NaxSb Alloy-Based Low-Frequency Mechanical Energy Harvesters for Virtual Taste Sensations.

Batteries can be activated by external mechanical force and generate current, enabling a smart class of electrochemical-mechanical strain energy harvesters therefrom. Here, we have developed a NaxSb alloy-based harvester that is able to electrochemically convert low-frequency bending or pressing mechanical energy into electrical energy. The device is designed as a flexible symmetric cell incorporating two sodiated antimony nanoflake electrodes, whose peak power and energy output are more than twice those of other sodium-alloyed electrochemical-mechanical strain energy harvesters reported. We demonstrate that the open-circuit voltage of the device is an asymptotic function of the curvature radius in the bending mode and a linear function of pressure in the pressing mode. Taking advantage of the tunability of voltage, we present a new technology that simulates various tastes by releasing low-voltage electrical signals from the harvester. This technology can not only help people with impaired taste but also be integrated into a virtual reality system to create immersive taste experiences.

Multi-Omics Analysis Reveals Sphingomyelin Accumulation, Glycerolipids Loss, and Disorders of Lipid Metabolism Regulated by Leucine Deprivation in the Liver of Mice.

SCOPE: Branched-chain amino acids, especially leucine, have been reported to play a role in regulating lipid metabolism. This study aims to examine the effects of leucine deprivation on hepatic lipid metabolism. METHODS AND RESULTS: C57BL/6 mice are fed with a chow diet (control group, n = 8) or a leucine-free diet (-Leu group, n = 8) for 7 days. Histology, lipidomics, targeted metabolomics, and transcriptomics are performed to analyze the liver tissue. Compared to control group, -Leu group exhibits a notably reduced liver weight, accompanied by hepatic injury, and disorders of lipid metabolism. The level of sphingomyelin (SM) is significantly increased in the liver of -Leu group, while the glycerolipids (GL) level is significantly decreased. The expression of sphingomyelin synthase 1 (SGMS1) is upregulated by leucine deprivation in a time-dependent manner, leading to hepatic SM accumulation. Moreover, leucine deprivation results in hepatic GL loss via suppressing fatty acid synthase (FASN) and acetyl-CoA carboxylase 1 (ACC1) expression. CONCLUSION: The findings demonstrate that leucine deprivation results in abnormal lipid metabolism in the liver, mainly manifested as SM accumulation and GL loss. These results provide insights into the role of leucine in regulating lipid metabolism.

Cesium-doped ammonium vanadium bronze nanosheets as high capacity aqueous zinc-ion battery cathodes with long cycle life and superb rate capability.

Aqueous zinc-ion batteries (AZIBs) are emerging as a promising candidate for large grid energy storage due to their abundant availability and high safety. To meet long cycle life requirements, developing a stable cathode with high rate capability is of great importance. Herein, cesium-doped ammonium vanadium bronze Cs0.07NH4V4O10·0.28H2O (CNVO) is synthesized and proposed as a potential cathode material in AZIBs. The doping of Cs+ ions expands the interlayer spacing of the (001) plane from 9.7 to 10.5 Å, which leads to enhanced kinetics and a low energy barrier for the intercalation of Zn2+ ions. The as-synthesized CNVO boasts a two-dimensional (2D) sheet-like morphology with a lateral dimension of about 500 and a low thickness of 9 nm, which enables robust stability during cycling. Additionally, the synergistic interactions among Cs, NH4 and V enhance the electrochemical stability of CNVO during the Zn2+/H+ intercalation/deintercalation reactions. The three-pronged approaches make CNVO nanosheet cathodes deliver a high specific capacity of 475.6 mA h g-1 at 0.1 A g-1, a high energy density of 347.4 W h kg-1 at 74.4 W kg-1 and superb cycling stability with 90.1% capacity retention after 5000 cycles at 10 A g-1. Electrochemical studies indicate that Zn2+ ion storage in CNVO is a comprehensive process involving intercalation and pseudocapacitance, with the latter not only providing extra capacity but also facilitating fast charge-discharge capability.

Removal of a pulmonary artery foreign body during pulse ablation in a patient with atrial fibrillation: A case report.

