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1.
Health Qual Life Outcomes ; 21(1): 101, 2023 Aug 29.
Artículo en Inglés | MEDLINE | ID: mdl-37644485

RESUMEN

BACKGROUND: Eating behavior primarily depends on eating patterns which are largely influenced by interactions between physiology, environment, psychology, culture and socio-economic status. This study was designed to translate and validate the Eating Behavior Pattern Questionnaire (EBPQ) among Malaysian women. METHODS: A cross-sectional study involving translation and validation of the English version of EBPQ. The original questionnaire, contained 51 items extracted into six domains was translated in Malay using forward and backward translation, pre-tested and validated among conveniently sampled female healthcare personnel. Vegetarians, pregnant ladies and women in confinement were excluded due to special daily dietary plans. Construct validity, reliability and feasibility were analyzed using Exploratory Factor Analysis (EFA) and Confirmatory Factor Analysis (CFA). RESULTS: During translation, item modifications were made and subjected to field testing among 394 women. The original questionnaire was used as a reference to identify the positioning of items in constructs. Fifteen items were removed due to poor correlation with items within constructs. Seven factors were extracted using Varimax rotation with Kaiser-Meyer-Olkin (KMO) value range from 0.725-0.872 and significant Bartlett's test of Sphericity (p < 0.001). The item-loading of the items within the constructs ranged between 0.415-0.812 (explained variation = 62.7%). Cultural and lifestyle behavior was relabeled to lifestyle and behavioral eating, and snacking on sweets was relabeled as snacking pattern. Emotional eating was divided into two sub-factors as snacking behavior and emotional influence. CFA resulted with an acceptable fit with no presence of floor and ceiling effects. Intra-class correlation coefficient (ICC) for all the constructs were reported good and excellent. The overall internal consistency was reported as good. CONCLUSION: The modified 36-item Malay-EBPQ had moderate internal consistency, reliable and fit with multi-dimensional measures of eating behaviors and dietary patterns among women in the multi-racial population with cultural diversity.


Asunto(s)
Conducta Alimentaria , Calidad de Vida , Embarazo , Femenino , Humanos , Estudios Transversales , Malasia , Reproducibilidad de los Resultados
2.
BMC Health Serv Res ; 23(1): 627, 2023 Jun 13.
Artículo en Inglés | MEDLINE | ID: mdl-37312146

RESUMEN

BACKGROUND: Admission to an intensive care unit (ICU) is a stressful experience for patients and their family members. While the focus of management is primarily on medical care, there can be other areas which are overlooked. The purpose of this study was to investigate the needs and experiences of ICU patients and family members. METHOD: This qualitative study involved four trained researchers conducting in-depth interviews (IDI) based on a semi-structured interview guide. The participants were ICU patients and family members. All IDIs were audio-recorded and transcribed verbatim. Four researchers independently analyzed the data via thematic analysis with the aid of QDA Miner Lite®. The themes and subthemes were generated and confirmed by literature and expert opinion. RESULTS: Six IDIs were conducted with three patients and three family members, whose ages ranged from 31 to 64 years old. One pair of participants consisted of a patient and his respective family member, while the other four participants did not have a familial relationship with each other. Three main themes emerged from the analysis: (I) critical care services; (II) physical spaces; and (III) monitoring technology. Medical, psychological, physical, and social needs for critical care services were expressed by both patients and family members. Patients' needs in clinical spaces were highlighted as a conducive ICU environment with ambient temperature and controlled noise levels. In non-clinical spaces, family members expressed a need for more chairs in the waiting area. Participants expressed the need for call bells as well as patients' negative perceptions of medical equipment alarms in the ICU when it pertained to monitoring technology. CONCLUSION: This study provides an in-depth view at the needs and experiences of ICU patients and family members who have a variety of unmet needs. This understanding is critical for guiding ICU personnel and stakeholders in their efforts to humanize ICU care.


