History of the Athens Canadian Random Bred and the Athens Random Bred control populations.

The University of Georgia maintains two meat-type chicken control strains: the Athens Random Bred (ARB) and the Athens Canadian Random Bred (ACRB). The Athens Random Bred was developed from colored plumage commercial meat chicken strains in 1956. The ACRB is a replicate population of the Ottawa Meat Control strain which was developed in 1955 from white plumage commercial meat-type chickens. These genetic lines have been extremely valuable research resources and have been used extensively to provide comparative context to modern meat-type strains. The ACRB may be the oldest pedigreed control commercial meat-type chicken still in existence today. This paper reviews the history of the breed backgrounds for both control populations and reviews research utilizing the ACRB.

Mechanical harvesting of broilers.

At the end of the growout phase of broiler production essentially all broilers are caught and loaded into coops or cages entirely by hand. The catching of broilers remains one of the few aspects of broiler production not yet automated. Vacuum systems, permanent conveyors recessed in the floors of growout facilities, collecting mats, scoops, and self-propelled, combine-type devices have been tried with little success over the past 30 yr. Renewed efforts at developing mechanical broiler harvesters have been encouraging in recent years. Several equipment companies in North America and Europe have developed prototype mechanical harvesters that appear to have promise. The benefits of mechanical harvesting as compared to hand catching include lower costs and improved working conditions. In addition, studies conducted thus far indicate that mechanical harvesting will improve bird welfare both from a stress and injury standpoint.

The effect of triacylglycerol chain length on food intake in domestic fowl.

Triacylglycerols of varying chain lengths were tested for their effects on food intake in fasted Single-Comb White Leghorn cockerels 9-12 weeks of age. Tributyrin, tricaprylin, triolein, or saline were intubated intragastrically or infused intrahepatically. All triacylglycerol administrations were in 10 ml volumes with 10 kcal being infused intrahepatically and 10 or 20 kcal being infused intragastrically. When administered intragastrically, the short-chained triacylglycerol tributyrin decreased food intake at most times tested; the medium chain-length tricaprylin and the long chain-length triolein affected food intake sporadically. When infused into the liver, tributyrin and tricaprylin decreased food intake at most times tested, whereas triolein decreased food intake at 60 and 180 minutes only. Satiating effectiveness was related to triacylglycerol chain length when intubated, which may reflect rate of absorption from the gut; however, hepatic satiety receptors were also differentially sensitive to triacylglycerol chain length indicating that more than rate of assimilation from the gut must be considered.

Salt-soluble collagen and elastin in the human aorta and pulmonary artery.

Collagen and elastin, the major structural components of blood vessels, have a very low turnover. In disease, this rate may be increased and an elevation of the tissue concentration of the soluble degradation fragments might be anticipated. In this preliminary study the concentration of extractable collagen and elastin in the aorta and pulmonary artery of eight human subjects postmortem was determined. The proportion of pulmonary artery collagen and elastin that was soluble was generally either equal to or greater than that in the abdominal aorta. The fraction of collagen that was salt extractable was larger than the soluble elastin fraction.

Bovine inhibin immediately inhibits the electrophysiological response to chorionic gonadotrophin in ovarian follicles of Xenopus laevis.

Inhibin was originally isolated from ovarian tissue using, as a bioassay, its ability to diminish synthesis and secretion of gonadotrophins in pituitary cells after several days. Inhibin is known also to modify responses to gonadotrophins in the ovary after stimulation times of hours or days. Here we show that action for less than 4 min of purified bovine inhibin on ovarian follicles from Xenopus laevis causes a specific, dose-responsive (greater than 10 U/ml) inhibition of the membrane hyperpolarization produced by stimulation with chorionic gonadotrophin. The CG-induced change in follicle membrane potential has previously been shown to be caused by potassium efflux produced by rises in intracellular cAMP. Inhibin did not affect similar hyperpolarization stimulated by 10 microM adenosine. The apparent Ca(2+)-dependent Cl- currents which are often associated with CG action, were not inhibited by inhibin. At concentrations up to 2000 U/ml, inhibin did not significantly alter the timing of CG-induced germinal vesicle breakdown in the oocytes, which may suggest functional action on other developmental processes.

