The metacognitive link between co-occurring intra- and interpersonal symptoms in major depression.

OBJECTIVES: Co-occurring symptoms in depressed people currently in a relationship are sometimes considered to be due to interpersonal variables like dyadic adjustment, or intrapersonal factors such as a lack of dispositional mindfulness. Recent theories of emotional distress have, however, proposed that metacognitive beliefs could be a better link between these frequently co-occurring symptoms in major depression. METHODS: In a French sample of depressed inpatients currently in a relationship (n=30), we conducted a mediation analysis to assess whether the score of the Metacognitions Questionnaire-30 mediated the relation between on the one side the Beck depression inventory and on the other side the dyadic adjustment scale, the Beck anxiety inventory, and the five facets mindfulness questionnaire. RESULTS: By using a multiple linear regression and the Sobel test, we found that the Metacognitions Questionnaire fully mediated the association of depression severity with both anxiety and the affectionate expression of the Dyadic Adjustment Scale, and partially mediated that of the Five Facets Mindfulness Questionnaire with depression severity. CONCLUSIONS: These results obtained in a clinical sample gave support for the mediating role of dysfunctional metacognitive beliefs on some aspects of depressive symptomatology, as well as the likely metacognitive nature of important dispositional mindfulness facets. Limitations of this study are discussed, including its cross-sectional design as well as its sample size.

Sequential "electrochemical peroxidation - Electro-Fenton" process for anaerobic sludge treatment.

In this study, we present a sequential electrochemical process for integral treatment of anaerobic sludge, combining for the first time electrochemical peroxidation (ECP) and electro-Fenton (EF). In the first step, ECP (consisting of H2O2-assisted electrocoagulation with Fe electrodes) was applied as a conditioning and stabilizing method, whose synergistic electrocoagulation/Fenton oxidation effects considerably reduced the COD, TOC and total suspended solids (TSS) by 89.3%, 75.4% and 85.6%, respectively, under optimized conditions (initial pH of 5, [H2O2]/[Fe2+] dose ratio of 5, 15.38 mA cm-2 and 2 h treatment). Furthermore, total coliforms were completely killed within the first hour of treatment. In the second step, EF was successfully applied to mineralize the remaining organic fraction in the liquid effluent after dewatering, achieving 91.6% and 87.2% of COD and TOC removal, respectively, after 4 h of treatment under optimal conditions (pH 3 and 25 mA cm-2), while almost total COD and TOC removal was attained in 8 h. The Fe sludge generated at the end of the ECP treatment was easily dewatered by filtration and 20.9 g of nutrient-rich dry sludge were produced. The overall cost of the ECP-EF treatment was S$ 0.05 L-1 sludge. The combined effects of coagulation and Fenton oxidation during ECP revealed that the treatment efficiency is strongly dependent on the rheological properties of the sludge sample.

[Validation of the bias against disconfirmatory evidence in a French-speaking sample of schizophrenic patients]. / Validation du biais contre les indices infirmatoires chez un échantillon francophone de patients schizophrènes.

PURPOSE: The purpose of this study was to verify the generality of the bias against disconfirmatory evidence in a French-speaking sample of schizophrenic patients. This bias is a heightened persistence of incorrect interpretations in front of contradictions, previously demonstrated in schizophrenic patients coming from non-French-speaking communities. METHOD: In a pictorial decision task, 20 schizophrenic, 20 depressive and 20 control participants reappraised during eight successive steps, six interpretations proposed for emotionally neutral but initially incomplete pictures. Each step added a fragment of the picture represented. The reappraisal of the plausibility of incorrect interpretations between each step (change score [CS]) was measured for all the participants. The positive and negative symptoms scale as well as the scale for the assessment of positive symptoms were only administered to schizophrenic patients. RESULTS: Evolution of CS as a function of the seven last steps was significantly less pronounced in the schizophrenic samples. Only the negative dimension of the PANSS was correlated to the CS. DISCUSSION: These results replicated the BADE in a small French-speaking schizophrenic sample. The absence of a significant correlation between the BADE and the positive symptoms and measures of delusion was discussed with regard to both the small size of the sample and the explicative status of this bias.

Electro-Fenton for control and removal of micropollutants - process optimization and energy efficiency.