Asunto(s)
Enfermedad Crítica , Unidades de Cuidados Intensivos , Humanos , Adulto , Persona de Mediana Edad , Malasia , Centros de Atención Terciaria , Enfermedad Crítica/terapia , Familia
4.
BMC Musculoskelet Disord ; 23(1): 400, 2022 Apr 28.
Artículo en Inglés | MEDLINE | ID: mdl-35484524

RESUMEN

BACKGROUND: Chronic pain has a major impact on a patient's quality of life, affecting physical and psychological functioning. It has debilitating consequences on social and economic aspects too. This study aimed to explore the status of health-related quality of life (HRQoL) of Malaysian patients suffering from chronic non-malignant pain. METHODS: Four hospitals offering pain clinic services were involved in this multicentre cross-sectional study conducted between June and September 2020. Adult patients who had been diagnosed with non-malignant chronic pain lasting for at least three months and able to communicate in English or Malay language were recruited in this study. Participants were informed about the study and were made aware that their participation was entirely voluntary. A battery of questionnaires consists of the EuroQol-5 dimensions-5 levels questionnaire (EQ-5D-5L) and the EuroQol visual analogue scale (EQ VAS), the Pain Self-Efficacy questionnaire (PSEQ) and the Pain Catastrophizing Scale (PCS) were self-administered by the patients. Besides, a structured questionnaire was used to collect their socio-demographic information, pain condition, sleep quality and working status. Participants' usage of pain medications was quantified using the Quantitative Analgesic Questionnaire (QAQ). RESULTS: A total of 255 patients participated in this study. A median EQ-5D index value of 0.669 (IQR: 0.475, 0.799) and a median EQ VAS score of 60.0 (IQR: 50.0, 80.0) were recorded. Malay ethnicity (Adj. B: 0.77; 95% CI: 0.029, 0.126; p = 0.002) and a higher level of self-efficacy (Adj. B: 0.008; 95% CI: 0.006, 0.011; p < 0.001) were predictors of a better HRQoL, while suffering from pain in the back and lower limb region (Adj. B: -0.089; 95% CI: - 0.142, - 0.036; p = 0.001), the use of a larger amount of pain medications (Adj. B: -0.013; 95% CI: - 0.019, - 0.006; p < 0.001), and a higher degree of pain magnification (Adj. B: -0.015; 95% CI: - 0.023, - 0.008; p < 0.001) were associated with a poorer HRQoL. CONCLUSIONS: These findings suggested that Malay ethnicity and a higher level of self-efficacy were predictors of a better HRQoL in patients with chronic pain, whereas pain-related factors such as higher usage of medication, specific pain site and pain magnification style were predictors of poorer HRQoL.


Asunto(s)
Dolor Crónico , Calidad de Vida , Adulto , Dolor Crónico/diagnóstico , Estudios Transversales , Humanos , Calidad de Vida/psicología , Encuestas y Cuestionarios , Escala Visual Analógica
5.
J Pharm Policy Pract ; 15(1): 4, 2022 Jan 11.
Artículo en Inglés | MEDLINE | ID: mdl-35016728

RESUMEN

BACKGROUND: During the early phase of the COVID-19 pandemic, antibiotic usage among COVID-19 patients was noted to be high in many countries. The objective of this study was to determine the prevalence of antibiotic usage and factors affecting antibiotic usage among COVID-19 patients during the early phase of the COVID-19 pandemic in Malaysia. METHODS: This was a cross-sectional study that involved reviewing medical records of COVID-19 Malaysian patients aged 12 and above who were diagnosed with COVID-19 and received treatment in 18 COVID-19 hospitals from February to April 2020. A minimum sample of 375 patients was required. A binary logistic regression analysis was performed to determine factors associated with antibiotic usage. Variables with p < 0.05 were considered statistically significant. RESULTS: A total of 4043 cases were included for analysis. The majority of the patients (87.6%) were non-smokers, male (65.0%), and had at least one comorbidity (37.0%). The median age was 35 years (IQR: 38). The prevalence of antibiotic usage was 17.1%, with 5.5% of them being prescribed with two or more types of antibiotics. The most frequent antibiotics prescribed were amoxicillin/clavulanic acid (37.8%), ceftriaxone (12.3%), piperacillin/tazobactam (13.3%), azithromycin (8.3%), and meropenem (7.0%). Male patients (adjusted OR 1.53), who had a comorbidity (adjusted OR 1.36), associated with more severe stage of COVID-19 (adjusted OR 6.50-37.06), out-of-normal range inflammatory blood parameters for neutrophils, lymphocytes, and C-reactive protein (adjusted OR 2.04-3.93), corticosteroid use (adjusted OR 3.05), and ICU/HDU admission (adjusted OR 2.73) had higher odds of antibiotic use. CONCLUSIONS: The prevalence of antibiotic usage in the early phase of the COVID-19 pandemic was low, with amoxicillin/clavulanic acid as the most common antibiotic of choice. The study showed that clinicians rationalized antibiotic usage based on clinical assessment, supported by relevant laboratory parameters.

