Integrated multidisciplinary ecological analysis from the Uluzzian settlement at the Uluzzo C Rock Shelter, south-eastern Italy.

The Middle to Upper Palaeolithic transition, between 50 000 and 40 000 years ago, is a period of important ecological and cultural changes. In this framework, the Rock Shelter of Uluzzo C (Apulia, southern Italy) represents an important site due to Late Mousterian and Uluzzian evidence preserved in its stratigraphic sequence. Here, we present the results of a multidisciplinary analysis performed on the materials collected between 2016 and 2018 from the Uluzzian stratigraphic units (SUs) 3, 15 and 17. The analysis involved lithic technology, use-wear, zooarchaeology, ancient DNA of sediments and palaeoproteomics, completed by quartz single-grain optically stimulated luminescence dating of the cave sediments. The lithic assemblage is characterized by a volumetric production and a debitage with no or little management of the convexities (by using the bipolar technique), with the objective to produce bladelets and flakelets. The zooarchaeological study found evidence of butchery activity and of the possible exploitation of marine resources, while drawing a picture of a patchy landscape, composed of open forests and dry open environments surrounding the shelter. Ancient mitochondrial DNA from two mammalian taxa were recovered from the sediments. Preliminary zooarchaeology by mass spectrometry results are consistent with ancient DNA and zooarchaeological taxonomic information, while further palaeoproteomics investigations are ongoing. Our new data from the re-discovery of the Uluzzo C Rock Shelter represent an important contribution to better understand the meaning of the Uluzzian in the context of the Middle/Upper Palaeolithic transition in south-eastern Italy.

Rodents as indicators of the climatic conditions during the Middle Pleistocene in the southwestern Mediterranean region: implications for the environment in which hominins lived.

Rodents are a very useful tool in reconstructing the environment of the past, especially owing to their rapid response to climate change, their small home range, and their restricted habitat requirements. They are a highly diverse group of mammals, which have high reproduction rates and as a result can evolve rapidly. The abundance of their microfossil remains in archaeological and paleontological sites permits robust statistical analyses to reconstruct the past climate and environment. Recently, a number of studies have affirmed the need to deepen the climatic characterization of the European Quaternary, the Middle Pleistocene being an important stage for ascertaining how our hominin ancestors lived. The aim of this study is to characterize the climatic conditions in which hominins lived in southwestern Mediterranean Europe during the Middle Pleistocene. To reconstruct these climatic conditions, we apply the bioclimatic model to rodent assemblages from Middle Pleistocene sites with human remains (Caune de l'Arago, Sima de los Huesos, Aroeira cave, Visogliano, Trinchera Galeria, and Mollet cave). Based on the percentage distribution of the species in different climate types and applying multiple linear regressions, we estimated the mean annual temperature, the mean temperature of the coldest month, and the mean temperature of the warmest month. We compared these estimates with data collected over the last 30 years from nearby meteorological stations to obtain the differences with current climate and observe the fluctuations. The climatic conditions obtained from the results of this study show that, while in Iberia mild climatic condition prevailed, in southern France and northeastern Italy harsher weather conditions were indicated.

Understanding the emergence of modern humans and the disappearance of Neanderthals: Insights from Kaldar Cave (Khorramabad Valley, Western Iran).

Kaldar Cave is a key archaeological site that provides evidence of the Middle to Upper Palaeolithic transition in Iran. Excavations at the site in 2014-2015 led to the discovery of cultural remains generally associated with anatomically modern humans (AMHs) and evidence of a probable Neanderthal-made industry in the basal layers. Attempts have been made to establish a chronology for the site. These include four thermoluminescence (TL) dates for Layer 4, ranging from 23,100 ± 3300 to 29,400 ± 2300 BP, and three AMS radiocarbon dates from charcoal samples belonging to the lower part of the same layer, yielding ages of 38,650-36,750 cal BP, 44,200-42,350 cal BP, and 54,400-46,050 cal BP (all at the 95.4% confidence level). Kaldar Cave is the first well-stratified Late Palaeolithic locality to be excavated in the Zagros which is one of the earliest sites with cultural materials attributed to early AMHs in western Asia. It also offers an opportunity to study the technological differences between the Mousterian and the first Upper Palaeolithic lithic technologies as well as the human behaviour in the region. In this study, we present a detailed description of the newly excavated stratigraphy, quantified results from the lithic assemblages, preliminary faunal remains analyses, geochronologic data, taphonomic aspects, and an interpretation of the regional paleoenvironment.

