RESUMEN
The paper shows that a chronic slowly developing inflammation manifesting itself as adipokine imbalance and lipid metabolism abnormalities in the body under the influence of unfavorable environmental factors.
Asunto(s)
Proteína C-Reactiva/metabolismo , Citocinas/metabolismo , Exposición a Riesgos Ambientales/efectos adversos , Enfermedades Ambientales/complicaciones , Inflamación/etiología , Metabolismo de los Lípidos/efectos de los fármacos , Enfermedades Ambientales/inducido químicamente , Enfermedades Ambientales/metabolismo , Humanos , Inflamación/metabolismoRESUMEN
It was shown in experiments on rabbits that dimethoxyphosphates of rare-earth elements injected intravenously displayed a marked anticoagulant activity. The highest effect was produced by compounds of neodymium, holmium, terbium, europium, thulium, dysprosium, and erbium.
Asunto(s)
Anticoagulantes/farmacología , Metales de Tierras Raras/farmacología , Fosfatos/farmacología , Animales , Coagulación Sanguínea/efectos de los fármacos , Conejos , Tromboelastografía , Factores de TiempoAsunto(s)
Virus Oncogénicos/crecimiento & desarrollo , Replicación Viral , Animales , Fraccionamiento Celular , Centrifugación por Gradiente de Densidad , Retículo Endoplásmico/ultraestructura , Humanos , Cuerpos de Inclusión/análisis , Cuerpos de Inclusión/ultraestructura , Cuerpos de Inclusión Viral , Ratones , Neoplasias Experimentales/microbiología , Neoplasias Experimentales/ultraestructura , Hibridación de Ácido Nucleico , Virus Oncogénicos/aislamiento & purificación , Virus Oncogénicos/ultraestructura , ADN Polimerasa Dirigida por ARN/metabolismoRESUMEN
In the cytoplasms of chick embryo fibroblast and Ehrlich ascitic carcinoma cells infected with influenza virus (fowl plague virus), in addition to fragmented virus nucleocapsid larger nucleocapsid structures were found which sedimented in the region of 90 -120S. The structures were detected upon short 3H-uridine label of the cells. Their buoyant density in cesium chloride was higher than that of the fragmented nucleocapsid (1.34 -1.39 g/cm3). In electron microscope, the structures were visualized as thin nonhelical filaments 3.5 nm in diameter, their morphology being no different from that of a similar rapidly sedimenting structure isolated from the nucleoplasms of the same cells. To determine the possibility of transfer of the rapidly sedimenting structure from the nucleus into the cytoplasm, a cell-free system was used containing nuclei from influenza virus-infected cells labeled with 3H-uridine for 5 min, as well as the cytoplasm from uninfected and unlabeled cells. The presence of a labeled rapidly sedimenting structure in the cytoplasm of the cell-free system suggests that the structure is synthesized in the nucleus and then transported into the cytoplasm. The relation of this structure to the fragmented nucleocapsid is unknown. It may be assumed to be its intracellular precursor.
Asunto(s)
Cápside , Orthomyxoviridae/crecimiento & desarrollo , ARN Viral , Proteínas Virales , Replicación ViralRESUMEN
Virus-specific structures with sedimentation coefficients of 250-300, 200 and 150 S were isolated from the polysome fraction of Sendai virus-infected Ahrlich ascitic carcinoma cells treated with cycloheximide, at early stages of infection (1.5 to 2 hours after inoculation). All these 3 types of structure contained both parental and newly synthesized viral RNA. RNA extracted from these structures consisted of 2 components sedimenting in sucrose density gradients in the zones of 50-70 and 35-40 S. Both components contained parental and newly synthesized RNA and were partially resistant to ribonuclease. RNA extracted from rapidly sedimenting structures (250-300 S) contained mainly the 50-70 S component; RNA recovered from 200 S structures contained the 35-40 S component. By analogy with reported data, the isolated forms of RNA have been characterized as transcriptive intermediates.
Asunto(s)
Carcinoma de Ehrlich/metabolismo , Virus de la Parainfluenza 1 Humana/metabolismo , ARN Viral , Animales , Técnicas de Cultivo , Cicloheximida/farmacología , Citoplasma/metabolismo , Virus de la Parainfluenza 1 Humana/análisis , Polirribosomas/metabolismo , ARN Viral/análisis , ARN Viral/biosíntesis , Ribonucleasas/metabolismo , Transcripción GenéticaRESUMEN
Transcriptive complex in the cytoplasm of Sendai virus infected cells included parental RNA in the form of ribonucleoprotein, nascent RNA and polysomes. Its buoyant density in CsC1 was 1.45 g/ml. The complex dissociated three hours after the infection, and nascent RNA was fully separated from the parental RNP. The template for polysomes within the complex was identified under conditions when the complex was dissociated in cycloheximide-treated cells. Polysomes were revealed in the association with nascent RNA. They sedimented in preribosmal region of sucrose gradient, and had a buoyant density in CsC1 of 1.49 g/ml. Mild treatment with ribonuclease split the polysomes into monoribosomes.
