Effect of prefabricated immediate interim prosthesis design and insertion workflow on seating accuracy on implants placed via static computer-assisted implant surgery: A cross-sectional in vitro study.

BACKGROUND: Immediate implant restoration by prefabricated prosthesis has multiple benefits. However, the design and insertion workflow of the prosthesis may influence the seating. PURPOSE: Evaluation of seating accuracy of prefabricated interim prosthesis of different designs and insertion workflows for immediate restoration of implants placed via static computer-assisted implant surgery (sCAIS). MATERIALS AND METHODS: A maxillary model without incisors was used to plan for two implants at the lateral incisor locations. According to the planned implants, sCAIS surgical template and a four-unit interim prosthesis were designed. Four prostheses were fabricated based on the design and insertion workflow. The first prosthesis involved complete fabrication (CF) of the interim prosthesis, where the interim prosthesis is fabricated for laboratory attachment to abutments. The other three prostheses were produced by partial fabrication (PF), where the interim prosthesis shell was produced with internal spacing between the fitting surface and the abutments. The PF prostheses were cemented on abutments attached to the inserted implants. Three different PF prosthesis designs were included with different levels of internal spacing: 100 µm (PF.1), 200 µm (PF.2), and 300 µm (PF.3). A total of 15 surgical models received implants on which each prosthesis was seated and scanned by a laboratory scanner. The vertical, horizontal, and proximal contact errors were measured. RESULTS: Although all prostheses were seated on every model, the CF prostheses had greater vertical error, followed by PF.1, PF.2, and PF.3 prostheses, respectively. A similar pattern was observed for proximal contact error, where PF.3 was most superior. PF.3 prostheses had the least horizontal error than the other prostheses. CONCLUSIONS: All interim prostheses experienced errors at the vertical, horizontal, and proximal surfaces, which can be attributed to deviations of the inserted implants. The PF of interim prosthesis with increased internal spacing for intraoral insertion appeared to reduce seating errors.

Citrus Bright Spot Virus: A New Dichorhavirus, Transmitted by Brevipalpus azores, Causing Citrus Leprosis Disease in Brazil.

Citrus leprosis (CL) is the main viral disease affecting the Brazilian citriculture. Sweet orange (Citrus sinensis L. Osbeck) trees affected by CL were identified in small orchards in Southern Brazil. Rod-like particles of 40 × 100 nm and electron lucent viroplasm were observed in the nucleus of infected cells in symptomatic tissues. RNA extracts from three plants, which proved negative by RT-PCR for known CL-causing viruses, were analyzed by high throughput sequencing and Sanger sequencing after RT-PCR. The genomes of bi-segmented ss(-)RNA viruses, with ORFs in a typical organization of members of the genus Dichorhavirus, were recovered. These genomes shared 98-99% nt sequence identity among them but <73% with those of known dichorhavirids, a value below the threshold for new species demarcation within that genus. Phylogenetically, the three haplotypes of the new virus called citrus bright spot virus (CiBSV) are clustered with citrus leprosis virus N, which is a dichorhavirus transmitted by Brevipalpus phoenicis sensu stricto. In CiBSV-infected citrus plants, B. papayensis and B. azores were found, but the virus could only be transmitted to Arabidopsis plants by B. azores. The study provides the first evidence of the role of B. azores as a viral vector and supports the assignment of CiBSV to the tentative new species Dichorhavirus australis.

Historical and Contemporary Perspectives on the Biological Control of Aphids on Winter Cereals by Parasitoids in South America.

Aphids are worldwide pests, and in South America, they harm many crops including winter cereals. In the 1970s, the rapid expansion of the wheat crop area in the subtropical region of South America led to growth of aphid populations. The wide availability of food, associated with the low effectiveness of natural biological control, put the aphid populations out of balance, requiring intensive use of insecticides. At the end of the decade, biological control programs of aphids were initiated in Argentina, Brazil, and Chile, including the importation of natural enemies (mainly parasitoids), followed by their laboratory rearing and field release. With decreased use of highly hazardous pesticides, biological control by introduced and already-present parasitoid species was enhanced. The program was very successful and aphid populations have been kept at low levels. This review article explores the history of this program and its current status. In modern day agriculture, with intense multiple cropping systems, adoption of several conservation practices, and increased cultivation of wheat in tropical regions, we discuss ways to keep this program effective to maintain aphid populations on cereal crops at low acceptable levels through employing biological control agents.