BACKGROUND: Foreign bodies in the pulmonary circulation have been documented in the literature and are typically caused by interventional procedures. However, reports of pulmonary artery foreign bodies during femoral vein puncture are rare, and there is no description of this complication from the guidewire surface flows into the pulmonary artery during a pulse ablation in a patient with atrial fibrillation. CASE SUMMARY: We described a case in which a linear foreign body suddenly appeared on fluoroscopy image during pulsed ablation of atrial fibrillation. Multiposition angiography showed that the foreign body was currently lodged in the pulmonary artery but was hemodynamically stable. We then chose to use an interventional approach to remove the foreign body from the pulmonary artery. This foreign body was subsequently confirmed to be from the hydrophilic coating of the guidewire surface. This may be related to the difficulties encountered during the puncture of the femoral vein. This is a rare and serious complication of femoral vein puncture. Therefore, we reported this case in order to avoid a similar situation. CONCLUSION: Mismatches between interventional devices from different manufacturers used for femoral venipuncture may result in pulmonary artery foreign bodies.

RepCo: Replenish sample views with better consistency for contrastive learning.

Contrastive learning methods aim to learn shared representations by minimizing distances between positive pairs, and maximizing distances between negative pairs in the embedding space. To achieve better performance of contrastive learning, one of the key problems is to design appropriate sample pairs. In most previous works, random cropping on the input image is utilized to obtain two views as positive pairs. However, such strategies lead to suboptimal performance since the sampled crops may have inconsistent semantic information, which consequently degrades the quality of contrastive views. To address this limitation, we explore to replenish sample views with better consistency of the image and propose a novel self-supervised learning (SSL) framework RepCo. Instead of searching for semantically consistent patches between two different views, we select patches on the same image as the replenishment of positive/negative pairs, encourage patches that are similar but come from different positions as positive pairs, and force patches that are dissimilar but come from adjacent positions to have different representations, i.e. construct negative pairs to enrich the learned representations. Our method effectively generates high-quality contrastive views, explores the untapped semantic consistency on images, and provides more informative representations for downstream tasks. Experiments on adequate downstream tasks have shown that, our approach achieves ＋2.1 AP50 (COCO pre-trained) and ＋1.6 AP50 (ImageNet pre-trained) gains on Pascal VOC object detection, ＋2.3 mIoU gains on Cityscapes semantic segmentation, respectively.

Development of an online UPLC-PDA-ESI-Q-TOF-MS-LOX-FLD system for rapid screening of anti-inflammatory compounds in Polygala tenuifolia Willd.

In this study, the first ultra-high performance liquid chromatography-photo-diode array-electrospray ionization-quadrupole-time-of-flight-mass spectrometry-lipoxygenase-fluorescence detector (UPLC-PDA-ESI-Q-TOF-MS-LOX-FLD) online system was developed for the identification and evaluation of anti-inflammatory active ingredients in Polygala tenuifolia Willd. Using this system, the UPLC fingerprints, mass fragments and LOX-binding peak profiles in the samples were rapidly and simultaneously obtained. A total of 101 compounds were isolated and identified and 38 compounds (11 oligosaccharide esters, nine xanthones, 17 saponins, and one glycosyloxyflavone) showed strong LOX-binding activity. Six compounds were selected to study their LOX-binding ability, and the results indicated that the content of the six compounds had a good linear relationship with the LOX-binding ability, and it was found that the substitution position, the type of substituent and the number of glycosyl groups all had a certain influence on the LOX-binding ability of the compounds. The LOX-binding activities of 10 compounds were verified by the surface plasmon resonance (SPR) technique and the activity results were consistent with the online system. After validation, we identified 7 active compounds that combined with LOX to exert anti-inflammatory effects for the first time. All the results fully demonstrate the efficiency, stability and reliability of the online system and this work provides an exemplary and useful method for the rapid screening of potential anti-inflammatory active compounds in P. tenuifolia and other traditional Chinese medicines. At the same time, it provides a new direction for screening small molecule inhibitors of enzymes like LOX.

Frequency Spectrum Modification Process-Based Anti-Collusion Mechanism for Audio Signals.

The collusion attack combines multiple multimedia files into one new file to erase the user identity information. The traditional anti-collusion methods (which aim to trace the traitors) can defend the collusion attack, but they cannot well defend some hybrid collusion attacks (e.g., a collusion attack combined with desynchronization attacks). To address this issue, we propose a frequency spectrum modification process (FSMP) to defend the collusion attack by significantly downgrading the perceptual quality of the colluded file. The severe perceptual quality degradation can demotivate the attackers from launching the collusion attack. Because FSMP is orthogonal to the existing traitor-trace-based methods, it can be combined with the existing methods to provide a double-layer protection against different attacks. In FSMP, after several signal processing procedures (e.g., uneven framing and smoothing), multiple signals (called FSMP signals) can be generated from the host signal. Launching collusion attack using the generated FSMP signals would lead to the energy disturbance and attenuation effect (EDAE) over the colluded signals. Due to the EDAE, FSMP can significantly degrade the perceptual quality of the colluded audio file, thereby thwarting the collusion attack. In addition, FSMP can well defend different hybrid collusion attacks. Theoretical analysis and experimental results confirm the validity of the proposed method.