Asunto(s)
Nucleoproteínas/metabolismo , Virus de la Parainfluenza 1 Humana , Polirribosomas/metabolismo , ARN Viral/metabolismo , Ribonucleoproteínas/metabolismo , Citoplasma/metabolismo , Ribonucleasas , Moldes Genéticos , Transcripción GenéticaRESUMEN
In the cell cytoplasm of human tissue cultures Detroit-6 and AO which produce B type oncorna-virus, two types of virus-specific structures were revealed. Structures of type I were aggregated fibrils of 3 and 6 nm diametre. Structures of type II were nucleoids of A-particles of 70-80 nm diametre. They were rather well separated from cell components by centrifugation sucrose density gradient and repeated centrifugation in the sucrose concentration gradient. Fibrils were found in the density regions of the equilibrium gradient of 1.26 and 1.19 g/cm3, whereas A-particles were detected in the sones of the density of 1.29 and 1.23-124 g/cm3. Their sedimentation coefficients in the sucrose concentration gradients were about 150S and 250S, respectively. From both structure types similar RNA classes were extracted sedimenting in 60S, 45S and 35S regions (sucrose concentration gradient). In addition, 20S RNA was found within the 150S structures. Both structures sa. However, hydridization degree of RNA isolated from both structures with DNA synthesized enzymatically on extracellular various (DNA I) and A-particles (DNA II) was different. With DNA-I, 50-80% of RNA isolated from the type I structures and less than 20% of RNA extracted from the type II structures were hybridized. At the same time, strictly opposite situation (50-80% of RNA II and 20% of RNA I) was observed for DNA-II. These data show lack of genetic connection between these types of cytoplasmic structures and possible role of type I structures in reproduction of oncorna-virus type B.
Asunto(s)
Virus Oncogénicos/ultraestructura , Células Cultivadas , Centrifugación por Gradiente de Densidad , Humanos , Microscopía Electrónica , Peso Molecular , Hibridación de Ácido Nucleico , Virus Oncogénicos/análisis , ARN Viral/análisisRESUMEN
A particles with the diameter of 70 to 80 nm were isolated from the cytoplasm of HEp-2, HeLa, and AO cells producing oncornavirus of Mason-Pfizer-like type. Most of the A particles banded at 1.23 to 1.24 g/ml, whereas 3 to 10% banded at 1.29 g/ml in equilibrium sucrose gradients. They banded at 1.30 g/ml in CsCl gradients suggesting that they contained 8% RNA. Individual A particles sedimented at 200 to 250S in velocity sucrose gradients, but their significant part was found aggregated and sedimented at more than 300S. They were resistant to RNase digestion. A particles possessed polymerase activity which was preferentially activated by Mn(2+) rather than by Mg(2+), the RNA template being 60S RNA. Cross-hybridization with two DNA products and immunoassay showed that A particles and Mason-Pfizer-like oncornavirus produced by the same cells contained neither homological RNA sequences nor common antigens, suggesting that A particles are not intracellular precursors of Mason-Pfizer-like oncornavirus but represent an independent oncornavirus. Hybridization of A particle RNA with excess of cellular DNA revealed about 20 proviral copies per HEp-2 cell genome and no proviral copies in human embryo and placenta cell genomes.
Asunto(s)
Células Cultivadas/microbiología , Citoplasma/microbiología , Cuerpos de Inclusión Viral , Virus Oncogénicos , Antígenos Virales/análisis , Carcinoma de Células Escamosas , Fraccionamiento Celular , Línea Celular , Centrifugación por Gradiente de Densidad , ADN Viral/biosíntesis , Células HeLa , Humanos , Inmunodifusión , Neoplasias Laríngeas , Pulmón/embriología , Microscopía Electrónica , Hibridación de Ácido Nucleico , Virus Oncogénicos/enzimología , Virus Oncogénicos/inmunología , Virus Oncogénicos/aislamiento & purificación , Radioisótopos de Fósforo , ARN Viral , ADN Polimerasa Dirigida por ARN/metabolismo , Moldes Genéticos , Tritio , UridinaAsunto(s)
Línea Celular/microbiología , Cuerpos de Inclusión Viral , Carcinoma de Células Escamosas/patología , Fraccionamiento Celular , Centrifugación por Gradiente de Densidad , Técnicas Citológicas , Citoplasma/ultraestructura , ADN Viral/análisis , ARN Polimerasas Dirigidas por ADN/análisis , Células HeLa/ultraestructura , Humanos , Hibridación Genética , Inmunodifusión , Neoplasias Laríngeas/patología , Microscopía Electrónica , Virus Oncogénicos/ultraestructura , Virus ARN/ultraestructura , ARN Viral/análisisAsunto(s)
Nucléolo Celular/análisis , Nucleoproteínas/biosíntesis , Virus de la Parainfluenza 1 Humana/crecimiento & desarrollo , Proteínas Virales/biosíntesis , Animales , Carcinoma de Ehrlich , Células Cultivadas , Centrifugación Zonal , Cicloheximida/farmacología , Dactinomicina/farmacología , Microscopía Electrónica , ARN Viral/biosíntesisRESUMEN
Two kinds of virus-specific structures were isolated from the cytoplasm of Detroit-6 and human amnion cells producing oncornavirus-like particles. These structures represented A particles with the diameter of 70 to 80 nm and aggregated strands of nucleocapsids with the diameter of 3 and 6 nm. The structures were separated from cellular contaminants by isopycnic banding in linear sucrose gradients and subsequently further purified by sedimentation in velocity sucrose gradients. Their sedimentation coefficient was 250 and 150S, respectively. Both structures contain 60, 45, and 35S RNA species, and 150S structures also contained 20S RNA. The 35 and 20S RNA from the 150S structure formed hybrids with DNA enzymatically synthesized on extracellular virions. The structures displayed endogeneous polymerase activity, DNA product of the reaction being predominantly associated with 60S RNA. No 70S RNA was found in the cell structures of various densities. Also, the virions purified from tissue culture fluid contained 70S RNA. These findings are consistent with those on extracellular maturation of oncornavirus RNA.