Proximal contact loss between implant prostheses and adjacent natural teeth: A qualitative systematic review of prevalence, influencing factors and implications.

Objectives: To evaluate the prevalence of proximal contact loss (PCL) between implant prostheses and natural teeth, and identify the risk factors and implications associated with PCL. Data/sources: PubMed (MEDLINE), Google Scholar, Cochrane Library Database, Scopus, EMBASE, Open Grey, ScienceDirect, and Web of Science were electronically searched to retrieve clinical studies on PCL around implant prostheses up to September 2021. Study selection: A total of 19 studies were eligible. The short-term studies (less than 2 years) revealed a PCL prevalence of 11-30%, the medium-term studies (2-5 years) indicated a PCL prevalence of 13-65%, and the long-term studies (more than 5 years) showed a PCL prevalence of 29-83.3%. The likely influencing factors were the duration of service and the mesial location of proximal contacts. Other factors, such as occlusion, vitality of adjacent teeth, implant location, patient age and splinting had a less obvious relation to PCL. The reported implications of PCL were food impaction and patient dissatisfaction. Bone loss, peri-implant inflammation, bleeding on probing and pocket depth had a less clear association with PCL. Conclusions: PCL development between implant prostheses and natural teeth is frequent, inevitable and progressive. While the review identified several influencing factors and implications of PCL, future research is needed to outline the influence of prosthesis design on PCL and food impaction. Clinical significance: Patients with implant prostheses should be informed about PCL likelihood and the risk of food impaction around implant prostheses. The proximal contact quality and its implications should be monitored during the review visits.

A novel tenuivirus infecting wheat in Brazil.

Since 1948, pale yellow wheat spike have been reported in southern Brazil. This symptom was associated with tenuiviruses due to the observation of cytoplasmic inclusions constituted by a mass of filamentous particles (7-10 nm in diameter) with indeterminate length, identical to those found in "leaf dip" preparations. Such symptoms are still seen in wheat crops; however, there is a lack of information regarding this pathosystem. Decades after the first report, the first sequences of wheat white spike virus were characterized. Wheat plants with symptoms such as pale yellowing, chlorotic streaks, and leaf mosaic were collected in Paraná State, Southern Brazil. High-throughput sequencing was used to determine the nearly complete nucleotide sequence of the viral genome. The genome is composed of five RNAs with a total size of 18,129 nucleotides, with eight open reading frames (ORFs). The virus identified in this study can be included in a new species in the family Phenuiviridae, genus Tenuivirus, and we have tentatively named this virus "wheat white spike virus".

Oscillation, synchrony, and multi-factor patterns between cereal aphids and parasitoid populations in southern Brazil.

In different parts of the world, aphid populations and their natural enemies are influenced by landscapes and climate. In the Neotropical region, few long-term studies have been conducted, maintaining a gap for comprehension of the effect of meteorological variables on aphid population patterns and their parasitoids in field conditions. This study describes the general patterns of oscillation in cereal winged aphids and their parasitoids, selecting meteorological variables and evaluating their effects on these insects. Aphids exhibit two annual peaks, one in summer-fall transition and the other in winter-spring transition. For parasitoids, the highest annual peak takes place during winter and a second peak occurs in winter-spring transition. Temperature was the principal meteorological regulator of population fluctuation in winged aphids and parasitoids during the year. The favorable temperature range is not the same for aphids and parasitoids. For aphids, temperature increase resulted in population growth, with maximum positive effect at 25°C. Temperature also positively influenced parasitoid populations, but the growth was asymptotic around 20°C. Although rainfall showed no regulatory function on aphid seasonality, it influenced the final number of insects over the year. The response of aphids and parasitoids to temperature has implications for trophic compatibility and regulation of their populations. Such functions should be taken into account in predictive models.

Duration, deviation and operator's perception of static computer assisted implant placements by inexperienced clinicians.