The removal of micropollutants is an important environmental and health issue. Electro-Fenton offers an electrochemical advanced treatment that is particularly effective for the breakdown of aromatic contaminants. Due to the wide variety of chemicals, it is preferable to analyze model contaminants, such as phenol, when optimizing and assessing the efficacy of a novel treatment process. In this study, we therefore made use of innovative types of electrode material and optimized operating parameters (current density and aeration rate) for the removal of phenol by electro-Fenton, with a view to maximize the energy efficiency of the process. By determining the best current density (1.25 mA cm-2), frequency of aeration (continuous) and by using a boron-doped diamond (BDD) anode, it was possible to achieve over 98.5% phenol (1 mM) removal within 1.5 h. BDD further outcompeted platinum as anode material in terms of mineralization rate and yield, and displayed low energy consumption of 0.08 kWh (g-TOC)-1, about one order of magnitude lower than other advanced oxidation processes, such as UV/TiO2 and UV/O3. Furthermore, a carbon cloth anode proved even more cost-effective than BDD if the end goal is the removal of phenol by electro-Fenton instead of complete mineralization.

Different types of carbon nanotube-based anodes to improve microbial fuel cell performance.

The microbial fuel cell (MFC) is an innovative technology for producing electricity directly from biodegradable organic matter using bacteria. Among all the influenceable factors, anode materials play a crucial role in electricity generation. Recently, carbon nanotubes (CNTs) have exhibited promising properties as electrode material due to their unique structural, and physical and chemical properties. In this study, the impacts of CNT types in CNT-based anodes were investigated to determine their effect on both efficiency of wastewater treatment and power generation. The CNTs, namely single-walled CNT with carboxyl group (SWCNT), multi-walled CNT with carboxyl group (MWCNT-COOH) and multi-walled CNT with hydroxyl group (MWCNT-OH) were used to fabricate CNT-based anodes by a filtration method. Overall, MWCNTs provided better results than SWCNTs, especially in the presence of the -OH groups. The highest power and treatment efficiencies in MFC were achieved with an anode made of MWCNT-OH filtered on Poreflon membrane; the open circuit voltage attained was 0.75 V and the maximum power density averaged 167 mW/m(2), which was 130% higher than that obtained with plain carbon cloth. In addition, MWCNT-OH is more cost-effective, further suggesting its potential to replace plain carbon cloth generally used for the MFC anode.

Biological treatment of pharmaceutical wastewater from the antibiotics industry.

Pharmaceutical wastewater generated by an antibiotics (penicillin) company was treated by aerobic membrane bioreactors (MBRs) and sequencing batch reactors (SBRs). At a low organic loading rate of 0.22 kg-COD m(-3)d(-1), both types of reactors were capable of treating the wastewater such that the treated effluent met the discharge regulation except for the total dissolved solids. However, when the loading rate was increased to 2.92 kg-COD m(-3)d(-1), foaming issues resulted in unstable performance. Overall, the MBRs achieved better solid removal but the SBRs performed better in regards to the degradation of aromatic compounds, as determined by UV absorbance (UVA). Finally, ozonation was applied on two different streams and showed promise on the strong stream - that corresponds to the formulation effluent and contains most of the biorefractory compounds. Ozonation successfully reduced the UVA, lowered the pH and increased the biochemical oxygen demand : chemical oxygen demand (BOD5 : COD) ratio of the strong stream. However, it was less efficient on the effluent having undergone pre-treatment by a biofilter due to a lack of selectivity towards refractory compounds.

Suitability of ozone pre-treatment for amoxicillin wastewater.

This study deals with the ozonation of amoxicillin in real pharmaceutical wastewater and its efficacy as a pre-treatment, prior to biological degradation by a mixed culture of bacteria in a sequencing batch reactor (SBR). An ozone utilization of 0.27 g/g-COD (chemical oxygen demand) lowered the pH of the wastewater to 6.6, reduced the specific ultraviolet absorption by 43% and increased the biochemical oxygen demand (BOD) concentration by 37%. The BOD:COD ratio became equal to 0.89, making the ozonated wastewater seemingly suitable for biological treatment; however, when the ozonated effluent was fed to the SBR, the reactor performance degraded, an effect which was attributed to ozonation by-products. In conclusion, ozonation might not be a suitable pre-treatment for pharmaceutical wastewater containing amoxicillin, and biotreatment with properly acclimated biomass may be a better option for treatment of such pharmaceutical wastewater.

Dysregulation of laminins in intestinal inflammation.