A new hemiacetal chromone racemate and α-glucosidase inhibitors from Ficus tikoua Bur.

From the petroleum ether and ethyl acetate portions of the 95% ethanol extract of Ficus tikoua Bur., a new hemiacetal chromone racemate, named (±)-ficunomone (1), together with twenty-two known flavonoids (2-23) were isolated. The new structure was elucidated by NMR, HRESIMS, and X-ray single-crystal diffraction analysis, and the known structures were determined by comparison of spectroscopic data with those reported from literatures. All the compounds were assayed for their inhibitory activities against yeast α-glucosidase, seven flavonoids could inhibit α-glucosidase, among which compounds 22 and 23 exhibited the highest inhibitory activity, with IC50 values at 5.12 ± 0.10 and 3.43 ± 0.15 µM respectively. Preliminary structure and relationship activity of all the compounds was analysed. Kinetic analysis of compounds 22 and 23 indicated that they are both uncompetitive inhibitors. Molecular docking studies revealed that they bound to amino acid residues of the α-glucosidase activity pocket.

Effect of Crop Rotation on Fusarium Mycotoxins and Fusarium Species in Cereals in Sichuan Province (China).

The present study was performed to evaluate the effect of crop rotation on Fusarium mycotoxins and species in cereals in Sichuan Province. A total of 311 cereal samples were randomly collected and analyzed from 2018 to 2019 in Sichuan Province. The results of mycotoxin analysis showed that the major trichothecene mycotoxins in Sichuan Province were nivalenol (NIV) and deoxynivalenol (DON), and the mean concentration of total trichothecenes (including NIV, fusarenone X [4ANIV], DON, 3-acetyldeoxynivalenol [3ADON], and 15-acetyldeoxynivalenol [15ADON]) in wheat was significantly higher than that in maize and rice. The concentration of total trichothecenes in the succeeding crops was significantly higher than that in the previous crops. In addition, wheat grown after maize had reduced incidence and concentration of trichothecene mycotoxins compared with that grown after rice, and ratooning rice grown after rice had increased incidence and concentration of trichothecene mycotoxins. Our data indicated that Fusarium asiaticum with the NIV chemotype was predominant in wheat and rice samples, while the number of the NIV chemotypes of F. asiaticum and Fusarium meridionale and the 15ADON chemotype of Fusarium graminearum in maize were almost the same. Although the composition of Fusarium species was affected by crop rotations, there were no differences when comparing the same crop rotation except for the maize-wheat rotation. Moreover, the same species and chemotype of Fusarium strains originated from different crops in various rotations, but there were no significant differences in pathogenicity in wheat and rice. These results contribute to the knowledge of the effect of crop rotation on Fusarium mycotoxins and species affecting cereals in Sichuan Province, which may lead to improved strategies for control of Fusarium mycotoxins and fungal disease in China.

ICT literacy, resilience and online learning self-efficacy between Chinese rural and urban primary school students.

In the process of large-scale online education during the COVID-19 pandemic, students' online learning has caused widespread public concerns. This study investigated the relationships between Chinese rural and urban primary school students' information communications technology (ICT) literacy, student resilience, and online learning self-efficacy in a large-scale online education environment during the pandemic in China. We compared 5,037 primary school students in rural areas to 5,045 primary school students in urban areas with matching gender and grade in nine regions in China's Guangdong province, using a survey comprising an ICT literacy scale, a student resilience scale, an online learning self-efficacy scale, and an ICT devices scale. The ICT literacy, resilience and online learning self-efficacy of primary school students in rural areas were significantly lower than those in urban areas (p < 0.01). The primary school students' ICT literacy was significantly associated with student resilience and online learning self-efficacy. Student resilience played a mediating role between ICT literacy and online learning self-efficacy, while the rural/urban area factor moderated the relationship between ICT literacy and student resilience. These findings suggest that ICT literacy is more scarce and important for primary school students in rural areas of China than for those in urban areas. Improving ICT literacy among primary school students can enhance students' resilience and thus improve their online learning self-efficacy, especially in rural areas.

Lignans and Flavonoids from Cajanus cajan (L.) Millsp. and Their α-Glucosidase Inhibitory Activities.