6.
QJM ; 115(6): 374-380, 2022 Jun 07.
Artículo en Inglés | MEDLINE | ID: mdl-34051098

RESUMEN

BACKGROUND/INTRODUCTION: Cardio-cerebral infarction (CCI), which involves the simultaneous occurrence of acute ischaemic stroke and acute myocardial infarction, has a reported incidence of 0.0009%. Treatment of CCI presents a dilemma to physicians as both conditions are time critical. Despite the need for standardized treatment protocols, published data are sparse. AIM: We aimed to summarize the reported cardio-cerebral infarction cases in the literature. DESIGN: Meta-analysis. METHODS: Four databases, Pubmed, Embase, Scopus and Google Scholar were searched until 25 August 2020. A title and abstract sieve, full-text review and extraction of data were conducted independently by three authors. RESULTS: A total of 44 cases of CCI were identified from 37 case reports and series; 15 patients (34.1%) were treated using percutaneous coronary intervention (PCI) with stent, 8 patients (18.2%) were treated with a PCI without stent, 10 patients (22.7%) were treated via a cerebral vessel thrombectomy and 8 patients (18.2%) were treated via a thrombectomy of a coronary vessel. For medications, 20 patients (45.5%) were treated with thrombolytics, 10 patients (22.7%) were treated with anticoagulants, 8 patients (18.2%) were treated with antiplatelets and 11 patients (25.0%) were treated with anticoagulants and antiplatelets. Of 44 patients, 10 patients died, and 9 of those were due to cardiac causes. Among the 44 patients, days to death was observed to be a median of 2.0 days (interquartile range (IQR): 1.5, 4.0). The modified Rankin Score was measured in nine patients, with a median score of 2.0 (IQR: 1.0, 2.5) being reported. DISCUSSION/CONCLUSION: The condition of CCI has substantial morbidity and mortality, and further studies are needed to examine the optimal diagnostic and treatment strategies of these patients.


Asunto(s)
Isquemia Encefálica , Intervención Coronaria Percutánea , Accidente Cerebrovascular , Anticoagulantes/uso terapéutico , Isquemia Encefálica/complicaciones , Infarto Cerebral/etiología , Infarto Cerebral/terapia , Humanos , Intervención Coronaria Percutánea/efectos adversos , Accidente Cerebrovascular/etiología , Resultado del Tratamiento
7.
Breast ; 23(2): 142-51, 2014 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-24393617

RESUMEN

BACKGROUND: Neoadjuvant chemotherapy has a sound rationale for use in women with large operable breast cancer, and achievement of pathological complete response (pCR) is prognostic. Epirubicin and cyclophosphamide followed by docetaxel is a standard chemotherapy regimen for early breast cancer. In metastatic breast cancer the combination of gemcitabine and a taxane has shown promising results. This phase II study investigated the efficacy and safety of incorporating gemcitabine into neoadjuvant therapy. METHODS: Female patients with operable breast cancer that was clinically T2 (≥3 cm) or T3-4, N0-1, M0 were enrolled to receive 24 weeks of neoadjuvant chemotherapy using epirubicin and cyclophosphamide followed by docetaxel and gemcitabine, plus trastuzumab if HER2-positive. The primary endpoint was the pathological complete response (pCR) rate in the breast in separate HER2-negative and HER2-positive cohorts. Secondary endpoints included pCR in both the breast and axillary lymph nodes, clinical and radiological response rates, disease free survival and safety. RESULTS: 81 patients were enrolled: 63 HER2-negative and 18 HER2-positive. 67 (84%) completed all cycles of chemotherapy, and 78 (96%) proceeded to surgery. pCR was achieved by 12 (20%) patients with HER2-negative, and 9 (53%) with HER2-positive disease. At the first interim analysis, addition of prophylactic G-CSF was recommended due to excess neutropenia. The HER2-negative cohort was closed to accrual because it did not meet the pre-specified target for pCR, and the HER2-positive cohort was closed due to slow accrual. At a median follow-up of 24 months, 12 of 81 (15%) patients had experienced a relapse of their breast cancer. CONCLUSION: Neoadjuvant gemcitabine, when added to docetaxel, after epirubicin and cyclophosphamide, did not reach the pre-specified expectations for pCR rate in HER2-negative tumours. Excess neutropenia was observed, requiring growth factor support. Addition of gemcitabine to docetaxel in this schedule cannot be recommended. Australia and New Zealand Clinical Trials Registry (www.anzctr.org.au) registration number ACTRN12606000191594.