Angiotensin II stimulates an endogenous response in Xenopus laevis ovarian follicles.

While responses to angiotensin II have previously been induced in Xenopus laevis oocytes after injection of messenger RNA extracted from mammalian tissue, no endogenous responses of ovarian tissue to this hormone have been reported. Here we describe such an endogenous dose-dependent response to angiotensin II, detected by conventional electrophysiological techniques, in follicular oocytes. The ED50 of the response was estimated to be 0.15 +/- 0.07 microM (S.E.M.). Maximal depolarization, obtained at 1 microM angiotensin II, was 18.3 +/- 1.4 mV (n = 18, three experiments using oocytes from two toads, mean resting membrane potential = -42 +/- 2 mV). The response was absent from collagenase-treated oocytes or follicular oocytes treated with octanol, suggesting that the receptors are predominantly in the follicular layer surrounding the oocytes.

Food intake response of genetically selected high and low-weight line cockerels to plasma infusions from fasted fowl.

Two experiments were conducted to determine if food intake of free-feeding chickens could be affected by infusions of plasma from fasted fowl. In the first experiment, chickens from two lines genetically selected for high or low body weight were infused intrahepatically with plasma collected from free-fed and fasted individuals from each line. Food intake of low-weight line birds was increased significantly by infusions of plasma from fasted high-weight line chickens when compared to food intake of low-weight line birds receiving plasma from free-fed low-weight line chickens. Food consumption in high-weight line birds was unaffected by any of the plasma treatments. In the second experiment, plasma from free-fed and fasted high-weight line chickens was infused intrahepatically into Single Comb White Leghorn cockerels. Again the birds receiving the plasma from the fasted fowl consumed significantly more food than those infused with plasma from free-fed fowl. The results of these experiments suggest that some property of plasma from fasted birds stimulates appetite. Selection for increased body weight may have intensified this hunger factor in high weight fowl.

Avian cerebrospinal fluid: repeated collection and testing for a possible role in food intake regulation.

The objective of this study was to determine if a factor(s) involved in the regulation of food intake exists in the cerebrospinal fluid (CSF) of the domestic fowl. A technique was developed that allows blood-free CSF samples to be collected repeatedly from the cisterna magna for up to 6 weeks. Using this technique, CSF was collected from free-feeding and 24-hour fasted broiler cockerels and injected into the lateral cerebral ventricle of 8-week old Leghorn cockerels and 4-week old broiler cockerels. The free-feeding birds received 10 microliter intracerebroventricular injections of CSF either at normal concentration (1 X -CSF) (Experiments 1 and 3) or at four times normal concentration (4 X-CSF) (Experiments 2 and 4). Artificial CSF served as the control. Food and water consumption were monitored following injection. The 1 X- and 4 X-CSF collected from both the free-feeding and 24-hour fasted donors did not affect the food intake of either the Leghorn or the broiler recipients. Water intake, however, was significantly increased in the Leghorns and broilers receiving the 4 X-CSF collected from 24-hour fasted birds. Thus, there does not appear to be a factor(s) present in the CSF collected from the cisterna magna that alters food intake when injected into the lateral ventricle, but a factor may exist in the CSF collected from fasted donors that stimulates water intake.

Tryptophan's influence on feeding and body temperature in the fowl.

The neurotransmitter, serotonin, has been implicated in both food intake and body temperature regulation. In this experiment, the effect of tryptophan, the amino acid precursor of serotonin, on food consumption and rectal temperature in the fowl was determined. Single Comb White Leghorn cockerels were intubated with a tryptophan solution or distilled water. Tryptophan significantly (P less than or equal to .05) decreased both food intake and body temperature of the birds. These results suggest that tryptophan may inhibit food intake via peripheral rather than central mechanisms.

Intraperitoneal injections of tryptophan inhibit food intake in the fowl.

