RESUMEN
A technique has been developed to determine free and peptide-bound hydroxyproline in blood serum. It is based on the principle of chloramine oxidation of hydroxyproline and condensation of oxidation products with p-dimethylaminobenzaldehyde. Data on changes in the serum level of hydroxyproline in children with acute pneumonia and in pregnant women are presented.
Asunto(s)
Hidroxiprolina/sangre , Insuficiencia Placentaria/sangre , Neumonía/sangre , Adulto , Benzaldehídos/química , Análisis Químico de la Sangre/métodos , Niño , Preescolar , Cloraminas/química , Femenino , Humanos , Masculino , Oxidación-Reducción , Péptidos/sangre , EmbarazoRESUMEN
A procedure has been developed for simultaneous determination of the activity of a-L-fucosidase (EC 3.2.1.5 1) and the beta-D-fucosidase activity caused by beta-D-galactosidase (EC 3.2.1.23, Form IV) in serum. The paper presents data on changes in the above serum enzymatic activities in an experiment, in children with recurrent laryngotracheitis and in pregnant females with gestosis and placental insufficiency.
Asunto(s)
alfa-L-Fucosidasa/sangre , Enfermedad Aguda , Adulto , Animales , Niño , Preescolar , Femenino , Humanos , Lactante , Laringitis/enzimología , Insuficiencia Placentaria/enzimología , Preeclampsia/enzimología , Embarazo , Ratas , Recurrencia , Suero , Estereoisomerismo , Traqueítis/enzimología , alfa-L-Fucosidasa/químicaAsunto(s)
Líquidos Corporales/metabolismo , Laringitis/metabolismo , Laringoestenosis/metabolismo , Ácidos Siálicos/análisis , Traqueítis/metabolismo , Niño , Preescolar , Femenino , Humanos , Laringitis/diagnóstico , Laringitis/terapia , Laringoestenosis/diagnóstico , Laringoestenosis/terapia , Masculino , Traqueítis/diagnóstico , Traqueítis/terapiaRESUMEN
A method has been developed for simultaneous determination of the activity of alpha-L-fucosidase (EC 3.2.1.51) and the beta-D-fucosidase activity induced by beta-D-galactosidase (IV, EC 3.2.1.23) in the serum. The paper presents data on changes in these enzymatic activities in the serum in an experiment, in acute hematogenous osteomyelitis and recurrent laryngotracheitis in children in pregnant females with gestosis and placental insufficiency.