INTRODUCTION: This study measured the duration, deviation and operator's perception of implant placement by fully guided (FG), pilot-guided (PG) and freehand (FH) protocols by postgraduate students with minimal implant experience. MATERIALS AND METHODS: Twenty postgraduate students participated in the study. Half of them placed single anterior (S-Ant) and single posterior (S-Post) implants, and the other half placed anterior (B-Ant) and posterior (B-Post) implants in a wide edentulous area. The PG placement involved surgical guides that only controlled pilot drilling, whilst the FG placement controlled all the drilling steps and implant placement. The duration of implant placement and the operator's perception (ease of drilling, ease of implant placement and operator's preference) were measured. The deviations of placed implants were quantified by measuring the trueness and angulation deviations in relation to the planned implants. RESULTS: The PG placement was the quickest for inserting implants, followed by FG and FH placements, respectively (p < .05). The location of the implant had influenced the duration of implant placement only for the PG placement. In relation to ease of drilling, ease of implant placement and operator's preference, there was no significant difference amongst the different placement protocols or implant locations. The FG placement was associated with least deviations, followed by PG and FH placements, respectively (p < .05). CONCLUSIONS: In the hands of postgraduate students with minimal implant experience, FG and PG placements reduced the implant placement duration in comparison with FH placement. The FG placement was consistently more accurate followed by PG placement.

Sequential Post-Heading Applications for Controlling Wheat Blast: A 9-Year Summary of Fungicide Performance in Brazil.

Wheat blast, caused by Pyricularia oryzae Triticum lineage, is a major constraint to wheat production, mainly in the tropics of Brazil, where severe epidemics have been more frequent. We analyzed disease and wheat yield data from 42 uniform field trials conducted over 9 years (2012 to 2020) to assess whether the percent control and yield response were influenced by fungicide type, region (tropical or subtropical), and year. Six treatments were selected, all evaluated in at least 19 trials. Two fungicides were applied as solo active ingredients (MANCozeb, and TEBUconazole), and four were premixes (AZOXystrobin plus TEBU, TriFLoXystrobin plus PROThioconazole, TFLX plus TEBU, and PYRAclostrobin plus EPOXiconazole). Percent control, calculated from back-transforming estimates by a meta-analysis network model fitted to the log of the means, ranged from 43 to 58%, with all but PYRA plus EPOX showing efficacy >52% on average, not differing among them. The variation in both efficacy and yield response was explained by region, and all but TEBU performed better in the subtropics than in the tropics. Yield response from using three sequential sprays was approximately two times greater in the subtropics (319 to 532 kg/ha) than in the tropics (149 to 241.3 kg/ha). No significant decline in fungicide efficacy or yield response was observed in 9 years of study for any of the fungicides. These results reinforce the need to improve control by adopting an integrated management approach in the tropics given poorer performance and lower profitability, especially for the premixes, than in the subtropics.

Accuracy of static computer-assisted implant placement in long span edentulous area by novice implant clinicians: A cross-sectional in vitro study comparing fully-guided, pilot-guided, and freehand implant placement protocols.

BACKGROUND: To ensure accurate implant placement, surgical guides are used to control the steps of implant placement surgery. PURPOSE: Evaluation of the accuracy of implant placement in long span edentulous area by novice implant clinicians according to fully-guided (FG), pilot-guided (PG), and freehand (FH) placement protocols. MATERIALS AND METHODS: Maxillary surgical models with four missing teeth from the right first canine to the first molar were produced by 3-dimensional printing. Fourteen clinicians new to implant dentistry participated in the study, and each one of them inserted one canine and one molar implant for every implant placement protocol. All implant placement steps were completed in phantom heads to simulate the clinical situation. To evaluate the accuracy, the implant vertical, horizontal platform, horizontal apex, angle, and interimplant distance deviations from the planned positions were calculated. RESULTS: With the exception of vertical deviation, the FG placement was clearly more accurate than the PG and FH placements for all the variables for canine and molar implants. The PG placement was significantly more accurate than the FH placement for the horizontal platform and apex deviations, and interimplant distance deviation. The FG placement did not show a significant impact of the location of the implant, or the horizontal deviations of the platform or the apex. The PG and FH placements showed increased deviation at the canine implant than the molar implant, and at the apex of the implants than the platform of the implants. CONCLUSIONS: Within the limitations of this in vitro study, novice clinicians achieved a significantly more accurate implant position with FG placement, followed by PG and FH placements respectively. Therefore, a form of guided surgery is beneficial for novice clinicians.

Molecular characterization and sequence analysis of four Brazilian rice stripe necrosis virus isolates.

Rice (Oryza sativa L.) is an important food crop for humanity, being cultivated in tropical and temperate regions of the world. This study reports the nearly complete genome sequences of four Brazilian rice stripe necrosis virus (RSNV) isolates. The nucleotide sequences of the RNA1 and RNA2 genome segments of these Brazilian isolates were 96.5 to 99.9% identical, indicating their close phylogenetic relationship to each other. Phylogeny and recombination analysis indicated that the genome of one of the isolates consisted of RNA segments of different origins, suggesting that a reassortment event had occurred.

