Environmental and land use controls of microplastic pollution along the gravel-bed Ain River (France) and its "Plastic Valley".

Understanding microplastic particles (MPs) accumulation and transport along rivers represents a major task due to the complexity and heterogeneity of rivers, and their interactions with their wider corridor. The identification of MPs hotspots and their potential sources is especially challenging in coarse-bed rivers transporting a wide range of particle sizes with a high degree of variability in time and space. This research focuses on the gravel-bed Ain River (Rhône River tributary, France) which is managed by means of various dams and also hosts one of the major plastic production centres in Europe (Oyonnax and Bienne Plastic Valleys). In this research, (i) Geographical Information Systems (GIS) were used to locate plastic factories and to characterise the land use of the Ain River watershed. (ii) On the field, sediment samples were extracted from the hyporheic zone (HZ) of mobile gravel bar heads, while hydro-sedimentary settings were measured in order to describe site conditions. Sampling sites were especially established in downwelling areas (i.e. where the surface water entered the hyporheic zone), upstream and downstream of dams and plastic factories. (iii) After density separation and organic matter digestion of sediment, MPs were characterised with a µFTIR device followed by data processing via the siMPle software. This work highlighted the trapping efficiency of alluvial bars for MPs. The highest MPs concentrations were found along the Plastic Valleys (up to 4400 MPs/kg), while the lower river was less contaminated by MPs. After grain-size correction, a significant breakpoint was identified in the area of the main dams, revealing their major influence on MPs distribution. The variability in MPs concentrations and types suggested a local origin for most of MPs. A particular feature was the dominance of polypropylene (PP) which appears as a critical industrial heritage as the studied region is specialised in the manufacturing of hard plastics. Indeed, multivariate analyses also revealed that MPs concentrations and types were mostly driven by the vicinity of plastic factories and urban areas. This relationship between the land use, the presence of dams and MPs characteristics provides key results for the MPs assessment and the improvement of management issues along coarse-bed rivers.

Narrative review of application of metagenomic approaches to study the link between oropharyngeal microbiome and infectious diseases.

Context: Viral and bacterial infections are major causes of morbidity and mortality worldwide. The oropharyngeal microbiome could play an important role in preventing invasion of viral and bacterial pathogens by modulating its content and the host's innate immune response. Next Generation Sequencing (NGS) technologies now enable in-depth study of the genomes of microbial communities. The objective of this review is to highlight how metagenomics has contributed to establish links between changes in the oropharyngeal microbiome and emergence of bacterial and viral diseases. Method: Two search engines, PubMed and Google scholar were used with filters to focus searches on peer-reviewed original articles published between January 2010 and September 2022. Different keywords were used and only articles with metagenomic approaches were included. Results: This review shows that there were few articles studying the link between oropharyngeal microbiome and infectious diseases. Studies on viruses using metagenomic techniques have been growing exponentially in recent years due to the Covid-19 pandemic. This review shows that most studies still focus on the basic identification of microorganisms in different disease states and multiple microorganisms (Alloprevotella, Prevotella, Bacteroides, Haemophilus, Streptococcus, Klebsiella sp., Acinetobacter sp…), have been associated with development of infections such as childhood wheezing, influenza, Covid-19, pneumonia, meningitis, and tuberculosis. Conclusion: The oropharyngeal microbiome, despite its importance, remains poorly studied. A limited number of articles were identified but this number has increased exponentially since 2020 due to research conducted on Covid-19. These studies have shown that metagenomic has contributed to the unbiased identification of bacteria that could be used as biomarkers of various diseases and that further research is now needed to capitalize on those findings for human health benefit.

Impact of vaccination on the symptoms of hospitalised patients with SARS-CoV-2 Delta variant (B.1.617.1) infection.