Laminins are structural components of basement membranes that regulate and control many cellular functions. Changes in basement membrane composition play significant roles in etiology of diseases. Inflammatory bowel diseases are conditions that lead to defects in the mucosal barrier which includes the basement membrane underlying the epithelium. This review will summarize the main findings related to the involvement of laminins and of the laminin-binding receptors in inflammatory conditions such as Crohn's disease and ulcerative colitis. We will review the current literature devoted to studies in humans (immunolocalisation, genetic factors, microarray data), as well as experimental cell models that show that laminins contribute to the inflammation process probably linked to the deregulation of proinflammatory cytokines.

Conception and optimization of a membrane electrode assembly microbial fuel cell (MEA-MFC) for treatment of domestic wastewater.

A membrane electrode assembly (MEA) for microbial fuel cells (MEA-MFC) was developed for continuous electricity production while treating domestic wastewater concurrently. It was optimized via three upgraded versions (noted α, ß and γ) in terms of design (current collectors, hydrophilic separator nature) and operating conditions (hydraulic retention time, external resistance, aeration rate, recirculation). An overall rise of power by over 100% from version α to γ shows the importance of factors such as the choice of proper construction materials and prevention of short-circuits. A power of 2.5 mW was generated with a hydraulic retention time of 2.3 h when a Selemion proton exchange membrane was used as a hydrophilic separator in the MEA and 2.8 mW were attained with a reverse osmosis membrane. The MFC also showed a competitive value of internal resistance (≈40-50 Ω) as compared to the literature, especially considering its large volume (3 L). However, the operation of our system in a complete loop where the anolyte was allowed to trickle over the cathode (version γ) resulted in system failure.

The role of the basement membrane as a modulator of intestinal epithelial-mesenchymal interactions.

Intestinal development is a process of continuous dynamic bidirectional crosstalk between epithelial and underlying mesenchymal cells. This crosstalk is mediated by well-dissected signaling pathways. Another crucial actor in the epithelio-mesenchymal interactions is the stromal microenvironment, which is composed of extracellular matrix molecules. Among them, the basement membrane (BM) molecules are secreted by the epithelium and mesenchyme in a complementary manner. These molecules signal back to the cells via the integrins or other specific receptors. In this review, we mainly focus on the BM molecules, particularly laminins. The major BM molecules are organized in a complex molecular network, which is highly variable among organs. Cell culture, coculture, and grafting models have been of great interest in understanding the importance of these molecules. Mouse gene ablation of laminin chains are interesting models, which often lead to embryonic death and are frequently accompanied by compensatory processes. Overall, the BM molecules have a crucial role in the careful maintenance of intestinal homeostasis.

T-RFLP reveals high ß-Proteobacteria diversity in microbial fuel cells enriched with domestic wastewater.

AIMS: To assess the biodiversity of a large number of microbial fuel cell (MFC) anodes from a variety of MFC designs, all enriched with domestic wastewater, using a molecular fingerprinting method. METHODS AND RESULTS: We optimized a protocol allowing the rapid characterization of MFC communities using terminal restriction fragment length polymorphism (T-RFLP) with two different sets of primers and a varying number of restriction enzymes. This protocol was further validated by direct comparison with bacterial clone libraries. Twenty-one MFC anodes were analysed by T-RFLP. We also provided a statistical comparison with other bacterial communities from environments sharing common features. CONCLUSIONS: Bacterial communities were dominated by ß-Proteobacteria, mostly belonging to the Burkholderiales order, that are known to play an active role in the cycle of metals such as iron and manganese. This property may allow them to properly pass electrons to the anode of an MFC. SIGNIFICANCE AND IMPACT OF THE STUDY: Unlike other groups, ß-Proteobacteria have seldom been acknowledged as potentially efficient electrochemically active bacteria (EAB) in MFCs. Yet, they are plentiful in natural environments like biocorrosion biofilms and acid mine drainages that consequently show some potential for MFC enrichment.

A microbial fuel cell equipped with a biocathode for organic removal and denitrification.

Microbial fuel cells (MFCs) are a promising anaerobic technology but they are limited by the high cost of the catalyst used at the cathode (typically platinum). In this study, we designed a novel type of two-chambered MFC wherein an autoheterotrophic denitrifying biofilm replaced the costly catalyst on the cathode surface. Micro-organisms performed denitrification by using electrons supplied by bacteria oxidizing domestic wastewater and acetate as substrates in the anode chamber. This two-chambered MFC equipped with a biocathode generated during more than 1.5 month up to 9.4 mW m(-2) of anode surface or 0.19 W m(-3) of anode chamber volume, while removing over 65% of COD, 84% of total nitrogen and nearly 30% of suspended solids with domestic wastewater as a substrate, and nearly 95% of acetate in the subsequent experiments.

