RESUMEN
The present study aimed to examine the anti-inflammatory effects and potential mechanism of action of Artemisia asiatica Nakai (A. asiatica Nakai) extract in activated murine macrophages. A. asiatica Nakai extract showed dose-dependent suppression of lipopolysaccharide (LPS)-induced nitric oxide, inducible nitric oxide synthase, and cyclooxygenase-2 activity. It also showed dose-dependent inhibition of nuclear factor-κB (NF-κB) translocation from the cytosol to the nucleus and as an inhibitor of NF-κB-alpha phosphorylation. The extract's inhibitory effects were found to be mediated through NF-κB inhibition and phosphorylation of extracellular signal-regulated kinase 1/2 and p38 in LPS-stimulated J774A.1 murine macrophages, suggesting a potential mechanism for the anti-inflammatory activity of A. asiatica Nakai. To our knowledge, this is the first report of the anti-inflammatory effects of A. asiatica Nakai on J774A.1 murine macrophages; these results may help develop functional foods possessing an anti-inflammatory activity.
Asunto(s)
Artemisia/química , Macrófagos/inmunología , FN-kappa B/metabolismo , Extractos Vegetales/farmacología , Transducción de Señal/efectos de los fármacos , Proteínas Quinasas p38 Activadas por Mitógenos/metabolismo , Animales , Antiinflamatorios/farmacología , Ciclooxigenasa 2/metabolismo , Lipopolisacáridos/inmunología , Lipopolisacáridos/toxicidad , Macrófagos/efectos de los fármacos , Ratones , Óxido Nítrico/metabolismo , Óxido Nítrico Sintasa de Tipo II/metabolismo , Fosforilación/efectos de los fármacos , Transducción de Señal/inmunologíaAsunto(s)
Antineoplásicos/farmacología , Antioxidantes/farmacología , Taurina/farmacología , Benzotiazoles/química , Bioensayo , Compuestos de Bifenilo/química , Cromanos/química , Ensayos de Selección de Medicamentos Antitumorales , Depuradores de Radicales Libres/farmacología , Humanos , Células MCF-7 , Picratos/química , Ácidos Sulfónicos/química , Taurina/química , Células Tumorales CultivadasAsunto(s)
Neoplasias de la Mama/patología , Movimiento Celular/efectos de los fármacos , Taurina/farmacología , Neoplasias de la Mama/genética , Línea Celular Tumoral , Movimiento Celular/genética , Estradiol/farmacología , Femenino , Regulación Neoplásica de la Expresión Génica/efectos de los fármacos , Humanos , Células MCF-7 , Metaloproteinasa 9 de la Matriz/genética , Neoplasias Hormono-Dependientes/genética , Neoplasias Hormono-Dependientes/patología , Inhibidor Tisular de Metaloproteinasa-1/genética , Inhibidor Tisular de Metaloproteinasa-2/genética , Factor A de Crecimiento Endotelial Vascular/genéticaRESUMEN
uvi31+ is a sequence homolog of Escherichia coli bolA gene in Schizosaccharomyces pombe, identified as a UV-inducible gene. Here, the cellular function of uvi31+ was investigated by null mutant analysis. Deletion of uvi31+ led to a delayed germination of spore and defects in subsequent cell division. However, the uvi31 mutant cell proliferated faster with smaller cell size than the wild-type cell during vegetative growth. In addition, the uvi31 mutant was sensitive to UV-light. It showed a normal cell cycle delay after UV-irradiation but displayed aberrant septum formation and defective cytokinesis when released from the UV damage checkpoint. These results suggest that uvi31+ may be involved in control of cell division, especially during the resumption from cell cycle arrest.