The effects of intraperitoneal injections of tryptophan on food intake in the chicken were examined. Four levels of tryptophan (12.5, 25, 50, 100 mg), and a saline control were tested in birds that were ad libitum fed or fasted 24 hr. The tryptophan treatments decreased food intake in the ad libitum-fed chickens in a dose-dependent manner. Food intake of the 24-hr fasted chickens was not influenced by the tryptophan injections. It is not known whether tryptophan is acting centrally or peripherally to decrease food intake of ad libitum-fed chickens.

Intraduodenal glucose infusions do not affect food intake in the fowl.

A series of experiments was performed to examine the role of the duodenum in food intake regulation in the fowl. In each of the experiments, birds were surgically fitted with intraduodenal cannulae. In Experiments 1 to 6, Single Comb White Leghorn (SCWL) or Rock Cornish cockerels were infused with isosmotic glucose, urea, or saline solutions. Glucose had no effect on food intake as compared with the saline and urea control treatments. In the final experiment, food intake of splanchnicectomized SCWL cockerels was contrasted to that of sham-operated controls, following intraduodenal glucose infusions. No difference between the denerved and intact birds was observed. The duodenum of the fowl, unlike the mammal, does not appear to play a significant role in food intake regulation.

Food intake in the domestic fowl: effect of intrahepatic lipid and amino acid infusions.

Five experiments were conducted to determine the effect of hepatic and jugular infusions of amino acid and lipid solutions on short-term food intake of cockerels. Neither jugular nor hepatic infusions of amino acids had any effect on food intake of Single-Comb White Leghorn (SCWL) cockerels. Lipid infusions had no effect on SCWL cockerels when administered intrajugularly but decreased food intake significantly when infused intrahepatically. In contrast, no effect on food intake was observed when Rock-Cornish (RC) cockerels were infused intrahepatically with lipid. Thus, it appears that the liver of the SCWL cockerel is not responsive to plasma amino acid concentrations but is sensitive to circulating lipid levels. This lipid sensitive mechanism may be important in the peripheral control of food intake in a chicken such as the SCWL which has been bred for egg production but may be desensitized in fowl such as the RC broiler which has been selected for large body size and feed consumption.

Reduced food intake following intracerebroventricular administration of a low molecular weight fraction of plasma from free-feeding fowl.

Plasma was collected from free-feeding Leghorn cockerels and partitioned by gel filtration into fractions of different molecular weight ranges. The individual fractions were then lyophilized and reconstituted to four times the original concentration. The plasma was administered to 10-week-old Leghorn cockerels via a stainless steel guide cannula, stereotaxically implanted into the lateral cerebral ventricle. Sated cockerels received 10 microliters intracerebroventricular (ICV) injections of one of the concentrated plasma fractions or of a control injection of artificial cerebrospinal fluid. Food and water intake were monitored following injection. Food intake was significantly decreased by the ICV injection of plasma fractions less than 1500 mol wt, whereas water consumption was not significantly different from that of the controls. The 1500 to 5000 mol wt fraction and the fraction above 5000 did not alter either food or water intake. These results suggest that the plasma of free-feeding domestic fowl contains a low molecular weight factor that is involved in the regulation of food intake.

Alteration of food intake following intracerebroventricular administration of plasma from free-feeding domestic fowl.

Four experiments were conducted to investigate the presence of a factor in the plasma of the domestic fowl that alters food intake when injected into the lateral cerebral ventricle. Plasma collected from free-feeding and 24-hour fasted birds was lyophilized and reconstituted to 2, 4, or 5 times normal concentration. Stainless steel guide cannulae were stereotaxically implanted into the lateral cerebral ventricle of 8-week-old Single Comb White Leghorn cockerels. Free-feeding birds received 10 microliter intracerebroventricular (ICV) injections of normal (Experiment 1) and concentrated (Experiments 2, 3, and 4) plasma collected from free-feeding and 24-hour fasted birds. Artificial cerebrospinal fluid (aCSF) served as the control. Food intake was significantly decreased by ICV injections of 2, 4, and 5 times normal concentration of plasma from fed birds. Plasma from fasted birds did not alter food intake regardless of concentration, but did significantly reduce water intake when concentrated to five times normal. It appears that a satiety factor, which can be detected by the central nervous system, is present in the plasma of free-feeding domestic fowl.