An insight into cathode options for microbial fuel cells.

Microbial fuel cell (MFC) is an emerging and promising technology, particularly in the field of wastewater treatment. The MFC capability of achieving organic removal and generating in situ electricity could make it an attractive alternative wastewater treatment technology over conventional treatment technologies. However, MFC is still far from being economically viable, especially because of the cost of the platinum (Pt) catalyst that makes possible the reaction at the cathode. In this study, we tested alternative cathode catalysts, namely sputter-deposited Cobalt (Co) and denitrifying bacteria (biocathode). The performance of these innovative cathodes was compared with that of classic Pt-cathodes. Co competed well with Pt, but further research is still required for biocathodes. However, biocathodes MFC have showed promise.

Impact of increasing NaCl concentrations on the performance and community composition of two anaerobic reactors.

The anaerobic treatment of saline effluents using halophilic and halotolerant microbial consortia is of major interest. Inhibition of anaerobic digestion is known to occur at high salt content. However, it seems that the suitable adaptation of an anaerobic sludge makes possible the treatment of saline wastewater. In this study, a non-saline anaerobic sludge was inoculated in two anaerobic batch reactors operating with a different substrate (distillery vinasse and ethanol) and subjected to increasing NaCl concentrations. The performance of the digesters appeared to be highly dependent on the nature of the substrate, and a similar level of inhibition (i.e. around 90% of the specific loading rate and specific methanogenic activity) was stated at 10 g l(-1) of NaCl with distillery vinasse and 60 g l(-1) of NaCl with ethanol. The characterization of the microflora and its adaptation to increasing NaCl conditions were also investigated using molecular tools based on the analysis of genomic 16S rDNA. The microbial communities revealed a high diversity that could be maintained in both reactors despite the increase in NaCl concentrations.

Microbial diversity in hypersaline wastewater: the example of tanneries.

In contrast to conventional wastewater treatment plants and saline environments, little is known regarding the microbial diversity of hypersaline wastewater. In this study, the microbial communities of a hypersaline tannery effluent, and those of three treatment systems operating with the tannery effluent, were investigated using 16S rDNA phylogenetic markers. The comparative analysis of 377 bacterial sequences revealed the high diversity of this type of hypersaline environment, clustering within 193 phylotypes (> or = 97% similarity) and covering 14 of the 52 divisions of the bacterial domain, i.e. Proteobacteria, Bacteroidetes, Firmicutes, Actinobacteria, Chlorobi, Planctomycetes, Spirochaetes, Synergistes, Chloroflexi, Thermotogae, Verrucomicrobia, OP3, OP11 and TM7. Most of the phylotypes were related to halophilic and pollutant-degrading bacteria. Using statistical analysis, the diversity of this type of environment was compared to that of other environmental samples selected on the basis of their salinity, oxygen content and organic load.

Anaerobic digestion of tannery soak liquor with an aerobic post-treatment.

The leather industry occupies a place of prominence in the Indian economy due to its massive potential for employment, growth and exports. The potential environmental impact of tanning is significant. This study focuses on tannery soak liquor, generated by the soaking of hides and skins, which is characterised by high organic load and high salinity. For these reasons, the soak liquor should be segregated and pre-treated separately before being mixed with the composite wastewater, made of all other streams mixed together. The anaerobic digestion of tannery soak liquor was studied using a UASB. COD removal reached 78% at an OLR of 0.5 kg COD m(-3) d(-1), a HRT of 5 days and a TDS concentration of 71 gl(-1). The combination of the UASB with an aerobic post-treatment enhanced the performance of the overall wastewater treatment process and the COD removal efficiency of the combined anaerobic/aerobic treatment system reached 96%. However, for effective operation, the system had to be operated at very low OLRs, which affects the economic viability of such a process.

Generation of a conditionally null allele of the laminin alpha1 gene.