Chronological and environmental context of the first hominin dispersal into Western Europe: The case of Barranco León (Guadix-Baza Basin, SE Spain).

The early Pleistocene site of Barranco León (Guadix-Baza Basin, SE Spain), dated to 1.4 Ma (millions of years ago), records the oldest hominin occurrence in Western Europe, as evidenced by the discovery of one tooth and thousands of Mode 1 stone tools. In this paper a detailed analysis of the microvertebrate content of the D1 and D2 units from this site is presented. The early Pleistocene in the Guadix-Baza Basin is characterized by a sharp climatic deterioration, which possibly impeded the settlement of this region by the early hominin population from the southern Caucasus. Shortly afterwards, when the climatic conditions were again favorable, a hominin presence is suddenly evidenced at the units D1 and D2 of Barranco León. According to the microvertebrate analysis of these units, the mean annual temperature at the time of deposition was significantly higher than 13 °C, with prevalent humid conditions. However, although most of the species were inhabitants of water edges, an open landscape was present in the vicinity of the lake. The data reported here clearly support the idea that the early hominin occupation of Europe was strongly constrained by climatic and environmental conditions, rather than by physiography or cultural factors.

Pleistocene history of Iberomys, an endangered endemic rodent from southwestern Europe.

The extant Cabrera's vole, Microtus cabrerae, differs in morphology and evolutionary history from the other species of Microtus. This arvicoline has unique derived features in the cranium, mandible and dentition. Probably its most conspicuous features are its large size, the high skull in lateral view, the long and distally broad nasals, and the triangle shape of the anteroconid complex, with a marked labio-lingual asymmetry of the occlusal surface of the first lower molars. In this study, we propose a phylogenetic lineage that includes Cabrera's vole in what until now has been the Microtus subgenus Iberomys. Paleontological information and several life history traits support the elevation of Iberomys to the rank of genus. Genus Iberomys comprises species that have appeared in succession during the Quaternary: in the Early Pleistocene, the extinct I. huescarensis in the Middle Pleistocene, the extinct I. mediterraneus and in the Late Pleistocene, the extant I. cabrerae. Interestingly, the extant species shows several biological singularities, such as multiple polymorphic copies of the SRY male-specific gene in both males and females, and the lowest basal metabolic rate in relation to weight among arvicoline species. Likewise, its habitat requirement is unique among the Iberian arvicolines. Accordingly, the biological and paleontological data that we present in this work support the elevation of its taxonomic rank to that of genus. This study also suggests a modification of nomenclature: Microtus (Iberomys) brecciensis is replaced with I. mediterraneus and the common name of the extant M. (I.) cabrerae changed from 'topillo' to 'iberon' to improve conservation and protection actions.

Climatic conditions for the last Neanderthals: Herpetofaunal record of Gorham's Cave, Gibraltar.

Gorham's Cave is located in the British territory of Gibraltar in the southernmost end of the Iberian Peninsula. Recent excavations, which began in 1997, have exposed an 18 m archaeological sequence that covered the last evidence of Neanderthal occupation and the first evidence of modern human occupation in the cave. By applying the Mutual Climatic Range method on the amphibian and reptile assemblages, we propose here new quantitative data on the terrestrial climatic conditions throughout the latest Pleistocene sequence of Gorham's Cave. In comparison with current climatic data, all mean annual temperatures were about 1.6-1.8 degrees C lower in this region. Winters were colder and summers were similar to today. Mean annual precipitation was slightly lower, but according to the Aridity Index of Gaussen there were only four dry months during the latest Pleistocene as opposed to five dry months today during the summer. The climate was Mediterranean and semi-arid (according to the Aridity Index of Dantin-Revenga) or semi-humid (according to the Aridity Index of Martonne). The atmospheric temperature range was higher during the latest Pleistocene, mainly due to lower winter temperatures. Such data support recent bioclimatic models, which indicate that high rainfall levels may have been a significant factor in the late survival of Neanderthal populations in southern Iberia. The Solutrean levels of Gorham's Cave and climate records from cores in the Alboran Sea indicate increasing aridity from Marine Isotope Stage (MIS) 3-2. Because Neanderthals seem to have been associated with woodland habitats, we propose that lessening rainfall may have caused the degradation of large areas of forest and may have made late surviving Neanderthal populations more vulnerable outside southern refuges like the Rock of Gibraltar.