Asunto(s)
Antraciclinas/uso terapéutico , Anticuerpos Monoclonales Humanizados/uso terapéutico , Protocolos de Quimioterapia Combinada Antineoplásica/uso terapéutico , Neoplasias de la Mama/tratamiento farmacológico , Ciclofosfamida/uso terapéutico , Desoxicitidina/análogos & derivados , Epirrubicina/uso terapéutico , Terapia Neoadyuvante , Taxoides/uso terapéutico , Adulto , Anciano , Anticuerpos Monoclonales Humanizados/efectos adversos , Neoplasias de la Mama/patología , Ciclofosfamida/efectos adversos , Desoxicitidina/efectos adversos , Desoxicitidina/uso terapéutico , Supervivencia sin Enfermedad , Docetaxel , Epirrubicina/efectos adversos , Femenino , Humanos , Persona de Mediana Edad , Taxoides/efectos adversos , Trastuzumab , Resultado del Tratamiento , Gemcitabina
8.
Eur J Cancer Care (Engl) ; 21(2): 259-65, 2012 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-22146103

RESUMEN

The aim of this study was to assess general practitioner (GP) consultations with standardised patients presenting with cancer-related problems that might benefit from radiotherapy. Standardised patient scenarios were constructed with indications for radiotherapy or with side effects of radiotherapy. Six GPs consulted six standardised patients. All consultations were video recorded. Two GPs independently rated the consultation performance using the Leicester Assessment Package (LAP). Each consultation was also assessed by two radiation oncologists to assess specific decisions taken or advice offered to 'patients' in each case. The mean duration of consultations was 13 min and 55 s. Three GPs differed significantly (P < 0.025) in competencies measured by the LAP, but not as assessed by radiation oncologists. There was no significant difference in LAP scores when reviewed by scenario. However, there was significant differences in the management of the case with prostate cancer (P= 0.005) and data suggest that GPs management of different problems presented varied widely. These data are consistent with the published literature which suggests that in practice not all patients are appropriately advised or referred. There is a need for innovations to support GPs to manage patients who would benefit from radiotherapy.


Asunto(s)
Manejo de Caso/normas , Competencia Clínica/normas , Medicina Familiar y Comunitaria/normas , Neoplasias/radioterapia , Derivación y Consulta/normas , Adulto , Anciano , Actitud del Personal de Salud , Femenino , Humanos , Masculino , Persona de Mediana Edad , Simulación de Paciente , Oncología por Radiación/normas , Grabación de Cinta de Video
9.
Int J Obstet Anesth ; 20(1): 22-5, 2011 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-21123046

RESUMEN

BACKGROUND: Pregnancy is associated with alteration in sleep patterns and quality. We wished to investigate whether pregnant women have a higher likelihood of a positive Berlin Questionnaire than non-pregnant women. METHODS: Pregnant women ages 18-45 years (n=4074) presenting for delivery, and non-pregnant women ages 18-45 years (n=490) presenting for outpatient surgery provided demographic information and completed the Berlin Questionnaire evaluating self-reported snoring and daytime sleepiness. For the pregnant patients, the infant's birth weight and Apgar scores were also recorded. RESULTS: Of the 1439 patients with a positive Berlin Questionnaire, 96 were in the non-pregnant control population versus 1343 in the pregnant population (20% vs. 33%, respectively, P<0.001; odds ratio 2.0 [95% CI: 1.6-2.5]). There was a positive correlation between infant weight and a positive Berlin Questionnaire. The incidence of preeclampsia was greater (odds ratio 3.9) in the pregnant patients with a positive Berlin Questionnaire as compared with the parturients with a negative Berlin Questionnaire (odds ratio 1.1). CONCLUSION: Parturients are more likely to have a positive Berlin Questionnaire than non-pregnant women. This may indicate an increased likelihood of sleep disordered breathing.


Asunto(s)
Complicaciones del Embarazo/diagnóstico , Síndromes de la Apnea del Sueño/diagnóstico , Encuestas y Cuestionarios , Adolescente , Adulto , Puntaje de Apgar , Berlin , Estudios de Cohortes , Trastornos de Somnolencia Excesiva/epidemiología , Femenino , Humanos , Persona de Mediana Edad , Polisomnografía , Preeclampsia/epidemiología , Embarazo , Complicaciones del Embarazo/epidemiología , Estudios Prospectivos , Síndromes de la Apnea del Sueño/epidemiología , Ronquido/epidemiología , Adulto Joven
10.
Nanotechnology ; 21(33): 335705, 2010 Aug 20.
Artículo en Inglés | MEDLINE | ID: mdl-20657047