A pair of new lignan conformers (1-2), one new flavonoid glycoside (3), as well as nineteen known compounds were purified from the twigs and leaves of Cajanus cajan (L.) Millsp.. The planar structures of the unknown compounds were determined via NMR and high-resolution mass spectrometry, while their absolute configurations were elucidated via comparison between their experimental and calculated electronic circular dichroism (ECD) values. All the isolated compounds were assayed for their α-glucosidase inhibitory activities. The results demonstrated that compounds 8-12, 15-16, 18-19, 21-22 had strong inhibition activities, with compound 10 (IC50 =0.4±0.21 µM) most active. The structure-activity relationships were preliminarily summarized. Enzyme kinetics showed that compounds 8, 9, 15-16, 18-19, 21-22 were non-competitive inhibitors and compounds 10-12 were anti-competitive ones.

Dietary supplementation of two indigenous Bacillus spp on the intestinal morphology, intestinal immune barrier and intestinal microbial diversity of Rhynchocypris lagowskii.

This study evaluated the effects of dietary administration of two indigenous Bacillus (A: basal control diet; B: 0.15 g/kg of Bacillus subtilis; C: 0.1 g/kg of Bacillus subtilis and 0.05 g/kg of Bacillus licheniformis; D: 0.05 g/kg of Bacillus subtilis and 0.1 g/kg of Bacillus licheniformis; E: 0.15 g/kg of Bacillus licheniformis) on the digestive enzyme activities, intestinal morphology, intestinal immune and barrier-related genes relative expression levels, and intestinal flora of Rhynchocypris lagowskii. The results showed that the fold height, lamina propria width, and muscle layer thickness of midgut and hindgut in group C were significantly higher than that of group A (P < 0.05). The activities of protease, amylase, and lipase in group C were significantly higher than those of group A (P < 0.05). The relative expression levels of IL-1ß and IL-8 in the intestine of group C were significantly downregulated, and the relative expression levels of IL-10 and TGF-ß were significantly upregulated (P < 0.05). The relative expression levels of Claudin-2 in group A significantly increased and the relative expression levels of Claudin-4 in group A significantly reduced compared with other groups (P < 0.05). The relative expression levels of ZO-1 in groups C and D were significantly higher than those of other groups (P < 0.05). The Bacillus in the intestine of group C has the highest relative abundance among all groups. Overall, it can generally be concluded that dietary supplementation of indigenous Bacillus subtilis and Bacillus licheniformis (group C) can improve the intestinal morphology, digestion, and absorption enzyme activities, enhance intestinal mucosal immunity and barrier function, and maintain the intestinal microbial balance of R. lagowskii.

The immuneoreaction and antioxidant status of Chinese mitten crab (Eriocheir sinensis) involve protein metabolism and the response of mTOR signaling pathway to dietary methionine levels.

To study the effects of dietary methionine on growth performance, immunity, antioxidant capacity, protein metabolism, inflammatory response and apoptosis factors in Chinese mitten crabs (Eriocheir sinensis). Five diets with different methionine levels (0.63%, 0.85%, 1.06%, 1.25% and 1.47%) were fed to E. sinensis for 8 weeks. Results showed that in the 1.25% Met group, both growth performance and feed utilization were significantly increased. The crude protein content of crab muscle in the 1.06% and 1.25% Met groups was significantly higher than that in the control group. The immune and antioxidant enzyme activities, as well as gene expression levels of anti-lipopolysaccharide factor 1 (ALF1), Crustin-1, prophenoloxidase (proPO), cap 'n' collar isoform C (CncC) in 1.25% Met group were significantly higher than other groups. The activities of adenosine deaminase (ADA) and glutamate transaminase (GPT) in serum decreased first and then increased with the increase of methionine content, while the changes of ADA and GPT in hepatopancreas increased first and then decreased. 1.25% Met group exhibited significantly increased levels of GOT, GPT, and ADA compared to the control group. 1.25% Met diet group significantly up-regulated protein synthesis and anti-apoptotic factors, and significantly down-regulated inflammatory and pro-apoptotic factors in hepatopancreas. At 1.25% in the diet, methionine was found to boost E. sinensis growth, muscle protein deposition and immunity, as well as its antioxidant capacity. Combined with the above results, based on the expression of factors involved in the mammalian target of rapamycin (mTOR) signaling pathway and the mitogen-activated protein kinase (MAPK) signaling pathway, it is proved that methionine can not only promote protein metabolism, improve feed utilization, but also alleviate the inflammatory response and apoptosis caused by oxidative stress in the body.

