TXB2 levels correlated directly with the grade of radiographic changes seen, thus indicating degree of rejection. TXB2 can be useful as a noninvasive indicator for surveillance of lung allograft rejection.