RESUMEN

We report the first photoluminescence (PL) characterization of InAs nanowires (NWs). The InAs NWs were grown on GaAs(111) B and Si(111) substrates using the Au-assisted molecular beam epitaxy (MBE) growth technique or metal-organic chemical vapor deposition (MOCVD). We compared the PL response of four samples grown under different conditions using MBE or MOCVD. High-resolution transmission electron microscopy (HRTEM) and selected area electron diffraction (SAED) patterns were utilized to determine the crystal structure and growth directions of the NWs to relate PL features to NW structural parameters. We observed mainly three PL peaks which were below, near and above InAs bandgaps, respectively. Temperature and excitation intensity dependence PL measurements were also performed to help elucidate the origins of the PL peaks of NWs. Of particular interest was a band-edge emission peak that was blue-shifted due to quantization effects of the InAs NWs, as confirmed by our calculation.

11.
Complement Ther Med ; 17(4): 216-23, 2009 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-19632549

RESUMEN

OBJECTIVES: To investigate the usage patterns of complementary and alternative medicines (CAMs), as well as dietary interventions, by South Australian people with multiple sclerosis (MS). DESIGN: Self-administered postal survey. SETTING: Questionnaire mailed to recipients of the South Australian (SA) MS Society newsletter (n=1230). MAIN OUTCOME MEASURES: Patterns of CAMs use and dietary interventions, reasons for using/not using CAMs in MS, sources of CAMs information and monthly expenditure on CAMs/dietary interventions. RESULTS: A total of 428 surveys were returned (response rate 34.8%) of which 416 met the inclusion criteria for analysis. The majority of SA people with MS who responded reported using CAMs/dietary interventions (64.7%). Respondents with tertiary education and those with mild and moderate disease reported highest CAM use. The most frequently used CAM product categories were vitamins (81.8%), essential fatty acids (80.7%) and minerals (62.5%). Commonly used herbal products included Ginkgo biloba (18.2%) and valerian (16.4%). Popular diets were the low fat (39.8%), low/no sugar (23.8%) and gluten-free (16.4%) diets. The majority of those using CAMs/dietary interventions did so concurrently with conventional treatments (72.1%). Reasons for use included: general health and well-being; to alleviate 'general' as well as specific MS symptoms such as muscle weakness, urinary or memory problems and mobility. Conventional health professionals, and friends/family, were the most common sources of information. Monthly expenditure was most commonly AUD$20-49/month. CONCLUSION: This study reports frequent use of CAM/dietary intervention amongst SA people with MS. The majority of users did so in conjunction with conventional treatments.


Asunto(s)
Terapias Complementarias/estadística & datos numéricos , Esclerosis Múltiple/terapia , Terapia Nutricional/estadística & datos numéricos , Escolaridad , Ácidos Grasos Esenciales/uso terapéutico , Femenino , Encuestas de Atención de la Salud , Humanos , Masculino , Minerales/uso terapéutico , Fitoterapia , Índice de Severidad de la Enfermedad , Australia del Sur , Encuestas y Cuestionarios , Vitaminas/uso terapéutico
12.
J Med Imaging Radiat Oncol ; 53(1): 87-91, 2009 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-19453533

RESUMEN

Kasabach-Merritt syndrome involves thrombocytopenia, which is usually severe, in association with haemangiomata. We describe two newborn infants who developed life-threatening Kasabach-Merritt syndrome but who were successfully treated. Both had failed to respond adequately to corticosteroids and alpha-interferon. They subsequently received emergency radiotherapy. Both patients had an improvement in their platelet counts. The first infant's haemangioma gradually faded during the first 3 years of life, whereas the second infant's haemangioma had resolved 2 months after radiotherapy. No significant late effects of the radiotherapy have been noted at the 8 and 5 years follow up respectively. Radiotherapy remains an important treatment method in Kasabach-Merritt syndrome when patients fail to respond to other treatments.


