Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 5 de 5
Filtrar
Más filtros










Base de datos
Intervalo de año de publicación
1.
J Appl Microbiol ; 130(3): 948-959, 2021 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-32866324

RESUMEN

AIMS: To identify the bacteria nodulating Sulla spinosissima growing profusely in a lead and zinc mine tailings in Eastern Morocco. METHODS AND RESULTS: In all, 32 rhizobial cultures, isolated from root nodules of S. spinosissima growing in soils of the mining site, were tolerant to different heavy metals. The ERIC-polymerase chain reaction (PCR) fingerprinting analysis clustered the isolates into seven different groups, and the analysis of the 16S rRNA sequences of four selected representative strains, showed they were related to different species of the genus Mesorhizobium. The atpD, glnII and recA housekeeping genes analysis confirmed the affiliation of the four representative strains to Mesorhizobium camelthorni CCNWXJ40-4T , with similarity percentages varying from 96·30 to 98·30%. The sequences of the nifH gene had 97·33-97·78% similarities with that of M. camelthorni CCNWXJ40-4T ; however, the nodC phylogeny of the four strains diverged from the type and other reference strains of M. camelthorni and formed a separated cluster. The four strains nodulate also Astragalus gombiformis and A. armatus but did not nodulate A. boeticus, Vachellia gummifera, Prosopis chilensis, Cicer arietinum, Lens culinaris, Medicago truncatula, Lupinus luteus or Phaseolus vulgaris. CONCLUSIONS: Based on similarities of the nodC symbiotic gene and differences in the host range, the strains isolated from S. spinosissima growing in soils of the Sidi Boubker mining site may form a different symbiovar within Mesorhizobium for which the name aridi is proposed. SIGNIFICANCE AND IMPACT OF THE STUDY: In this work, we show that strains of M. camelthorni species nodulating S. spinosissima in the arid area of Eastern Morocco constitute a distinct phylogenetic clade of nodulation genes; we named symbiovar aridi, which encompasses also mesorhizobia from other Mediterranean desert legumes.


Asunto(s)
Fabaceae/microbiología , Plomo/metabolismo , Mesorhizobium/fisiología , Minería , Simbiosis , Proteínas Bacterianas/genética , Genes Esenciales/genética , Especificidad del Huésped , Mesorhizobium/clasificación , Marruecos , Filogenia , Nodulación de la Raíz de la Planta/genética , ARN Ribosómico 16S/genética , Nódulos de las Raíces de las Plantas/microbiología , Microbiología del Suelo , Simbiosis/genética
2.
J Appl Microbiol ; 128(4): 1109-1118, 2020 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-31758847

RESUMEN

AIM: To analyse the diversity of nodule-forming bacteria isolated from Lupinus cosentinii naturally grown in the Maamora cork oak forest (Rabat, Morocco). METHODS AND RESULTS: Of the 31 bacterial strains, four were selected based on their REP-PCR fingerprinting that were studied by sequencing and phylogenetic analysis of their 16S rRNA, gyrB, dnaK, recA and rpoB housekeeping genes as well as the nodC symbiotic gene. The nearly complete 16S rRNA gene sequence of the four representative strains showed that they are related to Tunisian strains of genus Microvirga isolated from L. micranthus with nucleotide identity values ranging from 98·67 to 97·13%. The single and concatenated sequences of the 16S rRNA, gyrB, dnaK, recA and rpoB housekeeping genes indicated that the L. cosentinii-isolated strains had 99·2-99·9% similarities with the Tunisian L. micranthus microsymbionts. The nodC gene phylogeny revealed that the Moroccan strains clustered in the newly described mediterranense symbiovar, and nodulation tests showed that they nodulated not only L. cosentinii but also L. angustifolius, L. luteus and L. albus. CONCLUSIONS: To the best of our knowledge, this is the first report concerning the isolation, molecular identification and phylogenetic diversity of L. cosentinii nodule-forming endosymbionts and of their description as members of the Microvirga genus. SIGNIFICANCE AND IMPACT OF THE STUDY: In this work, we show that Microvirga sp. can be isolated from root nodules of wild-grown L. cosentinii in Northeast Africa, that selected strains also nodulate L. angustifolius, L. luteus and L. albus, and that they belong to symbiovar mediterranense. In addition, our data support that the ability of Microvirga to nodulate lupines could be related to the soil pH, its geographical distribution being more widespread than expected.


