RESUMEN
Safe and efficacious orally-delivered mucosal vaccine platforms are desperately needed to combat the plethora of mucosally transmitted pathogens. Lactobacillus spp. have emerged as attractive candidates to meet this need and are known to activate the host innate immune response in a species- and strain-specific manner. For selected bacterial isolates and mutants, we investigated the role of key innate immune pathways required for induction of innate and subsequent adaptive immune responses. Co-culture of murine macrophages with L. gasseri (strain NCK1785), L. acidophilus (strain NCFM), or NCFM-derived mutants-NCK2025 and NCK2031-elicited an M2b-like phenotype associated with TH2 skewing and immune regulatory function. For NCFM, this M2b phenotype was dependent on expression of lipoteichoic acid and S layer proteins. Through the use of macrophage genetic knockouts, we identified Toll-like receptor 2 (TLR2), the cytosolic nucleotide-binding oligomerization domain containing 2 (NOD2) receptor, and the inflammasome-associated caspase-1 as contributors to macrophage activation, with NOD2 cooperating with caspase-1 to induce inflammasome derived interleukin (IL)-1ß in a pyroptosis-independent fashion. Finally, utilizing an NCFM-based mucosal vaccine platform with surface expression of human immunodeficiency virus type 1 (HIV-1) Gag or membrane proximal external region (MPER), we demonstrated that NOD2 signaling is required for antigen-specific mucosal and systemic humoral responses. We show that lactobacilli differentially utilize innate immune pathways and highlight NOD2 as a key mediator of macrophage function and antigen-specific humoral responses to a Lactobacillus acidophilus mucosal vaccine platform.
Asunto(s)
Inmunidad Humoral/genética , Macrófagos/inmunología , Proteína Adaptadora de Señalización NOD2/genética , Vacunas/administración & dosificación , Administración Oral , Animales , Antígenos/administración & dosificación , Caspasa 1/genética , Caspasa 1/inmunología , Genes gag/genética , VIH-1/inmunología , VIH-1/patogenicidad , Humanos , Inmunidad Humoral/inmunología , Inflamasomas/genética , Inflamasomas/inmunología , Interleucina-1beta/genética , Interleucina-1beta/inmunología , Lactobacillus acidophilus/inmunología , Lipopolisacáridos/inmunología , Lipopolisacáridos/metabolismo , Macrófagos/microbiología , Ratones , Proteína Adaptadora de Señalización NOD2/inmunología , Ácidos Teicoicos/inmunología , Ácidos Teicoicos/metabolismo , Receptor Toll-Like 2/genética , Receptor Toll-Like 2/inmunología , Vacunas/inmunologíaRESUMEN
Display of heterologous antigens on the cell surface is considered a useful technique for vaccine delivery by recombinant lactobacilli. In this study, two recombinant Lactobacillus acidophilus derivatives displaying Salmonella flagellin (FliC) were constructed using different anchor motifs. In one instance, the FliC protein was fused to the C-terminal region of a cell envelope proteinase (PrtP) and was bound to the cell wall by electrostatic bonds. In the other case, the same antigen was conjugated to the anchor region of mucus binding protein (Mub) and was covalently associated with the cell wall by an LPXTG motif. These two recombinant L. acidophilus cell surface displays resulted in dissimilar maturation and cytokine production by human myeloid dendritic cells. The surface-associated antigen was highly sensitive to simulated gastric and small intestinal juices. By supplementation with bicarbonate buffer and soybean trypsin inhibitor, the cell surface antigen was protected from proteolytic enzymes during gastric challenge in vitro. The protective reagents also increased the viability of the L. acidophilus cells upon challenge with simulated digestive juices. These results demonstrate the importance of protecting cells and their surface-associated antigens during oral immunization.
Asunto(s)
Antígenos Bacterianos/metabolismo , Células Dendríticas/inmunología , Flagelina/metabolismo , Lactobacillus acidophilus/metabolismo , Vacunas contra la Salmonella/inmunología , Ácidos/metabolismo , Antígenos Bacterianos/genética , Células Cultivadas , Citocinas/metabolismo , Células Dendríticas/microbiología , Portadores de Fármacos , Estabilidad de Medicamentos , Flagelina/genética , Vectores Genéticos , Humanos , Lactobacillus acidophilus/genética , Péptido Hidrolasas/metabolismo , Proteínas Recombinantes/genética , Proteínas Recombinantes/metabolismo , Vacunas contra la Salmonella/genética , Vacunas Sintéticas/genética , Vacunas Sintéticas/inmunologíaRESUMEN
The enzyme-linked immunospot (ELISPOT) assay is a sensitive and relatively simple assay for detecting secreted cellular products such as cytokines and has become an invaluable immunological tool. The ELISPOT has been used extensively in human and murine research but has only recently been used to assess the feline immune system. For researchers studying feline disease or using the cat as a model of human disease, the quantification of cytokine-producing cells by ELISPOT is an invaluable technique for investigations of disease immunopathogenesis and vaccine efficacy. For example, use of the interferon (IFN)-gamma ELISPOT to measure the frequency of antigen-specific T-cells during feline immunodeficiency virus (FIV) infection or after immunization with candidate FIV vaccines is of particular interest. This application of the ELISPOT may serve to expand the utility of FIV as a model for human immunodeficiency virus. Broader applications of the ELISPOT should further our understanding of feline diseases and be useful in the rational development of more efficacious vaccines and therapeutic modalities for the enhancement of feline health. This chapter discusses important parameters of ELISPOT design that will enable researchers to develop and analyze the feline-specific assays within their own laboratory.