Seating accuracy of implant immediate provisional prostheses fabricated by digital workflow prior to implant placement by fully guided static computer-assisted implant surgery: An in vitro study.

OBJECTIVES: Evaluation of seating accuracy of implant immediate provisional prostheses fabricated prior to fully guided static computer-assisted implant surgery (sCAIS). MATERIALS AND METHODS: Two maxillary training models were used to plan for single anterior (S-Ant) and single posterior (S-Post) implant prostheses, and a bridge (B) spanning from an anterior implant (B-Ant) to a posterior implant (B-Post). A commercial software was used to plan the implant location, design the surgical guides and design the provisional prostheses. The master models with the provisional prostheses were scanned to generate virtual master models. For each maxillary model, a total of 10 guides and 10 surgical models were produced. Following implant placements in each surgical model, the provisional prostheses were attached to the implants and were scanned to produce virtual surgical models. The virtual master and surgical models were superimposed to measure the vertical error, the proximal contact error and the proximal contact quality. RESULTS: The vertical error was greatest for the S-Post (0.41 mm), followed by B-Post (0.29 mm), B-Ant (0.26 mm) and S-Ant (0.21 mm). There was no significant difference in vertical errors among the prostheses. For the proximal contact, the S-Ant had significantly greater error (0.45 mm - 0.46 mm) than S-Post (0.15 mm) and B (0.09 mm - 0.15 mm). A similar pattern was observed for proximal contact quality. CONCLUSIONS: All prostheses were associated with errors vertically and at the proximal contacts. Therefore, the clinicians who plan to use this workflow should be prepared to adjust the prosthesis after implant insertion.

Lessons Learned From Introducing Staff to Quality Improvement Through Systematic Training at the Boston Public Health Commission.

CONTEXT: The widespread adoption of quality improvement (QI) in public health departments holds the promise of transformational change for the American public health system; however, there is a lack of published literature pertaining to organization-level experiences with introductory QI training programs for health department staff. This practice report aims to share the innovative approach, experience, and results of introducing staff to QI principles at the Boston Public Health Commission (BPHC). PROGRAM: The BPHC is an accredited health department that introduced more than 80% of its 1100 staff members to QI between January 2015 and December 2018. The commission's QI training program benefited from (1) visible support from senior leaders, (2) well-documented QI training plans, (3) innovative QI training curricula, (4) flexible delivery methods, and (5) a responsive monitoring and evaluation system. METHODS: To assess the effectiveness of QI training at the BPHC, participant questionnaires from QI training sessions (run between January 2015 and June 2018) and a separate questionnaire on organizational QI culture (administered to the supervisory staff in May 2018) were assessed using the Kirkpatrick model of training evaluation. EVALUATION: On the basis of Kirkpatrick's 4 levels of training effectiveness, 91% of participants were satisfied with training content (level 1), 94% of participants found the facilitators effective (level 2), 84% of participants indicated they would apply QI concepts in everyday work (level 3), and 75% of supervisors reported an organization-wide improvement in QI culture (level 4). DISCUSSION: These results show that the BPHC's QI training program has been resoundingly effective, with positive outcomes across all 4 levels of the Kirkpatrick model. These results reinforce the idea of the BPHC's experience as an exemplar for introductory QI training. Interested health departments should heed the lessons learned from this and other organizational experiences as they attempt to scale up QI competencies among staff members.

Are Demethylation Inhibitor Plus Quinone Outside Inhibitor Fungicide Premixes During Flowering Worthwhile for Fusarium Head Blight Control in Wheat? A Meta-Analysis.