Food and water intake of broiler chicks as affected by intracerebroventricular injections of cholinomimetics.

The effects of intracerebroventricular (ICV) injections of the cholinomimetics, acetylcholine, methacholine, and carbachol on food and water intake in free-feeding broiler chicks were investigated. At 4 weeks of age, a 23-gauge, thin-walled stainless steel guide cannula was implanted stereotaxically into the right lateral ventricle of each bird. Following a minimum of 3 days to recover, the birds were injected with the various drugs. Acetylcholine and methacholine had no significant effect on food intake whereas carbachol significantly decreased food intake. Methacholine significantly increased water intake while acetylcholine and carbachol had no significant effect. These results suggest that cholinergic pathways within the central nervous system may be involved in the control of food and water intake in the domestic fowl.

Effect of intrahepatic glucose infusions on feeding in heavy and light breed chicks.

Although food intake regulation by the central nervous system in the fowl has received considerable attention, studies examining peripheral regulatory mechanisms are relatively few. The objective of these experiments was to determine the role of the liver in short-term regulation of feeding. Small volumes of isotonic glucose were infused into the hepatic circulation of undisturbed, unrestrained Single Comb White Leghorn (SCWL) and Rock-Cornish (RC) cockerels under both free-feeding and mildly-fasted conditions. Glucose infusions significantly decreased the food intake of free-feeding SCWL chicks but had no effect on mildly-fasted SCWL chicks. The RC chicks, whether free feeding or mildly fasted, were not influenced by the glucose infusions. These findings suggest that selection for increased growth rate has affected peripheral appetite regulatory mechanisms.

Effect of intracerebroventricular glucose injection on feeding in Leghorn chicks.

The food and water intake response of Leghorn chicks to intracerebroventricular (ICV) injections of glucose was studied. Experiments were done in both free-feeding and 24-hr fasted birds. The ICV injection of 10 microliter of glucose solutions ranging from 5.5 to 30% had no significant effect on food or water intake in free-feeding or in 24-hr fasted Leghorns. This suggests that glucose does not influence food or water intake when injected into the lateral cerebro-ventricle. This effect is discussed in relationship to recent findings showing that injection of glucose into the third ventricle decreases food intake.

Tyrosine and tryptophan influence on tonic immobility in the chicken.

The serotonergic and catecholaminergic systems have been shown to affect both tonic immobility and food intake in the domestic fowl. Tryptophan, the amino acid precursor of serotonin, has been demonstrated to influence tonic immobility and food intake when injected intraperitoneally. Tyrosine, amino acid precursor of the catecholamines, is also known to affect food intake when administered orally. In two experiments, tryptophan and tyrosine were intubated intragastrically into male chickens at levels known to affect food intake, and their effect on tonic immobility was measured. Tryptophan and tyrosine delivered intragastrically had no effect on either duration or susceptibility of tonic immobility. These data suggest that changes in appetite observed as a result of orally administered tyrosine and tryptophan were not due to a general inhibition or stimulation of the central nervous system.

Feeding, drinking and body temperature of Leghorn chicks: effects of ICV injections of biogenic amines.

The effects of dopamine (DA), norepinephrine (NE), epinephrine (E) and 5-hydroxytryptamine (5-HT) on food intake, water consumption and colonic body temperature (Tc) were investigated using Leghorn-type chicks. Food intake was not significantly affected by the intracerebroventricular injections of DA, NE or E. In contrast, 5-HT significantly decreased food intake in both sated and fasted chicks. Tc was significantly decreased by NE and E whereas DA and 5-HT prevented the decline seen in controls. Water consumption was not significantly affected by DA, NE or E; however, 5-HT increased water consumption of sated chicks and decreased water consumption in fasted ones. The feeding response of Leghorn chicks following ICV injection of these biogenic amines differs from that previously observed in the broiler-type chicks. This disparate response is discussed in relation to the different growth rates of the two types of birds.