OBJECTIVES: The diffusion of the SARS-CoV-2 Delta (B.1.617.2) variant and the waning of immune response after primary Covid-19 vaccination favoured the breakthrough SARS-CoV-2 infections in vaccinated subjects. To assess the impact of vaccination, we determined the severity of infection in hospitalised patients according to vaccine status. METHODS: We performed a retrospective observational study on patients hospitalised in 10 centres with a SARS-CoV-2 infection (Delta variant) from July to November 2021 by including all patients who had completed their primary vaccination at least 14 days before hospital admission and the same number of completely unvaccinated patients. We assessed the impact of vaccination and other risk factors through logistic regression. RESULTS: We included 955 patients (474 vaccinated and 481 unvaccinated). Vaccinated patients were significantly older (75.0 [63.25-84.0] vs. 55.0 [38.0-73.0]; p < 0.001), more frequently males (55.1% (261/474) vs. 46.4% (223/481); p = 0.009), and had more comorbidities (2.0 [1.0-3.0] vs. 1.0 [0.0-2.0]; p < 0.001). Vaccinated patients were less often admitted for Covid-19 (59.3% (281/474) vs. 75.1% (361/481); p < 0.001), had less extended lung lesions (≤25%: 64.3% (117/182) vs. 38.4% (88/229); p < 0.001), required oxygen less frequently (57.5% (229/398) vs. 73.0% (270/370); p < 0.001), at a lower flow (3.0 [0.0-8.7] vs. 6.0 [2.0-50.0] L/min, p < 0.001), and for a shorter duration (3 [0.0-8.0] vs. 6 [2.0-12.0] days, p < 0.001)., and required less frequently intensive care unit admission (16.2% (60/370) vs. 36.0% (133/369); p < 0.001) but had comparable mortality in bivariate analysis (16.7% (74/443) vs. 12.2% (53/433); p = 0.075). Multivariate logistic regression showed that vaccination significantly decreased the risk of death (0.38 [0.20-0.70](p = 0.002), ICU admission (0.31 [0.21-0.47](p < 0.001) and oxygen requirement (0.16 [0.10-0.26](p < 0.001), even among older patients or with comorbidities. CONCLUSIONS: Among patients hospitalised with a delta variant SARS-CoV-2 infection, vaccination was associated with less severe forms, even in the presence of comorbidities.

Extraction and quantification of pesticides and metals in palm wines by HS-SPME/GC-MS and ICP-AES/MS.

In this study, HS-SPME/GC-MS and ICP-AES/MS methods are presented to extract and quantify pesticides and metals in palm wines. Various parameters affecting the extraction were investigated: SPME fiber, equilibrium and extraction time, extraction temperature, salinity, and stirring, through an experimental design with 45 trials. The developed method allowed to identify 35 pesticides and quantify 29 of them, from different families of pesticides in 32 palm wine samples. Method performance was evaluated in terms of linearity, repeatability, LOD, LOQ, and accuracy. Among the 32 samples analyzed in 3 replicates, 7 pesticides were detected in 10 samples. Dichlorvos was the only pesticide detected at levels above the European maximal limits. Additionally, 10 of the 19 metals explored by ICP-AES and ICP-MS were found in all samples. Six metals were detected in different samples at levels above the European or OIV maximal limits for drinking water or wine.

Starmerella reginensis f.a., sp. nov. and Starmerella kourouensis f.a., sp. nov., isolated from flowers in French Guiana.

Analysis of yeasts isolated from various biotopes in French Guiana led to the identification of two strains isolated from flowers and designated CLIB 1634T and CLIB 1707T. Comparison of the D1/D2 domain of the large subunit (LSU D1/D2) rRNA gene sequences of CLIB 1634T and CLIB 1707T to those in the GenBank database revealed that these strains belong to the Starmerella clade. Strain CLIB 1634T was shown to diverge from the closely related Starmerella apicola type strain CBS 2868T with a sequence divergence of 1.34 and 1.30 %, in the LSU D1/D2 rRNA gene and internal transcribed spacer (ITS) sequences respectively. Strain CLIB 1634T and Candida apicola CBS 2868T diverged by 3.81 and 14.96 % at the level of the protein-coding gene partial sequences EF-1α and RPB2, respectively. CLIB 1707T was found to have sequence divergence of 3.88 and 9.16 % in the LSU D1/D2 rRNA gene and ITS, respectively, from that of the most closely related species Starmerella ratchasimensis type strain CBS 10611T. The species Starmerella reginensis f.a., sp. nov. and Starmerella kourouensis f.a., sp. nov. are proposed to accommodate strains CLIB 1634T (=CBS 15247T) and CLIB 1707T (=CBS 15257T), respectively.

Whiptail Stingray Injury.

Stingray injuries to the chest are rare but potentially life-threatening. They may occur in remote areas where advanced emergency healthcare services are unavailable. We describe the case of a 24-year-old man with tension pneumothorax due to a Himantura fai stingray injury to the left chest. The chest wound was unremarkable, with no external bleeding or evidence of a foreign body. Decompression was performed at the scene with an improvised knife procedure and a hollow writing pen, which served as a chest tube. At the local hospital, a standard-sized chest tube was inserted, the wound cleaned, and the patient given antibiotics active against marine organisms. Computed tomography visualized the stinger and revealed hemopneumothorax and pneumomediastinum. The local hospital did not have a thoracic surgeon, and the patient was transferred to a larger hospital with a thoracic surgery center. After surgical removal of the stinger, segmental lung resection was required to control bleeding. Management of life-threatening stingray injuries to the chest should begin at the scene. After stabilization, the patient should be transferred to a hospital equipped for cardiovascular and thoracic surgery. Surgery may be required to remove the retroserrated stinger and can be challenging.