Fusarium head blight (FHB), caused mainly by Fusarium graminearum, is best controlled with demethylation inhibitor (DMI) fungicides during flowering. However, the use of premixes of DMI and quinone outside inhibitor (QoI) fungicides to control FHB has increased in Brazil. Data on FHB severity and wheat yields measured in field experiments conducted in Brazil were gathered from both peer- and nonpeer-reviewed sources published from 2000 to 2018. After selection criteria were applied, 73 field trials from 35 bibliographic sources were identified, among which 50% of the data were obtained from cooperative network trials conducted after 2011. To be included in the analysis, DMI plus QoI premixes or tebuconazole were tested in at least 14 trials and 3 years. Four premixes met the criteria. Estimates of percent control (and respective 95% confidence intervals) by a network model fitted to the log of the treatment means ranged from 44.1% (pyraclostrobin plus metconazole applied once; 32.4 to 53.7) to 64.3% (pyraclostrobin plus metconazole; 58.4 to 69.3); the latter did not differ from tebuconazole (59.9%; 53.6 to 65.3). Yield response was statistically similar for pyraclostrobin plus metconazole (532.1 kg/ha; 441 to 623) and trifloxystrobin plus prothioconazole (494.9 kg/ha; 385 to 551), and both differed statistically from a group composed of tebuconazole (448.2 kg/ha; 342 to 554), trifloxystrobin plus tebuconazole (468.2 kg/ha; 385 to 551), azoxystrobin plus tebuconazole (462.4 kg/ha; 366 to 558), and pyraclostrobin plus metconazole applied once (413.7 kg/ha; 308 to 518). The two categories of FHB index (7% cutoff) and yield (3,000 kg/ha cutoff), both in the nontreated check, did not explain the heterogeneity in the estimates. Considering only the fungicide effects on yield, two sequential sprays of tebuconazole or one spray of pyraclostrobin plus metconazole as management choices are likely more profitable than DMI plus QoI premixes sprayed twice during flowering.

Effect of Manufacturing Technique on the Accuracy of Surgical Guides for Static Computer-Aided Implant Surgery.

PURPOSE: This study aimed to evaluate the effect of different surgical guide manufacturing techniques on the accuracy of the surgical guides produced prior to implant placement. MATERIALS AND METHODS: Three type of guides were compared: milled guides using a commercial milling unit (C-Mill), printed guides using a commercial 3D printer (C-Print), and printed guides using a benchtop printer (B-Print). All the guides were fabricated on a single maxillary model for anterior implant and posterior implant placement. Ten guides were produced for each group. Four accuracy variables were measured: (1) internal accuracy, (2) vertical fit, (3) guide seating distortion, and (4) drilling access horizontal and vertical deviations. All the variables were virtually measured by 3D rendering software. The Kruskal-Wallis and the Mann-Whitney U tests were conducted to evaluate the significance of the differences among the guide groups. RESULTS: The C-Mill guides were significantly more accurate than the other guide groups for all the accuracy variables (P < .05). The C-Print group tended to show greater accuracy than the B-Print group, except for guide seating distortion and horizontal deviation of drilling access. However, a significant difference between the C-Print and B-Print groups was observed only for the vertical deviation of the posterior drilling access (P < .05). The location of the implant seemed to have minimal influence on the drilling access accuracy, except for the vertical deviations of C-Mill and C-Print guides, where the anterior site was associated with significantly (P < .05) greater errors than the posterior site. CONCLUSION: Producing guides by milling was more accurate and less vulnerable to seating distortion in comparison to printing. Despite the overall similarity between the two printers, the commercial printer tended to produce guides with greater accuracy than the benchtop printer.

Accuracy of static computer-assisted implant placement in anterior and posterior sites by clinicians new to implant dentistry: in vitro comparison of fully guided, pilot-guided, and freehand protocols.

BACKGROUND: One of the challenges encountered by clinicians new to implant dentistry is the determination and controlling of implant location. This study compared the accuracy of fully guided (FG) and pilot-guided (PG) static computer-assisted implant placement (sCAIP) protocols against the conventional freehand (FH) protocol for placing single anterior and posterior implants by recently introduced clinicians to implant dentistry. MATERIAL AND METHODS: Ten clinicians new to implant dentistry inserted one anterior (central incisor) and one posterior (first molar) implants per protocol in training maxillary models. The FG protocol involved drilling and implant placement through the guide, while the PG protocol controlled the pilot drilling only. The FH implant placement was completed without the aid of any guide. A total of 30 models were used, and 60 implants were inserted. The implant vertical, horizontal neck, horizontal apex, and angle deviations from planned positions were calculated. RESULTS: The FG protocol provided the most accurate implant placement in relation to horizontal neck (0.47 mm-0.52 mm), horizontal apex (0.71 mm-0.74 mm), and angle deviations (2.42o-2.61o). The vertical deviation was not significantly different among the different protocols. The PG protocol was generally similar to the FH protocol with a horizontal neck deviation of 1.01 mm-1.14 mm, horizontal apex deviation of 1.02 mm-1.35 mm, and angle deviation of 4.65o-7.79o. The FG protocol showed similarity in the accuracy of the anterior and posterior implants. There was a tendency for inferior accuracy for posterior implants compared with anterior implants for the PG and FH protocols. CONCLUSIONS: In the hands of recently introduced clinicians to implant dentistry, it appears that the accuracy of the FG protocol was superior to the other protocols and was not influenced by the position of the implants. The PG and FH protocols showed inferior accuracy for posterior implants compared with anterior implants.