Asunto(s)
Lupinus/microbiología , Methylobacteriaceae/fisiología , Simbiosis , ADN Bacteriano/genética , Genes Esenciales/genética , Lupinus/clasificación , Methylobacteriaceae/clasificación , Methylobacteriaceae/genética , Methylobacteriaceae/aislamiento & purificación , Marruecos , Filogenia , ARN Ribosómico 16S/genética , Nódulos de las Raíces de las Plantas/microbiología , Análisis de Secuencia de ADN
3.
J Cereb Blood Flow Metab ; 32(3): 515-24, 2012 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-22086196

RESUMEN

Loss of plasma membrane integrity is a feature of acute cellular injury/death in vitro and in vivo. Plasmalemma-resealing agents are protective in acute central nervous system injury models, but their ability to reseal cell membranes in vivo has not been reported. Using a mouse controlled cortical impact (CCI) model, we found that propidium iodide-positive (PI+) cells pulse labeled at 6, 24, or 48 hours maintained a degenerative phenotype and disappeared from the injured brain by 7 days, suggesting that plasmalemma permeability is a biomarker of fatal cellular injury after CCI. Intravenous or intracerebroventricular administration of Kollidon VA64, poloxamer P188, or polyethylene glycol 8000 resealed injured cell membranes in vivo (P<0.05 versus vehicle or poloxamer P407). Kollidon VA64 (1 mmol/L, 500 µL) administered intravenously to mice 1 hour after CCI significantly reduced acute cellular degeneration, chronic brain tissue damage, brain edema, blood-brain barrier damage, and postinjury motor deficits (all P<0.05 versus vehicle). However, VA64 did not rescue pulse-labeled PI+ cells from eventual demise. We conclude that PI permeability within 48 hours of CCI is a biomarker of eventual cell death/loss. Kollidon VA64 reduces secondary damage after CCI by mechanisms other than or in addition to resealing permeable cells.


Asunto(s)
Lesiones Encefálicas/prevención & control , Permeabilidad de la Membrana Celular , Membrana Celular/efectos de los fármacos , Corteza Cerebral/patología , Pirrolidinas/uso terapéutico , Compuestos de Vinilo/uso terapéutico , Animales , Barrera Hematoencefálica/efectos de los fármacos , Barrera Hematoencefálica/metabolismo , Barrera Hematoencefálica/patología , Lesiones Encefálicas/patología , Lesiones Encefálicas/fisiopatología , Muerte Celular/efectos de los fármacos , Membrana Celular/patología , Corteza Cerebral/efectos de los fármacos , Corteza Cerebral/lesiones , Modelos Animales de Enfermedad , Inyecciones Intravenosas , Inyecciones Intraventriculares , Imagen por Resonancia Magnética , Masculino , Ratones , Actividad Motora/efectos de los fármacos , Actividad Motora/fisiología , Pirrolidinas/administración & dosificación , Compuestos de Vinilo/administración & dosificación
4.
J Cereb Blood Flow Metab ; 29(1): 87-97, 2009 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-18714331

RESUMEN

Earlier experiments have shown that cyclosporin A (CsA) and its non-calcineurin inhibitory analog NIM811 attenuate mitochondrial dysfunction after experimental traumatic brain injury (TBI). Presently, we compared the neuroprotective effects of previously determined mitochondrial protective doses of CsA (20 mg/kg intraperitoneally) and NIM811 (10 mg/kg intraperitoneally) when administered at 15 mins postinjury in preventing cytoskeletal (alpha-spectrin) degradation, neurodegeneration, and neurological dysfunction after severe (1.0 mm) controlled cortical impact (CCI) TBI in mice. In a first set of experiments, we analyzed calpain-mediated alpha-spectrin proteolysis at 24 h postinjury. Both NIM811 and CsA significantly attenuated the increased alpha-spectrin breakdown products observed in vehicle-treated animals (P<0.005). In a second set of experiments, treatment of animals with either NIM811 or CsA at 15 mins and again at 24 h postinjury attenuated motor function impairment at 48 h and 7 days (P<0.005) and neurodegeneration at 7 days postinjury (P<0.0001). Delayed administration of NIM811 out to 12 h was still able to significantly reduce alpha-spectrin degradation. These results show that the neuroprotective mechanism of CsA involves maintenance of mitochondrial integrity and that calcineurin inhibition plays little or no role because the non-calcineurin inhibitory analog, NIM811, is as effective as CsA.