Orf Virus Detection in the Saliva and Milk of Dairy Goats.

Orf is a zoonotic and highly contagious disease caused by Orf virus (ORFV) infection. Orf outbreaks in sheep and goats usually lead to high culling rate and mortality in newborn kids and lambs, posing a great threat to the development of goat and sheep industry. Human Orf occurs via direct contact with infected animals or fomites. While this disease is traditionally thought to spread through direct contact, whether other transmission routes exist remains unclear. Herein, we report the detection of ORFV in the saliva and milk of dairy goats without clinical Orf symptoms. Further analyses showed that these ORFV are infectious, as they can induce characteristic cytopathic changes in primary mammary and lip cells. Importantly, these ORFV can induce typical Orf lesions after inoculation in ORFV-free dairy goats. This is the first study showing that live, infectious ORFV can be isolated from the saliva and milk of asymptomatic goats, highlighting novel potential transmission routes of ORFV. These findings provide a novel idea for the prevention and control of Orf spread.

New Wenshen Shengjing Decoction Improves Early Embryonic Development by Maintaining Low Levels of H3K4me3 in Sperm.

BACKGROUND: New Wenshen Shengjing Decoction (NWSSJD), a traditional Chinese compound medicine, has significant effect on spermatogenesis disorder and can significantly improve sperm quality. Many components in NWSSJD can induce epigenetic modifications of different types of cells. It is not yet known whether they can cause epigenetic modifications in sperm or early embryos. OBJECTIVE: This study investigated the effect of NWSSJD on mouse early embryonic development and its regulation of H3K4me3 in mouse sperm and early embryos. METHODS: Spermatogenesis disorder was induced in male mice with CPA (cyclophosphamide). NWSSJD was administrated for 30 days. Then, the male mice were mated with the female mice with superovulation, and the embryo degeneration rate of each stage was calculated. Immunofluorescence staining was used to detect the expression of H3K4me3 in sperm and embryos at various stages. Western blotting was performed to detect methyltransferase SETD1B expression. The expressions of development-related genes (OCT-4, NANOG, and CDX2) and apoptosis-related genes (BCL-2 and p53) were measured with qRT-PCR. RESULTS: Compared with the CPA group, NWSSJD significantly reduced the H3K4me3 level in sperms, significantly increased the number of normal early embryos (2-cell embryos, 3-4-cell embryos, 8-16-cell embryos, and blastocysts) per mouse, and reduced the degeneration rate of the embryos. The expression levels of H3K4me3 and methyltransferase SETD1B in early embryos were significantly elevated by NWSSJD. Additionally, NWSSJD significantly promoted BCL-2 expression, while reducing p53 expression, thus inhibiting embryonic cell apoptosis. Moreover, the expressions of development-related genes OCT-4 and CDX2 were significantly increased by NWSSJD, but NANOG expression had no significant difference. CONCLUSION: NWSSJD may promote early embryonic development possibly by maintaining low H3K4me3 levels in sperms and normal H3K4me3 modification in early embryos and by inhibiting embryonic cell apoptosis.

Federated Continuous Learning With Broad Network Architecture.

Federated learning (FL) is a machine-learning setting, where multiple clients collaboratively train a model under the coordination of a central server. The clients' raw data are locally stored, and each client only uploads the trained weight to the server, which can mitigate the privacy risks from the centralized machine learning. However, most of the existing FL models focus on one-time learning without consideration for continuous learning. Continuous learning supports learning from streaming data continuously, so it can adapt to environmental changes and provide better real-time performance. In this article, we present a federated continuous learning scheme based on broad learning (FCL-BL) to support efficient and accurate federated continuous learning (FCL). In FCL-BL, we propose a weighted processing strategy to solve the catastrophic forgetting problem, so FCL-BL can handle continuous learning. Then, we develop a local-independent training solution to support fast and accurate training in FCL-BL. The proposed solution enables us to avoid using a time-consuming synchronous approach while addressing the inaccurate-training issue rooted in the previous asynchronous approach. Moreover, we introduce a batch-asynchronous approach and broad learning (BL) technique to guarantee the high efficiency of FCL-BL. Specifically, the batch-asynchronous approach reduces the number of client-server interaction rounds, and the BL technique supports incremental learning without retraining when learning newly produced data. Finally, theoretical analysis and experimental results further illustrate that FCL-BL is superior to the existing FL schemes in terms of efficiency and accuracy in FCL.