[Possibilities for pathogenetic clarification of hemolytic anemias using incubation hemolysis]. / Möglichkeiten zur pathogenetischen Klärung hämolytischer Anämien mit Hilfe der Inkubationshämolyse.

The haemolysis is the uniform answer of red blood cells to detrimental effects. The method of incubation haemolysis was controlled for its evidence concerning certain groups of causes. The determination of the free haemoglobin in the plasma before incubation may be regarded as measure of haemolysis in vivo. Compensation of the Hb-release under addition of glucose indicates to such causes which are accompanied by an increased expenditure of energy, e.g. transports of ions, protein kinases, spectrin phosphorylations. The incubation with ATP is effective above all in such cases of a haemolysis in which by enzyme defects the red blood cell itself is not able to the sufficient syntheses of connections rich in energy. Some instances for causes of an increased membrane permeability for Hb suggest that the Dacie-test allows only a superficial orientation to groups of diseases.

[Oxidative damage of mice erythrocytes infected with Plasmodium berghei]. / Oxidative Schädigung an Erythrozyten der Maus unter einer Infektion mit Plasmodium berghei.

By using the model of infection with plasmodium berghei in white mice the attempt was made to explain the oxidative damages of red blood cells as a cause for haemolysis. In addition to a diminished new formation of erythrocytes there was an increased cell lysis under the impact of infection. Without any changes of the Met-Hb-percentage and of Heinz bodies an increase of GSH and GSSG could be measured. The conclusion was drawn that a damage of red blood cells caused by oxidation may occur by changing the relation of reduced tripeptides to oxidised ones.

[Effect of formate on mouse erythrocytes in vivo and in vitro]. / Der Einfluss von Formiat auf Mäuseerythrozyten in vivo und in vitro.

The effect of formiat was tested in erythrocytes of mice damaged by oxidisation. Decrease of Heinz's bodies, stabilisation of reduced glutathion, and increase of enzyme activities by referring to an example of superoxidismutase could be identified as a positive effect. Possibilities of formiat reactions in the metabolism of red blood cells are discussed.

[Formate incubation of red blood cells from glucose-6-phosphate-dehydrogenase-deficient patients]. / Formiatinkubation roter Blutzellen von Glukose-6-Phosphat-dehydrogenase-Defektträgern.

Attempts of incubating red blood cells of G6PD-defect carriers in 10 from 25 patients showed format in these blood samples is able under the elected conditions to suppress haemolysis, to reduce the numbers of Heinz's bodies and to enhance GSH-concentration. A more distinct efficiency could be observed in enzyme variants with instable temperatures and if kinetic properties had changed. Prevention of haemolysis is particularly suitable as a parameter because it will reflect all damages connected with an enhanced membrane permeability. The increase of GSH-concentration as an important equivalent of reduction in the metabolism of red blood cells may diminish the toxicity of damaging factors having an effect on oxidation. The number of Heinz's bodies is essential in those cases where oxidation products of haemoglobin are increasingly formed only under certain conditions of incubation. The findings allow a format therapy to be recommended for selected G6PD defects.