Asunto(s)
Lesiones Encefálicas/prevención & control , Ciclosporina/química , Ciclosporina/farmacología , Fármacos Neuroprotectores/farmacología , Animales , Lesiones Encefálicas/metabolismo , Lesiones Encefálicas/patología , Calpaína/metabolismo , Citoesqueleto/efectos de los fármacos , Citoesqueleto/enzimología , Inmunosupresores/química , Inmunosupresores/farmacología , Masculino , Ratones , Actividad Motora/efectos de los fármacos , Fármacos Neuroprotectores/química , Condicionamiento Físico Animal , Espectrina/metabolismo
5.
J Cereb Blood Flow Metab ; 26(11): 1407-18, 2006 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-16538231

RESUMEN

In the present study, we investigate the hypothesis that mitochondrial oxidative damage and dysfunction precede the onset of neuronal loss after controlled cortical impact traumatic brain injury (TBI) in mice. Accordingly, we evaluated the time course of post-traumatic mitochondrial dysfunction in the injured cortex and hippocampus at 30 mins, 1, 3, 6, 12, 24, 48, and 72 h after severe TBI. A significant decrease in the coupling of the electron transport system with oxidative phosphorylation was observed as early as 30 mins after injury, followed by a recovery to baseline at 1 h after injury. A statistically significant (P<0.0001) decline in the respiratory control ratio was noted at 3 h, which persisted at all subsequent time-points up to 72 h after injury in both cortical and hippocampal mitochondria. Structural damage seen in purified cortical mitochondria included severely swollen mitochondria, a disruption of the cristae and rupture of outer membranes, indicative of mitochondrial permeability transition. Consistent with this finding, cortical mitochondrial calcium-buffering capacity was severely compromised by 3 h after injury, and accompanied by significant increases in mitochondrial protein oxidation and lipid peroxidation. A possible causative role for reactive nitrogen species was suggested by the rapid increase in cortical mitochondrial 3-nitrotyrosine levels shown as early as 30 mins after injury. These findings indicate that post-traumatic oxidative lipid and protein damage, mediated in part by peroxynitrite, occurs in mitochondria with concomitant ultrastructural damage and impairment of mitochondrial bioenergetics. The data also indicate that compounds which specifically scavenge peroxynitrite (ONOO(-)) or ONOO(-)-derived radicals (e.g. ONOO(-)+H(+) --> ONOOH --> (*)NO(2)+(*)OH) may be particularly effective for the treatment of TBI, although the therapeutic window for this neuroprotective approach might only be 3 h.


Asunto(s)
Hemorragia Encefálica Traumática/tratamiento farmacológico , Hemorragia Encefálica Traumática/patología , Mitocondrias/patología , Fármacos Neuroprotectores/uso terapéutico , Estrés Oxidativo/efectos de los fármacos , Animales , Northern Blotting , Hemorragia Encefálica Traumática/metabolismo , Calpaína/fisiología , Citoesqueleto/patología , Masculino , Lípidos de la Membrana/metabolismo , Proteínas de la Membrana/metabolismo , Ratones , Microscopía Electrónica , Mitocondrias/metabolismo , Degeneración Nerviosa , Consumo de Oxígeno/fisiología , Ácido Peroxinitroso/metabolismo , Especies Reactivas de Oxígeno , Tirosina/análogos & derivados , Tirosina/metabolismo
SELECCIÓN DE REFERENCIAS
DETALLE DE LA BÚSQUEDA
...