Genetic variability and population structure of the New World begomovirus Euphorbia yellow mosaic virus.

The emergence of begomoviruses (whitefly-transmitted viruses classified in the genus Begomovirus, family Geminiviridae) in Brazil probably occurred by horizontal transfer from non-cultivated plants after the introduction of Bemisia tabaci MEAM1. The centre of diversity of Euphorbia heterophylla (Euphorbiaceae) is located in Brazil and Paraguay, where it is an invasive species in soybean and other crops. Reports of possible begomovirus infection of E. heterophylla in Brazil date back to the 1950s. In 2011, Euphorbia yellow mosaic virus (EuYMV) was described in symptomatic plants collected in the Brazilian state of Goiás. Here we assess the genetic variability and population structure of begomoviruses infecting E. heterophylla in samples collected throughout nine Brazilian states from 2009 to 2014. A total of 158 and 57 haplotypes were compared in DNA-A and DNA-B datasets, respectively. Analysis comparing population structure in a large sampled area enabled us to differentiate two subpopulations. Further, the application of discriminant analysis of principal components allowed the differentiation of six subpopulations according to sampling locations and in agreement with phylogenetic analysis. In general, negative selection was predominant in all six subpopulations. Interestingly, we were able to reconstruct the phylogeny based on the information from the 23 sites that contributed most to the geographical structure proposed, demonstrating that these polymorphisms hold supporting information to discriminate between subpopulations. These sites were mapped in the genome and compared at the level of amino acid changes, providing insights into how genetic drift and selection contribute to maintain the patterns of begomovirus population variability from a geographical structuring point of view.

Interaction between the New World begomovirus Euphorbia yellow mosaic virus and its associated alphasatellite: effects on infection and transmission by the whitefly Bemisia tabaci.

The majority of Old World monopartite begomoviruses (family Geminiviridae) are associated with satellite DNAs. Alphasatellites are capable of autonomous replication, but depend on the helper virus for movement, encapsidation and transmission by the insect vector. Recently, Euphorbia yellow mosaic alphasatellite (EuYMA) was found in association with Euphorbia yellow mosaic virus (EuYMV) infecting Euphorbia heterophylla plants in Brazil. The geographical range of EuYMA was assessed in a representative sampling of E. heterophylla plants collected in several states of Brazil from 2009 to 2014. Infectious clones were generated and used to assess the phenotype of viral infection in the presence or absence of the alphasatellite in tomato, E. heterophylla, Nicotiana benthamiana, Arabidopsis thaliana and Crotalaria juncea. Phenotypic differences of EuYMV infection in the presence or absence of EuYMA were observed in A. thaliana, N. benthamiana and E. heterophylla. Symptoms were more severe when EuYMV was inoculated in combination with EuYMA in N. benthamiana and E. heterophylla, and the presence of the alphasatellite was determinant for symptom development in A. thaliana. Quantification of EuYMV and EuYMA indicated that EuYMA affects the accumulation of EuYMV during infection on a host-dependent basis. Transmission assays indicated that EuYMA negatively affects the transmission of EuYMV by Bemisia tabaci MEAM1. Together, these results indicate that EuYMA is capable of modulating symptoms, viral accumulation and whitefly transmission of EuYMV, potentially interfering with virus dissemination in the field.

Quantitative Review of the Effects of Triazole and Benzimidazole Fungicides on Fusarium Head Blight and Wheat Yield in Brazil.