Asunto(s)
Hemangioma/diagnóstico , Hemangioma/radioterapia , Trombocitopenia Neonatal Aloinmune/diagnóstico , Trombocitopenia Neonatal Aloinmune/radioterapia , Enfermedad Catastrófica/terapia , Hemangioma/congénito , Humanos , Recién Nacido , Masculino , Síndrome , Resultado del Tratamiento
13.
Ann Acad Med Singap ; 37(9): 749-52, 2008 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-18989490

RESUMEN

INTRODUCTION: Splenic abscess is an uncommon clinical problem. Traditionally, the "gold standard" treatment has been splenectomy. However, there is increasing use of non-operative treatments worldwide. MATERIALS AND METHODS: A 10-year (1996-2005) retrospective review of case records from a single centre (an 800-bed general hospital) was performed. Information regarding demographics, clinical presentation, aetiological agents and management was gathered and analysed. RESULTS: There were 21 cases from 1996 to 2005. Nineteen (90%) had multiple abscesses. Disseminated melioidosis was the most common aetiological agent (15 cases, 71%). Only 3 patients underwent splenectomy. The remainder were treated conservatively with antibiotics. Almost all the patients (19, 90%) also suffered from diabetes mellitus. CONCLUSIONS: The most common aetiological agent encountered was Burkholderia pseudomallei. Diabetes mellitus may be an important co-factor in the pathogenesis of splenic abscesses. The majority of our patients were managed conservatively and splenectomy was only occasionally required.


Asunto(s)
Absceso/etiología , Bacteriemia/complicaciones , Melioidosis/complicaciones , Enfermedades del Bazo/etiología , Absceso/tratamiento farmacológico , Absceso/epidemiología , Adulto , Anciano , Antibacterianos/uso terapéutico , Bacteriemia/tratamiento farmacológico , Femenino , Hospitales Generales/estadística & datos numéricos , Humanos , Masculino , Melioidosis/epidemiología , Persona de Mediana Edad , Estudios Retrospectivos , Singapur/epidemiología , Enfermedades del Bazo/tratamiento farmacológico , Enfermedades del Bazo/epidemiología
14.
Malays J Pathol ; 29(1): 19-24, 2007 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-19105324

RESUMEN

Two duplex PCR assays were established for the detection of C. trachomatis (Ct), N. gonorrhoeae (GC), M. hominis (Mh), and U. urealyticum (Uu). These assays were used on clinical specimens obtained from women with Premature Rupture of Membrane or Post Partum Fever, from preterm infants, as well as from women with uneventful pregnancies and their babies delivered vaginally at term. The analytical sensitivity of the duplex PCR assays with internal controls incorporated is 7.0, 19.0, 5x10(3) and 7x10(2) genome copies per reaction for Ct, GC, Mh and Uu respectively. Specificity was demonstrated by the amplification of only target DNA in the presence of other organisms. Among 40 women with normal, at term, deliveries, there were 6 positives for Ct, 2 for GC and 1 for Uu. None of these women had signs of genital tract infection. The Mh/Uu PCR was positive in 11 of 40 PROM cases, with 7 women positive for Uu, 2 for Mh and 2 others for both organisms. Of 40 blood cultures taken from post-partum maternal infections, 6 were positive for Ct and 1 for Mh. Respiratory secretions from 30 premature neonates yielded 5 positives for Uu and one each for Mh and Ct. In contrast, there was only 1 positive result (for Mh) in 30 mature neonates. With 1 exception, all mycoplasma and ureaplasma positives were confirmed by culture and the concordance between paired tracheal aspirates and nasopharyngeal swabs from neonates was 96.7%. These results show the potential use of the duplex PCR assays for the diagnosis of maternal and neonatal disease caused by the four urogenital pathogens.


Asunto(s)
Chlamydia trachomatis/aislamiento & purificación , Mycoplasma hominis/aislamiento & purificación , Neisseria gonorrhoeae/aislamiento & purificación , Reacción en Cadena de la Polimerasa/métodos , Complicaciones Infecciosas del Embarazo/diagnóstico , Ureaplasma urealyticum/aislamiento & purificación , Secreciones Corporales/microbiología , ADN Bacteriano/análisis , Femenino , Gonorrea/diagnóstico , Gonorrea/microbiología , Humanos , Recién Nacido , Transmisión Vertical de Enfermedad Infecciosa , Infecciones por Mycoplasma/diagnóstico , Infecciones por Mycoplasma/microbiología , Embarazo , Complicaciones Infecciosas del Embarazo/microbiología , Sensibilidad y Especificidad , Infecciones por Ureaplasma/diagnóstico , Infecciones por Ureaplasma/microbiología
15.
J Phys Chem B ; 109(16): 7746-8, 2005 Apr 28.
Artículo en Inglés | MEDLINE | ID: mdl-16851899

RESUMEN

We report the formation of a stable superhydrophobic surface via aligned carbon nanotubes (CNTs) coated with a zinc oxide (ZnO) thin film. The CNT template was synthesized by chemical vapor deposition on an Fe-N catalyst layer. The ZnO film, with a low surface energy, was deposited on the CNT template by the filtered cathodic vacuum arc technique. Contact angle measurement reveals that the surface of the ZnO-coated CNTs is superhydrophobic with water contact angle of 159 degrees . Unlike the uncoated CNTs surface, the ZnO-coated CNTs surface shows no sign of water seepage even after a prolonged period of time. The wettability of the surface can be reversibly changed from superhydrophobicity to hydrophilicity by alternation of ultraviolet (UV) irradiation and dark storage.