Laminins are heterotrimeric glycoproteins of the basement membranes. Laminin 1 (alpha1, beta1, gamma1) is the major laminin expressed during early mouse embryogenesis. To gain access to the physiological function of laminin alpha1 chain, we developed a conditionally null allele of its encoding gene (Lama1) using the cre/loxP system. Floxed-allele-carrying mice (Lama1(flox/flox)) display no overt phenotype. Lama1(flox/flox) mice were crossed with transgenic deleter mice (CMV-Cre) to generate Lama1-deficient mice (Lama1(Delta/Delta)). Lama1(Delta/Delta) embryos die during the early postimplantation period after embryonic day 6.5. They lack Reichert's membrane, an extraembryonic basement membrane in which laminin alpha1 is normally highly expressed. In parallel, Lama1(Delta/Delta) embryos display 1) parietal and visceral endoderm differentiation defects with altered expression of cytokeratin 19 and GATA4, respectively, and 2) an induction of apoptosis. This new mouse model is of particular interest as it will allow time- and tissue-specific inactivation of the Lama1 gene in various organs.

Halophilic biological treatment of tannery soak liquor in a sequencing batch reactor.

Hypersaline wastewater (i.e. wastewater containing more than 35 gl(-1) total dissolved solids (TDS)) is generated by various industrial activities. This wastewater, rich in both organic matter and TDS, is difficult to treat using conventional biological wastewater treatment processes. Among the industries generating hypersaline effluents, tanneries are prominent in India. In this study, tannery wastewater from soak pit was treated in a lab-scale SBR for the removal of organic matter. The characterisation of the soak liquor showed that this effluent is biodegradable, though not easily, and highly variable, depending on the origin and the nature of the hides. TDS was in the range of 21-57 gl(-1) and COD was in the range of 1.5-3.6 gl(-1). This soak liquor was biologically treated in an aerobic sequencing batch reactor seeded with halophilic bacteria, and the performance of the system was evaluated under different operating conditions with changes in hydraulic retention time, organic loading rate and salt concentration. The changes in salinity appeared to affect the removal of organic matter more than the changes in hydraulic retention time or organic loading rate. Despite the variations in the characteristics of the soak liquor, the reactor achieved proper removal of organic matter, once the acclimation of the microorganisms was achieved. Optimum removal efficiencies of 95%, 93%, 96% and 92% on COD, PO4 3-, TKN and SS, respectively, could be reached with 5 days hydraulic retention time (HRT), an organic loading rate (OLR) of 0.6 kg COD m(-3)d(-1) and 34 g NaCl l(-1). The organisms responsible for nitrogen removal appeared to be the most sensitive to the modifications of these parameters.

Treatment of hypersaline industrial wastewater by a microbial consortium in a sequencing batch reactor.

Hypersaline effluents are produced by various industrial activities. Such wastewater, rich in both organic matter and salt (> 35 g l(-1)), is difficult to treat by conventional wastewater treatment processes. It is necessary to use halophilic bacteria. In this study, a bench-scale sequencing batch reactor (SBR) was inoculated with halophilic sediments in order to treat an agri-food effluent containing 120 g salt l(-1). The micro-organisms were able to treat carbon and nitrogen, provided the pH in the reactor was neutralised with phosphoric acid. Soluble COD and Soluble TKN removal attained 83% and 72% respectively. 16S rDNA identification of the halophilic microbial community showed high diversity.

Obesity in relation to prostate cancer risk: comparison with a population having benign prostatic hyperplasia.

OBJECTIVE: To analyse the relationship between obesity and prostate cancer, when compared with men with benign prostatic hyperplasia (BPH). PATIENTS AND METHODS: The records were reviewed of consecutive patients with histologically confirmed prostate cancer admitted for prostate surgery between January 1993 and February 1999. Controls were selected from patients who were hospitalized at the same time for the surgical treatment of BPH. One control was matched to each case by age. Obesity was defined as a body mass index (BMI) of> 29 kg/m2. RESULTS: The study included 194 cases and 194 controls; their median (range) age at operation was 69.5 (50-88) years in both groups, and the BMI 26.1 (16.6-38.1) kg/m2 in the cancer and 25.7 (15.1-36.8) kg/m2 in the BPH group. The difference between the groups was not significant (P = 0.06). Obesity was significantly associated with prostate cancer, with an odds ratio (95% confidence interval) of 2.47 (1.41-4.34). Cases with advanced disease had a higher BMI than those with localized disease, but when age was considered the difference was not significant. CONCLUSION: In general the BMI was not significantly associated with prostate cancer when compared with men having BPH. However, obese men had 2.5 times the risk of having prostate cancer.