Triazole and benzimidazole fungicides have been used for controlling Fusarium head blight (FHB) in wheat for over two decades. In Brazil, it was only during the last five years that uniform fungicide trials for FHB control have been established yearly, thus contributing to a new large body of fungicide efficacy data for this country. A systematic review of both peer- and non-peer-reviewed studies on chemical control conducted since 2000 in Brazil was performed. Fungicides of interest were the triazoles tebuconazole (TEBU1x and TEBU2x) and propiconazole (PROP2x), and the benzimidazole carbendazim (CARB2x). Most fungicides were applied twice, the first at early to mid-flowering and the second 7 to 10 days later. Only TEBU was tested as one or two applications, and thus four treatments were evaluated. For these fungicides, there were 35 trials reporting FHB index and 48 reporting mean yield. Network meta-analytic models were fitted to the data of the log of the means of FHB index for each fungicide and for the nontreated check. The meta-analytic estimates were used to obtain control efficacy ( C¯ ), or percent disease reduction relative to the nontreated check. The absolute mean difference ( D¯ ) in yield (kg/ha) between the fungicide-treated and the nontreated check plots was also estimated. Yield response relative to the nontreated check ( Y¯ ) was also calculated based on the difference in the logs of the means of yield between fungicide-treated and nontreated check. The TEBU1x, TEBU2x, and CARB2x treatments performed similarly with regards to control efficacy (= 59%, 53% and 55%, respectively), and although better than PROP2x (47%), the difference was marginally significant. Yield response ( D¯ ) was highest for TEBU2x, ( D¯ = 558 kg/ha, Y¯ = 19.2%) followed by PROP2x (497 kg/ha, 16.0%), TEBU1x (457 kg/ha, 17.3%), and CARB2x (456 kg/ha, 12.8%). For an average 2016 scenario of fungicide plus application costs (FC = $18 U.S./ha) and average wheat price (WP = $215 U.S./MT), the probability of breaking even on the financial investment in the four treatments ranged from 59 to 63%. For 140 scenarios (four fungicides) created based on the combination of five WP ($133 to 266 U.S./MT) and seven FC ($5 to 35 U.S./ha), the probability of breaking even was >50% for all but two scenarios. The information may serve as a guide for planning future trials and provides a baseline and first step toward optimizing FHB management in Brazil.

Development of methodologies for virus detection in soybean and wheat seeds.

Seeds that contain large amounts of oil, starch, fibers and phenols are the most difficult tissues for RNA extraction. Currently, there are some reports of virus detection in seeds using commercial kits for RNA extraction. However, individual seeds were used, which may not be always suitable for analyses that deal with large amounts of seeds. Sangha [1] described a simple, quick and efficient protocol for RNA extraction and downstream applications in a group of seeds of jatropha (Jatropha curcas), mustard (Brassica sp.) and rice (Oryza sativa). We tested this protocol for soybean (Glycine max), maize (Zea mays), wheat (Triticum aestivum) and triticale (×Triticosecale) seeds and further reverse transcription PCR (RT-PCR)/quantitative real-time PCR (qPCR) in order to have a faster and more practical method for virus detection from seeds than the traditional scheme of seed planting and subsequent Elisa/RT-PCR from leaves. The essential points in the method are:•Some modifications in the protocol [1] were done in order to increase performance: Wheat and triticale seeds are incubated with water prior to maceration. An amount of 1.2 g of dry soybean seeds is used to maceration.•RT-PCR is used for detection of Wheat streak mosaic virus from wheat seeds and RT-qPCR for detection of Soybean mosaic virus from soybean seeds.•The method may be tested for other viruses, however, pre-validation will be needed.

Resultados agronômicos e qualitativos da nova cultivar de trigo 'BRS Marcante' / Agronomic and qualitative results of the new wheat cultivar 'BRS Marcante'

A cultivar de trigo 'BRS Marcante' foi desenvolvida pela Embrapa, envolvendo um híbrido F1 do cruzamento entre as linhagens PF 980533 e PF 970227 com a cultivar 'BRS Guamirim', realizado em telado, na Embrapa Trigo, no inverno de 2003. As gerações segregantes foram conduzidas pelo método genealógico e a linhagem resultante, nomeada de PF 080310. A cultivar caracteriza-se pela sua ampla capacidade de adaptação às condições de cultivo do sul do Brasil, pelo bom potencial de rendimento de grãos e qualidade industrial da classe Pão.

The wheat cultivar 'BRS Marcante' was developed by Embrapa, as a result a cross between F1 hybrid between lines PF 980533 and PF 970227 with 'BRS Guamirim' and carried out in a green-house of Embrapa Wheat, on 2003 winter season. The segregate generations were conducted by genealogic method and the genotype resulted was named PF 080310. It has wide adaptation ability to south Brazilian conditions, high grain yield potential and belongs to Bread Class.