Asunto(s)
Interacciones Hidrofóbicas e Hidrofílicas , Nanotubos de Carbono , Óxido de Zinc
16.
Resuscitation ; 51(3): 309-15, 2001 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-11738784

RESUMEN

The likelihood of successful defibrillation and resuscitation decreases as the duration of cardiac arrest increases. Prolonged cardiac arrest is also associated with the development of acidosis. These experiments were designed to determine whether administration of sodium bicarbonate and/or adrenaline in combination with a brief period of cardiopulmonary resuscitation (CPR) prior to defibrillation would improve the outcome of prolonged cardiac arrest in dogs. Ventricular fibrillation (VF) was induced by a.c. shock in anaesthetised dogs. After 10 min of VF, animals received either immediate defibrillation (followed by treatment with bicarbonate or control) or immediate treatment with bicarbonate or saline (followed by defibrillation). Treatment with bicarbonate was associated with increased rates of restoration of spontaneous circulation. This was achieved with fewer shocks and in a shorter time. Coronary perfusion pressure was significantly higher in NaHCO3-treated animals than in control animals. There were smaller decreases in venous pH in NaHCO3-treated animals than in controls. The best outcome in this study was achieved when defibrillation was delayed for approximately 2 min, during which time NaHCO3 and adrenaline were administered with CPR. The results of the present study indicate that in prolonged arrests bicarbonate therapy and a period of perfusion prior to defibrillation may increase survival.


Asunto(s)
Reanimación Cardiopulmonar , Paro Cardíaco/terapia , Bicarbonato de Sodio/uso terapéutico , Animales , Perros , Cardioversión Eléctrica , Epinefrina/uso terapéutico , Factores de Tiempo , Fibrilación Ventricular/terapia
17.
J Obstet Gynaecol Res ; 26(4): 271-5, 2000 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-11049237

RESUMEN

OBJECTIVES: To prospectively study the intervention rate, duration of labour, malpositions, fetal outcome, maternal satisfaction, voiding complications and adverse events in healthy primigravidae in spontaneous labour at term following epidural analgesia. METHODS: A prospective randomized study involving 55 patients in the epidural group and 68 in the control pethidine--inhalational entonox group. RESULTS: There were significantly more obstetric interventions (instrumental deliveries) in the epidural group (p < 0.01). The total duration of labour and the duration of the second stage was prolonged in the epidural group (p < 0.01). There were more malpositions at the second stage of labour in the epidural group (p < 0.02). There were no differences in fetal outcome (Apgar scores and Special Care Nursery admissions). Patients in the epidural group were consistently happier with their method of pain relief (p < 0.01). Two patients required blood patches while another 2 patients had persistent backache post epidural analgesia. CONCLUSION: Epidural analgesia in primigravidae in spontaneous labour at term led to an increased instrumental delivery rate, prolonged duration of labour, greater rate of malpositions in the second stage, increased oxytocin requirements but with no difference in fetal outcomes but with happier mothers as compared to the control group.


Asunto(s)
Analgesia Epidural , Analgesia Obstétrica , Trabajo de Parto/fisiología , Resultado del Embarazo , Adulto , Femenino , Humanos , Presentación en Trabajo de Parto , Paridad , Satisfacción del Paciente , Embarazo , Estudios Prospectivos , Factores de Tiempo , Trastornos Urinarios
18.
Anal Biochem ; 264(1): 87-97, 1998 Nov 01.
Artículo en Inglés | MEDLINE | ID: mdl-9784192

RESUMEN

Random peptide libraries are displayed on filamentous bacteriophage as fusions to either the minor coat protein, pIII, or the major coat protein, pVIII. We have devised a means of isolating the peptide displayed on a phage clone by transferring it to the N-terminus of the maltose-binding protein (MBP) of Escherichia coli encoded by malE. Transfer of a peptide sequence to monomeric MBP eliminates phage-encoded amino acids downstream of the insert peptide as well as avidity effects caused by multivalent display on phage. Peptide:MBP fusions are also easily affinity purified on amylose columns. The pMal-p2 vector was engineered to accept phage DNA encoding pIII- and pVIII-displayed peptides fused to their respective leader sequences. Both types of leader sequence were shown to target the peptide:MBP fusions to the periplasm of E. coli. A streamlined procedure for transferring peptides to MBP was applied to clones that had been isolated from a panel of pVIII-displayed peptide libraries by screening with an HIV-1-specific monoclonal antibody (Ab). By enzyme-linked immunosorbent assay, the Ab bound each of the peptide:MBP fusions and required the presence of a disulfide bridge within each peptide. Some of the peptide:MBP fusions were also analyzed using surface plasmon resonance. Thus, our study shows the value of malE fusion vectors in characterizing phage-displayed peptides.