Chronological and environmental context of the Middle Pleistocene human tooth from Mollet Cave (Serinyà, NE Iberian Peninsula).

Mollet Cave is a small cave situated in Serinyà (north-east Iberian Peninsula). It was excavated in 1947-48, 1958 and 1972 by Josep M. Corominas. An archaic human molar comes from its base layer (Layer 5). Up till now, this layer has only been dated based on a relative and imprecise chronology of macromammals and the archaeostratigraphic evidence from the early excavations. Recent excavations, conducted between 2001 and 2005, have made it possible to ascertain more precisely the archaeological and palaeontological contents of Mollet Cave, gather microvertebrates, and collect samples for radiometric dating. The aim of this paper is to present the absolute dating of Layer 5, as well as its palaeo environmental and climatic characterisation. The macromammal assemblage seems to have been the result of accumulations produced by the most abundant carnivore, the hyena, which would have used the cave as a den. The results obtained using uranium-series disequilibrium dating ascribe to Layer 5 an age of ca. 215 ka (thousands of years ago), which would correspond to MIS 7. The faunal association suggests a landscape formed by an open and humid woodland characteristic of an interstadial phase, which would have been an environment well suited to sustaining both hyenas and human groups.

Palaeoenvironment and palaeoclimate of the Mousterian-Aurignacian transition in northern Iberia: the small-vertebrate assemblage from Cueva del Conde (Santo Adriano, Asturias).

The transition from the Middle Palaeolithic (Mousterian) to the Upper Palaeolithic (Aurignacian) has been one of the prominent themes in the archaeology of the European Palaeolithic for more than 20 years. One of the most controversial questions concerning this period is the extinction of the Neanderthals and their replacement by modern humans. In this context, Cueva del Conde, located in the northern part of the Iberian Peninsula, is an archaeo-palaeontological site that records the Mousterian to Aurignacian transition. It has been excavated since the beginning of the 20th century, first by the Conde de la Vega del Sella and systematically by a team from the University of Oviedo since 2001. Three main zones have been identified: the External Zone, dated to approximately 39,110 ± 520 BP (level N104); the Entrance Platform, dated between 38,250 ± 390 BP and 34,730 ± 500 BP; and Gallery A with a radiocarbon date of approximately 31,540 ± 400 BP (level N2a2). The small-vertebrate assemblages recovered from the water-screening of all sediment from the excavation campaigns represent at least 21 small mammal, amphibian and squamate taxa. The small-vertebrate associations in the three zones suggest a patchy landscape, dominated by humid meadows and woodland areas with the existence of water in the vicinity of the cave. The climate shows a more continental pattern during the Mousterian, though it was milder during the Aurignacian. The small vertebrates of the Cueva del Conde Mousterian and Aurignacian levels suggest a climate that differed from modern day temperatures, between -1.1 and -4.4 °C (mean annual temperature), placing these assemblages during Interstadials 9 to 7 (Is9 to 7).

First fossil evidence of an "interglacial refugium" in the Pyrenean region.

A refugium is generally understood as an area where temperate species survive cold periods, such as the Iberian, Italian, or Balkan Peninsulas in Europe. Strictly speaking, this definition refers to what is known as a glacial refugium. However, there are various types of lesser-known refugia such as the interglacial refugium, which denotes a mountainous region at low latitudes, such as the Pyrenees, where species adapted to the cold survive during interstadial periods. The small-vertebrate association from the sequence of Cova Colomera, which is located on the southern face of the Pyrenees and contains the final cold spell of the Late Pleistocene and the beginnings of the temperate period in which we currently find ourselves (the Holocene), could constitute the first fossil evidence of such an interglacial refugium, thus providing new paleoecological data on the phenomenon.