RESUMEN
AIM: The aim of this study was to develop a novel artificial nerve conduit and to evaluate its efficiency based on the promotion of peripheral nerve regeneration in rabbits. MATERIAL AND METHODS: The nerve conduit was made of a poly (l-lactide-co-glycolic acid)-coated collagen tube filled with collagen gel. The conduits were implanted into a 15 mm gap in the peroneal nerves of five rabbits. On the contralateral side, the defects were bridged with collagen-filled vein grafts. RESULTS: Twelve weeks postoperatively nerve regeneration was superior to the vein graft in the PLGA-coated collagen tube, both morphologically and electrophysiologically. CONCLUSION: The results indicate the superiority of the PLGA-coated collagen tube over vein grafts. Furthermore, they show that entubulation repair with this type of tube can support nerve regeneration over a nerve gap distance of at least 15 mm.
Asunto(s)
Materiales Biocompatibles , Materiales Biocompatibles Revestidos , Colágeno , Regeneración Tisular Dirigida/instrumentación , Ácido Láctico , Regeneración Nerviosa/fisiología , Ácido Poliglicólico , Polímeros , Potenciales de Acción/fisiología , Animales , Axones/ultraestructura , Materiales Biocompatibles/química , Materiales Biocompatibles Revestidos/química , Colágeno/química , Estimulación Eléctrica , Electromiografía , Diseño de Equipo , Ácido Láctico/química , Masculino , Músculo Esquelético/inervación , Músculo Esquelético/fisiología , Fibras Nerviosas Mielínicas/ultraestructura , Nervio Peroneo/fisiopatología , Nervio Peroneo/cirugía , Nervio Peroneo/ultraestructura , Ácido Poliglicólico/química , Copolímero de Ácido Poliláctico-Ácido Poliglicólico , Polímeros/química , Conejos , Tiempo de Reacción/fisiología , Factores de Tiempo , Venas/trasplanteRESUMEN
OBJECTIVES: The aim of this in vitro study was to evaluate the suitability of using chitosan, poly (lactide-co-glycolide) (PLGA), and polymethyl methacrylate (PMMA) to control the release of chlorhexidine digluconate (CHX) from a prototype of controlled release drug device for root canal disinfection. STUDY DESIGN: Four different prototypes with different formulations were prepared. Group A (n = 12): the device (absorbent paper point) was loaded with CHX as control. Group B (n = 12): same as group A, but the device was coated with chitosan (Texan MedTech). In Groups C and D, the device was treated in the same way as group A and then coated 3 times with 5% PMMA (Group C, n = 12, Aldrich), or coated 3 times with 3% PLGA (Group D, n = 12, Sigma). The devices were randomly allocated to experimental groups of 12 each. All the prototypes of controlled release drug device were soaked in 3 mL distilled water. The concentrations of CHX were determined using a UV spectrophotometer. The surface characteristics of each prototype were observed using a scanning electron microscope. RESULTS: The result showed that release rate of CHX was the greatest in the noncoated group, followed by the chitosan-coated group, the PLGA-coated group, and the PMMA-coated group (P < 0.05). Pores were observed on the surface of the prototypes that were coated with PLGA and PMMA. When the pore size was smaller, the release rate was lower. CONCLUSION: These data indicate that polymer coating can control the release rate of CHX from the prototypes of controlled release drug device.