Asunto(s)
Transportadoras de Casetes de Unión a ATP , Proteínas Portadoras/genética , Proteínas de Escherichia coli , Proteínas de Transporte de Monosacáridos , Biblioteca de Péptidos , Proteínas de Unión Periplasmáticas , Secuencia de Aminoácidos , Bacteriófagos/genética , Secuencia de Bases , Proteínas Portadoras/metabolismo , Clonación Molecular , ADN Recombinante , Escherichia coli , Vectores Genéticos , Anticuerpos Anti-VIH/inmunología , VIH-1/inmunología , Humanos , Proteínas de Unión a Maltosa , Datos de Secuencia Molecular , Reacción en Cadena de la Polimerasa , Procesamiento Proteico-Postraduccional , Señales de Clasificación de Proteína/genética , Señales de Clasificación de Proteína/metabolismo , Proteínas Recombinantes de Fusión/genética
19.
Eval Health Prof ; 21(1): 120-33, 1998 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-10183336

RESUMEN

It is generally acknowledged that alternatives such as none of the above and all of the above should be used sparingly in multiple-choice (MC) items. But the effect that all of the above has on the reliability and validity of an MC item is unclear. This study compared the results of a single-response (SRa) item format that included all of the above as the correct response to a multiple-response (MR) item format that required examinees to select all of the available alternatives for a correct response. A crossover design was used to compare the effect of formats on student performance while item content, scoring method, and student ability levels remained constant. Results indicated that the SRa format greatly distorted examinee performance by elevating their scores because examinees who recognized two or more alternatives as being correct were cued to select all of the above. In addition, the SRa format significantly reduced the reliability and concurrent validity of examinee scores. In summary, the MR format was found to be superior. Based upon new empirical evidence, this study recommends that whenever an educator wishes to evaluate student understanding of an issue that has multiple facts, the SRa format should be avoided and the MR format should be used instead.


Asunto(s)
Evaluación Educacional/métodos , Alberta , Análisis de Varianza , Humanos , Reproducibilidad de los Resultados , Estudiantes de Enfermería
20.
Percept Mot Skills ; 82(3 Pt 2): 1203-10, 1996 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-8823886

RESUMEN

The relationship between the Myers-briggs Type Indicator and Gregorc Style Delineator, and achievement was examined by administering these instruments to 259 first-year nursing students enrolled in an introductory human anatomy and physiology course. A principal component factor analysis using a varimax rotation of the scores from the two psychometric instruments, achievement examinations and an over-all grade point average indicated that each learning style from the Gregorc Style Delineator corresponds to certain traits on the Myers-Briggs Type Indicator. An individual who had a preference for the learning style of Concrete Sequential tended to have the traits of sensing and judging on the Myers-Briggs Type Indicator, while an individual who used the learning style of Concrete Random tended to have the traits of intuition and perceiving on the Myers-Briggs Type Indicator. One who had a preference for the learning style of Abstract Sequential tended to use the trait of thinking while another who used the learning style of Abstract Random tended to have the trait of feeling. The factor analysis also indicates no relationship of any scores of the traits on the Myers-Briggs Type Indicator or learning styles of the Gregorc Style Delineator with the examination scores achieved in the human anatomy and physiology course or to the students' over-all grade point average. However, factor analysis indicates that the Myers-Briggs Type Indicator traits of Judging vs Perceiving collapsed into the Sensing vs Intuition scale, and that the Gregorc Style Delineator consists of two bipolar scales that are different from those proposed by Gregorc.


Asunto(s)
Logro , Inventario de Personalidad/estadística & datos numéricos , Estudiantes de Enfermería/psicología , Adolescente , Adulto , Anatomía/educación , Educación en Enfermería , Análisis Factorial , Femenino , Humanos , Individualidad , Masculino , Persona de Mediana Edad , Fisiología/educación , Psicometría
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